scholarly journals CHARACTERISTIC OF MILK PRODUCTIVITY OF COWS-HEIFERS WITH DIFFERENT GENOTYPES OF SOMATOTROPIN (GH)

2019 ◽  
Vol 14 (3) ◽  
pp. 58-61
Author(s):  
Наталья Сафина ◽  
Natal'ya Safina ◽  
Ильназ Гилемханов ◽  
Il'naz Gilemhanov ◽  
Фарида Зиннатова ◽  
...  

. Polymorphisms of genes may be useful as genetic markers for additional selection criteria in dairy cattle breeding for economically advantageous traits. The presented study is dedicated to the influence of gene polymorphism, responsible for milk quality and milk productivity was carried out among Holstein cow-heifers in Integrated Agricultural Production Centre “Stud farm named after Lenin” of Atninsky district of the Republic of Tatarstan. DNA samples were isolated from blood samples of 130 cows for genotyping according to gene of growth hormone (GH) by PCR-RFLP analysis method. The genotypes distribution of the somatotropin gene were as follows: LL – 63.8% (83 animals), LV – 30.0% (39 animals), VV – 6.2% (8 animals), the distribution in frequency of occurrence of alleles L - 0.788 and V - 0.212. When considering the gene polymorphism association with the signs of milk production and the qualitative composition of milk showed prominent results cow-heifers with a LL genotype. The identified relationships indicate the possible genetic improvement of the investigated herds using marker-assisted selection.

2017 ◽  
Vol 42 (3) ◽  
pp. 153 ◽  
Author(s):  
P. P. Agung ◽  
S. Anwar ◽  
W. P. B. Putra ◽  
M. S. A. Zein ◽  
A. S. Wulandari ◽  
...  

A study was conducted to identify the polymorphism in the intron 3 of the Growth Hormone (GH) gene and also to evaluate the association of the GH gene polymorphism with growth parameters and dressing percentage in the Sumba Ongole (SO) cattle. A total of 267 individual DNA samples were used in the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The SO cattle growth parameters data (n=44) including birth weight (BW), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365) and also dressing percentage (DP) (n=122) were investigated in this study. There were three genotypes (AA, AB, and BB) of the GH gene based on the PCR-RFLP analysis with allele frequency was 0.87 and 0.13 for A allele and B allele respectively. The highest genotype frequency in the SO cattle is AA (0.76) and the lowest is BB (0.02). The Heterozygosity Observed (Ho) value in the SO cattle population is 0.23 and Polymorphism Information Content (PIC) value is 0.20. Therefore, the genetic diversity in the SO cattle based on the GH gene polymorphism is quite low. There is no association (P>0.05) in BW, WW205, YW365, and DP with genotypes of the GH gene. As the result, the GH gene in this study cannot be used as a genetic marker in the SO cattle breeding program.


2020 ◽  
Vol 17 ◽  
pp. 00105
Author(s):  
Tahir M. Akhmetov ◽  
Natalia Yu. Safina ◽  
Shamil K. Shakirov

The study presents the results obtained in DNA testing of allelic polymorphism by PCR-RFLP based methods for the leptin and stearoyl-coenzyme A desaturase genes and the interaction of their complex genotypes on the milk productivity indicators of Holstein heifers of Tatarstan population. The study was carried out among 471 Holstein cow-heifers by Integrated Agricultural Production Centre “Stud farm named after Lenin” of Atninsky district of the Republic of Tatarstan in 2016–2018. All complex LEP/SCD1 genotypes were identified through the study. The maximum frequency was observed in the combination of heterozygous genotypes LEPTCSCD1TC 149 animals (31.5 %). Milk productivity test, based on the complex genotypes LEP and SCD1, showed that a significantly high level of productivity for 305 days of lactation was 7314.4 kg, and in heifers with a complex genotype LEPTTSCD1CC was minimal (6337.4 kg) in comparison with indicators of milk yield of heifers with other complex genotypes. According to the content of the mass fraction of fat, reliably the best indicators were established in a subpopulation of animals with a complex TT/TC genotype 3.87 %. The content of the mass fraction of protein was maximal for animals with a complex CC/CC genotype.


