scholarly journals The supramolecular organization of ovomucin. Biophysical and morphological studies

1990 ◽  
Vol 266 (3) ◽  
pp. 697-706 ◽  
Author(s):  
C Rabouille ◽  
M A Aon ◽  
G Muller ◽  
J Cartaud ◽  
D Thomas
Keyword(s):  
Light Scattering ◽  
Spatial Organization ◽  
Unit Length ◽  
Random Coil ◽  
Radius Of Gyration ◽  
Supramolecular Organization ◽  
Linear Character ◽  
Morphological Studies ◽  
Exclusion Chromatography

Ovomucin participates in the ovomucin-gel-forming properties because of its shape and its ability to interact in a specific spatial organization. Purified from chicken egg-white by exclusion chromatography with Sephacryl S-300 and Sepharose CL-2B and analysed by light-scattering, it exhibited an Mr of about 40 x 10(6). This large Mr can be explained by the aggregation of polymers that can be degraded into 3 x 10(6)-Mr fragments by reduction with dithiothreitol. The values for hydrodynamic parameters such as Mr, radius of gyration, hydrodynamic radius, mass per unit length and combinations of them suggested that ovomucin is a linear and highly flexible molecule conferring upon it a random-coil-like structure in 0.2 M-NaCl solution. Analysis of the ovomucin molecules by electron microscopy revealed its linear character but also indicated a lower Mr than that obtained in the light-scattering experiments. By temperature-induced non-specific aggregation of an ovomucin solution containing other globular egg proteins, an attempt was made to find out what conditions are required for gel formation and to examine the quality of aggregation that is obtained under these conditions. Results show that the viscosity of the solution did not increase after heat treatment. Apparently, in the ovomucin gel, specific spatial organization of the ovomucin molecules is required for hydrogel formation.

LIGHT-SCATTERING STUDIES ON ALUMINUM DISTEARATE

1958 ◽  
Vol 36 (11) ◽  
pp. 1584-1595 ◽  
Author(s):  
A. E. Leger ◽  
J. C. Hyde ◽  
H. Sheffer
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Intrinsic Viscosity ◽  
Benzene Solution ◽  
Unit Length ◽  
Random Coil ◽  
Linear Molecule ◽  
Radius Of Gyration ◽  
Average Unit ◽  
Scattering Study

A light-scattering study of aluminum distearate in dilute benzene solution suggests that it is a linear molecule of random coil shape. This is further substantiated by intrinsic viscosity – molecular weight data, the constancy of the ratio of the molecular weight to the square of the radius of gyration (Rg), the value of Φ in Flory's intrinsic viscosity – molecular weight relationship, and the close agreement between the theoretical and experimental results found for the unit length of the polymer chain. This average unit length has been calculated to be 4.46 Å.

Polymer nanoparticle sizes from dynamic light scattering and size exclusion chromatography: the case study of polysilanes

Soft Matter ◽  
2018 ◽  
Vol 14 (23) ◽  
pp. 4735-4740 ◽  
Author(s):  
Artem Badasyan ◽  
Andraž Mavrič ◽  
Irena Kralj Cigić ◽  
Tim Bencik ◽  
Matjaz Valant
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Polymer Nanoparticle ◽  
Exclusion Chromatography ◽  
Nanoparticle Sizes

Dynamic light scattering (DLS) and size exclusion chromatography (SEC), that are among the most popular methods for determining polymer nanoparticle (PNP) sizes, essentially depend on the quality of solution.

scholarly journals Size and conformation of Ficoll as determined by size-exclusion chromatography followed by multiangle light scattering

2010 ◽  
Vol 298 (1) ◽  
pp. F205-F208 ◽  
Author(s):  
William H. Fissell ◽  
Christina L. Hofmann ◽  
Ross Smith ◽  
Michelle H. Chen
Keyword(s):  
Light Scattering ◽  
Size Exclusion Chromatography ◽  
Conformational Changes ◽  
Physiological Saline ◽  
Transport Processes ◽  
Size Exclusion ◽  
Random Coil ◽  
Filtration Barrier ◽  
Exclusion Chromatography

