scholarly journals Absolute Configuration of Mycosporine-Like Amino Acids, Their Wound Healing Properties and In Vitro Anti-Aging Effects

Marine Drugs ◽  
2019 ◽  
Vol 18 (1) ◽  
pp. 35 ◽  
Author(s):  
Maria Orfanoudaki ◽  
Anja Hartmann ◽  
Mostafa Alilou ◽  
Thomas Gelbrich ◽  
Patricia Planchenault ◽  
...  
Keyword(s):  
Amino Acids ◽  
Wound Healing ◽  
Absolute Configuration ◽  
Water Soluble ◽  
X Ray Diffraction ◽  
Aging Effects ◽  
Wide Range ◽  
Glycation End Products ◽  
Collagenase Inhibition

Mycosporine-like amino acids (MAAs) are water-soluble metabolites, reported to exhibit strong UV-absorbing properties. They have been found in a wide range of marine organisms, especially those that are exposed to extreme levels of sunlight, to protect them against solar radiation. In the present study, the absolute configuration of 14 mycosporine-like-amino acids was determined by combining the results of electronic circular dichroism (ECD) experiments and that of advanced Marfey’s method using LC-MS. The crystal structure of a shinorine hydrate was determined from single crystal X-ray diffraction data and its absolute configuration was established from anomalous-dispersion effects. Furthermore, the anti-aging and wound-healing properties of these metabolites were evaluated in three different assays namely the inhibition of collagenase, inhibition of advanced glycation end products (AGEs) and wound healing assay (scratch assay).

scholarly journals Cellular effects of glycine and trehalose air-polishing powders on human gingival fibroblasts in vitro

2021 ◽  
Author(s):  
Jens Weusmann ◽  
James Deschner ◽  
Jean-Claude Imber ◽  
Anna Damanaki ◽  
Natalia D. P. Leguizamón ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Function ◽  
Nuclear Translocation ◽  
In Vitro Study ◽  
Human Gingival Fibroblasts ◽  
Mrna Levels ◽  
Gingival Fibroblasts ◽  
Air Polishing ◽  
Wide Range

Abstract Objectives Air-polishing has been used in the treatment of periodontitis and gingivitis for years. The introduction of low-abrasive powders has enabled the use of air-polishing devices for subgingival therapy. Within the last decade, a wide range of different low-abrasive powders for subgingival use has been established. In this study, the effects of a glycine powder and a trehalose powder on human gingival fibroblasts (HGF) were investigated. Methods HGF were derived from three systemically and periodontally healthy donors. After 24 h and 48 h of incubation time, mRNA levels, and after 48 h, protein levels of TNFα, IL-8, CCL2, and VEGF were determined. In addition, NF-κB p65 nuclear translocation and in vitro wound healing were assessed. Statistical analysis was performed by ANOVA and post hoc Dunnett’s and Tukey’s tests (p < 0.05). Results Glycine powder significantly increased the expression of proinflammatory genes and showed exploitation of the NF-κB pathway, albeit trehalose powder hardly interfered with cell function and did not trigger the NF-κB pathway. In contrast to trehalose, glycine showed a significant inhibitory effect on the in vitro wound healing rate. Conclusion Subgingivally applicable powders for air-polishing devices can regulate cell viability and proliferation as well as cytokine expression. Our in vitro study suggests that the above powders may influence HGF via direct cell effects. Trehalose appears to be relatively inert compared to glycine powder.

scholarly journals Solid-state studies and antioxidant properties of the γ-cyclodextrin·fisetin inclusion compound

2017 ◽  
Vol 13 ◽  
pp. 2138-2145 ◽  
Author(s):  
Joana M Pais ◽  
Maria João Barroca ◽  
Maria Paula M Marques ◽  
Filipe A Almeida Paz ◽  
Susana S Braga
Keyword(s):  
Inclusion Compound ◽  
Antioxidant Properties ◽  
Physicochemical Analysis ◽  
Water Soluble ◽  
Solid Inclusion ◽  
Human Consumption ◽  
X Ray Diffraction ◽  
Wide Range ◽  
Soluble Solid ◽  
Cp Mas Nmr

Fisetin is a natural antioxidant with a wide range of nutraceutical properties, including antidiabetic, neuroprotecting, and suppression or prevention of tumors. The present work describes the preparation of a water-soluble, solid inclusion compound of fisetin with gamma-cyclodextrin (γ-CD), a cyclic oligosaccharide approved for human consumption. A detailed physicochemical analysis of the product is carried out using elemental analysis, powder X-ray diffraction (PXRD), Raman, infrared and 13C{1H} CP-MAS NMR spectroscopies, and thermal analysis (TGA) to verify fisetin inclusion and to present a hypothetical structural arrangement for the host–guest units. The antioxidant activity of the γ-CD·fisetin inclusion compound is evaluated by the DPPH assay.

