The Increase of the Electrophoretic Mobility of Platelets after Laparotomy

1980 ◽  
Vol 44 (01) ◽  
pp. 043-045 ◽  
Author(s):  
Hiroh Yamazaki ◽  
Ryoko Tsukui ◽  
Takeshi Motomiya ◽  
Stephanie May Jung ◽  
Minoru Sonoda ◽  
...  
Keyword(s):  
Electrophoretic Mobility ◽  
Colloidal Particles ◽  
Surgical Stress ◽  
Human Platelets ◽  
Automatic Instrument ◽  
Fully Automatic ◽  
Males And Females ◽  
The Difference ◽  
Plug Formation

SummaryA fully automatic instrument for the determination of electrophoretic mobility of colloidal particles was applied to human platelets. A significant increase in platelet electrophoretic mobility was observed one day after a laparotomy. This suggests that a selective consumption of platelets with smaller surface negative charge may occur during postoperative hemostatic plug formation or under surgical stress. In addition, the difference in electrophoretic mobility observed between males and females suggests an effect of estrogen on platelets.

scholarly journals Amplitude response of a telemetered seismic system from seismometers to digital acquisition system

1995 ◽  
Vol 38 (1) ◽  
Author(s):  
R. Di Giovambattista ◽  
S. Barba ◽  
A. Marchetti
Keyword(s):  
Digital Signals ◽  
Amplitude Response ◽  
Automatic Mode ◽  
Steady State Method ◽  
Sinusoidal Current ◽  
Fully Automatic ◽  
The Difference ◽  
High Degree ◽  
Seismic System

Automated amplitude response of the complete seismometer, telemetry and recording system js obtaiued trom sinusoidal inputs to the calibration coil. Custom-built software was designed to perform fully automatic cali- bration analyses of the digital signals. In this paper we describe the signals used for calibration and interactive and batch procedures designed to obtain calibration functions in automatic mode. By using a steady-state method we reach a high degree of accuracy in the determination of both the frequency and amplitude of the \ignal. The only parameters required by this procedure are the seismometer mass, the calibration-coil constant and the intensity of the current injected into the calibration coil. This procedure is applicable to telemetered seismic systems and represents an optimization of the processing time. The software was designed to requjre no modification" jf the device used to generate the sinusoidal current should change. In particular, it is possi- ble to changc the number of monotrequcncy packages transmitted to the calibration coil with the on]y restric- tion that the difference between the frequency of two consecutjve packages be greater than 5%; for these rea- sons the procedure is expected to be usefu] for the seismological community. The paper inc]udes a generaI de- scription of thc designing criteria, and of the hardware and software architecture, as well as an account of thc system's performancc during a two year period of operation.

scholarly journals Establishment of a new relationship between posed smile width and lower facial height: A cross-sectional study

2015 ◽  
Vol 09 (03) ◽  
pp. 394-399 ◽  
Author(s):  
Aby Abraham ◽  
Jinu George ◽  
Elbe Peter ◽  
Koshi Philip ◽  
Rajesh Chankramath ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Facial Height ◽  
The Face ◽  
Facial Proportions ◽  
Males And Females ◽  
The Mean ◽  
The Difference

ABSTRACT Objective: The present study is intended to add a new parameter that would be useful in orthodontic clinical evaluation, treatment planning, and determination of vertical dimension (at occlusion). Materials and Methods: Standardized videographic recording of 79 subjects during posed smile was captured. Each video was then cut into 30 photos using the free studio software. The widest commissure-to-commissure posed smile frame (posed smile width [SW]) was selected as one of 10 or more frames showing an identical smile. Lower third of the face is measured from subnasale to soft tissue menton using a digital vernier caliper. Two values were then compared. Ratio between lower facial height and posed SW was calculated. Results: The co-relation between smiling width and lower facial height was found to be statistically significant (P < 0.01). The ratio of lower facial height and smiling width was calculated as 1.0016 with a standard deviation (SD) = 0.04 in males and 1.0301 with an SD = 0.07 in females. The difference between the mean lower facial height in males and females was statistically significant with a t = 10.231 and P = 0.000. The difference between the mean smiling width in males and females was also statistically significant with a t = 5.653 and P = 0.000. Conclusion: In class I subjects with pleasing appearance, normal facial proportions, normal overjet and overbite, and average Frankfort mandibular angle, the lower facial height (subnasale to soft tissue menton) is equal to posed SW.

