Alteration of Leaf Metabolism in Bt-Transgenic Rice (Oryza sativa L.) and Its Wild Type under Insecticide Stress

2012 ◽  
Vol 11 (8) ◽  
pp. 4351-4360 ◽  
Author(s):  
Jia Zhou ◽  
Lei Zhang ◽  
Yuwei Chang ◽  
Xin Lu ◽  
Zhen Zhu ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Transgenic Rice ◽  
Oryza Sativa L ◽  
Wild Type ◽  
Bt Transgenic Rice ◽  
Leaf Metabolism

scholarly journals Effects of Overproduction of Rubisco Activase on Rubisco Content in Transgenic Rice Grown at Different N Levels

2020 ◽  
Vol 21 (5) ◽  
pp. 1626 ◽  
Author(s):  
Mao Suganami ◽  
Yuji Suzuki ◽  
Eri Kondo ◽  
Shinji Nishida ◽  
So Konno ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Transgenic Rice ◽  
Oryza Sativa L ◽  
Rubisco Activase ◽  
Small Subunit ◽  
Wild Type ◽  
N Supply ◽  
Rubisco Small Subunit ◽  
N Concentration ◽  
Rbcs Genes

It has been reported that overproduction of Rubisco activase (RCA) in rice (Oryza sativa L.) decreased Rubisco content, resulting in declining photosynthesis. We examined the effects of RCA levels on Rubisco content using transgenic rice with overexpressed or suppressed RCA under the control of different promoters of the RCA and Rubisco small subunit (RBCS) genes. All plants were grown hydroponically with different N concentrations (0.5, 2.0 and 8.0 mM-N). In RCA overproduced plants with > 2-fold RCA content (RCA-HI lines), a 10%–20% decrease in Rubisco content was observed at 0.5 and 2.0 mM-N. In contrast, at 8.0 mM-N, Rubisco content did not change in RCA-HI lines. Conversely, in plants with 50%–60% increased RCA content (RCA-MI lines), Rubisco levels remained unchanged, regardless of N concentration. Such effects on Rubisco content were independent of the promoter that was used. In plants with RCA suppression to < 10% of the wild-type RCA content, Rubisco levels were increased at 0.5 mM-N, but were unchanged at 2.0 and 8.0 mM-N. Thus, the effects of the changes in RCA levels on Rubisco content depended on N supply. Moreover, RCA overproduction was feasible without a decrease in Rubisco content, depending on the degree of RCA production.

scholarly journals Transcription Profile Analysis of Chlorophyll Biosynthesis in Leaves of Wild-Type and Chlorophyll b-Deficient Rice (Oryza sativa L.)

Agriculture ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 401
Author(s):  
Minh Khiem Nguyen ◽  
Tin-Han Shih ◽  
Szu-Hsien Lin ◽  
Jun-Wei Lin ◽  
Hoang Chinh Nguyen ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Core Protein ◽  
Profile Analysis ◽  
Oryza Sativa L ◽  
Electron Flow ◽  
Chlorophyll Biosynthesis ◽  
Wild Type ◽  
Transcription Analysis ◽  
Chl A ◽  
Chl Biosynthesis

Photosynthesis is an essential biological process and a key approach for raising crop yield. However, photosynthesis in rice is not fully investigated. This study reported the photosynthetic properties and transcriptomic profiles of chlorophyll (Chl) b-deficient mutant (ch11) and wild-type rice (Oryza sativa L.). Chl b-deficient rice revealed irregular chloroplast development (indistinct membranes, loss of starch granules, thinner grana, and numerous plastoglobuli). Next-generation sequencing approach application revealed that the differential expressed genes were related to photosynthesis machinery, Chl-biosynthesis, and degradation pathway in ch11. Two genes encoding PsbR (PSII core protein), FtsZ1, and PetH genes, were found to be down-regulated. The expression of the FtsZ1 and PetH genes resulted in disrupted chloroplast cell division and electron flow, respectively, consequently reducing Chl accumulation and the photosynthetic capacity of Chl b-deficient rice. Furthermore, this study found the up-regulated expression of the GluRS gene, whereas the POR gene was down-regulated in the Chl biosynthesis and degradation pathways. The results obtained from RT-qPCR analyses were generally consistent with those of transcription analysis, with the exception of the finding that MgCH genes were up-regulated which enhance the important intermediate products in the Mg branch of Chl biosynthesis. These results indicate a reduction in the accumulation of both Chl a and Chl b. This study suggested that a decline in Chl accumulation is caused by irregular chloroplast formation and down-regulation of POR genes; and Chl b might be degraded via the pheophorbide b pathway, which requires further elucidation.

State of the foreign gene and of the genome in transgenic rice (Oryza sativa L.)

1993 ◽  
Vol 11 (S1) ◽  
pp. S123-S125 ◽  
Author(s):  
P. H. Bao ◽  
S. Castiglione ◽  
C. Giordani ◽  
W. Li ◽  
G. Wang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Transgenic Rice ◽  
Oryza Sativa L ◽  
Foreign Gene

A novel fungal arsenic methyltransferase, WaarsM reduces grain arsenic accumulation in transgenic rice (Oryza sativa L.)