The characteristics of the glomerular filtration barrier (GFB) are challenging to measure, as macromolecular solutes in blood may be metabolized or transported by various cells in the kidney. Urinary solute concentrations generally reflect the cumulative influence of multiple transport processes rather than the intrinsic behavior of the GFB alone. Synthetic tracer molecules which are not secreted, absorbed, or modified by the kidney are useful tools. Ficoll, a globular polymer of epichlorohydrin and sucrose, is round, physiologically inert, and easily labeled, making it a nearly ideal glomerular probe. Fissell et al. reported filtration data suggesting that Ficoll was not as spherical as had been previously suggested (Fissell WH, Manley S, Dubnisheva A, Glass J, Magistrelli J, Eldridge AN, Fleischman AJ, Zydney AL, Roy S. Am J Physiol Renal Physiol 293: F1209–F1213, 2007). More recently, two investigators published comparisons of neutral and anionic Ficoll clearance that suggest Ficoll may undergo conformational changes when chemically derivatized (Asgeirsson D, Venturoli D, Rippe B, Rippe C. Am J Physiol Renal Physiol 291: F1083–F1089, 2006; Guimaraes MAM, Nikolovski J, Pratt LM, Greive K, Comper WD. Am J Physiol Renal Physiol 285: F1118–F1124, 2003). To investigate Ficoll's characteristics further, we examined two commercial preparations, Ficoll 70 and Ficoll 400, by size-exclusion chromatography using a differential refractive index detector combined with light-scattering and viscosity detectors. A slope of 0.45 was obtained from the plot of the logarithm of molecular mass against the logarithm of root-mean square radius. The Mark-Houwink exponent values of 0.34 and 0.36 were calculated for Ficoll 70 and Ficoll 400, respectively. These results suggest Ficoll's conformation in physiological saline solution is likely intermediate between a solid sphere and a well-solvated linear random coil. The measurements help explain our previous observations and guide interpretation of in vivo experiments.

scholarly journals MOLECULAR SIZE AND CONFIGURATION OF CELLULOSE TRINITRATE IN SOLUTION

1958 ◽  
Vol 36 (6) ◽  
pp. 952-969 ◽  
Author(s):  
M. M. Huque ◽  
D. A. I. Goring ◽  
S. G. Mason
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Ethyl Acetate ◽  
Average Molecular Weight ◽  
Molecular Size ◽  
Random Coil ◽  
Radius Of Gyration ◽  
Molecular Weight Range ◽  
Scattering Measurements ◽  
The Relationship

Viscosity and light-scattering measurements were made on several fractions and two unfractionated samples of cellulose trinitrate. The samples were prepared from bleached ramie, unbleached ramie, and cotton linters. The solvents were acetone and ethyl acetate. Viscosity was measured in a multishear viscometer designed for the purpose. Light-scattering measurements were made in a Brice-Phoenix Light-scattering Photometer modified to accommodate a cell which could be ultracentrifuged.The range of molecular weight investigated was from 6.5 × 105 to 25.0 × 105 The relationship between the z-average mean-square radius of gyration, [Formula: see text] and the z-average molecular weight was approximately linear in both solvents. The ratio of [Formula: see text] (where [Formula: see text] is the value of [Formula: see text] in the unperturbed state) was found constant in acetone but to increase with [Formula: see text] in ethyl acetate. This indicated that, whereas in acetone random coil configuration was attained, a configurational transition occurred in ethyl acetate in the molecular weight range investigated.The value of the exponent a in the relationship between intrinsic viscosity and molecular weight was found to be lower than unity but approximately equal in both solvents.The significance of the experimental data is discussed.

