scholarly journals Evaluation of Hydroalcoholic Extract of Convolvulus pluriculis (Shankapushpi) for Standardization by Colorimetric Method: A Preliminary Report

2019 ◽  
Vol 09 (03) ◽  
pp. 121-126
Author(s):  
Gangadhara Swamy ◽  
Rajendra Holla ◽  
Suresh R. Rao
Keyword(s):  
Colorimetric Method ◽  
Herbal Medicines ◽  
Biological Properties ◽  
Biological Action ◽  
Bioactive Substances ◽  
Hydroalcoholic Extract ◽  
High Profile ◽  
Drug Companies ◽  
Routine Monitoring ◽  
Convolvulus Pluricaulis

Abstract Aim This study was aimed to evaluate the hydroalcoholic extract of Convolvulus pluricaulis (HACP) for standardization using colorimetric method. Materials and Methods Establishment of standardization for the Ayurvedic formulations is most important for its chemical compounds, biological action, and its quality reassurance in production and manufacturing of traditional herbal medicines. As most of the drugs are standardized, drug companies are using substitute drugs instead of true drugs. So to make finest superiority drugs, it is necessary to validate the raw drugs. Observing the existing trend in mind, HACP was subjected to standardize procedures for the phytochemical tests. The separation of the bioactive substances from the HACP was performed using both manual methods and high-profile thin-layer chromatography (HPTLC). Results From this study, it is revealed that the seed contains alkaloids, carbohydrates, steroids, tannins, terpenoid, and phenol which gave the medicine numerous therapeutic properties. Conclusion The study was rapid, reproducible, and could be used for routine monitoring of various biological properties of HACP.

scholarly journals A Preliminary Investigation for Standardization of Hydroalcoholic Extract of Mucuna pruriens (Kapikaccu): An Important Drug Used to Treat Parkinson’s Disease in Ayurveda

2019 ◽  
Vol 09 (03) ◽  
pp. 085-090
Author(s):  
Gangadhara Swamy ◽  
Rajendra Holla ◽  
Suresh R. Rao
Keyword(s):  
Preliminary Investigation ◽  
Herbal Medicines ◽  
Biological Action ◽  
Mucuna Pruriens ◽  
Bioactive Substances ◽  
Hydroalcoholic Extract ◽  
High Profile ◽  
Drug Companies ◽  
Manual Methods ◽  
Layer Chromatography

AbstractEstablishment of standardization for the Ayurvedic formulations is most important for its chemical compounds, biological action, and its quality reassurance in production and manufacturing of traditional herbal medicines. As most of the drugs are standardized, drug companies are using substitute drugs instead of true drugs. So, to make finest superiority drugs it is necessary to validate raw drugs. Observing the existing trend in mind, hydroalcoholic extract of Mucuna pruriens seeds (HAMP) was subjected to standardize the procedures for phytochemical tests. The separation of bioactive substances from HAMP was performed using both manual methods and high-profile thin layer chromatography. From the current study, it is revealed that the seed contains alkaloids, steroids, carbohydrates, tannins, flavonoids, and coumarins, which gave the medicine numerous therapeutic properties.

Aktywność biologiczna ekstraktów z propolisu

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Magdalena Woźniak ◽  
Anna Kwiatkowska ◽  
Elżbieta Hołderna-Kędzia ◽  
Katarzyna Sosnowska ◽  
Lucyna Mrówczyńska ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biological Activity ◽  
Phenolic Compounds ◽  
Propylene Glycol ◽  
Ethyl Alcohol ◽  
Antiradical Activity ◽  
Colorimetric Method ◽  
Biological Properties ◽  
Bioactive Substances ◽  
Dpph Test

