Impact of mycotoxins and of a mycotoxin deactivator on alpacas grazing perennial ryegrass infected with wild endophyte (Neotyphodium spp.)

2010 ◽  
Vol 50 (9) ◽  
pp. 902 ◽  
Author(s):  
K. F. M. Reed ◽  
J. L. Vaughan ◽  
L. J. Cummins ◽  
D. D. Moore
Keyword(s):  
Perennial Ryegrass ◽  
Animal Health ◽  
Late Summer ◽  
Treated Group ◽  
The Other ◽  
Control Group ◽  
Liver Enzyme Activity ◽  
Early Autumn ◽  
Lolitrem B ◽  
The Difference

Liveweight gain, animal health and the effectiveness of a mycotoxin deactivator were studied on an old pasture that contained 61% perennial ryegrass. Sixty-seven percent of the ryegrass population was infected with endophyte (Neotyphodium spp.). The pasture was fenced into two halves and two groups of 28 alpaca male weaners were rotated between the two plots. Nine to 10 Suris and 18–19 Huacayas were allocated to each group. One group was fed a concentrate supplement (100 g/head per day) and the other was fed the same supplement to which was added the toxin deactivator, Mycofix® Plus (5 g/100 g). Mean liveweight gain on the low-quality pasture over late summer and early autumn was not significantly (P > 0.05) different between the groups. For the control group it was 41 g/day but individual rates of gain ranged from 67 to 0 g/day, depending on the severity of signs of perennial ryegrass toxicosis (r = 0.82, P < 0.001). Liveweight gain was independent of neurotoxic signs in the Mycofix® Plus treated group. Ergovaline concentration in perennial ryegrass varied from 0.43 to a peak in early autumn (March) of 1.05 mg/kg. Mean urine lysergol alkaloid concentration peaked in mid-summer (January) at 109 ng/mg creatinine (control group) and was consistently lower in the Mycofix® Plus group, although the difference approached significance (P = 0.06) only in March. Lolitrem B concentration in perennial ryegrass varied from 0.78 to 1.57 mg/kg. Neurotoxic signs in alpacas were observed throughout the study and peaked in early autumn, coinciding with peak lolitrem B concentration; at this time, 84% of alpacas exhibited neurotoxic signs. Over the 145-day study, the Mycofix® Plus treated group exhibited a lower mean rating of perennial ryegrass toxicosis signs (P < 0.05). Variation in liveweight gain and signs of toxicosis were not associated with significant differences in liver enzyme activity.

scholarly journals INSIGHTS INTO THE ROLE OF MORUS ALBA IN REVERSING OBESITY-ASSOCIATED HEPATIC STEATOSIS AND RELATED METABOLIC DISORDER IN RATS

2016 ◽  
pp. 231
Author(s):  
Hanaa H. Ahmed ◽  
Fatehya M Metwally ◽  
Hend Rashad ◽  
Asmaa M Zaazaa
Keyword(s):  
Insulin Resistance ◽  
Hepatic Steatosis ◽  
Metabolic Disorder ◽  
Ethanolic Extract ◽  
Treated Group ◽  
Morus Alba ◽  
The Other ◽  
Obese Group ◽  
Control Group

<p>ABSTRACT<br />Objective: The goal of the present study was to examine the viability of Morus alba (M. alba) ethanolic extract in repression of obesity-associated<br />hepatic steatosis and related metabolic disorder; dyslipidemia, hyperinsulinemia, and glycemic status.<br />Methods: Adult female albino rats were randomly assigned into four groups, eight rats each as follows: Group (1) control group received standard<br />rodent diet for 24 weeks. The other three groups administered high cholesterol diet for 12 weeks and served as obese group, M. alba-treated group,<br />and simvastatin-treated group.<br />Results: The current results showed an increment in thoracic circumference (TCX) and abdominal circumferences (AC) as well as body mass index<br />(BMI) in obese group. In addition, dyslipidemia, hyperinsulinemia, hyperglycemia, and insulin resistance have been elucidated in obese group.<br />Moreover, hepatic malondialdehyde (MDA), nitric oxide (NO), serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and bilirubin<br />values were significantly increased in obese groups versus control group. On the other hand, administration of ethanolic extract of Morus alba or<br />simvastatin could significantly lessen BMI and in addition to improve dyslipidemia in obese group. Glucose, insulin levels, and insulin resistance value<br />in serum samples demonstrated a significant reduction in obese group upon treatment with M. alba ethanolic extract or simvastatin. Furthermore,<br />noticeable depletion in hepatic MDA, NO contents, serum ALT, AST activities, and serum bilirubin level was recorded as a result of treatment with<br />either ethanolic extract of M. alba or simvastatin. Histopathological examination of liver tissue showed ballooning degeneration in the hepatocytes<br />(hepatic steatosis) associated with inflammatory cells penetration in portal zone in obese group. Meanwhile, the treatment of obese groups with<br />ethanolic extract of M. alba or simvastatin was found to restore the structural organization of the liver.<br />Conclusion: The present findings provide a novel aspect for understanding of the role of M. alba against obesity-associated liver diseases and related<br />metabolic disorder. The mechanisms underlying these effects seem to depend on the hypolipidemic potential, anti-inflammatory property, and<br />antioxidant activity of its phytochemicals.<br />Keywords: Obesity, Morus alba, Dyslipidemia, Hyperinsulinemia, Hyperglycemia, Hepatic steatosis.</p>

scholarly journals Involvement of the Inconstant Bursa of the Fifth Metatarsophalangeal Joint in Psoriatic Arthritis: A Clinical and Ultrasonographic Study