Small-Angle X-Ray and Light Scattering Studies on the Influence of Mg2+ Ions on the Structure of the RNA from Bacteriophage MS 2

1985 ◽  
Vol 40 (3-4) ◽  
pp. 234-241 ◽  
Author(s):  
Gertraud Ribitsch ◽  
Rita De Clercq ◽  
Waltraud Folkhard ◽  
Peter Zipper ◽  
Josef Schurz ◽  
...  
Keyword(s):  
Light Scattering ◽  
Secondary Structure ◽  
Low Temperature ◽  
Cross Section ◽  
Tertiary Structure ◽  
Unit Length ◽  
Radius Of Gyration ◽  
X Ray ◽  
X Ray Scattering ◽  
Ray Scattering

The influence of Mg2+ ions on the secondary and tertiary structure of the RNA from bacteriophage MS2 was investigated by small-angle X-ray scattering and light scattering and by sedimentation experiments. The analysis of the outer part of the X-ray scattering curve obtained at low temperature in the absence of Mg2+ yielded a cross-section radius of gyration of 0.88 nm and a mass per unit length of 1720 g mol-1 nm-1. Very similar values for these parameters, which refer to the secondary structure of the RNA molecule, were also derived from the X-ray scattering curves obtained in the presence of different amounts of Mg2+ (0.07 to 1 ions per nucleotide). On the contrary, the inner part of the X-ray scattering curves turned out to be highly dependent on the Mg2+ concen­tration: the cross-section radius of gyration and the mass per unit length, which were determined from the scattering curves at small angles as parameters related to the tertiary structure of the RNA, amounted to 3.11 nm and 4000 g mol-1 nm-1, respectively, in the absence of Mg2+ and in­creased significantly upon raising the concentration of Mg2+. The increase of these structural parameters was found to be accompanied by a decrease of the overall radius of gyration (as revealed indirectly by X-ray scattering and directly by light scattering measurements) and by an increase of the sedimentation coefficient. The results from the investigations of the RNA at low temperature clearly establish the existence of double-stranded structures down to very low Mg2+ concentrations as well as the occurrence of Mg2+ induced changes of the tertiary structure. In the absence of Mg2+ the RNA molecule appears to be a rodlike particle of about 300 nm length with a lateral diameter of about 9 to 11 nm. Upon addition of increasing amounts of Mg2+ this extended structure tightens, eventually forming a coil-like particle. Previous X-ray scattering experiments with MS2 RNA at ionic strength 0.1 (Zipper, Folkhard and Clauwaert, FEBS Lett. 56, 283-287 (1975)) have already established the axial dimensions of such coil-like particles to be about 63, 31 and 14 nm. The results from supplementary X-ray scattering experiments performed at higher temperatures in the absence or presence of Mg2+ clearly reflect the loss of secondary structure due to melting of the RNA. This loss is, however, less pronounced at higher concentrations of Mg2+ .

EFFECT OF MASTICATION ON THE STRUCTURE OF MICROGEL PRESENT IN NATURAL RUBBER

2017 ◽  
Vol 90 (3) ◽  
pp. 445-454 ◽  
Author(s):  
Sébastien Rolere ◽  
Florian Deme ◽  
Jérôme Sainte-Beuve ◽  
Frédéric Bonfils
Keyword(s):  
Light Scattering ◽  
Natural Rubber ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Radius Of Gyration ◽  
Random Coils ◽  
Exclusion Chromatography ◽  
Aggregate Fractions

ABSTRACT Mechanical or thermal mastication experiments were performed on three commercial natural rubber (NR) samples of TSR10 grade made from latex of three different clones (GT1, PB235, and RRIM600). The mesostructure (different gel or aggregate fractions, structure of random coils of cis-1,4-polyisoprene) of all the NR samples was fully characterized by size exclusion chromatography coupled with multiangle light scattering (SEC-MALS), using pretreated SEC columns. This method was used to quantify and investigate the structure of the little-studied smaller microaggregates, constituting the microgel fraction smaller than 1 μm (Microgel<1μ) of NR. The three unmasticated NR samples showed no difference in terms of microaggregate structure. Conversely, microaggregates appeared denser after mastication. This phenomenon was found to depend on the mastication conditions, as mechanically masticated NR samples had smaller (lower radius of gyration) and more compact microaggregates than thermally masticated samples. Macrogel also behaved differently depending on the mastication conditions. Mechanical mastication conditions allowed a higher degradation of the macrogel compared with thermal mastication conditions.

scholarly journals Conformational Ensembles by NMR and MD Simulations in Model Heptapeptides with Select Tri-Peptide Motifs

