Multimodality Treatment of 15 Malignant WHO III (2007 Criteria) Meningiomas: Differential Survival Rates Between De Novo and Progressive Origins

This study focuses on the incidence, treatment, and survival of de novo acute leukemia in a 25-year perspective in western Sweden and Estonia. At the beginning of our study, Estonia was a part of the Eastern bloc with planned economy, but since 1991 it is a member of the European Union and transforming into a market economy. Survival rates have steadily increased in both countries. However, a gap between their survival curves remains. Based on our data, it is difficult to explain the big difference in the 5-year relative survival in favor of western Sweden (55 vs. 22%). In Germany, there was a big difference in overall cancer survival between East and West Germany after the fall of the iron curtain, but today no difference is seen. Differences in survival are probably due to a higher proportion of intense chemotherapy regimens and a higher rate of hematopoietic stem cell transplantations in Sweden. Other important factors might be better supportive care and diagnostics as well as better adjuvant therapy. Better staff training and conditions in wards are also factors that might play an essential role.

Download Full-text

Prognostic significance of surface marker expression on blasts of patients with de novo acute myeloblastic leukemia.

Journal of Clinical Oncology ◽

10.1200/jco.1990.8.3.423 ◽

1990 ◽

Vol 8 (3) ◽

pp. 423-430 ◽

Cited By ~ 66

Author(s):

I Schwarzinger ◽

P Valent ◽

U Köller ◽

C Marosi ◽

B Schneider ◽

...

Keyword(s):

Complete Remission ◽

De Novo ◽

Surface Membrane ◽

Survival Rates ◽

Prognostic Significance ◽

Acute Myeloblastic Leukemia ◽

Probability Of Survival ◽

Surface Marker Expression ◽

Median Period ◽

De Novo Aml

The prognostic significance of the expression of surface membrane antigens on the blasts of 123 consecutive patients with de novo acute myeloblastic leukemia (AML) was evaluated. For this purpose, reactivity of monoclonal antibodies (mAbs) CLB-ERY3 (antiblood-group H antigen), VIM-D5 (CD15), WT1 (CD7), MY7 (CD13), MY9 (CD33), VID-1 (antihuman leukocyte antigen locus DR [anti-HLA DR]), VIM-2 (CDw65L), VIM-13 (CD14), 63D3 (CD14) and anti-TdT with leukemic blast cell populations was prospectively analyzed with respect to the rates of complete remission (CR), continuous complete remission (CCR), and survival. The overall rate of CR was 65%, the 6-year rates of overall CCR and survival were 23% and 13%, respectively (median period of patient observation, 30 months). Of all Abs tested, four (CLB-ERY3, MY7, anti-TdT, and VIM-D5) were found to be of prognostic value. Reactivity of CLB-ERY3, MY7, and anti-TdT was predictive for CR (CLB-ERY3+, 43% v CLB-ERY3-, 73%, P less than .02; MY7+, 59% v MY7-, 91%, P less than .003; TdT+, 28% v TdT-, 71%, P less than .001, respectively) and probability of survival (significantly lower survival rates: CLB-ERY3+, P less than .02; MY7+, P less than .03; and TdT+ cases, P less than .001, respectively). Reactivity of VIM-D5 was significantly associated with a higher probability of CCR (P less than .01). Our results confirm earlier reports on the prognostic significance of expression of CD13 and TdT in AML and indicate CLB-ERY3 (antiblood-group H antibody) and VIM-D5 (CD15) as further markers predictive for the clinical outcome in patients with de novo AML.

