scholarly journals Potential Wound Healing Properties of a Polyherbal Formulation (Amrit oil) in an Experimental Model on Wistar Rats

Biomedicine ◽  
2020 ◽  
Vol 39 (1) ◽  
pp. 40-51
Author(s):  
R Malarvizhi ◽  
Poddar Abhishek ◽  
R Barathidasan ◽  
Kanuga Kishore K. ◽  
HR Vasanthi
Keyword(s):  
Wound Healing ◽  
Experimental Model ◽  
Wound Repair ◽  
Wistar Rats ◽  
Wound Contraction ◽  
Control Group ◽  
Histopathological Analysis ◽  
Standard Drug ◽  
Polyherbal Formulation ◽  
Group I

Introduction and Aim: Wound healing is an integrated process of cellular and biochemical events in restoring the structural functionality of the damaged tissue caused due to wounds. In the present study, the wound healing potential of an ayurvedic polyherbal formulation, Amrit oil is tested on an experimental model of the excisional wound in Wistar rats and evaluated for its potent activity in wound repair. Materials and Methods: Wistar rats were randomly arranged into 4 groups with 6 in each. Excision wound was created on the dorsal side of the rats. Group-I was kept as a negative control, Group-II as reference control, treated with Betadine, Group-III received a tropical application of Amrit oil once in a day and Group- IV were subjected to tropical application of amrit oil twice or Bis in a day. The wound healing was assessed by percentage wound contraction, a period of epithelisation, histopathological analysis and expression of MMP- 2, MMP-8, KGF and HSP-90. Results: The effect produced by the ayurvedic polyherbal formulation (Amrit oil), in terms of percentage wound contraction, a period of epithelisation, biochemical gene expression (MMP2, MMP8, HSP90 and KGF) and histopathological analysis were comparable to that of standard drug betadine. This study reveals the healing potential of Amrit oil as compared to the positive control, betadine possibly due to the presence of the phytochemicals in a synergistic manner. Conclusion: This study revealed a tremendous healing potential of Amrit oil as compared to the positive control, betadine in a holistic manner, confirming the rationality of the traditional knowledge.      

scholarly journals Pharmacological Evaluation of Chrozophora tinctoria as Wound Healing Potential in Diabetic Rat’s Model

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Harikesh Maurya ◽  
Monika Semwal ◽  
Susheel Kumar Dubey
Keyword(s):  
Wound Healing ◽  
Disease Control ◽  
Povidone Iodine ◽  
Diabetic Rats ◽  
Control Group ◽  
Standard Drug ◽  
Group Iii ◽  
Group I ◽  
Elevated Blood Glucose Level ◽  
Pharmacological Potential

Objective. The study was designed to evaluate pharmacological potential of hydroalcoholic leaves extract of Chrozophora tinctoria intended for wound healing in diabetic rats’ model. Methods. The method used to evaluate the pharmacological potential of hydroalcoholic leave extract was physical incision rat model. In this model, cutting of the skin and/or other tissues with a sharp blade has been made and the rapid disruption of tissue integrity with minimal collateral damage was observed shortly. Animals used in the study were divided into four groups that consist of six animals in each group. Group I serves as normal control, Group II serves as disease control, Group III was used as standard treatment (Povidone iodine 50 mg/kg b.w.), and Group IV was used for test drug (C. tinctoria 50 mg/kg b.w.). Result. The hydroalcoholic leave extract of Chrozophora tinctoria has been significantly observed to heal the wound (98%) in diabetic rats within 21 days, while standard drug (Povidone iodine) healed the wound about 95% in the same condition. The oral dose (50 mg/kg b.w.) of Chrozophora tinctoria was also found to improve the elevated blood glucose level in comparison to disease control group, which increased after the oral administration of Streptozotocin. Conclusion. The Chrozophora tinctoria has significant wound healing potential in the animal having physically damaged tissue in diabetic condition.

scholarly journals Determination of wound healing activity of Shark liver oil using the excision wound model in Wistar rats