Introduction. Propolis, also known as bee glue, is a resinous material collected by honeybees with numerous biological properties, including antibacterial, antifungal, antioxidant and anticancer effects. Due to its health-promoting properties, propolis is a component of many products, including dietary supplements, cosmetics and healthy food. Aim. The aim of the study was to determine the antibacterial, antifungal and antioxidant activity of propolis extracts, as well as to compare the biological activity of propolis extracts, depending on the solvent used – ethyl alcohol or propylene glycol. Material and methods. Two propolis extracts were used in the research – the first was prepared in ethyl alcohol, and the second in propylene glycol. The antimicrobial activity of the examined extracts was determined against S. aureus, E. coli and C. albicans. The antioxidant activity was determined on the basis of the evaluation of their antiradical activity in the DPPH· test and Fe2+ chelating activity. Moreover, the total content of phenolic compounds and flavonoids in the tested extracts was determined using the colorimetric method. Results. The tested propolis extracts, regardless of the solvent used (ethyl alcohol or propylene glycol), showed high antibacterial (against S. aureus), antifungal (against C. albicans) and antioxidant (antiradical activity in the DPPH· test and ferrous iron chelating potency in the ferrozine test) activity. Moreover, both tested extracts were characterized by a high and similar content of bioactive compounds – phenolic compounds and flavonoids. Conclusions. The results of the conducted tests showed that the solvent used did not affect determined biological activity and the content of bioactive substances in the tested propolis extracts.

Potential Anti-inflammatory Effects of Hesperidin from the Genus Citrus

2019 ◽  
Vol 25 (37) ◽  
pp. 4929-4945 ◽  
Author(s):  
Silvia Tejada ◽  
Samuel Pinya ◽  
Miquel Martorell ◽  
Xavier Capó ◽  
Josep A. Tur ◽  
...  
Keyword(s):  
Large Family ◽  
Potential Effect ◽  
Biological Properties ◽  
High Concentration ◽  
Specific Mechanism ◽  
Bioactive Substances ◽  
Dietary Polyphenols ◽  
Beneficial Effects ◽  
Anti Inflammatory ◽  
Antioxidant Effects

Background: The benefits of the Mediterranean diet for protecting against many diseases are usually attributed to high consumption of certain foods, characterized by the presence of bioactive substances such as polyphenols. Inflammation plays an important role in the pathogenesis of numerous diseases such as arthritis, allergies or neurodegenerative disorders. Dietary polyphenols constitute a large family of bioactive substances with potential beneficial effects against a broad group of diseases. Citrus fruits and juices are a rich source of vitamin C and flavonoids, with a potential effect on the inflammatory response. Objective: The aim was to evidence the potential anti-inflammatory effects of the flavonoids hesperidin for its possible therapeutic application against diverse pathologies. Method: In the present review, available literature about the anti-inflammatory effects of hesperidin is reported and discussed. Moreover, we also discuss the chemistry, bioavailability and proposed mechanisms of action of hesperidin. Results: Hesperidin is a flavonoid present in high concentration in citrus species and has numerous biological properties, principally antioxidant and anti-inflammatory. Several studies have been performed in order to evaluate the effects of hesperidin as anti-inflammatory agent using cellular and animal models and few clinical trials. Hesperidin treatment decreased inflammatory mediators and exerted significant antioxidant effects. The molecular basis for its anti-inflammatory effects seems to be mediated by signalling pathways especially the nuclear factor κβ pathway. Conclusion: Although hesperidin evidenced anti-inflammatory effects, the specific mechanism of action is not completely known and additional studies are required for elucidation of the molecular targets.

