Enantioseparation by crystallization using magnetic substrates

The effects of hypoglycin A on the metabolism of L-leucine-C14, L-alanine-C14, and L-glutamic-acid-C14 by rat liver slices have been investigated. Hypoglycin exerted markedly inhibitory effects on the conversion of leucine-C14 to fatty acid, cholesterol, and CO2. Conversion of alanine-C14 and glutamic acid-C14 to fatty acids was also inhibited by hypoglycin. No effects of hypoglycin on the conversion of C14-amino acids into protein or glycogen were demonstrated.

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Utilization in vivo of glucose and volatile fatty acids by sheep brain for the synthesis of acidic amino acids

Biochemical Journal ◽

10.1042/bj1010591 ◽

1966 ◽

Vol 101 (3) ◽

pp. 591-597 ◽

Cited By ~ 34

Author(s):

R M O'Neal ◽

R E Koeppe ◽

E I Williams

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Glutamic Acid ◽

Aspartic Acid ◽

Free Amino Acids ◽

Free Amino ◽

Specific Activity ◽

Tricarboxylic Acid Cycle ◽

Sheep Brain ◽

The Brain

1. Free glutamic acid, aspartic acid, glutamic acid from glutamine and, in some instances, the glutamic acid from glutathione and the aspartic acid from N-acetyl-aspartic acid were isolated from the brains of sheep and assayed for radioactivity after intravenous injection of [2-(14)C]glucose, [1-(14)C]acetate, [1-(14)C]butyrate or [2-(14)C]propionate. These brain components were also isolated and analysed from rats that had been given [2-(14)C]propionate. The results indicate that, as in rat brain, glucose is by far the best precursor of the free amino acids of sheep brain. 2. Degradation of the glutamate of brain yielded labelling patterns consistent with the proposal that the major route of pyruvate metabolism in brain is via acetyl-CoA, and that the short-chain fatty acids enter the brain without prior metabolism by other tissue and are metabolized in brain via the tricarboxylic acid cycle. 3. When labelled glucose was used as a precursor, glutamate always had a higher specific activity than glutamine; when labelled fatty acids were used, the reverse was true. These findings add support and complexity to the concept of the metabolic; compartmentation' of the free amino acids of brain. 4. The results from experiments with labelled propionate strongly suggest that brain metabolizes propionate via succinate and that this metabolic route may be a limited but important source of dicarboxylic acids in the brain.

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Antigenicity of polypeptides (poly-α-amino acids)—XVIII. Quantitative relationships among polymers containing glutamic acid, lysine and/or alannine rabbit antisera

Immunochemistry ◽

10.1016/0019-2791(66)90178-9 ◽

1966 ◽

Vol 3 (5) ◽

pp. 403-417 ◽

Cited By ~ 3

Author(s):

Paul H Maurer ◽

Paul Pinchuck ◽

Bernard F Gerulat

Keyword(s):

Amino Acids ◽

Glutamic Acid

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The uptake of amino acids by mouse cells (strain LS) during growth in batch culture and chemostat culture: the influence of cell growth rate

Proceedings of the Royal Society of London Series B Biological Sciences ◽

10.1098/rspb.1967.0073 ◽

1967 ◽

Vol 168 (1013) ◽

pp. 421-438 ◽

Cited By ~ 46

Keyword(s):

