A Wound Contraction Experimental Model for Studying Keloids and Wound-healing Modulators

2003 ◽  
Vol 27 (8) ◽  
pp. 701-705 ◽  
Author(s):  
Fabio Kamamoto ◽  
Andre Oliveira Paggiaro ◽  
Andrea Rodas ◽  
Marisa Roma Herson ◽  
Monica Beatriz Mathor ◽  
...  
Keyword(s):  
Wound Healing ◽  
Experimental Model ◽  
Wound Contraction

scholarly journals Potential Wound Healing Properties of a Polyherbal Formulation (Amrit oil) in an Experimental Model on Wistar Rats

Biomedicine ◽  
2020 ◽  
Vol 39 (1) ◽  
pp. 40-51
Author(s):  
R Malarvizhi ◽  
Poddar Abhishek ◽  
R Barathidasan ◽  
Kanuga Kishore K. ◽  
HR Vasanthi
Keyword(s):  
Wound Healing ◽  
Experimental Model ◽  
Wound Repair ◽  
Wistar Rats ◽  
Wound Contraction ◽  
Control Group ◽  
Histopathological Analysis ◽  
Standard Drug ◽  
Polyherbal Formulation ◽  
Group I

Introduction and Aim: Wound healing is an integrated process of cellular and biochemical events in restoring the structural functionality of the damaged tissue caused due to wounds. In the present study, the wound healing potential of an ayurvedic polyherbal formulation, Amrit oil is tested on an experimental model of the excisional wound in Wistar rats and evaluated for its potent activity in wound repair. Materials and Methods: Wistar rats were randomly arranged into 4 groups with 6 in each. Excision wound was created on the dorsal side of the rats. Group-I was kept as a negative control, Group-II as reference control, treated with Betadine, Group-III received a tropical application of Amrit oil once in a day and Group- IV were subjected to tropical application of amrit oil twice or Bis in a day. The wound healing was assessed by percentage wound contraction, a period of epithelisation, histopathological analysis and expression of MMP- 2, MMP-8, KGF and HSP-90. Results: The effect produced by the ayurvedic polyherbal formulation (Amrit oil), in terms of percentage wound contraction, a period of epithelisation, biochemical gene expression (MMP2, MMP8, HSP90 and KGF) and histopathological analysis were comparable to that of standard drug betadine. This study reveals the healing potential of Amrit oil as compared to the positive control, betadine possibly due to the presence of the phytochemicals in a synergistic manner. Conclusion: This study revealed a tremendous healing potential of Amrit oil as compared to the positive control, betadine in a holistic manner, confirming the rationality of the traditional knowledge.      

scholarly journals Evaluation of wound healing and antimicrobial properties of aqueous extract from Bowdichia virgilioides stem barks in mice

2013 ◽  
Vol 85 (3) ◽  
pp. 945-954 ◽  
Author(s):  
ISABELA K.R. AGRA ◽  
LUANA L.S. PIRES ◽  
PAULO S.M. CARVALHO ◽  
EURIPEDES A. SILVA-FILHO ◽  
SALETE SMANIOTTO ◽  
...  
Keyword(s):  
Wound Healing ◽  
Latin American ◽  
Aqueous Extract ◽  
Antimicrobial Properties ◽  
Inflammatory Cells ◽  
Stem Bark ◽  
Wound Contraction ◽  
Type I ◽  
Folk Remedy

The decoction of the stem barks from Bowdichia virgilioides KUNTH is a folk remedy used to treat inflammatory disorders in Latin American and Brazil. In the present study, the wound healing activity of aqueous extract of the stem bark from B. virgilioides, called AEBv, was evaluated by the rate of healing by wound contraction and period of epithelization at different days post-wound using the wound excisional model. On day 9, the AEBv-treated animals exhibited significative reduction in the wound area when compared with controls. In wound infected with S. aureus, the AEBv significantly improved the wound contraction when compared to the saline-treated mice. The histological analysis showed that AEBv induced a collagen deposition, increase in the fibroblast count and few inflammatory cells than compared to saline-treated group. The expression of collagen type I was increased in the group treated with AEBv as indicated by immunohistochemical staining. In vitro, the AEBv was effective only against S. aureus but not against P. aeruginosa. Together, the results of this study demonstrate, for the first time, the healing and antimicrobiological effects of aqueous extract of the stem bark from B. virgilioides in the therapy of skin wounds.

scholarly journals Silk derived formulations for accelerated wound healing in diabetic mice

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10232
Author(s):  
Muniba Tariq ◽  
Hafiz Muhammad Tahir ◽  
Samima Asad Butt ◽  
Shaukat Ali ◽  
Asma Bashir Ahmad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Chronic Wounds ◽  
Histological Analysis ◽  
Healing Process ◽  
Aloe Vera ◽  
Wound Contraction ◽  
Wound Dressings ◽  
Control Group ◽  
Diabetic Mice