2020 ◽  
Vol 11 (4) ◽  
pp. 7301-7308
Author(s):  
Auti S A ◽  
Jat R K ◽  
Das S K ◽  
Shukla S T
Keyword(s):  
Wound Healing ◽  
Disease Control ◽  
Wistar Rats ◽  
Chronic Wounds ◽  
Wound Contraction ◽  
Standard Drug ◽  
Wound Area ◽  
Excision Wound ◽  
Shark Liver Oil ◽  
Wound Model

There are various promising preclinical models, such as in mice, wistar rats, rabbits and pigs, which can be utilized to initiate acute or chronic wounds. These can be persuaded by many distinctive techniques, with excision the most common. After determining a proper model for a study, investigators need to choose an appropriate and reproducible technique that will permit the monitoring of the wound improvement over time. In this study, the healing power of Shark Liver Oil Emulgel (SLO) in Wistar rats were analyzed by using the excision wound model. The shark liver oil was prepared as emulgel at a concentration of 5%,10% and 15%, respectively and both Standard drug(Povidone-Iodine Ointment USP 5% w/w-PI) and SLO is applied at a concentration of 1mg/mm2; Topically. The parameters integrated for the assessment of the effects of SLO were Relative body weight changes, wound area contraction in mm2, relative wound percentage and epithelialization time. Wound area contraction was measured on 1st, 3rd, 6th, 9th, 12th, 15th, 18th and 21st day. Wistar rats treated with SLO showed substantial variations in epithelialization period and improved wound contraction in the excision wound treatment as compared to disease control. The biochemical biomarkers like SOD(Superoxide dismutases), GSH(Reduced Glutathione)and LPO(Lipid peroxidase) in the treated group have shown a significant change in the improvement of wound healing. Histopathological studies and microscopic observations specify that the topical use of Shark liver oil Emulgel extensively improved wound contraction, collagenation and epithelialization with well-organized dermis devoid of inflammatory cells in contrast to disease control.

scholarly journals A Histopathological Study of Cisplatin-induced Acute Vascular Injuries in Vital Organs and Protective Effect of Achillea millefolium

2020 ◽  
pp. 56-69
Author(s):  
Zahra Eslamifar ◽  
Susan Sabbagh
Keyword(s):  
Single Dose ◽  
Protective Effect ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Achillea Millefolium ◽  
Control Group ◽  
Histopathological Study ◽  
Histopathological Analysis ◽  
Vascular Injuries ◽  
Group I

The aim was to study the protective effect of ethanolic extract of Achillea millefolium on acute vascular injuries induced by cisplatin in liver, heart and renal tissues 24 hour after administration and using histopathological surveys in wistar rats. 24 adult male wistar rats were randomly divided into four groups. Group I (control group) received physiological saline for 10 days. Animals of group II had single dose of injection of CP (cisplatin) (6 mg/kg, IP) on the ninth day. Group III received Achillea millefolium extract (250 mg/kg, gavage) for 10 consecutive days. Group IV had both Achillea millefolium extract (250 mg/kg, gavage) for 10 consecutive days and a single dose of injection of CP (6 mg/kg, IP) on the ninth day. Kidney, liver and heart organs were collected on 10th day from sacrificed rats and subjected to histopathological analysis. Then the possible histopathological vascular effects of cisplatin on liver, heart, kidney tissues and the protective effect of Achillea millefolium extract was analysed. Obtained data showed the vascular injuries in CP group as congestion of cardiac capillaries (p=0.00) and interstitial edema (p=0.03). In the kidney, shrinkage of glomeruli (p=0.04), widening of Bowman's space (p=0.04), dilatation of cortical capillaries (p=0.01) were significantly altered. The findings of liver organ were increased sinusoidal space (p=0.00) and infiltration of neutrophils in portal space (p=0.01). Pretreatment with ethanolic extract of Achillea millefolium could attenuate these vascular injuries. Briefly, 24 hour after single injection of cisplatin the inflammatory process was seen in vital organs and administration of Achillea millefolium could mitigate these side effects.

scholarly journals Evaluation of Wound Healing Activity of an Ethanolic Extract of Anacardiumoccidentale Leaves in Wistar Rats