Products of plant origin – benefits and the potential risk for the consumer

2017 ◽  
Vol 86 (1) ◽  
pp. 47
Author(s):  
Monika Karasiewicz ◽  
Anna Bogacz ◽  
Jana Krzysztoszek ◽  
Daniel Pędziwiatr ◽  
Bogusław Czerny
Keyword(s):  
Raw Materials ◽  
Herbal Medicines ◽  
Plant Origin ◽  
Bioactive Substances ◽  
Active Compounds ◽  
Synthetic Drugs ◽  
Diet Supplements ◽  
Phytochemical Compounds ◽  
Higher Doses

Phytochemical compounds are widely used in traditional medicine in the treatment of many ailments. In recent years, an increasing interest is observed in the use of new natural bioactive substances and whole standardized extracts in the prevention and therapy of diseases. Some of these are the components of the diet, diet supplements or at higher doses are used as herbal medicines. Many phytochemicals have documented a beneficial effect on health, but they must be used properly. Therefore, it is important to inform about differentiating between herbal medicine and dietary supplement. Further, the possibility of interactions with synthetic drugs and the mechanisms of these effects is necessary to describe for the safety of phytotherapy. The goal of our paper is to show high prophylactic and medicinal potential of natural active compounds of plant origin. We also want to draw attention to the safety of their use by the consumer. Therefore, we present some studies on the benefical properties of natural active compounds, mainly in the prevention and treatment of cancers and neurodegenerative diseases. The results of the described studies are extensively discussed and their suitability for further testing in vivo and in clinical trials is examined. At the same time we show selected interaction of common medicinal plants or their raw materials with synthetic drugs.

scholarly journals Biological properties of lactobacillus

2003 ◽  
Vol 2 (4) ◽  
pp. 50-58
Author(s):  
N. A. Glushanova
Keyword(s):  
Human Health ◽  
Functional Foods ◽  
Cholesterol Content ◽  
Biological Properties ◽  
Human Organism ◽  
Secondary Immunodeficiency ◽  
Antagonist Activity ◽  
Bioactive Substances ◽  
Causative Agents ◽  
Positive Effect

Lactobacillus are an important component of the human and animal resident microflora. Lactobacillus possess a pronounced antagonist activity with respect to pathogens and conditionally pathogens as well as to other kinds and even to lactobacillus strains. Lactobacillus have immunomodulatory and antineoplastic effects, decrease cholesterol content and synthesize vitamins and other bioactive substances. Along with the positive effect on the human health lactobacillus may be a reason of diseases of people with secondary immunodeficiency. L. casei sp. rhamnosus, L. plantarum, L. brevis, L. lactis, L. fermentum, L. acidophilus and L. salivarius have been registered as causative agents. Hyperlactacidemia syndrome of children during bowels colonization by L. fermentum and L. buchneri has been described. Some L. buchneri strains are able to synthesize histamine that may be a reason of food toxicosis. Lactobacillus strains used as probiotics for correction of resident microflora imbalance as well as in production of functional foods, should not possess the properties, negatively affecting a human organism.

scholarly journals Evaluation of Peroxidase in Herbal Medicines Based on an Electrochemical Sensor

2021 ◽  
Vol 9 ◽  
Author(s):  
Yinzi Yue ◽  
Lianlin Su ◽  
Min Hao ◽  
Wenting Li ◽  
Li Zeng ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Electrochemical Sensor ◽  
Species Identification ◽  
Modified Electrode ◽  
Colorimetric Method ◽  
Herbal Medicines ◽  
Detection Technique ◽  
Plant Tissues ◽  
Electrocatalytic Reduction ◽  
Species Specific

Peroxidases are species-specific. Differences in peroxidase can objectively reflect the genetics among species. The use of peroxidase to assist in species identification is relatively simple and effective. In this work, we proposed a graphene-modified electrode. This electrode can amplify the signal of electrocatalytic reduction of hydrogen peroxide. Since peroxidase can catalyze the reduction of hydrogen peroxide, this signal can be used as an indicator to demonstrate the content of peroxidase in different plant tissues. Twelve herbal medicines were selected for our study. The results show that this electrochemical-based detection technique was comparable to colorimetric method in terms of accuracy.

scholarly journals Consequence of concurrent use of chloroquine and hydroalcoholic extract of Balanites aegyptiaca and leaf leatx of Aloe camperi

