Streptomyces
          dd-carboxypeptidase as transpeptidases. The specificity for amino compounds acting as carboxyl acceptors

Harold R. Perkins; Manuel Nieto; Jean-Marie Frère; Mélina Leyh-Bouille; Jean-Marie Ghuysen

doi:10.1042/bj1310707

Streptomyces dd-carboxypeptidase as transpeptidases. The specificity for amino compounds acting as carboxyl acceptors

Biochemical Journal ◽

10.1042/bj1310707 ◽

1973 ◽

Vol 131 (4) ◽

pp. 707-718 ◽

Cited By ~ 40

Author(s):

Harold R. Perkins ◽

Manuel Nieto ◽

Jean-Marie Frère ◽

Mélina Leyh-Bouille ◽

Jean-Marie Ghuysen

Keyword(s):

Amino Acids ◽

Water Soluble ◽

Amino Compounds ◽

Wide Range

The ability of the water-soluble dd-carboxypeptidases of Streptomyces strains albus G, R61, K11 and R39 to perform transpeptidation was studied. The donor was diacetyl-l-lysyl-d-alanyl-d-alanine, and a whole range of amino acids, peptides and structurally related amino compounds were tested for acceptor function. No compound tested was an acceptor for the enzyme from strain albus G whereas the enzymes from strains R61 and K11 could utilize with varying efficiency a wide range of substances including peptides with N-terminal glycine or d-alanine, ω-amino acids, aminohexuronic acids, 6-aminopenicillanic acid and d-cycloserine. Certain peptides, when present in higher concentration, inhibited the transpeptidation observed at lower concentration. The enzyme from strain R39 would not use any dipeptide as an acceptor, but a few compounds that were not glycine or α-amino acids of the d-configuration did function thus. These were d-cycloserine and the lactams of meso- or racemic-diaminoadipic acid.

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High-temperature resistant water-soluble polymers derived from exotic amino acids

RSC Advances ◽

10.1039/d0ra06620f ◽

2020 ◽

Vol 10 (62) ◽

pp. 38069-38074

Author(s):

Sumant Dwivedi ◽

Aniruddha Nag ◽

Shigeki Sakamoto ◽

Yasuyoshi Funahashi ◽

Toyohiro Harimoto ◽

...

Keyword(s):

Amino Acids ◽

High Temperature ◽

High Performance ◽

Water Soluble ◽

Water Soluble Polymers ◽

Soluble Polymers ◽

Wide Range ◽

Soluble Polyimide ◽

Engineering Materials

High-performance water-soluble polymers have a wide range of applications from engineering materials to biomedical plastics. This article discusses the synthesis of water-soluble polyimide from bio-based monomers.

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Absolute Configuration of Mycosporine-Like Amino Acids, Their Wound Healing Properties and In Vitro Anti-Aging Effects

Marine Drugs ◽

10.3390/md18010035 ◽

2019 ◽

Vol 18 (1) ◽

pp. 35 ◽

Cited By ~ 2

Author(s):

Maria Orfanoudaki ◽

Anja Hartmann ◽

Mostafa Alilou ◽

Thomas Gelbrich ◽

Patricia Planchenault ◽

...

Keyword(s):

Amino Acids ◽

Wound Healing ◽

Absolute Configuration ◽

Water Soluble ◽

X Ray Diffraction ◽

Aging Effects ◽

Wide Range ◽

Glycation End Products ◽

Collagenase Inhibition

Mycosporine-like amino acids (MAAs) are water-soluble metabolites, reported to exhibit strong UV-absorbing properties. They have been found in a wide range of marine organisms, especially those that are exposed to extreme levels of sunlight, to protect them against solar radiation. In the present study, the absolute configuration of 14 mycosporine-like-amino acids was determined by combining the results of electronic circular dichroism (ECD) experiments and that of advanced Marfey’s method using LC-MS. The crystal structure of a shinorine hydrate was determined from single crystal X-ray diffraction data and its absolute configuration was established from anomalous-dispersion effects. Furthermore, the anti-aging and wound-healing properties of these metabolites were evaluated in three different assays namely the inhibition of collagenase, inhibition of advanced glycation end products (AGEs) and wound healing assay (scratch assay).

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Microsponges: An Overview

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v4i4.8860 ◽

2017 ◽

Vol 4 (4) ◽

Author(s):

Hamid Hussain ◽

Divya Juyal ◽

Archana Dhyani

Keyword(s):

Controlled Release ◽

Delivery System ◽

Oral Delivery ◽

Active Agent ◽

Topical Delivery ◽

Water Soluble ◽

Wide Range ◽

Porous Beads

Microsponge and Nanosponge delivery System was originally developed for topical delivery of drugs can also be used for controlled oral delivery of drugs using water soluble and bioerodible polymers. Microsponge delivery system (MDS) can entrap wide range of drugs and then release them onto the skin over a time by difussion mechanism to the skin. It is a unique technology for the controlled release of topical agents and consists of nano or micro porous beads loaded with active agent and also use for oral delivery of drugs using bioerodible polymers.

