scholarly journals Calpastatin (CAST) Gene Polymorphism in Tsigai and Merinoland Sheep Breeds Under Conditions of the Republic of Crimea

2021 ◽  
Vol 36 ◽  
pp. 06002
Author(s):  
Veronika Uppe ◽  
Tatiana Kuevda ◽  
Denis Zubochenko ◽  
Elena Usmanova ◽  
Pavel Ostapchuk
Keyword(s):  
Gene Polymorphism ◽  
Research Methods ◽  
Rflp Analysis ◽  
Primary Objective ◽  
Ongoing Study ◽  
Allelic Variants ◽  
Study Research ◽  
Pcr Rflp ◽  
The Republic ◽  
Cast Gene

The article presents the results of studying the Calpastatin (CAST) gene polymorphism in Tsigai (n = 23) and Merinoland sheep breed (n = 13). The purpose of the research. The possibility of using PCR-RFLP analysis to detect the Calpastatin (CAST) gene polymorphism in Tsigai and Merinoland sheep was the primary objective of this ongoing study. Research methods. Calpastatin (CAST) gene polymorphism was analyzed by PCR-RFLP analysis using the MspI endonuclease restriction. Results. We revealed the diversity of genotypes and allelic variants of the Calpastatin (CAST) gene in Tsigai and Merinoland Breeds. It was found that M allele of the Calpastatin locus is the most common. Frequencies of MM, MN and NN genotypes were found to be 74, 4 and 22 % in Tsigai breed. Among the representatives of Merinoland sheep, the frequencies of MM and MN genotypes were 92 % and 8%, respectively. No animals with NN genotypes were found.

scholarly journals CHARACTERISTIC OF MILK PRODUCTIVITY OF COWS-HEIFERS WITH DIFFERENT GENOTYPES OF SOMATOTROPIN (GH)

2019 ◽  
Vol 14 (3) ◽  
pp. 58-61
Author(s):  
Наталья Сафина ◽  
Natal'ya Safina ◽  
Ильназ Гилемханов ◽  
Il'naz Gilemhanov ◽  
Фарида Зиннатова ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Agricultural Production ◽  
Selection Criteria ◽  
Rflp Analysis ◽  
Analysis Method ◽  
Milk Productivity ◽  
Cattle Breeding ◽  
Pcr Rflp ◽  
Dairy Cattle Breeding ◽  
The Republic

. Polymorphisms of genes may be useful as genetic markers for additional selection criteria in dairy cattle breeding for economically advantageous traits. The presented study is dedicated to the influence of gene polymorphism, responsible for milk quality and milk productivity was carried out among Holstein cow-heifers in Integrated Agricultural Production Centre “Stud farm named after Lenin” of Atninsky district of the Republic of Tatarstan. DNA samples were isolated from blood samples of 130 cows for genotyping according to gene of growth hormone (GH) by PCR-RFLP analysis method. The genotypes distribution of the somatotropin gene were as follows: LL – 63.8% (83 animals), LV – 30.0% (39 animals), VV – 6.2% (8 animals), the distribution in frequency of occurrence of alleles L - 0.788 and V - 0.212. When considering the gene polymorphism association with the signs of milk production and the qualitative composition of milk showed prominent results cow-heifers with a LL genotype. The identified relationships indicate the possible genetic improvement of the investigated herds using marker-assisted selection.

scholarly journals Association of growth hormone (GH) gene polymorphism with growth and carcass in Sumba Ongole (SO) cattle

2017 ◽  
Vol 42 (3) ◽  
pp. 153 ◽  
Author(s):  
P. P. Agung ◽  
S. Anwar ◽  
W. P. B. Putra ◽  
M. S. A. Zein ◽  
A. S. Wulandari ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Gene Polymorphism ◽  
Fragment Length Polymorphism ◽  
Growth Parameters ◽  
Rflp Analysis ◽  
Cattle Breeding ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Polymerase Chain ◽  
Dressing Percentage

A study was conducted to identify the polymorphism in the intron 3 of the Growth Hormone (GH) gene and also to evaluate the association of the GH gene polymorphism with growth parameters and dressing percentage in the Sumba Ongole (SO) cattle. A total of 267 individual DNA samples were used in the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The SO cattle growth parameters data (n=44) including birth weight (BW), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365) and also dressing percentage (DP) (n=122) were investigated in this study. There were three genotypes (AA, AB, and BB) of the GH gene based on the PCR-RFLP analysis with allele frequency was 0.87 and 0.13 for A allele and B allele respectively. The highest genotype frequency in the SO cattle is AA (0.76) and the lowest is BB (0.02). The Heterozygosity Observed (Ho) value in the SO cattle population is 0.23 and Polymorphism Information Content (PIC) value is 0.20. Therefore, the genetic diversity in the SO cattle based on the GH gene polymorphism is quite low. There is no association (P>0.05) in BW, WW205, YW365, and DP with genotypes of the GH gene. As the result, the GH gene in this study cannot be used as a genetic marker in the SO cattle breeding program.