Amino Acids ◽

Growth Rate ◽

Amino Acid ◽

Cell Growth ◽

Glutamic Acid ◽

Batch Culture ◽

Amino Acid Uptake ◽

Essential Amino Acids ◽

Growth Yields ◽

Acid Uptake

The uptake of thirteen essential amino acids by mouse LS cells in suspension culture was determined by bacteriological assay methods. Chemostat continuous-flow cultures were used to determine the effect of different cell growth rates on the quantitative amino acid requirements for growth. The growth yields of the cells ( Y = g cell dry weight produced/g amino acid utilized) were calculated for each of the essential amino acids. A mixture of the non-essential amino acids, serine, alanine and glycine increased the cell yield from the essential amino acids. The growth yields from nearly all the essential amino acids in batch culture were increased when glutamic acid was substituted for the glutamine in the medium. The growth yields from the amino acids in batch culture were much less at the beginning than at the end of the culture. The highest efficiencies of conversion of amino acids to cell material were obtained by chemostat culture. When glutamic acid largely replaced the glutamine in the medium the conversion of amino acid nitrogen to cell nitrogen was 100 % efficient (that is, the theoretical yield was obtained) at the optimum growth rate (cell doubling time, 43 h). The maximum population density a given amino acid mixture will support can be calculated from the data. It is concluded that in several routinely used tissue culture media the cell growth is limited by the amino acid supply. In batch culture glutamine was wasted by (1) its spontaneous decomposition to pyrrolidone carboxylic acid and ammonia, and (2) its enzymic breakdown to glutamic acid and ammonia, but also glutamine was used less efficiently than glutamic acid. Study of the influence of cell growth rate on amino acid uptake rates per unit mass of cells indicated that a marked change in amino acid metabolism occurred at a specific growth rate of 0.4 day -1 (cell doubling time, 43 h). With decrease in specific growth rate below 0.4 day -1 there was a marked stimulation of amino acid uptake rate per cell and essential amino acids were consumed increasingly for functions other than synthesis of cell material.

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ChemInform Abstract: Amino Acids. Part 8. A Novel Synthesis of γ-Carboxy-L-glutamic Acid from L-5-Oxoproline Esters.

ChemInform ◽

10.1002/chin.199040307 ◽

1990 ◽

Vol 21 (40) ◽

Author(s):

F. EFFENBERGER ◽

W. MUELLER ◽

R. KELLER ◽

W. WILD ◽

T. ZIEGLER

Keyword(s):

Amino Acids ◽

Glutamic Acid ◽

Novel Synthesis

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Amino Acid Composition of Milk from Cow, Sheep and Goat Raised in Ailano and Valle Agricola, Two Localities of ‘Alto Casertano’ (Campania Region)

Foods ◽

10.3390/foods10102431 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2431

Author(s):

Nicola Landi ◽

Sara Ragucci ◽

Antimo Di Maro

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Glutamic Acid ◽

Protein Content ◽

Total Protein ◽

Free Amino ◽

Raw Milk ◽

Total Amino Acid ◽

Total Protein Content ◽

Campania Region

Cow, sheep and goat raw milk raised in Ailano and Valle Agricola territories (‘Alto Casertano’, Italy) were characterized (raw proteins, free and total amino acids content) to assess milk quality. Raw milk with the highest total protein content is sheep milk followed by goat and cow milk from both localities. Total amino acid content in cow, goat and sheep raw milk is 4.58, 4.81 and 6.62 g per 100 g, respectively, in which the most abundant amino acid is glutamic acid (~20.36 g per 100 g of proteins). Vice versa, the free amino acids content characteristic profiles are different for each species. In particular, the most abundant free amino acid in cow, sheep and goat raw milk is glutamic acid (9.07 mg per 100 g), tyrosine (4.72 mg per 100 g) and glycine (4.54 mg per 100 g), respectively. In addition, goat raw milk is a source of taurine (14.92 mg per 100 g), retrieved in low amount in cow (1.38 mg per 100 g) and sheep (2.10 mg per 100 g) raw milk. Overall, raw milk from ‘Alto Casertano’ show a high total protein content and are a good source of essential amino acids.