scholarly journals Amino Acids in Entomopathogenic Fungi Cultured In Vitro

Agronomy ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1899
Author(s):  
Lech Wojciech Szajdak ◽  
Stanisław Bałazy ◽  
Teresa Meysner
Keyword(s):  
Amino Acids ◽  
Entomopathogenic Fungi ◽  
Pathogenic Fungi ◽  
Individual Species ◽  
Conidiobolus Coronatus ◽  
Fungal Strains ◽  
Isaria Farinosa ◽  
Lecanicillium Lecanii ◽  
Wide Range

The content of bounded amino acids in six entomopathogenic fungi was identified and determined. Analyzing the elements characterizing the pathogenicity of individual species of fungi based on infectivity criteria, ranges of infected hosts, and the ability to induce epizootics, these can be ranked in the following order: Isaria farinosa, Isaria tenuipes, Isaria fumosorose, Lecanicillium lecanii, Conidiobolus coronatus, Isaria coleopterorum. These fungi represent two types of Hyphomycetales-Paecilomyces Bainier and Verticillium Nees ex Fr. and one type of Entomophtorales-Conidiobolus Brefeld. Our study indicates that there are significant quantitative and qualitative differences of bounded amino acids in the entomopathogenic fungal strains contained in the mycelium between high and low pathogenicity strains. The richest composition of bounded amino acids has been shown in the mycelium of the Isaria farinosa strain, which is one of the most commonly presented pathogenic fungi in this group with a very wide range of infected hosts and is the most frequently recorded in nature as an important factor limiting the population of insects.

scholarly journals In vitro assessment of the capacity of certain mycotoxin binders to adsorb some amino acids and water-soluble vitamins

2020 ◽  
Vol 103 (4) ◽  
pp. 3125-3132
Author(s):  
A. Kihal ◽  
M. Rodriguez-Prado ◽  
C. Godoy ◽  
C. Cristofol ◽  
S. Calsamiglia
Keyword(s):  
Amino Acids ◽  
Water Soluble ◽  
In Vitro Assessment

scholarly journals Effect of Oral Administration of Active Peptides of Pinctada Martensii on the Repair of Skin Wounds

Marine Drugs ◽  
2019 ◽  
Vol 17 (12) ◽  
pp. 697 ◽  
Author(s):  
Faming Yang ◽  
Xiaoming Qin ◽  
Ting Zhang ◽  
Chaohua Zhang ◽  
Haisheng Lin
Keyword(s):  
Amino Acids ◽  
Wound Healing ◽  
Clinical Problem ◽  
Skin Wound ◽  
Type Iii Collagen ◽  
Bioactive Substances ◽  
Skin Wound Healing ◽  
Active Peptides ◽  
Pinctada Martensii

Skin wound healing, especially chronic wound healing, is a common challenging clinical problem. It is urgent to broaden the sources of bioactive substances that can safely and efficiently promote skin wound healing. This study aimed to observe the effects of active peptides (APs) of the mantle of Pinctada martensii on wound healing. After physicochemical analysis of amino acids and mass spectrometry of APs, the effect of APs on promoting healing was studied through a whole cortex wound model on the back of mice for 18 consecutive days. The results showed that APs consisted of polypeptides with molecular weights in the range 302.17–2936.43 Da. The content of polypeptides containing 2–15 amino acids accounted for 73.87%, and the hydrophobic amino acids accounted for 56.51%. Results of in vitro experimentation showed that mice in APs-L group which were fed a low dose of APs (0.5 g/kg bw) had a shortened epithelialization time due to a shortening inflammatory period (p < 0.05). Mechanistically, this relied on its specific ability to promote the proliferation of CD31, FGF and EGF which accelerated the percentage of wound closure. Moreover, the APs-L group mice had enhanced collagen synthesis and increased type III collagen content in their wounds through a TGF-β/Smad signaling pathway (p > 0.05). Consequently, scar formation was inhibited and wound healing efficiency was significantly improved. These results show that the APs of Pinctada martensii promote dermal wound healing in mice and have tremendous potential for development and utilization in skin wound healing.