scholarly journals The Haemoglobin Concentration of Normal English Males and Females

1933 ◽  
Vol 33 (1) ◽  
pp. 36-41 ◽  
Author(s):  
C. E. Jenkins ◽  
C. S. D. Don
Keyword(s):  
Statistical Significance ◽  
Haemoglobin Concentration ◽  
Gas Analysis ◽  
Experimental Accuracy ◽  
Males And Females ◽  
The Mean ◽  
The Difference ◽  
Made In

The determination of the mean normal haemoglobin concentration has re ceived more attention in America than in this country. Recently Price-Jones (1931) has compared the values in London with those found in America. He used the Haldane haemoglobinometer and his results are as accurate as is possible having regard to the method employed. In America his results were 3 percent. above those of the Haldane gas analysis apparatus (1901) by which theclinical instrument is supposed to be calibrated. Twenty comparisons were made in America and showed that the Haldane gas apparatus gave results averaging 2·6 per cent. below those of the Van Slyke apparatus. Price-Jones considers this to have no statistical significance. From the standpoint of experimental accuracy the difference is appreciable, as all methods for the measurement of oxygen combined with Hb are essentially pragmatic.

Platelet glycoprotein Ibα polymorphisms modulate the risk for myocardial infarction

2004 ◽  
Vol 92 (08) ◽  
pp. 384-386 ◽  
Author(s):  
Margareth Ozelo ◽  
Andrea Origa ◽  
Francisco Aranha ◽  
Antonio Mansur ◽  
Joyce Annichino-Bizzacchi ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Tandem Repeats ◽  
Human Platelet ◽  
Human Platelets ◽  
Variable Number ◽  
Platelet Glycoprotein ◽  
Platelet Antigen ◽  
Human Platelet Antigen ◽  
Plug Formation

SummaryPlatelet glycoprotein Iba (GPIba) gene polymorphisms have been reported to affect the risk of developing coronary heart disease. Here, within the GPIba gene, we determine the association between the variable number of tandem repeats (VNTR), the -5C/T Kozak sequence dimorphism, and the human platelet antigen (HPA)-2 polymorphisms with occurrence of myocardial infarction (MI). Patients (n=180) presenting survivors of MI were compared to 180 controls matched by age, gender, and race. Carriers of VNTR-CD genotype had a 2-fold higher risk for MI compared to controls. The prevalence of VNTR-BC was lower among patients than among controls (P=.007). These data are in agreement with recent reports of increased plug formation by human platelets containing VNTRCD but no other VNTR genotypes. Among patients, the number of vessels severely occluded was greater among carriers of the D-allele (P=.019) or VNTR-CD (P=.026) and lower among carriers of the C-allele (P=.003) or VNTR-CC (P=.0009) compared to non-carriers of these alleles. No influence was seen with the Kozak or HPA-2 polymorphisms. Determination of VNTR of the GPIba gene may prove useful for identifying high-risk individuals for MI.

scholarly journals The Relationship Among Platelet Age, Volume and Electrophoretic Mobility

1977 ◽  
Author(s):  
T. Motomiya ◽  
H. Yamazaki ◽  
Y. Sawasaki ◽  
O. Nakajima
Keyword(s):  
Electrophoretic Mobility ◽  
Platelet Adhesion ◽  
Negative Charge ◽  
Free Flow ◽  
Cathode Side ◽  
Extreme Right ◽  
The Difference ◽  
The Relationship ◽  
Edta Blood