2020 ◽  
Vol 13 (4) ◽  
pp. 2061-2068
Author(s):  
Meena Kumari K ◽  
Amberkar Mohanbabu Vittalrao ◽  
Praveen Kumar SE ◽  
Sushma Prabhath ◽  
Charitha Charitha
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Histopathological Examination ◽  
Ethanolic Extract ◽  
Wound Contraction ◽  
Saline Control ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Healing Activity

BACKGROUND -This study undertaken to screen a potential wound healing activity of an ethanolic extract of Anacardiumoccidentale(Ao)leaves in Wistar rats. MATERIALS AND METHODS-Excision wound of 500mm2created on the back of Wistar rats. A total of thirty adult Wistar rats were prescreened and divided into five groups of six rats each.Group I and II rats were treated by normal saline (control) and povidone-iodine (standard drug), respectively. Group III and IV with 0.5% and 2%of Anacardiumoccidentale ointment (test drugs), respectively, and Group V rats treated with anti-wound healer drug dexamethasone, i.m. + 2% Anacardiumoccidentale. All drugs were applied topically except Inj. dexamethasone. In the model of excision wound, the wound healing effect was determined by parameters such as the percentage of wound contraction, duration of epithelialization, and histopathological examination. RESULT-All drugs, povidone-iodine, 0.5%, and 2% of the Ao ointment treated group of rats showed a statistically significant increase (P<0.05) in the % of wound contraction on 5th and 6thdays Vs.Control rats. In contrast,povidone-iodine, 2% Ao ointment and (dexamethasone + 2% Ao)treated rats decreased the period of epithelialization which was statistically significant (P<0.05) compared to the control.However, the standard povidone-iodine treated rats showed a maximum increase in the percentage of wound contraction and minimum period of epithelialization, followed by a higher dose of 2% Ao extract. The plant extract 0.5% and 2% of Ao showeda significant increase inthe rate of wound contraction as compared to the standard drug povidone-iodine also. The combination of (2%Aoextract+dexamethasone) drug-treated rats decreased the rate of wound contraction on 5th, 9th days; whereas, povidone-iodine increased on all days, i.e., 5th, 9th, 13th and 17th days,which was statistically significant (p<0.05) Vs.Normal saline. The povidone and 2% of Ao extract hastened the rate of wound healing activity, which was evident by maximum re-epithelialization and presence of the collagen fibers. CONCLUSION-In our study, the topical application of Anacardiumoccidentale leaf extract has shown promising wound healing activity; however, it failed to reverse the anti-wound healing property of steroids.

scholarly journals Unripe Rind and Pulp of Ananas comosus Accelerate Wound Healing in Diabetic Wistar Rat

2019 ◽  
pp. 1-13
Author(s):  
Rotimi Sunday Ajani ◽  
Nurudeen Abiola Opadokun
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Foot Ulcer ◽  
Wistar Rat ◽  
Wound Contraction ◽  
Ananas Comosus ◽  
Control Group ◽  
Accelerate Wound Healing ◽  
The Mean ◽  
Diabetic Wounds

Objective: One of the sequelae of diabetes mellitus is chronic foot ulcer. This study investigated wound healing propensity of the pulp and rind of Ananas comosus (a widely cultivated fruit with many health benefits) in diabetic wistar rats. Methods: The excisional wounds of twenty four adult wistar rats with induced diabetes in four groups of six each were dressed with the pulp powder (PPD), pulp extract (PED), rind powder (RPD) and rind extract (RED) of A. comosus daily till healed. Also the mean wound contraction rates were calculated from the wound areas every three days. Granulation tissue was biopsied from an animal per group on day 3, 6 and 9 for histopathological evaluation. Each of these groups had a corresponding equal number control group with similar procedures performed. Results: The mean wound contraction rates of the rind pulp diabetic (RPD) and rind extract diabetic (RED) groups paralleled those of their respective control group (RPC, REC) as they were not significantly different. Also amongst the diabetic groups, the rind of A. comosus demonstrated greater wound healing capability over the respective pulp counterpart (PPD, PED) as evidenced by significantly higher mean wound contraction rates. Histopathologic features of the granulation tissues and scars of the diabetic groups were similar to those of the control groups. Conclusion: Although, both the rind and pulp of unripe A. comosus either in extract or powder formulation were shown in this study to possess excellent healing potentials in diabetic wounds, the rind appeared to be better off and may be a viable alternative to the wound dressing materials currently in use in the management of diabetic wounds / ulcers.