scholarly journals κ-casein polymorphism effect on technological properties of dried milk

2021 ◽  
Vol 9 (1) ◽  
pp. 95-105
Author(s):  
Ramil Vafin ◽  
Iskra Radaeva ◽  
Alexandr Kruchinin ◽  
Elena Illarionova ◽  
Alana Bigaeva ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Chemical Engineering ◽  
Raw Materials ◽  
Rflp Analysis ◽  
Technological Properties ◽  
Allelic Variants ◽  
Casein Gene ◽  
Milk Samples ◽  
Pcr Rflp ◽  
Reconstituted Milk

Introduction. Numerous molecular genetic studies have revealed a correlation between the polymorphism of milk protein genes and the technological properties of milk raw materials. DNA analysis, in particular, initiated research into the influence of allelic variants of κ-casein (CSN3) on thermal stability and cheese suitability of milk. This gives relevance to our study that compares the results of genotypic identification of lactating cows by the κ-casein gene, using raw and processed milk samples. Study objects and methods. Our study used raw and reconstituted milk samples from first-calf cows of the black motley breed with the AA and BB genotypes of the κ-casein gene. The samples were analyzed by standardized and generally accepted chemical engineering methods, as well as by capillary electrophoresis and PCR-RFLP analysis. Results and discussion. We compared the results of tests on thermal stability and cheese suitability of raw and reconstituted milk samples from cows with the AA and BB genotypes of the κ-casein gene. We tried out an integrated approach to monitoring milk raw materials based on the most relevant technological criteria and correlating the data with the associated CSN3 gene identification parameters. The PCR-RFLP analysis revealed reproducible results for both raw and dried milk samples in relation to the genotypical identification by the A- and B- allelic variants of the CSN3 gene. The tests showed higher thermal stability in the reconstituted milk from the BB genotype cow and better cheese suitability in the AA genotype sample. Conclusion. We developed a system for evaluating milk raw materials based on the most important technological parameters in combination with their genotypic characteristics. Our research procedure can unify the accumulation of experimental data and contribute to the formation of bioinformatics algorithms. This approach can be used in mathematical modeling of criteria to evaluate the compliance of the technological properties of milk with the recommended indicators.

scholarly journals Melanocortin 4 receptor (Mc4r) gene polymorphism and its association with body weights of some breeds of rabbit

2020 ◽  
Vol 47 (2) ◽  
pp. 1-7
Author(s):  
O. H. Osaiyuwu ◽  
U. F. O. Bolaji ◽  
O. A. Adeyinka ◽  
M. O. Akinyemi ◽  
A. E. Salako
Keyword(s):  
Body Weight ◽  
New Zealand ◽  
Gene Polymorphism ◽  
Rflp Analysis ◽  
The Body ◽  
Chi Square ◽  
Mc4r Gene ◽  
Fta Cards ◽  
Melanocortin 4 Receptor ◽  
Pcr Rflp

In livestock production, traditional methods of selection have always been the way to go. However, with the advent of genomics techniques, methods such as PCR-RFLP is been employed to identify single nucleotide polymorphism of likely candidate genes useful for livestock selection and improvement. Therefore, the aim of this study was to investigate the association of Melanocortin 4 Receptor (MC4R) gene polymorphism with the body weight ofrabbits. Seventy-four rabbits were used for this study consisting of six breeds; 26 Chinchilla, 3 Californian, 11 Dutch, 4 English Spot, 10 New Zealand White and 20 Fauve de Bourgogne (FDB) breeds. Blood samples were collected from the animals with needle and syringe and transferred unto FTA cards and stored away from light. Body weight measurements on the animals were recorded from 2 weeks to 20 weeks. PCR-RFLP analysis produced threegenotypes AA, AG, GG with genotype frequency of 0.14, 0.69 and 0.18 respectively. Allele frequency 0.48 and 0.52 for allele A and G respectively was obtained. Chi-square test showed that the population was not in Hardy-Weinberg equilibrium. Association analysis between the MC4R “c.101G>A” SNP and body weight of rabbit was tested using GLM procedure of SAS programme. Genotype GG had higher body weight (p<0.05) at 12, 16 and 20 weeks inthe Dutch rabbits while genotype AA recorded a higher (p<0.05) body weight value at 12 weeks in New Zealand White rabbits. More so, female Dutch rabbits had higher (p<0.05) mean weight than the males at 12, 16 and 20 weeks of age. Male New Zealand White breeds recorded higher (p<0.05) mean weight than the female animals. The result of the study showed that MC4R “c.101G>A” SNP was not associated with the body weight in the studied rabbit population, although, genotype AA had higher mean weight values at 12, 16 and 20 weeks of age than genotypes AG and GG. 