Background The present study aimed to prepare effective silk derived formulations in combination with plant extract (Aloe vera gel) to speed up the wound healing process in diabetic mice. Methods Diabetes was induced in albino mice by using alloxan monohydrate. After successful induction of diabetes in mice, excision wounds were created via biopsy puncture (6 mm). Wound healing effect of silk sericin (5%) and silk fibroin (5%) individually and in combination with 5% Aloe vera gel was evaluated by determining the percent wound contraction, healing time and histological analysis. Results The results indicated that the best biocompatible silk combination was of 5% silk fibroin and 5% Aloe vera gel in which wounds were healed in 13 days with wound contraction: 98.33 ± 0.80%. In contrast, the wound of the control group (polyfax) healed in 19 day shaving 98.5 ± 0.67% contraction. Histological analysis revealed that the wounds which were treated with silk formulations exhibited an increased growth of blood vessels, collagen fibers, and much reduced inflammation. Conclusion It can be concluded that a combination of Bombyx mori silk and Aloe vera gel is a natural biomaterial that can be utilized in wound dressings and to prepare more innovative silk based formulations for speedy recovery of chronic wounds.

scholarly journals EVALUATION OF WOUND HEALING ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII (A. DC.) IN WISTAR ALBINO RATS

2014 ◽  
Vol 1 (1) ◽  
pp. 73-77
Author(s):  
Uma Maheswari P ◽  
Shalimol A ◽  
Arumugasamy A ◽  
Udhaya Sankar M.R
Keyword(s):  
Wound Healing ◽  
Dry Weight ◽  
Wound Contraction ◽  
Albino Rats ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Model ◽  
Wistar Albino Rats ◽  
Incision Wound ◽  
Wound Healing Activity

The present experimental study was designed to evaluate the wound healing activity of methanolic extract of Smilax wightii A. DC. on incision and excision wound models in Wistar albino rats. The parameters studied were wound breaking strength, wound contraction area, epithelialization period, granulation tissuewet, dry weight and hydroxyproline content in incision wound model, percentage of wound contraction and period of epithelialization in excision wound model. The rats were administered topically with 100mg/kg b.wt. (low dosage), 200mg/kg b.wt. (moderate dosage) and 500mg/kg b.wt. (high dosage) of methanolicextract of Smilax wightii (MESW). The activity of the extract treated groups were compared with that of the control 1% Spirit. Framycetin sulphate 0.2% w/w was used as the standard drug. In incision wound model, there was a significant increase in the wound breaking strength in all the experimental groups treated withMESW than that of the control. Similarly, significant (P<0.001) decrease in wound contraction area and period of epithelialization were also observed in the test group animals treated with MESW and the standard drug treated groups when compared to that of the control. , a significant increase was observed in granulation tissue wet and dry weight and hydroxyproline content in the test groups treated with MESW compared to the control. In exicision wound model, there was a significant increase (P<0.01) in the percentage of wound contraction and decrease in period of epithelialization in the experimental groups treated with 200mg/kgb.wt. (moderate dosage) and 500mg/kgb.wt (high dosage) of MESW. The extract treated groups showed significant improvement in all the wound healing parameters of incision, and excision wound models.

Porcine Eyes as an Experimental Model for Wound Healing

2000 ◽  
Vol 16 (3) ◽  
pp. 381-381
Author(s):  
Ashley Behrens ◽  
Roy S Chuck ◽  
Michael R Bryant
Keyword(s):  
Wound Healing ◽  
Experimental Model ◽  
Porcine Eyes

EVALUATION OF EXCISIONAL AND INCISIONAL WOUND HEALING ACTIVITY OF ELECTROHOMEOPATHIC DRUG (SPAGYRIC ESSENCE) GREEN ELECTRICITY IN RATS

2020 ◽  
pp. 72-75
Author(s):  
PRASANT KUMAR SABAT ◽  
SWETA PRIYADARSINI PRADHAN ◽  
RANDEEP PATRO
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wound Contraction ◽  
Control Group ◽  
Albino Rats ◽  
Green Electricity ◽  
Wound Model ◽  
Wound Protection ◽  
Incision Wound ◽  
Wound Healing Activity