scholarly journals Evaluation of the Wound Healing Potential ofResina Draconis (Dracaena cochinchinensis)in Animal Models

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Huihui Liu ◽  
Shaohui Lin ◽  
Dan Xiao ◽  
Xiao Zheng ◽  
Yan Gu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Histopathological Examination ◽  
Wound Contraction ◽  
Control Group ◽  
Traditional Use ◽  
Dragon’S Blood ◽  
Group I ◽  
Resina Draconis

Resina Draconis(RD) is a type of dragon's blood resin obtained fromDracaena cochinchinensis(Lour.) S.C. Chen (Yunnan, China). It has been used as a medicine since ancient times by many cultures. The ethanolic extract ofResina Draconis(RDEE) was evaluated for its wound-healing activity using excision and incision wound models in rats. Group I, the control group, was treated with ointment base. Group II, which served as a reference standard, was treated with moist exposed burn ointment (MEBO). Group III was treated with RDEE. The parameters observed were percentage of wound contraction, epithelialization period, tensile strength, histopathological studies, microvessel density (MVD), and the expression of vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1). The group treated with RDEE showed significantly better wound contraction and better skin-breaking strength as compared with the control group. The results of histopathological examination, MVD, and the expression levels of growth factors supported the outcome of the wound models as well. The present study provided a scientific rationale for the traditional use of RD in the management of wounds.

scholarly journals Evaluation of polyherbal ointment for wound healing activity in Wistar rats

2018 ◽  
Vol 8 (6-s) ◽  
pp. 26-31
Author(s):  
Shilpa S. Kolhe
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Breaking Strength ◽  
Skin Irritation ◽  
Wound Contraction ◽  
Alcoholic Extract ◽  
Polyherbal Formulation ◽  
Wound Healing Activity

The aim of present study to formulate, evaluate and to check the wound healing activity of newly prepared polyherbal formulation (ointment) .Herbal ointment containing hydrochloric extracts of plants Psorolea corylifalia, Achryanthes aspera ,was formulated as ointment and the hydro alcoholic extract was prepared by maceration method. These Formulations were evaluated for the following parameters: pH, Spread ability, grittiness, skin irritation study, stability. The wound healing activity is assessed by the rate of wound contraction, Period of epithelisation and skin breaking strength. Keywords: Herbal Ointment, Wound Healing Activity.

scholarly journals A topical ointment formulation containing leaves' powder of Lawsonia inermis accelerate excision wound healing in Wistar rats

2020 ◽  
Vol 13 (7) ◽  
pp. 1280-1287
Author(s):  
Kalbaza Ahmed Yassine ◽  
Hemida Houari ◽  
Benchohra Mokhtar ◽  
Amara Karim ◽  
Salem Hadjer ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Histological Study ◽  
Test Group ◽  
Wound Contraction ◽  
Control Group ◽  
Petroleum Jelly ◽  
Lawsonia Inermis ◽  
Wound Healing Activity ◽  
Daily Application

Aim: Lawsonia inermis (LI), a naturally grown or cultivated shrub in Northeast of Africa and India, has been traditionally used as a strong remedy for several injuries. However, few studies have reported its use as a cicatrizing agent. The aim of this study was to evaluate the effect of daily application of an ointment prepared with LI leaves' powder on wound healing in Wistar rats. Materials and Methods: Twenty female Wistar rats were used in this study. Excisional wound model was realized by removing skin from the dorsal part of the neck of each animal. Wounds have been then treated by a daily application of LI ointment prepared by mixing leaves' powder to petroleum jelly in test group and by simple application of petroleum jelly in control group. Evaluation of wound healing activity was then based on calculating the percentage of wound contraction, period of epithelialization, and wound index every 3 days for a period of 24 days, then, a histological study of the healed excised wound was performed. Results: Treatment with LI has shown excellent wound healing activity, since it has increased percent of wound contraction, and reduced period of epithelialization and wound index as compared to control (p<0.05). These results have been supported by the histological findings that revealed better epithelialization, dermal differentiation, collagen fiber orientation, and angiogenesis in LI treated rats compared to control (p<0.05). Conclusion: We can conclude that LI leaves' can be used as a potential wound healing agent.