The platelet carries a net negative charge and its change may be an important factor in platelet adhesion and aggregation. Platelet age and volume are also believed to be important factors in its functions. Six male rabbits were injected with 50 μCi of 75Selenomethionine intravenously and EDTA-blood was drawn 3 days after. Platelets were washed 3 times and suspended in 3 mM triethanolamine acetate carrier buffer. Platelet electrophoresis was performed using with a Continuous Free-Flow Electrophoresis VAP-5 (Bender & Hobein, Munich). Electrode buffer used was 100 mM triethanolamine acetate buffer pH 7.4 and electrophoresis was performed at 17.5°C, 1250V and 80mA. Approximately 5×108 platelets were electrophoresed per hour into 90 fractions. The fraction #1 is the anode side and #90 cathode side. Platelets in each fraction obtained as a suspension in the buffer were studied for platelet count and volume determinations using with a Coulter Counter ZbI and a Channelyzer C-1000. The radioactivity was measured by a well-type scintilation counter. Platelets were separated well making a single peak at the 27±3.4 fraction. The radioactivity of platelet increased as the fraction number increased but platelet in bilateral side fractions was too small in number and determination of the radioactivity was difficult and the peak radioactivity was at the measurable extreme right fraction (68±3.8). The largest platelet volume also appeared at the measurable extreme right (cathode side) fraction. In conclusion, Continuous Free-Flow Electrophoresis separated platelets well by the difference of cell surface potentials. 75Selenomethionine labeled platelets which have been thought to be young seem to have low electrophoretic mobility and relatively large volume.

Assessment of Damage to Human Platelets after Aggregation and Other Injuries by Microscopic Observation and Estimation of Serotonin Uptake

1970 ◽  
Vol 23 (02) ◽  
pp. 261-275 ◽  
Author(s):  
G Zbinden ◽  
J. N Mehrishi ◽  
S Tomlin
Keyword(s):  
Platelet Aggregation ◽  
Electrophoretic Mobility ◽  
Human Platelets ◽  
Freezing And Thawing ◽  
Low Degree ◽  
Microscopic Evaluation ◽  
Negative Surface ◽  
Negative Surface Charge ◽  
Charged Macromolecules ◽  
5 Ht Uptake

SummaryThe severity of platelet damage induced by hyper- and hypotonic NaCl solutions and freezing and thawing was assessed by microscopic evaluation and measuring inhibition of 5-HT uptake. The same techniques were used to quantitate the effects of aggregating agents. The positively charged macromolecules PS, Poly-L und Poly-O reduced the net negative surface charge as determined by microelectrophoresis, caused platelet aggregation and inhibited 5-HT uptake. The damaging effects of Poly-L and Poly-O were more severe and more closely related to concentration than that of PS. The negatively charged macromolecules Poly-IC and NaPS increased the anodic electrophoretic mobility. Poly-IC and heparin caused a low degree of platelet clumping and no inhibition of 5-HT uptake. NaPS produced severe platelet damage with extensive clumping and complete inhibition of 5-HT uptake. Na laurate had the same effect, but did not alter electrophoretic mobility. ADP caused concentration-dependent platelet aggregation and inhibition of 5-HT uptake. The effects of ADP and NaPS were compared in agitated and non-agitated platelet samples containing identical concentrations of the 2 compounds. Agitation was found to increase the degree of platelet clumping and to reduce 5-HT uptake.