Download Full-text

Antibody-mediated rejection with detection of de novo donor-specific anti-human leucocyte antigen Class II antibodies 3 years after heart transplantation: a case report

European Heart Journal - Case Reports ◽

10.1093/ehjcr/ytz246 ◽

2020 ◽

Vol 4 (1) ◽

pp. 1-4

Author(s):

Bernd Ludwig ◽

Johanna Schneider ◽

Daniela Föll ◽

Qian Zhou

Keyword(s):

Heart Transplantation ◽

De Novo ◽

Survival Rates ◽

Graft Function ◽

Left Ventricular ◽

Cardiac Allograft ◽

Left Ventricular Ejection ◽

High Dose ◽

Ventricular Ejection Fraction ◽

Antibody Mediated Rejection

Abstract Background Antibody-mediated rejection (AMR) in cardiac transplantation may manifest early within the first weeks after transplantation but also late after months to years following transplantation resulting in mild heart failure to cardiogenic shock. While patients with early cardiac AMR are less affected and seem to have survival rates comparable to transplant recipients without AMR, late cardiac AMR is frequently associated with graft dysfunction, fulminant forms of cardiac allograft vasculopathy, and a high mortality rate. Nevertheless, AMR of cardiac allografts remains difficult to diagnose and to treat. Case summary We report the case of a 47-year-old male patient with late AMR of the cardiac allograft 3 years after heart transplantation. Antibody-mediated rejection was confirmed by endomyocardial biopsy and the presence of donor-specific antibodies (DSA). The patient was treated with high dose of prednisolone, plasmapheresis, intravenous Gamma Globulin, rituximab, immunoadsorption, and bortezomib. Under this treatment regimen left ventricular ejection fraction and pro B-type natriuretic peptide recovered, and the patient improved to New York Heart Association Class I. Currently, 3 years after the diagnosis of cardiac AMR, graft function continues to be nearly normal, and there is no evidence for transplant vasculopathy. Discussion This case illustrates that AMR can occur at any time after transplantation. Although graft function fully recovered after treatment in our patient, the level of DSA remained high, suggesting that DSA may not be a reliable parameter to determine the intensity and duration of the therapy.

Download Full-text

RNA-Seq and differential gene expression analysis in Temora stylifera copepod females with contrasting non-feeding nauplii survival rates: an environmental transcriptomics study

BMC Genomics ◽

10.1186/s12864-020-07112-w ◽

2020 ◽

Vol 21 (1) ◽

Cited By ~ 2

Author(s):

Ennio Russo ◽

Chiara Lauritano ◽

Giuliana d’Ippolito ◽

Angelo Fontana ◽

Diana Sarno ◽

...

Keyword(s):

Differential Expression ◽

De Novo ◽

Transcriptome Assembly ◽

Survival Rates ◽

Natural Populations ◽

Zooplankton Community ◽

Differentially Expressed ◽

Diatom Cell ◽

Functional Interpretation ◽

Protein Ubiquitination

Abstract Background Copepods are fundamental components of pelagic food webs, but reports on how molecular responses link to reproductive success in natural populations are still scarce. We present a de novo transcriptome assembly and differential expression (DE) analysis in Temora stylifera females collected in the Gulf of Naples, Mediterranean Sea, where this copepod dominates the zooplankton community. High-Throughput RNA-Sequencing and DE analysis were performed from adult females collected on consecutive weeks (May 23rd and 30th 2017), because opposite naupliar survival rates were observed. We aimed at detecting key genes that may have influenced copepod reproductive potential in natural populations and whose expression was potentially affected by phytoplankton-derived oxylipins, lipoxygenase-derived products strongly impacting copepod naupliar survival. Results On the two sampling dates, temperature, salinity, pH and oxygen remained stable, while variations in phytoplankton cell concentration, oxylipin concentration and oxylipin-per-diatom-cell production were observed. T. stylifera naupliar survival was 25% on May 23rd and 93% on May 30th. De novo assembly generated 268,665 transcripts (isoforms) and 120,749 unique ‘Trinity predicted genes’ (unigenes), of which 50% were functionally annotated. Out of the 331 transcript isoforms differentially expressed between the two sampling dates, 119 sequences were functionally annotated (58 up- and 61 down-regulated). Among predicted genes (unigenes), 144 sequences were differentially expressed and 31 (6 up-regulated and 25 down-regulated) were functionally annotated. Most of the significantly down-regulated unigenes and isoforms were A5 Putative Odorant Binding Protein (Obp). Other differentially expressed sequences (isoforms and unigenes) related to developmental metabolic processes, protein ubiquitination, response to stress, oxidation-reduction reactions and hydrolase activities. DE analysis was validated through Real Time-quantitative PCR of 9 unigenes and 3 isoforms. Conclusions Differential expression of sequences involved in signal detection and transduction, cell differentiation and development offered a functional interpretation to the maternally-mediated low naupliar survival rates observed in samples collected on May 23rd. Down-regulation of A5 Obp along with higher quantities of oxylipins-per-litre and oxylipins-per-diatom-cell observed on May 23rd could suggest oxylipin-mediated impairment of naupliar survival in natural populations of T. stylifera. Our results may help identify biomarker genes explaining variations in copepod reproductive responses at a molecular level.