scholarly journals Anticarcinogenic effect of probiotic fermented milk and chlorophyllin on aflatoxin-B1-induced liver carcinogenesis in rats

2011 ◽  
Vol 107 (7) ◽  
pp. 1006-1016 ◽  
Author(s):  
M. Kumar ◽  
V. Verma ◽  
R. Nagpal ◽  
A. Kumar ◽  
P. V. Behare ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Lactobacillus Rhamnosus ◽  
Thiobarbituric Acid ◽  
Fermented Milk ◽  
Hepatoprotective Effect ◽  
Control Group ◽  
Time Interval ◽  
Group I ◽  
Male Wistar Rats ◽  
Aflatoxin B

The present investigation was carried out to evaluate the hepatoprotective effect of probiotic fermented milk (FM) containing Lactobacillus rhamnosus GG and Lactobacillus casei strain Shirota, alone as well as in combination with chlorophyllin (CHL) as an antioxidant agent in male Wistar rats administered aflatoxin-B1 (AFB1). AFB1 was injected intraperitoneally at the rate of 450 μg/kg body weight per animal twice a week for 6 weeks, maintaining an equal time interval between the two consecutive AFB1 administrations. A total of 125 male Wistar rats were randomly allocated to five groups, each group having twenty-five animals. Group I was offered FM containing L. rhamnosus GG and L. casei strain Shirota. Group II was administered AFB1 and served as the control group; group III was administered FM-AFB1, in which besides administering AFB1, FM was also offered. Group IV was offered CHL and AFB1, and group V was offered both FM and CHL along with AFB1. The rats were euthanised at the 15th and 25th week of the experiment and examined for the biochemical and hepatopathological profile. A significant reduction in thiobarbituric acid-reactive substances (TBARS) was observed in the FM–CHL–AFB1 group compared with the AFB1 control group. FM alone or in combination with CHL was found to show a significant (P < 0·05) hepatoprotective effect by lowering the levels of TBARS and by enhancing the activities of antioxidant enzymes such as glutathione peroxidase, superoxide dismutase, catalase and glutathione-S-transferase, indicating that probiotic FM alone or in combination with CHL possesses a potent protective effect against AFB1-induced hepatic damage.

RNA Sequencing Reveals the Wound Repair Mechanism of Cuyuxunxi Prescription in Surgical Patients with Anal Fistulas

2021 ◽  
Vol 24 ◽  
Author(s):  
Yin Qu ◽  
Zhijun Zhang ◽  
Yafeng Lu ◽  
De Zheng ◽  
Wei Yang
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Rna Sequencing ◽  
Anal Fistula ◽  
Wound Repair ◽  
Inflammatory Responses ◽  
Control Group ◽  
Surgical Wound ◽  
Anal Fistulas ◽  
Skin Development

Background: Anal fistula is one of the most common colorectal and perirectal diseases in the world. Cuyuxunxi (CYXX) prescription is an efficient herbal fumigant used to promote the surgical wound healing of anal fistulas. Objective: This study aimed to explore the underlying molecular mechanism of CYXX prescription on surgical wound healing of anal fistulas. Methods: Ten patients with anal fistula were randomized into a control group or treatment group. The wound surface of patients in the control group was rinsed by normal saline, while that in the treatment group was rinsed by CYXX prescription. The wound tissues of patients with anal fistulas seven days after the surgery were collected for hematoxylin-eosin (HE) staining and RNA sequencing. The expressions of differentially expressed genes (DEGs) were validated by real-time quantitative PCR (RT-qPCR). Results: HE staining showed that CYXX treatment reduced the infiltration of inflammatory cells. A total of 472 DEGs, including 141 up-regulated genes and 331 down-regulated genes, were identified. These genes were significantly related to skin development, xenobiotic stimulus, and inflammation. In addition, the consistency rate of RT-qPCR and sequencing results was 83.33%, which showed a high relative reliability of the sequencing results. Conclusion: CYXX prescription could improve epidermis repair and reduce inflammatory responses.

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