Kesalahan Pengucapan Diftong dan Vokal U pada Pidato Gubernur Jawa Tengah Ganjar Pranowo Dalam Rangka HUT Ke-74 Republik Indonesia

2020 ◽  
Author(s):  
Indana Lazulfa
Keyword(s):  
Data Analysis ◽  
Research Methods ◽  
Case Study Research ◽  
Speech Community ◽  
Study Research ◽  
Descriptive Research ◽  
Causes Of Errors ◽  
The Republic ◽  
Data Analysis Methods

ABSTRACTSpeech is one of the speaking skills that we often encounter in society. In a speech sometimes there is an error when talking. This study aims to describe the presence of errors in pronunciation of diphthongs and vowels u in Ganjar Pranowo's speech, also the causes of errors in pronunciation of diphthongs and vowels u. Mistakes of speech, of course, often occur among the speech community. Both intentional and unintentional mistakes. Data analysis methods used in this research are case study research and descriptive research methods. This method is very suitable and suitable for examining speech errors in the speech of Ganjar Pranowo in the 74th Anniversary of the Republic of Indonesia. The results of this study indicate that there were mistyping during the speech.Keywords:diphthongs, vocals, speech, language errors, speechesABSTRAK Pidato merupakan salah satu keterampilan berbicara yang sering kita jumpai dalam masyarakat. Dalam berpidato terkadang terdapat adanya kesalahan pada saat berbicara. Penelitian ini bertujuan untuk mendeskripsikan adanya kesalahan pengucapan huruf diftong dan vokal u pada pidato Ganjar Pranowo, juga penyebab terjadinya kesalahan pengucapan huruf diftong dan vokal u. Kesalahan berbicara tentu saja sering terjadi di antara masyarakat tutur. Baik kesalahan yang disengaja maupun tidak. Metode analisis data yang digunakan dalam penelitian ini adalah penelitian studi kasus dan metode penelitian deskriptif. Metode ini sangat sesuai dan cocok untuk mengkaji kesalahan berbicara pada pidato Ganjar Pranowo dalam Rangka HUT Ke-74 Republik Indonesia. Hasil penelitian ini menunjukkan adanya kesalahan pengucapan selama pidato tersebut. Kata kunci:diftong, vokal, berbicara, kesalahan berbahasa, pidato

Genetic polymorphisms of cytochrome P450 enzymes: CYP2C9, CYP2C19, CYP2D6, CYP3A4, and CYP3A5 in the Croatian population

2017 ◽  
Vol 32 (1) ◽  
Author(s):  
Lana Ganoci ◽  
Tamara Božina ◽  
Nikica Mirošević Skvrce ◽  
Mila Lovrić ◽  
Petar Mas ◽  
...  
Keyword(s):  
Rflp Analysis ◽  
The Other ◽  
Published Data ◽  
Cytochrome P450 Enzymes ◽  
Chain Reaction ◽  
Allelic Variants ◽  
Mediterranean Populations ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
European Populations

AbstractBackground:Data on the frequency of pharmacogenetic polymorphisms in the Croatian population are limited. We determined and analyzed frequencies for the most importantMethods:2637 subjects were included. Genotyping was performed by real-time polymerase chain reaction (PCR) using TaqMan® DME or TaqMan® SNP Genotyping Assays, and by PCR, and PCR-RFLP analysis.Results:ForConclusions:The frequency of the CYP allelic variants, genotypes, and predicted phenotypes in the Croatian population is in accordance with the other European populations, between the values of published data for Middle European and Mediterranean populations.

scholarly journals Analysis of calpastatin and сallipyge genes polymorphism in Prydniprovska meat sheep