Download Full-text

Clinical relevance of P-glycoprotein expression in de novo acute myeloid leukemia

Blood ◽

10.1182/blood.v87.5.1997.1997 ◽

1996 ◽

Vol 87 (5) ◽

pp. 1997-2004 ◽

Cited By ~ 89

Author(s):

G Del Poeta ◽

R Stasi ◽

G Aronica ◽

A Venditti ◽

MC Cox ◽

...

Keyword(s):

Flow Cytometry ◽

Acute Myeloid Leukemia ◽

Myeloid Leukemia ◽

De Novo ◽

Survival Rates ◽

Disease Free Survival ◽

Normal Karyotype ◽

Free Survival ◽

Negative Phenotype ◽

Acute Myeloid

Abstract Cytofluorimetric detection of the multidrug resistance (MDR)-associated membrane protein (P-170) was performed at the time of diagnosis in 158 patients with acute myeloid leukemia using the C219 monoclonal antibody (MoAb). In 108 of these cases the JSB1 MoAb was also tested. An improved histogram subtraction analysis, based on curve fitting and statistical test was applied to distinguish antigen-positive from antigen-negative cells. A marker was considered positive when more than 20% of the cells were stained. At onset, P-170 was detected in 43% of cases with C219 and in 73% of cases with JSB1. There was a strict correlation between C219 and JSB1 positivity, as all C219+ cases were also positive for JSB1 MoAb (P < .001). No relationship was found between sex, age, organomegaly, and MDR phenotype. Significant correlation was found between CD7 and both C219 and JSB1 expression (P < .001 and .001, respectively). C219-negative phenotype was more often associated with a normal karyotype (24 of 55 with P = .030). Rhodamine 123 (Rh123) staining and flow cytometry analysis showed a significantly decreased mean fluorescence in 51 C219+ and 38 JSB1+ patients compared to 42 MDR negative ones (P < .001). The rate of first complete remission (CR) differed both between C219+ and C219- cases and between JSB+ and JSB- ones (30.9% v 71.1% and 35.4% v 93.1%, respectively, P < .001). Of the 21 C219+ patients who had yielded a first CR, 19 (90.4%) relapsed, compared with 28 of 64 (43.7%) C219- patients (P < .001). Of the 28 JSB1+ patients in first CR, 17 (60.7%) relapsed relative to 8 (29.6%) of 27 JSBI- ones (P = .021). A higher rate of relapses among MDR+ compared with MDR- patients was observed both for C219 and JSB1 MoAbs taken separately (C219 80% v 44%; JSB1 52% v 27%), with no relationship to age. The survival rates (Kaplan-Meyer method) were significantly shorter both in C219+ patients and in JSB1+ cases (P < .001). Disease-free survival curves followed this same trend. The combination (C219- JSB1+) identified a subset of patients with an intermediate outcome compared to C219 positive cases. The prognostic value of both markers (C219 and JSB1) was confirmed in multivariate analysis. These results suggest that the assessment of MDR phenotype by flow cytometry may be an important predictor of treatment outcome.