2016 ◽  
Vol 5 (1) ◽  
pp. 35-37
Author(s):  
Gereziher Geremedhin Sibhat ◽  
◽  
Mebrahtom Gebrelibanos Hiben ◽  
Keyword(s):  
Herbal Medicines ◽  
Food Plants ◽  
Pharmacokinetic Parameters ◽  
Fruit Extract ◽  
Balanites Aegyptiaca ◽  
Over The Counter ◽  
Plant Materials ◽  
Hydroalcoholic Extract ◽  
Tigray Region ◽  
Concurrent Use

Prescription and/or over the counter medications when taken together with certain foods or herbal substances, leads to either increase or decrease a drug’s therapeutic out comes or adverse effects. It has been reported that a number of plant materials alter some pharmacokinetic parameters of chloroquine when administered concurrently. In some malarious areas of Ethiopia like Tigray region where chloroquine is used as antimalarial drug, medicinal and/or food plants are commonly consumed as herbal medicines or as food items. Thus, this study was aimed to evaluate the potential consequence of oral co-administration of hydroalcoholic fruit extract of Balanites aegyptiaca and leaf latex of Aloe camperi on the antimalarial effectiveness of chloroqine. Extract alone and extract in combination with chloroquine were tested against plasmodium berghie infected mice using peters four day suppressive method. Acute toxicity study was also carried out. The present study revealed that concurrent administrations of leaf extract of Balanites aegyptiaca and leaf leatx of Aloe camperi was found to increase parasitemia suppression potential of chloroquine. From the study it can be concluded that Balanites aegyptiaca and leaf leatx of Aloe camperi can potentiate malaria suppression of chloroquine.

scholarly journals SEA CUCUMBER AS ANTICANCER AGENTS AND ITS DEVELOPMENT FOR FUNCTIONAL FOOD PRODUCTS

2014 ◽  
Vol 9 (2) ◽  
pp. 85 ◽  
Author(s):  
Sherly Ridhowati ◽  
Fransiska Rungkat Zakaria ◽  
Dahrul Syah ◽  
Ekowati Chasanah
Keyword(s):  
Enzymatic Hydrolysis ◽  
Anticancer Agents ◽  
Functional Food ◽  
Sea Cucumber ◽  
Biological Properties ◽  
Bioactive Substances ◽  
Potential Health ◽  
Sea Cucumbers ◽  
Antibacterial And Antifungal

Indonesia is popularly known as sea cucumber (teripang) exporter in the form of dried teripang. Commonly known as beche-de-mer or gamat, sea cucumber has long been used as medicine and food by Asian and Middle East people. Recent study  has shown that sea cucumbers contain active compounds that show potential health benefits and other biological properties such as antibacterial and antifungal products, anticoagulants, antihypertensives, immuno modulation, inhibitor of osteoclastogenesis. It was reported that sea cucumber posses aphrodisiacs, potentially improve immunity, anticancer and anticoagulation. Sea cucumber is also rich in collagen as a component of connective  tissue which can further be converted into smaller molecule and act as bioactive substances. This  review presents  the potential of sea cucumber as a functional food especially to prevent cancer and  strategy to develop sea cucumber-based functional food  by enzymatic hydrolysis and in vivo study.

scholarly journals Screening Papaveraceae as Novel Antibiofilm Natural-Based Agents

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4778
Author(s):  
Sylwia Zielińska ◽  
Magdalena Dziągwa-Becker ◽  
Adam Junka ◽  
Ewelina Piątczak ◽  
Anna Jezierska-Domaradzka ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Biological Properties ◽  
Isoquinoline Alkaloids ◽  
Human Pathogens ◽  
Chelidonium Majus ◽  
Bioactive Substances ◽  
Aerial Parts ◽  
Aconitic Acid ◽  
The Impact