2014 ◽  
Vol 37 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Nikola Adamov ◽  
Dine Mitrov ◽  
Igor Esmerov ◽  
Peter Dovc

Abstract BLAD and CVM are Holstein-specific recessive mutations which have had significant economic impact on dairy cattle breeding worldwide. The aim of this study was to optimize a diagnostic test for detection of these two disorders and to obtain a preliminary picture about theire existence in Macedonian HF population. Blood samples were obtained from 84 HF cows from several different farms in Macedonia. Genomic DNA was extracted using DNeasy blood and tissue kit (Qiagen) which was also used for DNA extraction from deeply frozen sperm doses from 6 different HF bulls. Genotyping was performed by PCR-RFLP analysis with TaqI and HaeIII restriction endonucleases for BLAD and with PstI for CVM. The correctness of the genotyping was evaluated by direct sequencing of the amplified products. Among 90 HF cattle tested two cows were identified as carriers of the BLAD allele and one was carrier of the CVM allele. No bulls were found to be carriers of either of the two disorders. This study demonstrates that carriers of BLAD and CVM are present in Macedonia, although at low frequency. PCR followed by RFLP is efficient and inexpensive method for detection of the carriers and should be used as laboratory test in eradication programs. In order to establish a more accurate picture of the prevalence of these disorders, it is necessary to screen a larger number of animals. It is indispensable to test each bull before entering the A.I. program to prevent accidental transmission to a large number of offspring.


2021 ◽  
Vol 36 ◽  
pp. 06002
Author(s):  
Veronika Uppe ◽  
Tatiana Kuevda ◽  
Denis Zubochenko ◽  
Elena Usmanova ◽  
Pavel Ostapchuk

The article presents the results of studying the Calpastatin (CAST) gene polymorphism in Tsigai (n = 23) and Merinoland sheep breed (n = 13). The purpose of the research. The possibility of using PCR-RFLP analysis to detect the Calpastatin (CAST) gene polymorphism in Tsigai and Merinoland sheep was the primary objective of this ongoing study. Research methods. Calpastatin (CAST) gene polymorphism was analyzed by PCR-RFLP analysis using the MspI endonuclease restriction. Results. We revealed the diversity of genotypes and allelic variants of the Calpastatin (CAST) gene in Tsigai and Merinoland Breeds. It was found that M allele of the Calpastatin locus is the most common. Frequencies of MM, MN and NN genotypes were found to be 74, 4 and 22 % in Tsigai breed. Among the representatives of Merinoland sheep, the frequencies of MM and MN genotypes were 92 % and 8%, respectively. No animals with NN genotypes were found.


Author(s):  
K.ZH. ZHUMANOV ◽  
◽  
T.N. KARYMSAKOV ◽  
M.A. KINEEV ◽  
M.V. TAMAROVSKIY ◽  
...  

