Antioxidant and anticancer activities of methanolic, ethyl acetate and chloroform extracts of Arum Palaestinum

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
M Diab-Assaf ◽  
RI Taleb ◽  
W Shebaby ◽  
A Mansour ◽  
CJ Moussa ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Anticancer Activities

scholarly journals Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
K. Nagendra Prasad ◽  
Jing Hao ◽  
Chun Yi ◽  
Dandan Zhang ◽  
Shengxiang Qiu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Liver Carcinoma ◽  
Scavenging Activity ◽  
Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

scholarly journals Anti-Oxidant, Anti-Bacterial and Anti-Cancer Activity of Mentha Piperita Against Mcf-7 Cells

2021 ◽  
Vol 14 (3) ◽  
pp. 1685-1693
Author(s):  
Renuka Saravanan ◽  
Ravichandran Natesan ◽  
Sumathi C Samiappan ◽  
Sivakumar Ramalingam
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Bacillus Cereus ◽  
Cell Line ◽  
Bacterial Species ◽  
Mentha Piperita ◽  
Anticancer Activities ◽  
Leaf Extracts ◽  
Apoptotic Effect ◽  
Mcf 7

The study evaluates the antioxidant, antibacterial and anticancer activities of different extracts of Menthapiperitaleaves against the MCF-7 cell line. The leaves of Menthapiperitawere extracted with aqueous, ethyl acetate, and chloroform. These extracts were subjected to qualitative phytochemical screening, antibacterial activity, cytotoxic activity, and AO/ErBr assay for cells' apoptotic effect against the MCF-7 cell line. Qualitative analysis of the leaves' different extracts revealed glycosides, alkaloids, flavonoids, terpenoids, tannin, and saponin. The antibacterial activity of the leaf extracts was examined against four different bacterial species (Bacillus cereus, Pseudomonasfluorescens, Aeromonas hydrophila, and Klebsiella pneumoniae). The aqueous extract exhibited a high level of antibacterial activity (18.66mm ±1.1 in Bacillus cereus).Free‑radicalscavenging activity of chloroform extractof M. piperita leaves was found to be more than aqueous and ethyl acetate extracts. Further, aqueous, ethyl acetate,and chloroform extracts exerted a cytotoxic effect with the IC50value of 45±1.5μg/ml, 29±1.2μg/ml, and 24±1.0μg/ml, respectively.From this study, we have observed that chloroform extract showed a concentration-dependent apoptotic effect against MCF-7 cellsdetermined by AO/EtBr assay.The resultsfurther depicted that the selected traditionalMentha piperita could be used as a potential anticancer, antibacterial, and good antioxidant agent against the MCF-7 cell line.

scholarly journals FUNGICIDAL ACTIVITY OF GARLIC (Allium sativum) BULBS EXTRACTS AGAINST PLANTS PHATOGENIC FUNGI

2019 ◽  
Vol 19 (1) ◽  
pp. 23
Author(s):  
Khairan Khairan ◽  
Aulina Aulina ◽  
Muhammad Bahi ◽  
Cut Nova Eriana ◽  
Rina Sriwati
Keyword(s):  
Ethyl Acetate ◽  
Fusarium Oxysporum ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Anticancer Activities ◽  
Colletotrichum Capsici ◽  
Garlic Bulb ◽  
Paper Disc

Fungicidal activity of garlic (Allium sativum) bulbs extracts against plants phatogenic fungi. Garlic (Allium sativum Linn) has been known to containing organosulphur compounds. These compounds are convinced to possess antibacterial, antifungal, and anticancer activities. The aim of this study is to determine fungicidal activity of garlic bulb extracts against some plants phatogenic fungi. The paper disc agar difussion technique was used to determine fungicidal activity of garlic bulbs extracts. The results showed that in general garlic bulbs extracts provides fungicidal activities. Calculated EC50 values indicated that ethyl acetate garlic bulbs extract was most active against Colletotrichum capsici, Fusarium oxysporum f. sp.capsici, and Sclerotium rolfsi by EC50 values of 48,6; 50,3; and 51.3% respectively. Meanwhile methanolic garlic bulbs extract was the most active against Sclerotium rolfsi with EC50 values of 24,3%.

