scholarly journals Lack of HPV in pterygium with no evidence of autoinoculation and the role of cytokines in pterygium with dry eye

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lita Uthaithammarat ◽  
Ngamjit Kasetsuwan ◽  
Yuda Chongpison ◽  
Pimpetch Kasetsuwan ◽  
Usanee Reinprayoon ◽  
...  
Keyword(s):  
Dry Eye ◽  
Hpv Infection ◽  
Cross Sectional Study ◽  
Urine Samples ◽  
Vascular Endothelial ◽  
Cross Sectional ◽  
Hpv Dna ◽  
Polymerase Chain ◽  
Endothelial Growth Factor

AbstractThis study evaluated human papillomavirus’s (HPV) role in pterygium pathogenesis, its autoinoculation from genitalia to ocular surface, potential cytokines involved, and crosstalk cytokines between pterygium and dry eye (DE). This cross-sectional study enrolled 25 healthy controls (HCs) and 116 pterygium patients. Four subgroups of pterygium and DE were used in cytokine evaluations. Conjunctival and pterygium swabs and first-void urine samples (i.e., genitalia samples) were collected for HPV DNA detection using real-time polymerase chain reaction. Tear cytokines interleukin (IL)-6, IL-18, and vascular endothelial growth factor (VEGF) in tears were evaluated. No HPV DNA was detected in conjunctival or pterygium swabs. No association was found between HPV DNA in urine samples and that from conjunctival or pterygium swabs. Tear VEGF levels were significantly higher in pterygium patients than in HCs, with no markedly different levels between primary and recurrent pterygia. Tear IL-6, IL-18, and tear VEGF were significantly higher in participants with DE, regardless of pterygium status. In conclusion, HPV infection was not a pathogenic factor of pterygia. The hypothesis of HPV transmitting from the genitals to ocular surfaces was nullified. Tear VEGF was involved in both pterygia and DE, whereas tear IL-6 and IL-18 played roles only in DE.

scholarly journals The characteristics and risk factors of human papillomavirus infection: an outpatient population-based study in Changsha, Hunan

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bingsi Gao ◽  
Yu-Ligh Liou ◽  
Yang Yu ◽  
Lingxiao Zou ◽  
Waixing Li ◽  
...  
Keyword(s):  
Risk Factors ◽  
Protective Factor ◽  
Hpv Infection ◽  
Population Based ◽  
Cross Sectional Study ◽  
Categorical Variables ◽  
Chi Square ◽  
Cross Sectional ◽  
Hpv Dna ◽  
Test Kit

AbstractThis cross-sectional study investigated the characteristics of cervical HPV infection in Changsha area and explored the influence of Candida vaginitis on this infection. From 11 August 2017 to 11 September 2018, 12,628 outpatient participants ranged from 19 to 84 years old were enrolled and analyzed. HPV DNA was amplified and tested by HPV GenoArray Test Kit. The vaginal ecology was detected by microscopic and biochemistry examinations. The diagnosis of Candida vaginitis was based on microscopic examination (spores, and/or hypha) and biochemical testing (galactosidase) for vaginal discharge by experts. Statistical analyses were performed using SAS 9.4. Continuous and categorical variables were analyzed by t-tests and by Chi-square tests, respectively. HPV infection risk factors were analyzed using multivariate logistic regression. Of the total number of participants, 1753 were infected with HPV (13.88%). Females aged ≥ 40 to < 50 years constituted the largest population of HPV-infected females (31.26%). The top 5 HPV subtypes affecting this population of 1753 infected females were the following: HPV-52 (28.01%), HPV-58 (14.83%), CP8304 (11.47%), HPV-53 (10.84%), and HPV-39 (9.64%). Age (OR 1.01; 95% CI 1–1.01; P < 0.05) and alcohol consumption (OR 1.30; 95% CI 1.09–1.56; P < 0.01) were found to be risk factors for HPV infection. However, the presence of Candida in the vaginal flora was found to be a protective factor against HPV infection (OR 0.62; 95% CI 0.48–0.8; P < 0.001). Comparing with our previous study of 2016, we conclude that the subtype distribution of HPV infection is relatively constant in Changsha. Our data suggest a negative correlation between vaginal Candida and HPV, however, more radical HPV management is required in this area for perimenopausal women and those who regularly consume alcohol.