Immunological Evidence for Prothrombin in Human Platelets

1986 ◽  
Vol 55 (02) ◽  
pp. 268-270
Author(s):  
R J Alexander
Keyword(s):  
Electrophoretic Mobility ◽  
Platelet Activation ◽  
Double Diffusion ◽  
Human Platelets ◽  
Secreted Proteins ◽  
Platelet Surface ◽  
Activated Platelets ◽  
Similar Protein ◽  
Diffusion Assay ◽  
Supernatant Solution

SummaryAn attempt was made to isolate from plasma the platelet surface substrate for thrombin, glycoprotein V (GPV), because a GPV antigen was reported to be present in plasma (3). Plasma fractionation based on procedures for purification of GPV from platelets revealed a thrombin-sensitive protein with appropriate electrophoretic mobility. The protein was purified; an antiserum against it i) reacted with detergent-solubilized platelet proteins or secreted proteins in a double diffusion assay, ii) adsorbed a protein from the supernatant solution of activated platelets, and iii) inhibited thrombin-induced platelet activation, but the antiserum did not adsorb labeled GPV. The purified protein was immunochemically related to prothrombin rather than to GPV. Other antibodies against prothrombin were also able to adsorb a protein from platelets. It is concluded that 1) plasma does not contain appreciable amounts of GPV, and 2) platelets contain prothrombin or an immunochemically similar protein.

FRACTIONAL DETERMINATIONS OF URINARY 17-KETOSTEROIDS IN HYPEREMESIS GRAVIDARUM

1962 ◽  
Vol 41 (1) ◽  
pp. 123-128 ◽  
Author(s):  
Pentti A. Järvinen ◽  
Sykkö Pesonen ◽  
Pirkko Väänänen
Keyword(s):  
Hyperemesis Gravidarum ◽  
First Trimester ◽  
Control Group ◽  
Before And After ◽  
Daily Urine ◽  
First Trimester Of Pregnancy ◽  
The Difference

ABSTRACT The fractional determination of 17-ketosteroids in the daily urine was performed in nine cases of hyperemesis gravidarum and in four control cases, in the first trimester of pregnancy both before and after corticotrophin administration. The excretion of total 17-KS is similar in the two groups. Only in the hyperemesis group does the excretion of total 17-KS increase significantly after corticotrophin administration. The fractional determination reveals no difference between the two groups of patients with regard to the values of the fractions U (unidentified 17-KS), A (androsterone) and Rest (11-oxygenated 17-KS). The excretion of dehydroepiandrosterone is significantly higher in the hyperemesis group than in the control group. The excretion of androstanolone seems to be lower in the hyperemesis group than in the control group, but the difference is not statistically significant. The differences in the correlation between dehydroepiandrosterone and androstanolone in the two groups is significant. The high excretion of dehydroepiandrosterone and low excretion of androstanolone in cases of hyperemesis gravidarum is a sign of adrenal dysfunction.

AVALIAÇÃO DO CONHECIMENTO TRADICIONAL DOS PESCADORES DA RDSM APLICADO À IDENTIFICAÇÃO DO SEXO DE PIRARUCUS

2010 ◽  
Vol 5 (2) ◽  
pp. 59-66
Author(s):  
Kelven Lopes ◽  
Helder Lima de Queiroz
Keyword(s):  
Sex Determination ◽  
Traditional Knowledge ◽  
Structured Interviews ◽  
Sustainable Fisheries ◽  
Arapaima Gigas ◽  
Males And Females

This study examined the traditional knowledge of fishermen at Mamirauá Reserve about sex determination of pirarucus, Arapaima gigas Schinz 1822. We evaluated the criteria used for by fishermen in the sector Jarauá at Mamirauá Reserve for this determination, during the fisheries season, from October to November 2004. We analyzed responses of a group of about 15 fishermen collected in structured interviews regarding 109 individual pirarucus, 65 males and 44 females. From this sample, only 64 fish have their sex correctly predicted by the fishermen. Although the traditional knowledge of local fishermen is a key component of the sustainable fisheries of this species, this knowledge is not functional in all ranges and aspects, as in sex distinction. We found that the local fishermen evaluated are not able to recognize the sex of pirarucus, although some criteria applied for this purpose are consistent with their biology. The rates of correct forecasts for recognition of males and females were similar to those obtained by chance, even when the criteria applied were consistent, as the criterion “coloration”, which was significantly consistent for identification of males. Yet the group of fishermen interviewed in this study apparently did not apply this criterion correctly.

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