2021 ◽  
Vol 22 (3) ◽  
pp. 1364
Author(s):  
V. V. Krishnan ◽  
Timothy Bentley ◽  
Alina Xiong ◽  
Kalyani Maitra
Keyword(s):  
Principal Component ◽  
Md Simulations ◽  
Conformational Space ◽  
Random Coil ◽  
Radius Of Gyration ◽  
Small Peptides ◽  
Conformational Ensembles ◽  
Solution State ◽  
Peptide Motifs ◽  
Explicit Solvent

Both nuclear magnetic resonance (NMR) and molecular dynamics (MD) simulations are routinely used in understanding the conformational space sampled by peptides in the solution state. To investigate the role of single-residue change in the ensemble of conformations sampled by a set of heptapeptides, AEVXEVG with X = L, F, A, or G, comprehensive NMR, and MD simulations were performed. The rationale for selecting the particular model peptides is based on the high variability in the occurrence of tri-peptide E*L between the transmembrane β-barrel (TMB) than in globular proteins. The ensemble of conformations sampled by E*L was compared between the three sets of ensembles derived from NMR spectroscopy, MD simulations with explicit solvent, and the random coil conformations. In addition to the estimation of global determinants such as the radius of gyration of a large sample of structures, the ensembles were analyzed using principal component analysis (PCA). In general, the results suggest that the -EVL- peptide indeed adopts a conformational preference that is distinctly different not only from a random distribution but also from other peptides studied here. The relatively straightforward approach presented herein could help understand the conformational preferences of small peptides in the solution state.

scholarly journals Inspection of Transparent Objects with Varying Light Scattering Using a Frangi Filter

2021 ◽  
Vol 7 (2) ◽  
pp. 27
Author(s):  
Dieter P. Gruber ◽  
Matthias Haselmann
Keyword(s):  
Light Scattering ◽  
Machine Vision ◽  
Nearest Neighbor ◽  
Production Testing ◽  
Transparent Objects ◽  
Frangi Filter ◽  
Anomaly Score ◽  
Difference Images ◽  
New Machine

This paper proposes a new machine vision method to test the quality of a semi-transparent automotive illuminant component. Difference images of Frangi filtered surface images are used to enhance defect-like image structures. In order to distinguish allowed structures from defective structures, morphological features are extracted and used for a nearest-neighbor-based anomaly score. In this way, it could be demonstrated that a segmentation of occurring defects is possible on transparent illuminant parts. The method turned out to be fast and accurate and is therefore also suited for in-production testing.

scholarly journals Comprehensive characterization and quantification of adeno associated vectors by size exclusion chromatography and multi angle light scattering

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nicole L. McIntosh ◽  
Geoffrey Y. Berguig ◽  
Omair A. Karim ◽  
Christa L. Cortesio ◽  
Rolando De Angelis ◽  
...  
Keyword(s):  
Light Scattering ◽  
Size Exclusion Chromatography ◽  
Molar Mass ◽  
Size Exclusion ◽  
Minimal Sample ◽  
Accuracy And Precision ◽  
Vector Genome ◽  
Exclusion Chromatography ◽  
Novel Applications

AbstractAdeno associated virus (AAV) capsids are a leading modality for in vivo gene delivery. Complete and precise characterization of capsid particles, including capsid and vector genome concentration, is necessary to safely and efficaciously dose patients. Size exclusion chromatography (SEC) coupled to multiangle light scattering (MALS) offers a straightforward approach to comprehensively characterize AAV capsids. The current study demonstrates that this method provides detailed AAV characterization information, including but not limited to aggregation profile, size-distribution, capsid content, capsid molar mass, encapsidated DNA molar mass, and total capsid and vector genome titer. Currently, multiple techniques are required to generate this information, with varying accuracy and precision. In the current study, a new series of equations for SEC-MALS are used in tandem with intrinsic properties of the capsids and encapsidated DNA to quantify multiple physical AAV attributes in one 20-min run with minimal sample manipulation, high accuracy, and high precision. These novel applications designate this well-established method as a powerful tool for product development and process analytics in future gene therapy programs.

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