The metabolism of acetate by rumen microorganisms

1974 ◽  
Vol 20 (2) ◽  
pp. 183-185 ◽  
Author(s):  
B. Emmanuel ◽  
L. P. Milligan ◽  
B. V. Turner
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Nucleic Acids ◽  
Glutamic Acid ◽  
Acetate Metabolism ◽  
Rumen Microorganisms ◽  
Wide Range

Rumen contents were incubated in vitro with acetate-1-14C. Significant amounts of 14C were incorporated into rumen microbial proteins, nucleic acids, and lipids. Serine, glutamic acid, methionine, and cystine were highly labeled, whereas less, or insignificant radioactivity was found in other amino acids. Acetate was incorporated into a wide range of microbial fatty acids. The quantitative significance of acetate metabolism is discussed.

scholarly journals Streptomyces dd-carboxypeptidase as transpeptidases. The specificity for amino compounds acting as carboxyl acceptors

1973 ◽  
Vol 131 (4) ◽  
pp. 707-718 ◽  
Author(s):  
Harold R. Perkins ◽  
Manuel Nieto ◽  
Jean-Marie Frère ◽  
Mélina Leyh-Bouille ◽  
Jean-Marie Ghuysen
Keyword(s):  
Amino Acids ◽  
Water Soluble ◽  
Amino Compounds ◽  
Wide Range

The ability of the water-soluble dd-carboxypeptidases of Streptomyces strains albus G, R61, K11 and R39 to perform transpeptidation was studied. The donor was diacetyl-l-lysyl-d-alanyl-d-alanine, and a whole range of amino acids, peptides and structurally related amino compounds were tested for acceptor function. No compound tested was an acceptor for the enzyme from strain albus G whereas the enzymes from strains R61 and K11 could utilize with varying efficiency a wide range of substances including peptides with N-terminal glycine or d-alanine, ω-amino acids, aminohexuronic acids, 6-aminopenicillanic acid and d-cycloserine. Certain peptides, when present in higher concentration, inhibited the transpeptidation observed at lower concentration. The enzyme from strain R39 would not use any dipeptide as an acceptor, but a few compounds that were not glycine or α-amino acids of the d-configuration did function thus. These were d-cycloserine and the lactams of meso- or racemic-diaminoadipic acid.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY AND WOUND HEALING ACTIVITY OF WHEATGRASS BY 1,1- DIPHENYL, 2 PICRYLHYDRAZYL METHOD

2018 ◽  
Vol 11 (12) ◽  
pp. 170
Author(s):  
Veermaneni Alekhya ◽  
Thiyagarajan Deepan ◽  
Magharla Dasaratha Dhanaraju
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Skin Diseases ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Wheat Plant ◽  
Wide Range ◽  
Wound Healing Activity ◽  
In Vitro Antioxidant Activity

Objective: This study was designed to evaluate in vitro antioxidant activity and wound healing activity in Triticum aestivum (wheat grass).Methods: T. aestivum commonly known as Wheatgrass had a wide range of health benefits among the young grass of common wheat plant components includes chlorophyll, flavonoids, and Vitamins A, C, and E. Wheatgrass is used in Folklore medicine for treatment of skin diseases and wound healing. In our present study, petroleum ether, ethanol and aqueous extracts of T. aestivum have been evaluated for in vitro antioxidant activity and wound healing activity by 1,1- diphenyl, 2 Picrylhydrazyl radical scavenging activity, and Chick chorioallantoic method, respectively.Results: The results of both the assay showed that all the extracts of T. aestivum have significant antioxidant and wound healing activity on dose-dependent manner.Conclusion: The wheatgrass has antioxidant and wound healing activity.

scholarly journals Anti-inflammatory Anthraquinones from the Crinoid Himerometra magnipinna

2015 ◽  
Vol 10 (2) ◽  
pp. 1934578X1501000
Author(s):  
Yen-You Lin ◽  
Su-June Tsai ◽  
Michael Y. Chiang ◽  
Zhi-Hong Wen ◽  
Jui-Hsin Su
Keyword(s):  
Single Crystal ◽  
Diffraction Analysis ◽  
Absolute Configuration ◽  
Spectroscopic Data ◽  
Test Compound ◽  
X Ray Diffraction ◽  
X Ray ◽  
Anti Inflammatory ◽  
Raw264.7 Macrophage

Chemical investigation of a crinoid Himerometra magnipinna has afforded three anthraquinones (1–3), including one new metabolite, (+)-rhodoptilometrin (1). The structures of these compounds were elucidated on the basis of their spectroscopic data and the absolute configuration of 1 was further confirmed by single-crystal X-ray diffraction analysis. In the in vitro anti-inflammatory effects test, compound 2 was found to significantly inhibit the accumulation of the pro-inflammatory iNOS protein of the LPS-stimulated RAW264.7 macrophage cells.

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