scholarly journals Rabies virus-based COVID-19 vaccine CORAVAX™ induces high levels of neutralizing antibodies against SARS-CoV-2

npj Vaccines ◽  
2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Drishya Kurup ◽  
Christoph Wirblich ◽  
Holly Ramage ◽  
Matthias J. Schnell
Keyword(s):  
Global Health ◽  
Infectious Diseases ◽  
Rabies Virus ◽  
Causative Agent ◽  
Neutralizing Antibodies ◽  
Rapid Development ◽  
Emerging Infectious Diseases ◽  
Vaccine Platform ◽  
The World ◽  
Further Development

Abstract The recently emerged coronavirus SARS-CoV-2, the causative agent of COVID-19, is rapidly spreading in the world. The exponentially expanding threat of SARS-CoV-2 to global health highlights the urgent need for a vaccine. Herein we show the rapid development of a novel, highly efficient, and safe COVID-19 vaccine using a rabies virus-based vector that has proven to be an efficient vaccine against several emerging infectious diseases. This study reports that both a live and an inactivated rabies virus containing the SARS-CoV-2 spike S1 protein induces potent virus-neutralizing antibodies at much higher levels than seen in the sera of convalescent patients. In summary, the results provided here warrant further development of this safe and established vaccine platform against COVID-19.

scholarly journals A single dose SARS-CoV-2 simulating particle vaccine induces potent neutralizing activities

2020 ◽  
Author(s):  
Di Yin ◽  
Sikai Ling ◽  
Xiaolong Tian ◽  
Yang Li ◽  
Zhijue Xu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Neutralizing Antibodies ◽  
Vaccine Candidate ◽  
Emerging Infectious Diseases ◽  
Innate Immune ◽  
Transduction Efficiency ◽  
Vaccine Platform ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Further Development

ABSTRACTCoronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for which a vaccine is urgently needed to control its spreading. To facilitate the representation of a native-like immunogen without being infectious, here, we reported a SARS-CoV-2 vaccine candidate (designated ShaCoVacc) by incorporating spike-encoding mRNA inside and decorating spike protein on the surface of the virus simulating particles (VSPs) derived from lentiviral particles. We characterized the mRNA copy number, glycosylation status, transduction efficiency, and innate immune property of the new vaccine platform. Importantly, we showed the ShaCoVacc induced strong spike-specific humoral immune responses and potent neutralizing activities by a single injection. Additionally, we disclosed the epitopes of spike-specific antibodies using peptide microarray and revealed epitopes susceptible to specific neutralizing antibodies. These results support further development of ShaCoVacc as a candidate vaccine for COVID-19 and VSP may serve as a new vaccine platform for emerging infectious diseases.

scholarly journals One-Health: a Safe, Efficient, Dual-Use Vaccine for Humans and Animals against Middle East Respiratory Syndrome Coronavirus and Rabies Virus

2016 ◽  
Vol 91 (2) ◽  
Author(s):  
Christoph Wirblich ◽  
Christopher M. Coleman ◽  
Drishya Kurup ◽  
Tara S. Abraham ◽  
John G. Bernbaum ◽  
...  
Keyword(s):  
Middle East ◽  
Infectious Diseases ◽  
Rabies Virus ◽  
Neutralizing Antibodies ◽  
Vaccine Development ◽  
Middle East Respiratory Syndrome ◽  
Full Length ◽  
Mrna Levels ◽  
Vaccine Platform ◽  
S Protein

ABSTRACT Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in 2012 and is a highly pathogenic respiratory virus. There are no treatment options against MERS-CoV for humans or animals, and there are no large-scale clinical trials for therapies against MERS-CoV. To address this need, we developed an inactivated rabies virus (RABV) that contains the MERS-CoV spike (S) protein expressed on its surface. Our initial recombinant vaccine, BNSP333-S, expresses a full-length wild-type MERS-CoV S protein; however, it showed significantly reduced viral titers compared to those of the parental RABV strain and only low-level incorporation of full-length MERS-CoV S into RABV particles. Therefore, we developed a RABV-MERS vector that contained the MERS-CoV S1 domain of the MERS-CoV S protein fused to the RABV G protein C terminus (BNSP333-S1). BNSP333-S1 grew to titers similar to those of the parental vaccine vector BNSP333, and the RABV G–MERS-CoV S1 fusion protein was efficiently expressed and incorporated into RABV particles. When we vaccinated mice, chemically inactivated BNSP333-S1 induced high-titer neutralizing antibodies. Next, we challenged both vaccinated mice and control mice with MERS-CoV after adenovirus transduction of the human dipeptidyl peptidase 4 (hDPP4) receptor and then analyzed the ability of mice to control MERS-CoV infection. Our results demonstrated that vaccinated mice were fully protected from the MERS-CoV challenge, as indicated by the significantly lower MERS-CoV titers and MERS-CoV and mRNA levels in challenged mice than those in unvaccinated controls. These data establish that an inactivated RABV-MERS S-based vaccine may be effective for use in animals and humans in areas where MERS-CoV is endemic. IMPORTANCE Rabies virus-based vectors have been proven to be efficient dual vaccines against rabies and emergent infectious diseases such as Ebola virus. Here we show that inactivated rabies virus particles containing the MERS-CoV S1 protein induce potent immune responses against MERS-CoV and RABV. This novel vaccine is easy to produce and may be useful to protect target animals, such as camels, as well as humans from deadly MERS-CoV and RABV infections. Our results indicate that this vaccine approach can prevent disease, and the RABV-based vaccine platform may be a valuable tool for timely vaccine development against emerging infectious diseases.

scholarly journals Recent Developments towards Portable Point-of-Care Diagnostic Devices for Pathogen Detection

2022 ◽  
Author(s):  
Sharmili Roy ◽  
FAREEHA ARSHAD ◽  
Shimaa Eissa ◽  
Mohammadali Safavieh ◽  
Sanaa G. Alattas ◽  
...  
Keyword(s):  
Global Health ◽  
Infectious Diseases ◽  
Pathogen Detection ◽  
Point Of Care ◽  
Rapid Development ◽  
Diagnostic Tools ◽  
Recent Developments

The rapid development of accurate and quick diagnostic tools for infectious diseases has made a massive impact in global health. POC devices for pathogen detection have primarily contributed to clinical...