scholarly journals Imaging of COVID-19: Experience in BIRDEM General Hospital

2020 ◽  
pp. 56-62
Author(s):  
MA Taher ◽  
Jafreen Sultana ◽  
Ishtiaque Mohammad Behnom ◽  
Mohua Saha
Keyword(s):  
Global Health ◽  
General Hospital ◽  
Lower Lobe ◽  
Ground Glass Opacity ◽  
Cross Sectional Study ◽  
High Resolution Computed Tomography ◽  
Cross Sectional ◽  
Progression Of Disease ◽  
Polymerase Chain

Background: Pneumonia associated with the coronavirus disease 2019 (COVID-19) emerged in Wuhan of China which was recognized as a global health emergency. Role of imaging, specially high resolution computed tomography (HRCT) of chest is considered as an important tool in the early diagnosis, evaluation of disease progression and prompt management of COVID-19 patient in this global health pandemic. This study was designed to report the spectrum of HRCT imaging findings in COVID-19 infected patients. Methods: This cross-sectional study included 30 consecutive reverse transcriptase polymerase chain reaction (RT-PCR) positive COVID-19 patients who underwent HRCT chest from 1st of September 2020 to 30th October 2020 in BIRDEM General Hospital. Dhaka, Bangladesh. Prevalence, distribution, extent and type of abnormal lung findings were recorded. Results: Total 30 patients diagnosed with COVID-19 were included. Among them, 26 (86.6%) patients had fever and 17 (66.66%) patients had fatigability. The most frequent CT abnormality was ground glass opacity in (26, 86.67%). Crazy-paving pattern was found in 19 (63.33%) cases. Most patients had multiple lesions and involved all the 5 lobes The lesions were mostly peripheral (26, 86.6%) in distribution. Most commonly involved lobe is right lower lobe (27, 90%). Conclusion: Radiology & Imaging, specially HRCT of chest is an excellent modality in diagnosis of disease, evaluation of progression of disease, evaluation of post COVID-19 patient and prompt management of COVID- 19 patient in this global health emergency. Birdem Med J 2020; 10, COVID Supplement: 56-62

scholarly journals Human papillomavirus infection and risk factors to cervical cancer in asymptomatic women in the region of the Northeast of Brazil

2021 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Karol Fireman de Farias ◽  
Adrielle Sonara Gomes Silva ◽  
Ana Caroline Melo dos Santos ◽  
Adriely Ferreira da Silva ◽  
Denise Macedo da Silva ◽  
...  
Keyword(s):  
Risk Factors ◽  
Cervical Cancer ◽  
Oral Contraceptives ◽  
Hpv Infection ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Hpv Dna ◽  
Papillomavirus Infection ◽  
Behavioral Variables ◽  
Asymptomatic Women

Objective: This study sought to identify the presence of HPV infection and the risk factors related to susceptibility to cervical cancer in asymptomatic women. Methods: It is a cross-sectional study with 428 users attended Basic Health Units, in Arapiraca, Alagoas, Brazil. Sociodemographic, behavioral variables, and cytopathological reports were collected. Molecular detection of the HPV virus was performed by Nested-PCR. Statistical analysis was conducted with SPSS version 22.0. Results: A total of 428 women were studied, HPV DNA detected in 39.2% (n = 168), with a mean age of 41 years old. There was an association of HPV with use of oral contraceptives (p <0.016) and alcoholism (p <0.038). It was showed a higher frequency of positive HPV in women older than 25 years old (88.7%), up to 5 sexual partners (93.4%), up to 3 pregnancies (71.4%), and with the cytopathologic results within the limits of normality (61.9%). HPV was identified in 40.3% (104/258) of the women with results within the limits of normality. Conclusion: Our results suggest that the use of oral contraceptives and alcoholism may be considered as possible risk factors related to cervical oncogenesis. With this, it is necessary to propose interventions aimed at the health education of this population, actions of prevention, and early detection.

scholarly journals CORRELATION OF (PRO)RENIN RECEPTOR AND VASCULAR ENDOTHELIAL GROWTH FACTOR EXPRESSION LEVEL IN THIRD TRIMESTER OF PREECLAMPSIA PLACENTAS

2018 ◽  
Vol 11 (12) ◽  
pp. 308
Author(s):  
Nelly Marissa ◽  
Sri Widia A Jusman ◽  
Yuditiya Purwosunu ◽  
Ani Retno Prijanti
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Sandwich Elisa ◽  
Normal Pregnancy ◽  
Cross Sectional Study ◽  
Vascular Endothelial ◽  
Vegf Mrna ◽  
Cross Sectional ◽  
Relative Expression ◽  
Endothelial Growth Factor