2019 ◽  
Vol 6 (2) ◽  
pp. 58-65
Author(s):  
I. Pomitun ◽  
V. Rossokha ◽  
Ye. Boyko ◽  
O. Guzevatyi ◽  
M. Shpilka ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Muscle Hypertrophy ◽  
Restriction Endonucleases ◽  
Gene Fragment ◽  
Cross Breeding ◽  
Pcr Rflp ◽  
Meat Sheep ◽  
Cast Gene

Aim. To study calpastatin (CAST) and сallipyge (CLPG) genes polymorphism in Prydniprovska meat sheep. Methods. The studies were conducted using PCR-RFLP method. DNA was isolated from 47 animals. The amplicons were treated with restriction endonucleases MspI and FaqI for genes CAST and CLPG, respectively. Results. The study determined the polymorphism of CAST gene fragment. Two alleles – M (336, 286 b.p.) and N (622 b.p.) with the frequency of 0.83 and 0.17, respectively, were detected. The frequency of genotypes was as follows: ММ – 0.77, MN – 0.13 and NN – 0.10. There was a noted tendency towards the increase in live bodyweight of 4-month-old lambs, carriers of N allele (genotypes NN and MN), compared to the index for the lambs of the same age with genotype MM. Locus CLPG was monomorphic, only allele A was determined (278, 117 and 31 b.p.). Allele G with the mutation, manifested in muscle hypertrophy phenotype, was not detected, all the animals under investigation had genotype AA. Conclusions. CAST gene polymorphism was deter- mined in Prydniprovska meat sheep during our work. The tendency towards the increase in live bodyweight of 90-day-old lambs, carriers of allele N, was established which demonstrated promising perspectives of further studies on associations of this gene and meat qualities of Prydniprovska meat sheep. The obtained results on the monomorphic nature of locus CLPG and the absence of mutation, related to muscle hypertrophy phenotype, demonstrated that the mutation of this gene may be built into the genome of domestic breeds of sheep only via cross-breeding with foreign breeds, in which this trait is manifested.

scholarly journals THE IMAGE OF THE HUMAN PSYCHE IN RAUF PARFI POETRU

2021 ◽  
Vol 5 (3) ◽  
pp. 60-71
Author(s):  
Nigora Bozorova ◽  
Keyword(s):  
Research Methods ◽  
Spiritual Values ◽  
Study Research ◽  
Methodological Basis ◽  
The World ◽  
Human Psyche ◽  
The Republic

Introduction. The first President of The Republic of Uzbekistan theoretical views on Karimov's works on spiritual values, National ideas and ideology formed the methodological basis of this work. A number of literary scholars drew attention to the scientific-theoretical views of literature, literature of the period of independence, poetry of the period of independence on the creativity and life of Rauf Parfi, the peculiarities of Rauf Parfi's creativity, the world of images, genres and poetic forms, the proposals and comments expressed in them. Rauf Parfi's poetic collections, published in different years, served as an important source of internet materials for the study. Research methods.

scholarly journals Identification of KLF3 gene polymorphism in Indonesian Friesian Holstein cattle

2018 ◽  
Vol 43 (3) ◽  
pp. 195 ◽  
Author(s):  
P. P. Agung ◽  
F. Laoditta
Keyword(s):  
Gene Polymorphism ◽  
Rflp Analysis ◽  
Yield Traits ◽  
Biological Processes ◽  
Blood Samples ◽  
Central Java ◽  
Pcr Rflp ◽  
Initial Identification ◽  
Intron Region ◽  
Cattle Blood

The KLF3 gene was a potential genetic marker candidate due to its involvement in many biological processes such as cell proliferation, cell differentiation, and apoptosis. This study was conducted to identify the KLF3 gene polymorphism in the Indonesian FH cattle. A total of 302 individual cattle blood samples from West Java (n=138), Central Java (n=34), and East Java (n=130) provincies were used to obtainDNA samples. The DNA sequencing was performed using 9 samples (each location of sampling represented by 3 samples) for initial identification of the KLF3 gene polymorphismand followed by PCR-RFLP analysis using all DNA samples. There were two SNPs identified in the intron region of the KLF3 gene i.e. SNP g.59607486delC and SNP g.59607554A>G. The SNP g.59607554A>G could be detected using PCR-RFLP method with HpyCH4IV restriction enzyme and resulted three genotypes of the KLF3 gene (AA, AG, and GG) but its heterozygosity value was low in the Indonesian FH cattle. The AA genotype has the highest frequency (0.73), while the AGand GG genotypes frequency were 0.24 and 0.03, respectively. Information about the KLF3 gene polymorphism in this study can be considered for further study to analyse its association with milk and protein yield traits.

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