Download Full-text

Winter Survival and Territory Acquisition in a Northern Population of Black-Capped Chickadees

The Auk ◽

10.1093/auk/105.4.727 ◽

1988 ◽

Vol 105 (4) ◽

pp. 727-736 ◽

Cited By ~ 59

Author(s):

André Desrochers ◽

Susan J. Hannon ◽

Kelly E. Nordin

Keyword(s):

Survival Rates ◽

Early Spring ◽

Control Area ◽

Winter Survival ◽

Food Supplementation ◽

First Year ◽

Differential Survival ◽

Northern Population ◽

Parus Atricapillus ◽

Lower Ranking

Abstract We assessed the effects of winter food supplementation on differential survival between sex, age and dominance classes, and the effects of feeding and territorial defense on breeding density in a northern population of Black-capped Chickadees (Parus atricapillus). Survival rates were higher in a food-supplemented area than in a control area, which suggests that food abundance limited winter survival. Survival was greater among males than lower-ranking females in 1 of 2 years, and greater in dominants than in subordinates, within sex and age classes. Survival of adults and first-year birds, however, did not differ significantly. In the 2 years of this study, a sharp decline of population size occurred at the onset of territoriality in spring. Birds that disappeared in spring were mainly subordinates of each sex. Breeding densities in control and feeder areas were similar in the two years. Eight of 14 territorial birds removed in 2 years were replaced, implying that a nonbreeding surplus was present in early spring.

Download Full-text

Multimodality Treatment for Esophageal Malignancy: The Roles of Surgery and Neoadjuvant Therapy

The American Surgeon ◽

10.1177/000313480907500607 ◽

2009 ◽

Vol 75 (6) ◽

pp. 489-497 ◽

Cited By ~ 3

Author(s):

Edward Malin ◽

Paul D. Kiernan ◽

Michael J. Sheridan ◽

Sandeep J. Khandhar ◽

Cheryl Fraser ◽

...

Keyword(s):

Hospital Mortality ◽

Performance Status ◽

Single Agent ◽

Survival Rates ◽

Poor Performance ◽

Multimodality Treatment ◽

Stage I ◽

Poor Performance Status ◽

Esophageal Malignancy

The best curative treatment for esophageal malignancy remains controversial. In 2003, we presented our institution's experience with 124 patients treated from 1990 to 2001. Here we update that experience with an additional 6 years’ data. A total of 221 patients underwent surgical resection from 1990 to 2007; 128 had up-front surgery, 88 underwent surgery after neoadjuvant radiation and chemotherapy (NARCS), and five underwent surgery after neoadjuvant, single-agent therapy Principle outcomes of interest were 30-day and in-hospital mortality as well 3- and 5-year survival rates. Overall 3- and 5-year survival rates were 38 and 33 per cent. NARCS achieved complete pathologic result in 32 per cent of patients with corresponding 3- and 5-year survival rates of 58 and 53 per cent. The 3- and 5-year survival rates for all patients undergoing NARCS were 36 and 31 per cent versus 24 and 18 per cent for patients with up-front surgery for anything over Stage I disease ( P = 0.01). The 3- and 5-year survival rates for patients with up-front resection of Stage I disease were 78 and 70 per cent. Overall, 30-day and in-hospital mortalities were 1.8 and 2.3 per cent. Since January 1, 2000, hospital mortality has been less than 0.8 per cent. We prefer NARCS for malignancy of the esophagus, except in those patients with high-grade dysplasia (carcinoma in situ), suspected Stage I disease, poor performance status, or urgent/emergent circumstances.

Download Full-text

Expression of the multidrug resistance-associated P-glycoprotein (P- 170) in 59 cases of de novo acute lymphoblastic leukemia: prognostic implications [see comments]

Blood ◽

10.1182/blood.v81.9.2394.2394 ◽

1993 ◽

Vol 81 (9) ◽

pp. 2394-2398 ◽

Cited By ~ 160

Author(s):

JE Goasguen ◽

JM Dossot ◽

O Fardel ◽

F Le Mee ◽

E Le Gall ◽

...