According to the current “Instruction” used in dairy cattle selection and breeding in the Republic of Kazakhstan, bulls-producers of dairy breeds are assessed according to the their offspring quality based on the principle of “peer daughter”. This means that the phenotypic indicators of the daughters of the tested bulls are compared with the corresponding indicators of their peers. In European countries with developed dairy cattle breeding, as well as in Canada, the USA, etc., to ensure a reliable forecast of the genetic value of individuals (primarily, bulls-producers), use is made of the best linear unbiased forecast method (BLUP method). This method implies that the breeding value of producers is determined by the deviation values of the development of traits of the examined animal from its average values in the population. Especially urgent area is the research aimed at improving breeding programs, including assessing the breeding value of bulls-producers of dairy breeds using BLUP methods based on the productive qualities of the mass of dairy cattle in the Republic of Kazakhstan. The research material included the data on the phenotypic indicators of the milk productivity of first-calf cows (the amount of milk yield, the content of fat and protein in milk, the yield of milk fat and protein) of the Holstein black-motley dairy cattle breed, obtained from the information and analytical database of the Republic of Kazakhstan for 2016–2017. It was found that when evaluating according to the official “Instruction”, 16 sires out of 256 bulls (6.2%) got the stud category in 2016, 14 sires (9.2%) out of 152 bulls in 2017, and – 30 sires of 249 bulls (12.0%) over the cumulative period. The results of the conducted research prove that the use of the classic “Instructions” in dairy cattle breeding has lower efficiency (by 42.8–90.0%) as compared with the assessment of the breeding value of bulls based on the BLUP method.The selection of sire bulls into breeding groups based on the “peer daughter” methodology is not reliable enough and rather ineffective. Comparing the results of assessing the breeding qualities of sire bulls, obtianed using two methods in all compared periods (2016, 2017, 2016–2017), the authors established a clear superiority of the BLUP method over the current Instruction used in the Republic of Kazakhstan.


In this article approaches to search for reserves of decrease in cost of agricultural production are considered. The methods of cost calculation of dairy cattle breeding products used at the studied enter-prise are analysed, short characteristic of the standard method offered by the Ministry of Agriculture is given, and calculations of alternative options are also carried out. Today creation of accounting of a production unit is very important so that not only weight units must be considered in it, but also the quali-tative structure of products must be reflected. Definition of qualitative characteristics and technological properties by production of milk which depend on use purposes can be an example. The raw materials consumption on a unit of production and its quality and also firmness of storage depends on technologi-cal properties of milk. At calculation of prime cost taking into account qualitative characteristics for cal-culation milk in terms of basic fat content undertakes. The method of calculation of prime cost consider-ing qualitative characteristics is the most expedient as prime cost of 1 c of milk unlike the operating tech-nique is lower. In the article analytical methods of reserves calculation for decrease in prime cost taking into account various factors are proved. The revealed reserves will allow an enterprise to expand its in-vestment opportunities in the future, they will give an additional incentive of modernization of the worn-out machinery and equipment in branches of agriculture.


Author(s):  
N. M. Kostomakhin ◽  
T. N. Pimkina ◽  
O. A. Voronkova ◽  
M. A. Gabedava ◽  
E. G. Cheremukha

Dairy cattle breeding have always occupied and will continue to occupy one of the leading places in the agro-industrial complex in our country. The most eff ective method of improving existing breeds according to opinion of a number of scientists is selection in line breeding. At the same time the evaluation of breeding sires by the quality of offspring is the cornerstone of breeding work. The purpose of the research was to analyze the influence of breeding sires, as well as their linear affiliation on the economically useful characteristics of first-calf heifers under specific conditions of maintenance and use. An analysis of the milk productivity and reproductive traits of first-calf heifers of different origin by sires and different line belonging has been presented in the article. The results of intra-linear selection and crosses of genealogical lines and their influence on the productive and reproductive traits of first-calf heifers have been evaluated. The research was carried out in LLC “Green Lines-Kaluga” of the Kaluga region. It has been established that for fixing in the genotype of the offspring of plentiful milk and fat milk in purebred breeding, it is necessary to use the following sires: Aragon 2102, Tabor 1292, Lira 2047, Phlox 1448, Chudny 1167, Yalik 397, Tabun 1277, as well as intra-linear selection in the line of Montwick Chieftain 9567929 and crosses of lines in combinations: Siling Traijun Rocket 252803 – Reflection Sovereign 198998 and Wes Ideal 933122 – Siling Traijun Rocket 252803. In order to reduce the age of the first insemination of maternal stock we recommend using a combination of lines: Wes Ideal 933122 – Montwick Chieftain 9567929, Wes Ideal 933122 – Reflection Sovereign 198998, Montwick Chieftain 9567929 – Wes Ideal 933122, Montwick Chieftain 9567929 – Reflection Sovereign 198998 and Siling Trijun Rocket 252803 – Wes Ideal 933122.