Objective: This cross-sectional study was designed to correlate the expression of mRNA and protein of (pro)renin receptor ([P]RR) and vascular endothelial growth factor (VEGF) gene in preeclampsia placentas.Methods: We used 34 placenta tissues of normal pregnancy and 34 preeclampsia placenta tissues. Real-time polymerase chain reaction was used to measure relative expression of VEGF and (P)RR mRNAs, and protein concentration was measured using Sandwich ELISA technique.Results: VEGF mRNA relative expression of preeclampsia placenta was 2.83 times higher than the normal placenta (p=0.02). (P)RR mRNA relative expression on preeclampsia placenta was 1.7 times higher than the normal one (p=0.039). Expression of protein (P)RR and VEGF was lower on preeclampsia shown by p<0.05, R=0.441. From this research, it was considered that low protein of (P)RR and VEGF contributes to preeclampsia.Conclusion: There was a correlation between (P)RR and VEGF. It considers that there is any involvement of (P)RR on angiogenesis through VEGF expression in preeclampsia placenta.

scholarly journals High Prevalence of Human Papillomavirus Infection among Brazilian Pregnant Women with and without Human Immunodeficiency Virus Type 1

2009 ◽  
Vol 2009 ◽  
pp. 1-4 ◽  
Author(s):  
Emilia Moreira Jalil ◽  
Geraldo Duarte ◽  
Patrícia El Beitune ◽  
Renata Toscano Simões ◽  
Patrícia Pereira dos Santos Melli ◽  
...  
Keyword(s):  
Viral Load ◽  
Pregnant Women ◽  
Prevalence Ratio ◽  
Hpv Infection ◽  
Cross Sectional Study ◽  
Hiv Positive ◽  
Hiv Viral Load ◽  
Cross Sectional ◽  
Hpv Dna ◽  
Hiv Negative

Objective. To estimate HPV prevalence among pregnant women from Ribeirão Preto, Brazil, and the possible influence of HIV-1 infection on this prevalence.Methods. A cross-sectional study with 44 HIV-positive and 53 HIV-negative pregnant women was conducted. Cervicovaginal specimens were obtained from all women during gynecologic exam. HPV DNA, low and high risk HPV types, was detected using conventional PCR. Statistical analysis used Student'st-test, Mann-Whitney test, Fischer's Exact test, and prevalence ratios with 95% confidence interval.Results. HIV-positive pregnant women had higher proportion of HPV infection than HIV-negative pregnant women (79.5% versus 58.5%;P<.05). HPV positivity prevalence ratio for HIV-positive women was 1.36 (95% CI 1.04–1.8;P=.03). There was significant association between HIV viral load levels and HPV positivity (P<.05).Conclusions. Our results demonstrate higher HPV positivity in HIV-infected pregnant women. Higher values of HIV viral load were associated with HPV positivity.

scholarly journals Level of VEGF-A and interleukin 6 in lacrimal fluid of patients with diabetic retinopathy

2020 ◽  
Vol 14 (3) ◽  
pp. 664-673
Author(s):  
Christelle Domngang ◽  
Armelle Lowe Noutchie ◽  
Pascal Djamen Chuisseu ◽  
Freddy Nemg Simo ◽  
Borris Tietche Galani ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Interleukin 6 ◽  
Microvascular Complications ◽  
Cross Sectional Study ◽  
Elisa Test ◽  
Diabetic Patients ◽  
Vascular Endothelial ◽  
Cross Sectional ◽  
The Mean ◽  
Endothelial Growth Factor

Diabetic retinopathy (DR) is an ocular manifestation characterized by microvascular complications. The aim of this study was to investigate the relationship between diabetic retinopathy (DR) and two lacrimal angiogenic cytokines, interleukin-6 (Il-6) and vascular endothelial growth factor-A (VEGF-A). A cross-sectional study was conducted at Yaoundé Central Hospital from February,1 to June, 15, 2019. Twenty-six diabetic patients with retinopathy and 55 diabetics without retinopathy were enrolled. Tears were collected with Schirmer strips. The concentrations of VEGF-A and Il-6 were determined by the ELISA test. Statistical analysis was performed using Epi Data version 7.1.5.2. and GraphPad 5.0. The mean VEGF-A concentration was 163.4 ± 45.1 and 149± 81.8 pg/ml in patients with and without DR respectively (p= 0.292) while tear Il-6 level was 118.5±103 and 119±79.43 pg/ml in patients with and without DR respectively (p= 0.497). The mean tear VEGF level was 165.2 ± 76.10 and 153.50 ± 57.94 pg/mL in patients with and without diabetic maculopathy (DM) respectively (p=0.343) while tear Il-6 level was significantly lower in DM (54.92±32.68) compared to patients without DM (159.10±89.78) (p=0.047). Tear Il-6 level was significantly low in patients with DM. There was no association between the 2 lacrimal biomarkers and diabetic retinopathy. Further analysis is needed for this type of screening in diabetic patients.Keywords: eye, retina, tear, diabetes, biomarker; cytokine.