Emerging and Reemerging Infectious Diseases - Past, Present and Future

2007 ◽  
Vol 2 (2) ◽  
pp. 66-70 ◽  
Author(s):  
Yoshifumi Takeda ◽  
Keyword(s):  
Infectious Diseases ◽  
Emerging Infectious Diseases ◽  
World Health ◽  
Control Law ◽  
Global Response ◽  
The Us ◽  
The World ◽  
Us Government ◽  
Health Organization ◽  
Global Threat

The global threat of new infectious diseases first became widely recognized in the 1990s. The US government published a report on emerging and reemerging infectious diseases followed by the World Health Organization (WHO), which adopted the slogan "Emerging Infectious Diseases: Global Alert, Global Response" in 1997. Typical examples of the more than 30 infectious diseases emerging since 1970s are HIV/AIDS, Vibrio cholerae O139 infection, enterohemorrhagic Escherichia coli infection, severe acute respiratory syndrome (SARS), and avian influenza. The New Infectious Diseases Control Law enacted in Japan in 1999 was to control these emerging infectious diseases and the already existing ones.

scholarly journals Research on the channels for creation and development of high-quality “green manufacturing” brands — a case study of Xiamen

2021 ◽  
Vol 257 ◽  
pp. 03081
Author(s):  
Xiujuan Zhang ◽  
Pingyuan Zhao
Keyword(s):  
Manufacturing Industry ◽  
Rapid Development ◽  
Green Manufacturing ◽  
Design Guidelines ◽  
Ecological Impacts ◽  
High Quality ◽  
The World ◽  
Global Vision ◽  
Further Development

as the manufacturing industry gains momentum around the world, the conflicts between its rapid development and the accompanying consumption of materials, discharge of wastes and ecological impacts spiral. Governments around the world have explored measures and made achievements in alleviating the dual pressures from economic growth and environmental degradation. As for the overall trend of the manufacturing industry, creating and developing “green manufacturing” brands is the only way for the manufacturing industry to achieve further development. It is of great significance to design guidelines for green manufacturing from the angle of branding, the green notion provides more profundity and the soul to brands, ensuring sustainable development of brands. The development of green brands in Xiamen has adopted a global vision and related to the local realities, which would provide lessons for high-quality development of green manufacturing brands in other places.

scholarly journals BANK PROJECT FINANCING IN THE CONDITIONS OF MACROECONOMIC INSTABILITY IN UKRAINE

2020 ◽  
pp. 126-138
Author(s):  
Nazar DEMCHYSHAK ◽  
Oksana SHCHUREVYCH ◽  
Olha HEORHIIEVSKA
Keyword(s):  
Large Scale ◽  
Rapid Development ◽  
Market Demand ◽  
Project Financing ◽  
Short Term ◽  
Investment Projects ◽  
Long Period ◽  
The World ◽  
Banking Institutions ◽  
Further Development

Introduction. Banking project investments as one of the important factors of economic development of any country should be investigated. Despite the relative novelty of project financing in Ukraine, it should be studied because this process has been widely used for a long period in the world, which is confirmed by the implementation of large-scale projects. The purpose of the article is the implementation of a comparative analysis of bank project financing in Ukraine and the world during the pre-crisis macroeconomic situation, as well as the development of appropriate proposals for further development of the mentioned investments. Results. It substantiated the promotion project financing would be impossible in the conditions of the predominance of the short-term loans and the unwillingness of banking institutions to participate in the financing of infrastructure and investment projects. The factors hindering the development of bank project financing in Ukraine were determined. Significant potential for the rapid development of project financing has been fully confirmed by the corresponding market demand. However, for the development of project financing in Ukraine it is necessary to realistically and in detail assess the viability and profitability of projects. Also, the most effective leverage can and should be appropriate changes in the legislation that might give the guarantee bilateral protection of investors and creditors, as well as describe the approaches to the formation of a simplified mechanism for this type of financing.

scholarly journals The role of vaccination in global health

2014 ◽  
Vol 83 (2) ◽  
pp. 17-19
Author(s):  
Keegan Guidolin ◽  
Gaby Meglei
Keyword(s):  
Infectious Disease ◽  
Global Health ◽  
Infectious Diseases ◽  
Financial Support ◽  
Action Plan ◽  
Preventable Death ◽  
Eradication Program ◽  
The World ◽  
The Future

Perhaps the greatest medical advancement in history was the development of the vaccine. While previously helpless to stop the spread of deadly infectious diseases, humanity has since harnessed the power of vaccination and decreased the incidence of infectious disease across the globe. Smallpox was the first disease to be eradicated—the greatest of our triumphs. This success was due to the global coordinated effort of the Intensified Smallpox Eradication Program. Presently, measles is the top cause of vaccine-preventable death in the world, and despite enormous vaccination efforts, eradication is still distant. This is due in part to the antivaccination movement, which was fueled by an article by Wakefield, published in the Lancet in 1998. The future of vaccination is an ambitious and unclear one—with tremendous financial support and involvement, the Global Vaccine Action Plan (GVAP) aims to eliminate vaccine preventable illness within what they call the “Decade of Vaccines”.

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