2020 ◽  
Vol 11 (2) ◽  
pp. 1511-1514
Author(s):  
Sergey Yu. Smolentsev ◽  
Galiya R Yusupova ◽  
Nikita V Nikolaev ◽  
Zaira F Aukhadieva ◽  
Renat A Volkov ◽  
...  

The effectiveness of dairy cattle breeding largely depends on the intensity of use of the breeding stock. At the same time, the factor of the duration of economic use of animals is gaining importance, which affects not only the production economy, but also the improvement of stocks and breeds. The lifelong productivity, the quantitative and qualitative growth of the stock, the amount of capital investment in its formation and the efficiency of operation depend on the term for the productive use of cows. The terms of breeding and production use of animals are direct linked with the biologically possible longevity of each animal, in turn, the duration of the productive period of cattle is in the range of 12-17 lactations. The influence of feed on production is associated primarily with the level of digestion and assimilation. The digestive capacity of the digestive tract of animals can be improved by selecting the optimal ratio in the diet of rough, juicy and concentrated feeds, i.e. optimization of animal feeding types. We conducted an analysis of milk productivity in lactating cows, when using AVMC feed additive in the diet. The experiment was conducted in the conditions of the dairy farm of Biryuli CJSC of the Vysokogorsky District of the Republic of Tatarstan on three groups of lactating cows. For the experiment, 30 cows were selected, 10 in each Holstein black-and-white breed according to the principle of analogues. Studies have shown that AVMC with rapeseed oil seeds and AVMC with fus in the diet of lactating cows provided higher animal productivity and positively affected the chemical composition of milk.


2020 ◽  
Vol 13 (10) ◽  
pp. 2046-2052
Author(s):  
I. Dolmatova ◽  
T. Sedykh ◽  
F. Valitov ◽  
R. Gizatullin ◽  
D. Khaziev ◽  
...  

Aim: This study aimed to determine the effect of thyroglobulin (TG5) gene polymorphism on milk and meat productivity in the various cattle breeds currently bred in the Republic of Bashkortostan. Materials and Methods: The test was performed on dairy cattle of Black-and-White, Bestuzhev, and Simmental breeds, and meat cattle of Hereford and limousine breeds. The purpose of the test was to search for associations between the polymorphic alleles of the thyroglobulin (TG5) gene and economically useful traits. Results: All studied breeds showed a frequency predominance of the TG5C allele (from 0.56 to 0.71). A clear trend of an effect of the genotypes of the TG5 gene on milk-productivity indicators was revealed; cows with the TG5TT genotype have the highest milk yield and fat content in milk. The milk of cows of Bestuzhev and Simmental breeds that possessed this genotype was also characterized by higher protein content. Conclusion: We identified an effect of the polymorphism of the TG5 gene in the Hereford and limousine breeds on fat metabolism intensity indicators, such as fat output and fat content, in the longissimus muscle and in the general sample of ground beef.