scholarly journals Relationship Between Vascular Endothelial Growth Factor Expression and Gleason Score in Prostate Carcinoma

2021 ◽  
Vol 2 (1) ◽  
pp. 82-88
Author(s):  
Fadillah ◽  
Heni Maulani ◽  
Nursanti Apriyani
Keyword(s):  
Gleason Score ◽  
Prostate Carcinoma ◽  
Significant Relationship ◽  
Prostate Adenocarcinoma ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Cross Sectional ◽  
Pathology Section ◽  
Endothelial Growth Factor

Introduction. Prostate cancer is the most common cancer in men worldwide and ranks second in the Laboratory of Anatomical Pathology, Dr. Mohammad Hoesin with the highest incidence, especially at the age of more than 60 years. Factors that play a role as a prognostic factor and therapy in prostate carcinoma, including VEGF. The role of VEGF expression in prostate carcinoma as a prognostic and histopathological factor which is an important predictor for the progression of prostate carcinoma. This study aims to determine the relationship between VEGF expression and Gleason score in prostate carcinoma. Method. This study was a cross-sectional observational study. Thirty samples diagnosed with prostate adenocarcinoma were derived from the results of the tranurethral resection of the prostate (TRUP) and prostatectomy. Samples were taken from the archives in the Anatomic Pathology section of Dr. Mohammad Hoesin Palembang (period January 1, 2011 to December 31, 2013. Then the sample was stained with VEGF antibody, identified and analyzed the VEGF relationship with the Gleason.z score. Results. The positivity of VEGF expression in prostate adenocarcinoma tended to be more prevalent in the Gleason score group ≥ 7 (43.3%) than in the Gleason score group <(10%). There was no significant relationship between VEGF expression and high Gleason score (p> 0.05). Conclusion. There was no significant relationship between VEGF expression and Gleason score in prostate adenocarcinoma

scholarly journals Cervical Dysplasia and High-Risk Human Papillomavirus Infections among HIV-Infected and HIV-Uninfected Adolescent Females in South Africa

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
David H. Adler ◽  
Melissa Wallace ◽  
Thola Bennie ◽  
Megan Mrubata ◽  
Beau Abar ◽  
...  
Keyword(s):  
High Risk ◽  
Pap Smear ◽  
Cervical Dysplasia ◽  
Pap Test ◽  
Hpv Infection ◽  
Cross Sectional Study ◽  
Cervical Lesions ◽  
Cross Sectional ◽  
Hpv Dna ◽  
Increased Risk

Background.HIV-infected adolescents may be at higher risk for high-grade cervical lesions than HIV-uninfected adolescents. The purpose of this study was to compare the prevalence of high-risk HPV (HR-HPV) infections and Pap smear abnormalities between these two groups.Methods.In this cross-sectional study, we compared the HPV DNA and Pap smear results between 35 HIV-infected and 50 HIV-uninfected adolescents in order to determine the prevalence of HR-HPV genotypes and cervical cytological abnormalities. Comparisons were made using Pearsonχ2and independent-samplest-tests analyses, and associations between demographic and behavioral characteristics and HPV infections were examined.Results.HIV-infected participants were more likely to be infected with any HPV (88.6% versus 48.0%;P<0.001) and with at least one HR-HPV (60.0% versus 24.0%;P=0.001), and to have multiple concurrent HPV infections (68.6% versus 22.0%;P<0.001). HPV 16 and 18 were relatively underrepresented among HR-HPV infections. Abnormal Pap test results were more common among HIV-infected participants (28.8% versus 12.0%;P=0.054). A history of smoking was associated with HR-HPV infection.Conclusions.HIV-infected adolescents have an increased risk of infection with HR-HPV and of Pap test abnormalities. The majority of HR-HPV infections among our participants would not be prevented by the currently available vaccinations against HPV.

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