scholarly journals Antibacterial, antioxidant, cytotoxic effects and GC-MS analysis of mangrove-derived Streptomyces achromogenes tch4 extract

2021 ◽  
pp. 17-17
Author(s):  
Janpen Tangjitjaroenkun ◽  
Wanchai Pluempanupat ◽  
Rungnapa Tangchitcharoenkhul ◽  
Waraporn Yahayo ◽  
Roongtawan Supabphol
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Bioactive Metabolites ◽  
Total Phenolic ◽  
Anticancer Activities ◽  
Antioxidant Power ◽  
Dry Extract

The in vitro biological activities of the ethyl acetate extract of the culture filtrate from Streptomyces achromogenes TCH4 (TCH4 extract) were evaluated. The ethyl acetate extract of TCH4 produced a bacteriostatic effect against Enterobacter cloacae, Staphylococcus aureus, Staphylococcus saprophyticus, Bacillus subtilis, methicillin-resistant S. aureus (MRSA) and Klebsiella pneumoniae. The extract had bactericidal activity against S. aureus, S. saprophyticus, S. aureus (MRSA) and K. pneumoniae with minimum bactericidal concentration (MBC) values in the range of 500-1000 ?g/mL. The total phenolic and flavonoid contents in TCH4 were 107.20?2.57 mg gallic acid equivalent (GAE)/g and 44.91?0.84 mg quercetin equivalent (QE)/g of dry extract. Antioxidant activity was determined by DPPH radical (IC50 299.64?6.83 ?g/mL) and ABTS radical scavenging (IC50 65.53?0.95 ?g/mL), and the ferric-reducing antioxidant power (FRAP) (822.76?9.12 mM FeSO4.7H2O/g dry extract) assays. TCH4 exhibited cytotoxic activity in the DU-145 cell line (IC50 9.36?0.37 ?g/mL). Analysis of extract constituents by GC-MS revealed pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) (36.85%), benzeneacetamide (23.76%), and deferoxamine (12.85%) as the major compounds, which have been reported to possess pharmaceutical properties. S. achromogenes TCH4 could be a potential source of bioactive metabolites with antibacterial, antioxidant and anticancer activities for pharmaceutical applications.

scholarly journals Cytotoxicity Activity and Phytochemical Screening of Anthocleista djalonensis Root Extracts against Cancer Cells

2019 ◽  
pp. 1-7 ◽  
Author(s):  
Ijeoma Solomon Okoro ◽  
Terrumum Amom Tor-Anyiin ◽  
John Ogbaji Igoli ◽  
Muluh Emmanuel Khan
Keyword(s):  
Ethyl Acetate ◽  
Hela Cells ◽  
Screening Methods ◽  
Anticancer Activities ◽  
Medicinal Uses ◽  
Cytotoxicity Activity ◽  
Anti Cancer ◽  
Pure Compounds ◽  
Acetone Extracts

Aim: Several medicinal uses have been reported for Anthocleista djalonensis and many types of pure compounds have been isolated. However, the anti-cancer activity of this plant has not been proven. The aim of this study was to screen for the phytochemicals present in the rootn-hexane, ethyl acetate, and acetone extracts of Anthocleista djalonensis, and to evaluate its anticancer potential against human cervix adenocarcinoma cells (HeLa cells) in vitro. Place and Duration of Study: The study was carried out in Department of Organic Chemistry, Rhodes University, Grahamstown, South Africa. The duration period was between March and July, 2016. Methodology: Extracts were prepared by soaking the root powder in the respective solvents with continuous stirring; The extracts were filtered and evaporated to remove the solvents. The extracts were then screened for phytocompounds by preliminary screening methods. Anti-cancer potential was carried out by a Resazurin assay and CC50 values were determined. Results: The extracts showed the presence of carbohydrates, glucoside, alkaloids, flavonoids, terpenoids, tannins, saponins, sterols. All extracts demonstrated moderate cytotoxicity against HeLa cells. Conclusion: The hexane, ethyl acetate and acetone extracts showed anticancer property. The roots extracts of Anthocleista djalonesis were thus found to possess potential anticancer activities.