Objective: The current research is an attempt to evaluate the wound healing activity of Electrohomeopathic drug Green electricity (GE) in Wistar rats. No data have been reported till date on the wound healing effect of GE. Hence the present research focuses on the scientific investigation of the wound healing activity of GE in rats. Methods: Excision and incision wounds were inflicted upon albino rats. In the excision wound model, the wound contraction, Scar residue, area and time of complete epithelization were measured subsequently on day 0, 3,6,9,12,15, 18 and 21 after inflicting the wound. Wound contraction was calculated as a percentage change in the initial wound size. In the incision wound model, the tensile strength was measured on the 10th post wounding day. Results: It was noted that the effect produced by the GE Showed significant wound healing (p<0.01) in all wound models when compared to the control group. In the case of the excision model, GE statistically increases the wound contraction rate and in the case of the incision model GE showed high tensile strength as compared to the control group. In both cases the epithelization period was significantly decreased as compared to control. Almost 100% wound protection was achieved by GE in 21 d as compared to control. Conclusion: GE (ointment) showed significant wound healing activity (p<0.01) in both the excised wound model and incision wound model in rats.

scholarly journals Solanum incanum extract enhances wound healing and tissue regeneration in burn mice model

2019 ◽  
Vol 14 (2) ◽  
pp. 101-106
Author(s):  
Zainab Qureshi ◽  
Taous Khan ◽  
Abdul Jabbar Shah ◽  
Fazli Wahid
Keyword(s):  
Wound Healing ◽  
Tissue Regeneration ◽  
Histological Analysis ◽  
Negative Control ◽  
Wound Contraction ◽  
Mice Model ◽  
Wound Area ◽  
Solanum Incanum ◽  
Partial Thickness ◽  
Partial Thickness Burn

This study was conducted to evaluate the topical efficacy of Solanum incanum for the treatment of partial-thickness burn in mice model. Mice were treated with topical ointment of S. incanum three times daily for 14 days. The wound healing was observed through wound contraction and histological parameters. The group treated with S. incanum ointment showed 81% reduction in wound area as compared to negative control where wound area reduced to 22%. The histological analysis further confirmed that ointment favors the tissue regeneration and reepithelization thus heal wound rapidly as com-pared to other groups. In conclusion, S. incanum extract enhances wound healing and tissue regeneration.

Bromelain Encapsulated in Self Assembly Nanoemulsion Exhibits Better Debridement Effect in Animal Model of Burned Skin

2016 ◽  
Vol 40 ◽  
pp. 158-166 ◽  
Author(s):  
Heni Rachmawati ◽  
Evi Sulastri ◽  
Maria Immaculata Iwo ◽  
Dewi Safitri ◽  
Annisa Rahma
Keyword(s):  
Wound Healing ◽  
Animal Model ◽  
Vitamin E ◽  
Enzymatic Activity ◽  
Wound Contraction ◽  
Burn Treatment ◽  
Vitamin E Acetate ◽  
Burned Skin ◽  
Gel Preparation ◽  
The Stability

Bromelain is a mixture of proteolytic enzymes presence in all tissues of pineapple (Ananas comosus). It is known for clinical use as debridement for burn treatment. However, it is easily degraded by light, high temperature and pH. Nanoemulsion of bromelain is promising to increase its stability. In this study, we investigated the nanoemulsion of bromelain and its formulation into gel preparation in order to increase its efficacy for the burn treatment. Spontaneous or self-nanoemulsifying was applied to form nanoemulsion of bromelain (NEB). Bromelain was incorporated in various types of oil phase i.e virgin coconut oil (VCO), olive oil, vitamin E acetate and combination of both vitamin E acetate and VCO. Cremophor RH 40 was used together with polyethyleneglycol 400 to reduce oil-water interface tension. The stability of NEB in different oil phases was evaluated including particle size, polydispersity index, zeta potential, enzymatic activity and nanoemulsion morphology. Further, the most stable NEB was incorporated into hydrophilic gel matrix. An in vivo evaluation was carried out in hot plate-induced burn skin of New Zealand rabbit. Treatment of wounds was given by applying the preparations: NEB and the nanoemulsion bromelain-CMC gel (GKNB), using a standard protocol. As a control, untreated rabbit burned skin was provided. The efficacy of NEB was evaluated by observing wound contraction, eschar score, erythemic score, pus score and edema. After 14 days of storage, nanoemulsion using vitamin E acetate was found to be the most appropriate formula to encapsulate bromelain with good physical and chemical stabilities. This formula shows clear visual appearance with globule diameter of 74.37 nm, narrow size distribution, high loading efficiency of 97.96 %, and ability to maintain the enzymatic activity of bromelain compared to gel preparation using corresponding bromelain nanoemulsion. The vitamin E acetate nanoemulsion system shows better reduction in wound contraction until the 14th day of observation as well as other relevant parameters for wound healing effects. Taken together, bromelain formulated with the vitamin E acetate nanoemulsion improved the stability of bromelain and showed better activity to heal burnt skin on the animal model tested. The gel matrix retained the release of bromelain resulting in lower wound healing effect but it may have prolonged activity.

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