The antimicrobial properties of herbs from Papaveraceae have been used in medicine for centuries. Nevertheless, mutual relationships between the individual bioactive substances contained in these plants remain poorly elucidated. In this work, phytochemical composition of extracts from the aerial and underground parts of five Papaveraceae species (Chelidonium majus L., Corydalis cava (L.) Schweigg. and Körte, C. cheilanthifolia Hemsl., C. pumila (Host) Rchb., and Fumaria vaillantii Loisel.) were examined using LC-ESI-MS/MS with a triple quadrupole analyzer. Large differences in the quality and quantity of all analyzed compounds were observed between species of different genera and also within one genus. Two groups of metabolites predominated in the phytochemical profiles. These were isoquinoline alkaloids and, in smaller amounts, non-phenolic carboxylic acids and phenolic compounds. In aerial and underground parts, 22 and 20 compounds were detected, respectively. These included: seven isoquinoline alkaloids: protopine, allocryptopine, coptisine, berberine, chelidonine, sanguinarine, and chelerythrine; five of their derivatives as well as non-alkaloids: malic acid, trans-aconitic acid, quinic acid, salicylic acid, trans-caffeic acid, p-coumaric acid, chlorogenic acid, quercetin, and kaempferol; and vanillin. The aerial parts were much richer in phenolic compounds regardless of the plant species. Characterized extracts were studied for their antimicrobial potential against planktonic and biofilm-producing cells of S. aureus, P. aeruginosa, and C. albicans. The impact of the extracts on cellular metabolic activity and biofilm biomass production was evaluated. Moreover, the antimicrobial activity of the extracts introduced to the polymeric carrier made of bacterial cellulose was assessed. Extracts of C. cheilanthifolia were found to be the most effective against all tested human pathogens. Multiple regression tests indicated a high antimicrobial impact of quercetin in extracts of aerial parts against planktonic cells of S. aureus, P. aeruginosa, and C. albicans, and no direct correlation between the composition of other bioactive substances and the results of antimicrobial activity were found. Conclusively, further investigations are required to identify the relations between recognized and unrecognized compounds within extracts and their biological properties.

Colorimetric Assay for N-Acetyl-β-D-glucosaminidase (NAG) in Pathological Urine Using the ω-Nitrostyryl Substrate: The Development of a Kit and the Comparison of Manual Procedure with the Automated Fluorimetric Method

1984 ◽  
Vol 21 (4) ◽  
pp. 295-300 ◽  
Author(s):  
C T Yuen ◽  
Patricia R N Kind ◽  
R G Price ◽  
P F G Praill ◽  
A C Richardson
Keyword(s):  
Renal Transplant ◽  
Colorimetric Assay ◽  
Clinical Chemistry ◽  
Colorimetric Method ◽  
Blood Contamination ◽  
Fluorimetric Method ◽  
Transplant Patients ◽  
Routine Monitoring ◽  
Colorimetric Procedure

This paper describes a comparison of the recently developed substrate 2-methoxy-4-(2′-nitrovinyl)-phenyl-2-acetamido-2-deoxy-β-d-glucopyranoside (MNP-GlcNAc) and the corresponding 4-methylumbelliferyl substrate (4-MU-GlcNAc) for the determination of urinary NAG. A good correlation ( r = 0·977) was found between NAG activities in 366 urine samples from renal transplant patients determined by either the fluorimetric method or the colorimetric procedure. The colorimetric method may be used with confidence as an alternative to the fluorimetric assay and does have the advantage that colorimetry is widely used in clinical chemistry laboratories. Sample blanks are not normally required unless there is blood contamination in the urine which can easily be identified visually. The incorporation of the MNP-GlcNAc substrate into a kit suitable for the assay of urinary NAG using a simple battery-operated miniphotometer is also described. The presence of elevated NAG levels in urine can be detected rapidly and this procedure is suitable for routine monitoring of renal patients in the laboratory and may prove to be suitable for use by non-laboratory personnel in clinics or on the ward.

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