scholarly journals Semecarpus anacardium Linn. leaf extract exhibits activities against breast cancer and prolongs the survival of tumor bearing mice

2020 ◽  
Author(s):  
Rajesh Kumar Singh ◽  
Amit Ranjan ◽  
Ruchita Tripathi ◽  
Sumit Singh Verma ◽  
Vinamra Sharma ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Dependent Manner ◽  
Microbial Infection ◽  
Mice Model ◽  
Anticancer Activities ◽  
Semecarpus Anacardium ◽  
Tumor Bearing

ABSTRACTSemecarpus anacardium Linn. is a commonly used Ayurvedic medicinal plant which nuts have been described in Ayurveda and Sidda system of medicine to treat clinical ailments such as vitiligo, inflamation, microbial infection, geriatric problem, baldness and neuro related problems. In this study, anti-cancer activity of the leaves of Semecarpus anacardium Linn was evaluated for future drug development. The phytochemical screening was done by GC-MS analysis, cytotoxicity was examined using MTT assay, mode of cell death was evaluated by fluorescence microscopy and finally antitumor activity was determined in EAC cell induced tumor bearing mice. The ethyl acetate extract from the leaves of the plant induced cytotoxicity in cancer cells in a dose dependent manner (IC50: 0.57 μg/ml in MCF-7 cells) in different cancer cell lines. The non-malignant cells were relatively insensitive to the extract. The staining with acridine orange, ethidium bromide and DAPI confirmed that the extract induced apoptosis in cancer cells. Furthermore, the extract induced cell cycle arrest at G1 phase and suppressed cancer cell migration. An oral administration of the extract suppressed the tumor growth in mice model bearing ehrlich ascites carcinoma cells. The ethyl acetate extract was also found to prolong the survival of tumor bearing mice.Overall, these observations suggest the anticancer activities of the ethyl acetate extract of the leaves of S. anacardium. The study opens a new window to examine the phytochemical constituents from the leaves of the plant responsible for the anticancer activities.

GC-analysis, and Antioxidant, Anti-inflammatory, and Anticancer Activities of Some Extracts and Fractions of Linum usitatissimum

2020 ◽  
Vol 16 (9) ◽  
pp. 1306-1318
Author(s):  
Eman A. Morsi ◽  
Hend O. Ahmed ◽  
Heba Abdel-Hady ◽  
Mortada El-Sayed ◽  
Mohamed A. Shemis
Keyword(s):  
Ethyl Acetate ◽  
Anticancer Activity ◽  
Cell Line ◽  
Linum Usitatissimum ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Anticancer Activities ◽  
Anti Inflammatory ◽  
Phenolic And Flavonoid

Context: Linum usitatissimum (Linn); known as Flaxseed, is one of the most important medicinal plants traditionally used for health benefits and also as nutritional purposes. Objective: Estimation of total phenolic and flavonoid contents, and evaluation of the antioxidant, antiinflammatory and anticancer activities have been performed on hexane, ethyl acetate, n-butanol, and methanol extracts and also on fractions of methanol extract (hexane, ethyl acetate, and n-butanol). Materials and Methods: Phenolic and flavonoid contents were detected using spectrophotometric and colorimetric assays. Antioxidant and anti-inflammatory activities were estimated in-vitro. Anticancer activity of extracts was tested on Hepatocellular carcinoma cell line (HepG2) and breast cancer cell line (MCF7). Results: The methanolic extract and its ethyl acetate fraction showed higher contents of total phenols and flavonoids. Also, the methanolic extract showed a higher antioxidant activity. The butanolic and ethyl acetate fractions at concentration 500μg/ml yielded a higher percent of inhibition of protein denaturation; 87.9% and 90%, respectively. The ethyl acetate fraction and the methanolic extract showed an obvious anticancer activity against HepG2 and MCF7 (IC50=60±0.24 and 29.4±0.12μg. ml-1) and (IC50=94.7±0.21 and 227±0.48μg. ml-1), respectively. The GC-MS analysis showed that the methanolic extract had 32 compounds whereas the ethyl acetate and butanol fractions contained 40 and 36 compounds, respectively. Conclusion: Flaxseed contains different biologically active compounds that have been found to possess various activities, which can protect the human body against several diseases.