2018 ◽  
Vol 344 ◽  
pp. 626-634 ◽  
Author(s):  
Shikha Verma ◽  
Pankaj Kumar Verma ◽  
Alok Kumar Meher ◽  
Amit Kumar Bansiwal ◽  
Rudra Deo Tripathi ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Transgenic Rice ◽  
Oryza Sativa L ◽  
Arsenic Accumulation

Functional characterisation of OsAMT1.1 overexpression lines of rice, Oryza sativa

2006 ◽  
Vol 33 (4) ◽  
pp. 339 ◽  
Author(s):  
Anshuman Kumar ◽  
Brent N. Kaiser ◽  
M. Yaeesh Siddiqi ◽  
Anthony D. M. Glass
Keyword(s):  
Oryza Sativa ◽  
Plant Biomass ◽  
Oryza Sativa L ◽  
Wild Type ◽  
High Affinity ◽  
Functional Characterisation ◽  
Overexpression Line ◽  
Net Uptake ◽  
Transgenic Lines ◽  
Responsive Gene

In rice (Oryza sativa L.) OsAMT1.1 is the most active and / or most N-responsive gene responsible for high-affinity NH4+ transport (HATS) activity. We measured 13NH4+ influx and plant biomass in transgenic overexpression lines and two wild type cultivars of rice, Jarrah and Taipei, with one or more copies of OsAMT1.1. 13NH4+ influx was higher for the overexpression lines of Jarrah line when grown at 10 µm external NH4+ concentration, but not for the overexpression lines of Taipei. For seedlings grown at 2 mm external NH4+ concentration Jarrah lines 77-1 and 75-4 showed an increased influx; however, two overexpression lines of Taipei showed reduced influx rates. The biomasses of the transgenic lines grown at low and high external NH4+ concentrations were either reduced or showed no statistically significant differences compared with wild type lines. While 13NH4+ influx into roots of Jarrah line 75-4 grown at 10 µm external NH4+ concentration was significantly higher than in wild type, measurements of 13NH efflux revealed no differences, and thus net uptake of NH4+ was higher in this overexpression line.

A TFIIIA-type zinc finger protein confers multiple abiotic stress tolerances in transgenic rice (Oryza sativa L.)

2012 ◽  
Vol 80 (3) ◽  
pp. 337-350 ◽  
Author(s):  
Ji Huang ◽  
Shujing Sun ◽  
Dongqing Xu ◽  
Hongxia Lan ◽  
Hui Sun ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Abiotic Stress ◽  
Zinc Finger ◽  
Transgenic Rice ◽  
Zinc Finger Protein ◽  
Oryza Sativa L ◽  
Finger Protein

The effects of Cd on lipid peroxidation, hydrogen peroxide content and antioxidant enzyme activities in Cd-sensitive mutant rice seedlings

2007 ◽  
Vol 87 (1) ◽  
pp. 49-57 ◽  
Author(s):  
J. Chen ◽  
C. Zhu ◽  
D. Lin ◽  
Z. -X Sun
Keyword(s):  
Lipid Peroxidation ◽  
Oryza Sativa ◽  
Antioxidant Enzyme ◽  
Oryza Sativa L ◽  
Antioxidant Enzyme Activities ◽  
Wild Type ◽  
Rice Seedlings ◽  
Sensitive Mutant ◽  
Cd Toxicity ◽  
Potential Biomarker

Cadmium-sensitive rice (Oryza sativa L. subsp. Japonica ‘Zhonghua11’) mutants were obtained using an Agrobacterium tumefaciens-based gene delivery system. Significant phenotypic differences were observed between a Cd-sensitive mutant (ST) and wild type (WT) rice seedlings. Results indicated that Cd accumulation in the leaves of the mutant was twice that of the wild type after 10 d of 0.5 mM Cd2+ treatment. Furthermore, a rapid Cd-induced H2O2 increase was observed in the mutant leaves, which induced abnormally early activity in antioxidant enzymes such as superoxide dismutase (SOD). However, the mutant leaves showed lower catalase (CAT) activity. By contrast, guaiacol peroxidase (G-POD) activities were higher in the mutant than in the wild type roots. Together with the Cd toxicity-induced decline of early responsive enzymatic activities in vivo, especially CAT, the inability of mutants to scavenge accumulated H2O2 resulted in higher lipid peroxide levels. H2O2 might also strengthen the expression of G-POD as a signaling molecule. Results suggest that G-POD activity can be a potential biomarker reflecting Cd sensitivity in rice seedlings. Key words: Antioxidant enzyme, Cd toxicity, Cd-sensitive mutant, lipid peroxidation, rice (Oryza sativa L.)

scholarly journals Senescence-Specific Expression of RAmy1A Accelerates Non-structural Carbohydrate Remobilization and Grain Filling in Rice (Oryza sativa L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Ning Ouyang ◽  
Xuewu Sun ◽  
Yanning Tan ◽  
Zhizhong Sun ◽  
Dong Yu ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Transgenic Rice ◽  
Oryza Sativa L ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Grain Filling ◽  
Leaf Sheath ◽  
Specific Expression ◽  
Total Soluble Sugar ◽  
Transgenic Lines

Remobilization of pre-anthesis NSCs (non-structural carbohydrates) is significant for effective grain filling in rice (Oryza sativa L.). However, abundant starch particles as an important component of NSCs are still present in the leaf sheath and stem at the late stage of grain filling. There are no studies on how bioengineering techniques can be used to improve the efficiency of NSC remobilization. In this study, RAmy1A was expressed under the senescence-specific promoter of SAG12, which was designed to degrade starch in the leaf sheath and stem during grain filling. RAmy1A mRNA successfully accumulated in the leaf, stem, and sheath of transgenic plants after anthesis. At the same time, the starch and total soluble sugar content in the leaf, stem, and leaf sheath were obviously decreased during the grain-filling period. The photosynthetic rate of transgenic lines was higher than that of the wild types by an average of 4.0 and 9.9%, at 5 and 10 days after flowering, respectively. In addition, the grain-filling rate of transgenic lines was faster than that of the wild types by an average of 26.09%. These results indicate an enhanced transport efficiency of NSCs from source tissues in transgenic rice. Transgenic rice also displayed accelerated leaf senescence, which was hypothesized to contribute to decreased grain weight.

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