2019 ◽  
Vol 57 ◽  
pp. 60-67
Author(s):  
G. D. Іlyashenko

Dairy cattle breeding is one of the leading livestock sectors whose purpose is to ensure the production of high quality dairy raw materials in volumes meeting the scientifically grounded standards of state food security and expanding the export potential of the agrarian sector. The purpose of our research was to study the status and prospects of dairy cattle breeding in the Kirovograd region. Material and methods of research. The research was carried out on the basis of the electronic database of breeding goals (including 2312 cows), official statistics and estimates of cattle of various breeds that are involved in the production of dairy direction in the breeding farms of the Kirovograd region. The analysis used zootechnical and statistical methods. The calculations were carried out using the software package "STATISTICA-6,1" Results. According to the research (2001–2018), the cattle population in the Kirovograd oblast decreased by a factor of 2.6. Thus, as of January 1, 2018, its number constituted 96.3 thousand heads in all categories of farms, 53.1 thousand of them were cows, the number of which also decreased by 2.4 times. Specific gravity of cattle population of farms remains significant and makes up 72.7%, and 80.6% in the livestock of cows. The largest number of cows of agricultural enterprises declined significantly. Thus, as of January 1, 2018, their number was 10.3 thousand goals, which is 4.7 times less than in the same period of 2001. In the households there is also a tendency for a permanent reduction of cows, the difference is, respectively, 37.0 thousand heads or 46.4% The development of dairy cattle breeding is characterized by two opposite tendencies: reduction of livestock of cows and increase of their productivity. Thus, in agricultural enterprises in 2010, compared to 2000, hopes per cow increased by 2.5 times, in all categories of farms by 1.8 times. In 2017, the increase in dairy costs in agricultural enterprises compared to 2010 amounted to 60.0% in farms of all categories 12.2%. The researches found that in the agricultural enterprises of the oblast as of January 1, 2018 there are 26256 head of cattle, including 10281 cows. The breeding part of the existing cows is 3.013 heads, 29.3% of the total number located in 10 breeding farms. The analysis of the genealogy structure showed that the experimental population of cows belongs to 18 lines and related groups of which the most numerous Chifa 1427381 – 20.4%, Starbuck 352790 – 20.1%, Henev 1629391 – 18.6% , Eleusis 1491007 – 11.2%, Family 267150 – 10.8%. Among the indicated lines, the lowest level of milk productivity was first-born cows. The 1491007, the milk of milk for 305 days of lactation was 4466 ± 99.8 kg, and the milk fat was 168.2 ± 6.5 kg. Analogues l Starbak 352779 was characterized by the highest milk productivity, its level was 5696 + 98.8 kg and 224.9 ± 9.7 kg, respectively. The difference in stomach was 1230 ± 140.4 kg (td = 10.2) and at the release of milk fat 56.7 ± 5.28 kg (td = 10.2) at P < 0.001. However, the latter were markedly less service life (125 against 168 days) and logically higher reproduction rate compared to other lines. An analysis of the intensity of the formation of live weight of young animals has been established for its highest growth in heifers of controlled herds in the period from 9 to 12 months. Thus, in animals of Ukrainian red breed, the growth rate of live weight during this period was 75 kg, Ukrainian red-shrimp 61 kg, Ukrainian black-and-white 73 kg, Holstein 76 kg. Accordingly, during this period, the average daily gain of live weight of young breeds of these breeds was 833 g; 678 g; 811 g, 835 g. The sharp fluctuations of average daily increments during growing periods, first of all, testify to the unequal conditions of growing and feeding young animals than the breed differences. In general, as a result of the analysis of the formation of live weight of heifers, a significant level of phenotypic variability was observed, which confirms sufficient genetic resources and preconditions for selection on the given sign. Therefore, proceeding from the above, in the long run, selection work for breeding improvement of cattle breeding farms of the Kirovograd region should be conducted in accordance with certain promising directions according to groups of breeds. Conclusions. A very important factor in the growth of milk production in the Kirovograd region is high-yield pedigree cattle. The main task on the way of bringing breeding cases in dairy farming to the modern level is the introduction of computer breeding records in breeding farms. This will allow to estimate in the near future bulls-pedigrees, which were used in the past in these farms, to allocate among them complex improvers for precisely the specific conditions and maximally attract them for reproduction. On the other hand, a reliable clogging of poor quality in the genetic sense of sperm production, which leads to negative consequences, will be posed. When establishing the assessment of the breeding value of bulls and developing a single plan of breeding and breeding work in the region, one can proceed to the next step of improving breeding and breeding business, introducing a progressive method of raising the breeding value of animals of large-scale breeding. As a result, the profit from the breeding of highly productive animals, well adapted to specific conditions, will significantly exceed the costs. At the same time, the proposed measures will allow the breeding business to be brought up to the modern world level and stimulate highly productive cattle breeding.


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