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The content of decenoic acids in drone brood preparations and combined preparations based on it

Biomics ◽

10.31301/2221-6197.bmcs.2020-29 ◽

2020 ◽

Vol 12 (3) ◽

pp. 389-393

Author(s):

D.V. Mitrofanov ◽

N.V. Budnikova

Keyword(s):

Royal Jelly ◽

Storage Conditions ◽

Water Soluble ◽

Biologically Active Substances ◽

Biologically Active ◽

Strict Adherence ◽

Drone Brood ◽

Wide Range ◽

Active Substances ◽

Melanin Complex

The drone brood contains a large number of substances with antioxidant activity. These substances require stabilization and strict adherence to storage conditions. Among these substances are unique decenoic acids, the content of which is an indicator of the quality of drone brood and products based on it. The ability of drone brood to reduce the manifestations of oxidative stress is shown. There are dietary supplements for food and drugs based on drone brood, which are used for a wide range of diseases. Together with drone brood, chitosan-containing products, propolis, royal jelly can be used. They enrich the composition with their own biologically active substances and affect the preservation of the biologically active substances of the drone brood. Promising are the products containing, in addition to the drone brood, a chitin-chitosan-melanin complex from bees, propolis, royal jelly. The chitin-chitosan-melanin complex in the amount of 5% in the composition of the adsorbent practically does not affect the preservation of decenic acids, while in the amount of 2% and 10% it somewhat worsens. The acid-soluble and water-soluble chitosan of marine crustaceans significantly worsens the preservation of decenoic acids in the product. Drone brood with royal jelly demonstrates a rather high content of decenoic acids. When propolis is introduced into the composition of the product, the content of decenoic acids increases according to the content of propolis.

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Autophagy Deficiency by Atg4B Loss Leads to Metabolomic Alterations in Mice

Metabolites ◽

10.3390/metabo11080481 ◽

2021 ◽

Vol 11 (8) ◽

pp. 481

Author(s):

Gemma G. Martínez-García ◽

Raúl F. Pérez ◽

Álvaro F. Fernández ◽

Sylvere Durand ◽

Guido Kroemer ◽

...

Keyword(s):

Skeletal Muscle ◽

Amino Acids ◽

Mammalian Cells ◽

Tissue Homeostasis ◽

In Vivo Studies ◽

Protective Mechanism ◽

Cardiac Damage ◽

Wide Range ◽

First Time

Autophagy is an essential protective mechanism that allows mammalian cells to cope with a variety of stressors and contributes to maintaining cellular and tissue homeostasis. Due to these crucial roles and also to the fact that autophagy malfunction has been described in a wide range of pathologies, an increasing number of in vivo studies involving animal models targeting autophagy genes have been developed. In mammals, total autophagy inactivation is lethal, and constitutive knockout models lacking effectors of this route are not viable, which has hindered so far the analysis of the consequences of a systemic autophagy decline. Here, we take advantage of atg4b−/− mice, an autophagy-deficient model with only partial disruption of the process, to assess the effects of systemic reduction of autophagy on the metabolome. We describe for the first time the metabolic footprint of systemic autophagy decline, showing that impaired autophagy results in highly tissue-dependent alterations that are more accentuated in the skeletal muscle and plasma. These changes, which include changes in the levels of amino-acids, lipids, or nucleosides, sometimes resemble those that are frequently described in conditions like aging, obesity, or cardiac damage. We also discuss different hypotheses on how impaired autophagy may affect the metabolism of several tissues in mammals.

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Interactions of water-soluble zinc porphyrin with amino acids

Inorganica Chimica Acta ◽

10.1016/s0020-1693(00)88867-2 ◽

1988 ◽

Vol 153 (4) ◽

pp. 199-200 ◽

Cited By ~ 9

Author(s):

E. Mikros ◽

A. Gaudemer ◽

R. Pasternack

Keyword(s):

Amino Acids ◽

Water Soluble ◽

Zinc Porphyrin ◽

Soluble Zinc

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Incorporation of alternative amino acids into cyanophycin by different cyanophycin synthetases heterologously expressed in Corynebacterium glutamicum

AMB Express ◽

10.1186/s13568-021-01217-5 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Ramona Wördemann ◽