scholarly journals Ethyl Acetate Extracts of Two Artemisia Species: Analyses of Phenolic Profile and Anticancer Activities Against SW-480 Colon Cancer Cells

2019 ◽  
Vol 14 (5) ◽  
pp. 1934578X1984301
Author(s):  
Marina Radović Jakovljević ◽  
Darko Grujičić ◽  
Marko Živanović ◽  
Milan Stanković ◽  
Andrija Ćirić ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Ethyl Acetate ◽  
Cancer Cells ◽  
Early Stage ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Anticancer Activities ◽  
Human Colon Cancer ◽  
Phenolic Profile ◽  
Ethyl Acetate Extracts

Because Artemisia vulgaris L. and Artemisia alba Turra are traditional medicinal plants used for the treatment of different diseases, we evaluated the cytotoxic/apoptotic activity of ethyl acetate extracts from these natural products against human colon cancer cells SW-480. The extracts contained a large amount of the total polyphenols and flavonoids. The phenolic profile showed the presence of phenolic acids (gallic, p-coumaric, vanillic, and ferulic acids) and flavonoids (rutin, myricetin, luteolin, quercetin, and apigenin). 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay indicated that antiproliferative activities of both A. vulgaris and A. alba extracts increased with the extension of time exposure, with decreasing IC50 values. Mitomycin C (MMC) alone had antiproliferative activity, but in combination with plant extracts caused stronger effect with lower IC50 values. Flow cytometry analyses showed that A. alba extract induced higher percentage of SW-480 cells in the early stage of apoptosis (33.5 ± 1.6 vs 0.7 ± 0.1, P < 0.05), whereas the A. vulgaris extract significantly increased the percentage of cells in necrosis (82.4 ± 5.0 vs 53.9 ± 2.3, P < 0.05). In conclusion, A. alba extract can be considered a potential source of bioactive components with anticancer activity or be used as a dietary food supplement or supplement to chemotherapy due to its synergistic effect with the MMC.

scholarly journals Synthesis and Biological Evaluation of Substituted Thiophenyl Derivatives of Indane-1, 3-Dione

2007 ◽  
Vol 4 (3) ◽  
pp. 428-433 ◽  
Author(s):  
D. Giles ◽  
M. S. Prakash ◽  
K. V. Ramseshu
Keyword(s):  
Ethyl Acetate ◽  
Sulphuric Acid ◽  
Sodium Salt ◽  
Sodium Ethoxide ◽  
Biological Evaluation ◽  
Diethyl Phthalate ◽  
Anticancer Activities ◽  
Ester Derivative ◽  
Synthesis And Biological Evaluation ◽  
Derivatives Of

Indane-1, 3-dione (1) synthesized by condensation of diethyl phthalate and ethyl acetate in presence of sodium ethoxide gave sodium salt of ester derivative, which on neutralization in presence of sulphuric acid afford1. Various thiols where converted to their respective disulphide (3) which on treatment with1gave 2-substituted thio phenyl derivatives (4a-g) of1. The synthesized compounds were characterized on the basis of I.R and H1NMR. Synthesized compounds (4a-g) were investigated for their anticoagulant, analgesic, anti-inflammatory, antifungal, antibacterial and anticancer activities. Some of the synthesized compounds have shown moderate activities.

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