Keyword(s):

Multidrug Resistance ◽

Acute Lymphoblastic Leukemia ◽

Complete Remission ◽

De Novo ◽

Lymphoblastic Leukemia ◽

Survival Rates ◽

Glycoprotein P ◽

Kaplan Meier ◽

Prognostic Implications ◽

First Complete Remission

Abstract Immunocytochemical detection of the multidrug resistance (MDR)- associated membrane protein (P-170) was performed at time of diagnosis in a series of 36 children and 23 adults with acute lymphoblastic leukemia (ALL) using two monoclonal antibodies JSB1 and C219. Immunophenotypes were obtained in all cases and karyotypes were analyzed in 37 cases. Detection with JSB1 or with C219 led to similar results in terms of positive cells and cases, but the intensity of staining was higher with JSB1. In the populations studied, the rate of first complete remission differed between MDR-positive and MDR-negative in adult patients only (56% v 93%, respectively, P = .05). Of the 16 MDR-positive patients who had presented a first complete remission, 13 (81%) relapsed, compared with 13 of 35 (37%) MDR-negative (P = .008) patients. A higher rate of relapse among MDR-positive compared with MDR- negative patients was observed in adults and in children taken separately (adults 100% v 46%; children 73% v 32%, respectively). The survival rates (Kaplan-Meier method) were significantly higher in MDR- negative compared with MDR-positive populations as a whole (P = .002) and among children (P = .05) and adults (P = .03) taken separately. Event-free survival curves followed this trend. The percentage of second complete remission was very low in the MDR-positive group (15%) compared with 38% for the MDR-negative group. These results were shown by multivariate analysis to be independent of age, immunophenotypes, and karyotypes and clearly show the importance of MDR phenotype detection in ALL.

Download Full-text

MDR1 gene–related clonal selection and P-glycoprotein function and expression in relapsed or refractory acute myeloid leukemia

Blood ◽

10.1182/blood.v97.11.3605 ◽

2001 ◽

Vol 97 (11) ◽

pp. 3605-3611 ◽

Cited By ~ 51

Author(s):

Marry M. van den Heuvel-Eibrink ◽

Erik A. C. Wiemer ◽

Marjan J. de Boevere ◽

Bronno van der Holt ◽

Paula J. M. Vossebeld ◽

...

Keyword(s):

Acute Myeloid Leukemia ◽

Genetic Polymorphism ◽

Myeloid Leukemia ◽

De Novo ◽

Clonal Selection ◽

Survival Rates ◽

Mdr1 Gene ◽

Allelic Variants ◽

P Glycoprotein ◽

Acute Myeloid

The expression of P-glycoprotein (P-gp), encoded by theMDR1 gene, is an independent adverse prognostic factor for response and survival in de novo acute myeloid leukemia (AML). Little is known about MDR1 expression during the development of disease. The present study investigated whether MDR1 gene– related clonal selection occurs in the development from diagnosis to relapsed AML, using a genetic polymorphism of the MDR1 gene at position 2677. Expression and function of P-gp were studied using monoclonal antibodies MRK16 and UIC2 and the Rhodamine 123 retention assay with or without PSC 833. No difference was found in the levels of P-gp function and expression between diagnosis and relapse in purified paired blast samples from 30 patients with AML. Thirteen patients were homozygous for the genetic polymorphism ofMDR1 (n = 7 for guanine, n = 6 for thymidine), whereas 17 patients were heterozygous (GT). In the heterozygous patients, no selective loss of one allele was observed at relapse. Homozygosity for the MDR1 gene (GG or TT) was associated with shorter relapse-free intervals (P = .002) and poor survival rates (P = .02), compared with heterozygous patients. No difference was found in P-gp expression or function in patients with AML with either of the allelic variants of the MDR1 gene. It was concluded that P-gp function or expression is not upregulated at relapse/refractory disease and expression of one of the allelic variants is not associated with altered P-gp expression or function in AML, consistent with the fact that MDR1 gene–related clonal selection does not occur when AML evolves to recurrent disease.

Download Full-text