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Assessment of L-Citrulline, L-Arginine and L-Glutamic Acid Content in Selected Fruits, Vegetables, Seeds, and Nuts Sold in Markets in Nairobi City County, Kenya

European Journal of Agriculture and Food Sciences ◽

10.24018/ejfood.2020.2.5.100 ◽

2020 ◽

Vol 2 (5) ◽

Author(s):

Peninnah Mueni Mulwa ◽

Wilson Njue ◽

Margaret Ng’ang’a

Keyword(s):

Amino Acids ◽

Glutamic Acid ◽

Human Body ◽

Fruits And Vegetables ◽

Brain Health ◽

Button Mushroom ◽

The Central Nervous System ◽

Healing Of Wounds ◽

Statistical Analysis Software ◽

Internal Production

Background: L-citrulline, L-arginine, and L-glutamic acid are amino acids which are vital in the human body. L-citrulline boosts immunity, combats sarcopenia, detoxifies the liver, and enhances male fertility. L-arginine boosts internal production of nitric oxide, prevents abnormal blood clotting and accelerates healing of wounds. L-glutamic acid cleanses the central nervous system and improves overall brain health. Deficiency of these amino acids can lead to accumulation of ammonia and impact negatively to the nervous systems of the human body. This study aimed to identify and determine the levels of L-citrulline, L-arginine, and L-glutamic acid in selected fruits, vegetables, nuts, and seeds sold in markets in Nairobi City County, Kenya using Liquid Chromatography- Mass Spectrometry (LC-MS). Materials and Methods: Data was collected from 28 selected samples and data analysis was done using Statistical Analysis Software (SAS) version 9.4. Results: LC-MS showed the presence of both L-arginine and L-citrulline in most fruits, vegetables, and nuts while L-glutamic acid was present in the seeds. The levels of the amino acids in the fruits and vegetables were in the following range: L-citrulline (0.65- 19.41 mg/100g) in the button mushroom, cucumber, pumpkin, amaranthus, and kales, (3.16-3.79 mg/100g) in the watermelons and (1.57-10.21 mg/100g) in the nuts. L-arginine was in the range; 1.73 - 16.48 mg/100g in the amaranthus, kales, button mushroom, butternut squash, and cucumber, 5.44-6.56 mg/100g in the watermelons and 0.93-10.73 mg/100g in the nuts and L-glutamic acid (0.013-0.28 mg/100g) in the seeds of pumpkin, butternut, and watermelons. Conclusion: The results showed that locally available vegetables, fruits and nuts are rich in L-citrulline and L-arginine.

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Effect of Different Edaphic Crop Conditions on the Free Amino Acid Profile of PH-16 Dry Cacao Beans

Agronomy ◽

10.3390/agronomy11081637 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1637

Author(s):

Quintino Reis de Araujo ◽

Guilherme Amorim Homem de Abreu Loureiro ◽

Cid Edson Mendonça Póvoas ◽

Douglas Steinmacher ◽

Stephane Sacramento de Almeida ◽

...

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Glutamic Acid ◽

Free Amino Acid ◽

Free Amino Acids ◽

Free Amino ◽

Principal Component ◽

Amino Acid Profile ◽

Gamma Aminobutyric Acid ◽

Significant Difference

Free amino acids in cacao beans are important precursors to the aroma and flavor of chocolate. In this research, we used inferential and explanatory statistical techniques to verify the effect of different edaphic crop conditions on the free amino acid profile of PH-16 dry cacao beans. The decreasing order of free amino acids in PH-16 dry cacao beans is leucine, phenylalanine, glutamic acid, alanine, asparagine, tyrosine, gamma-aminobutyric acid, valine, isoleucine, glutamine, lysine, aspartic acid, serine, tryptophan, threonine, glycine. With the exception of lysine, no other free amino acid showed a significant difference between means of different edaphic conditions under the ANOVA F-test. The hydrophobic free amino acids provided the largest contribution to the explained variance with 58.01% of the first dimension of the principal component analysis. Glutamic acid stands out in the second dimension with 13.09%. Due to the stability of the biochemical profile of free amino acids in this clonal variety, it is recommended that cacao producers consider the genotype as the primary source of variation in the quality of cacao beans and ultimately the chocolate to be produced.

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