Lars Wiefel ◽

Volker F. Wendisch ◽

Alexander Steinbüchel

Keyword(s):

Amino Acids ◽

Dry Weight ◽

Growth Conditions ◽

Amino Acid Biosynthesis ◽

Water Soluble ◽

Lysine Content ◽

Polyaspartic Acid ◽

Acid Biosynthesis ◽

Cyanophycin Synthetase ◽

Potential Precursor

AbstractCyanophycin (multi-l-arginyl-poly-l-aspartic acid; also known as cyanophycin grana peptide [CGP]) is a biopolymer that could be used in various fields, for example, as a potential precursor for the synthesis of polyaspartic acid or for the production of CGP-derived dipeptides. To extend the applications of this polymer, it is therefore of interest to synthesize CGP with different compositions. A recent re-evaluation of the CGP synthesis in C. glutamicum has shown that C. glutamicum is a potentially interesting microorganism for CGP synthesis with a high content of alternative amino acids. This study shows that the amount of alternative amino acids can be increased by using mutants of C. glutamicum with altered amino acid biosynthesis. With the DM1729 mutant, the lysine content in the polymer could be increased up to 33.5 mol%. Furthermore, an ornithine content of up to 12.6 mol% was achieved with ORN2(Pgdh4). How much water-soluble or insoluble CGP is synthesized is strongly related to the used cyanophycin synthetase. CphADh synthesizes soluble CGP exclusively. However, soluble CGP could also be isolated from cells expressing CphA6308Δ1 or CphA6308Δ1_C595S in addition to insoluble CGP in all examined strains. The point mutation in CphA6308Δ1_C595S partially resulted in a higher lysine content. In addition, the CGP content could be increased to 36% of the cell dry weight under optimizing growth conditions in C. glutamicum ATCC13032. All known alternative major amino acids for CGP synthesis (lysine, ornithine, citrulline, and glutamic acid) could be incorporated into CGP in C. glutamicum.

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Destruxin A Interacts with Aminoacyl tRNA Synthases in Bombyx mori

Journal of Fungi ◽

10.3390/jof7080593 ◽

2021 ◽

Vol 7 (8) ◽

pp. 593

Author(s):

Jingjing Wang ◽

Alexander Berestetskiy ◽

Qiongbo Hu

Keyword(s):

Amino Acids ◽

Protein Synthesis ◽

Bombyx Mori ◽

Metarhizium Anisopliae ◽

Free Amino ◽

Molecular Target ◽

Trna Synthetases ◽

Interaction Mode ◽

Aminoacyl Trna Synthetases ◽

Wide Range

Destruxin A (DA), a hexa-cyclodepsipeptidic mycotoxin produced by the entomopathogenic fungus Metarhizium anisopliae, exhibits insecticidal activities in a wide range of pests and is known as an innate immunity inhibitor. However, its mechanism of action requires further investigation. In this research, the interactions of DA with the six aminoacyl tRNA synthetases (ARSs) of Bombyx mori, BmAlaRS, BmCysRS, BmMetRS, BmValRS, BmIleRS, and BmGluProRS, were analyzed. The six ARSs were expressed and purified. The BLI (biolayer interferometry) results indicated that DA binds these ARSs with the affinity indices (KD) of 10−4 to 10−5 M. The molecular docking suggested a similar interaction mode of DA with ARSs, whereby DA settled into a pocket through hydrogen bonds with Asn, Arg, His, Lys, and Tyr of ARSs. Furthermore, DA treatments decreased the contents of soluble protein and free amino acids in Bm12 cells, which suggested that DA impedes protein synthesis. Lastly, the ARSs in Bm12 cells were all downregulated by DA stress. This study sheds light on exploring and answering the molecular target of DA against target insects.

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A Water-Soluble Copper-Immobilized Covalent Organic Framework Functioning as an “OFF–ON” Fluorescent Sensor for Amino Acids

Materials Advances ◽

10.1039/d1ma00234a ◽

2021 ◽

Author(s):

Lamiaa Reda Ahmed ◽

Ahmed F. M. EL-Mahdy ◽

Cheng-Tang Pan ◽

Shiao-Wei Kuo

Keyword(s):

Amino Acids ◽

Fluorescent Sensor ◽

Water Soluble ◽

One Pot ◽

Covalent Organic Framework ◽

The One ◽

Organic Framework

In this paper, we describe the construction of a new fluorescent hydroxyl- and hydrazone-based covalent organic framework (TFPB-DHTH COF) through the one-pot polycondensation of 1,3,5-tris(4-formylphenyl)benzene (TFPB) and 2,5-dihydroxyterephthalohydrazide (DHTH) under...

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