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Giovanni Ciancio ◽  
Stefania Volpinari ◽  
Maria Fotinidi ◽  
Federica Furini ◽  
Ilaria Farina ◽  
...  
Keyword(s):  
Psoriatic Arthritis ◽  
Healthy Subjects ◽  
Statistical Significance ◽  
Metatarsophalangeal Joint ◽  
Metatarsal Bone ◽  
The Other ◽  
Control Group ◽  
Asymptomatic Patients ◽  
Seronegative Spondyloarthritis ◽  
The Difference

Objective. To evaluate the involvement of the bursa located next to the head of the 5th metatarsal bone in patients with psoriatic arthritis (PsA) in comparison with the other seronegative spondyloarthritis (SpA).Methods. All patients with PsA seen during a period of 24 months were enrolled. The control group included healthy subjects and patients with the other SpA. All subjects underwent clinical and ultrasound (US) examination of the lateral surface of the 5th metatarsal.Results. 150 PsA patients (88 M; 62 F), 172 SpA (107 M; 65 F), and 95 healthy controls (58 M; 37 F) were evaluated. Based on clinical and US evaluation, bursitis was diagnosed in 17/150 (11.3%) PsA patients but in none of the SpA (P<0.0001) and healthy (P=0.0002) controls. In detecting bursitis, US was more sensitive than clinical examination, although the difference did not reach statistical significance (P=0.09).Conclusion. The bursa of the 5th metatarsophalangeal joint appears to be involved in PsA more frequently than by chance. If confirmed by other studies, this finding could be considered as a distinctive clinical sign of PsA, useful for differential diagnosis with the other SpA. In asymptomatic patients, US proved to be more sensitive in the detection of bursitis.

scholarly journals XXIII.—On the Disruptive Discharge of Electricity: An Experimental Thesis for the Degree of Doctor of Science, Department A

1878 ◽  
Vol 28 (2) ◽  
pp. 633-671 ◽  
Author(s):  
Alexander Macfarlane
Keyword(s):  
Copper Wire ◽  
Late Summer ◽  
The Other ◽  
Straight Line ◽  
Summer Session ◽  
The Difference ◽  
A Chain ◽  
The One ◽  
Metallic Parts ◽  
The University

The experiments to which I shall refer were carried out in the physical laboratory of the University during the late summer session. I was ably assisted in conducting the experiments by three students of the laboratory,—Messrs H. A. Salvesen, G. M. Connor, and D. E. Stewart. The method which was used of measuring the difference of potential required to produce a disruptive discharge of electricity under given conditions, is that described in a paper communicated to the Royal Society of Edinburgh in 1876 in the names of Mr J. A. Paton, M. A., and myself, and was suggested to me by Professor Tait as a means of attacking the experimental problems mentioned below.The above sketch which I took of the apparatus in situ may facilitate tha description of the method. The receiver of an air-pump, having a rod capable of being moved air-tight up and down through the neck, was attached to one of the conductors of a Holtz machine in such a manner that the conductor of the machine and the rod formed one conducting system. Projecting from the bottom of the receiver was a short metallic rod, forming one conductor with the metallic parts of the air-pump, and by means of a chain with the uninsulated conductor of the Holtz machine. Brass balls and discs of various sizes were made to order, capable of being screwed on to the ends of the rods. On the table, and at a distance of about six feet from the receiver, was a stand supporting two insulated brass balls, the one fixed, the other having one degree of freedom, viz., of moving in a straight line in the plane of the table. The fixed insulated ball A was made one conductor with the insulated conductor of the Holtz and the rod of the receiver, by means of a copper wire insulated with gutta percha, having one end stuck firmly into a hole in the collar of the receiver, and having the other fitted in between the glass stem and the hollow in the ball, by which it fitted on to the stem tightly. A thin wire similarly fitted in between the ball B and its insulating stem connected the ball with the insulated half ring of a divided ring reflecting electrometer.

scholarly journals Determination of the toxic threshold of lolitrem B in cattle eating endophye-infected perennial ryegrass

2007 ◽  
Vol 13 ◽  
pp. 399-402
Author(s):  
L. Blythe ◽  
C. Estill ◽  
J. Males ◽  
A.M. Craig
Keyword(s):  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
Clinical Signs ◽  
Positive Control ◽  
Control Group ◽  
Crossbred Cattle ◽  
Testing Laboratory ◽  
Neotyphodium Lolii ◽  
Lolitrem B

Perennial ryegrass (Lolium perenne) straw is used as a feed for livestock and horses. Some straw is infected with the endophyte, Neotyphodium lolii, which produces lolitrem tremorgens. Ingesting of the toxin produces clinical "ryegrass staggers." A 28 day feeding trail was conducted using 61 Black Angus, pure and crossbred cattle. The cattle were fed one of three levels of lolitrem B in chopped perennial ryegrass straw ranging from 0 ppb up to 3058 ppb. Four Japanese Wagyu crossbred cattle were fed chopped ryegrass straw containing 1400 ppb lolitrem B. The cattle were evaluated and scored twice a day for clinical signs of gait difficulties. Cattle consuming 1400 ppb lolitrem B did not show any overt clinical signs. After 14 days, 7 out of 15 cattle in the 1954 ppb lolitrem B group showed clinical signs of ataxia, stiffness, and tremors as seen in ryegrass staggers. All of the cattle in the positive control group of up to 3058 ppb lolitrem B with a 4 week mean of 2574 ppb developed clinical signs of staggers. None of the Wagyu cattle at 1400 ppb lolitrem B developed clinical signs. A dose response curve was developed based on the results of this study coupled with a prior study for use in the testing laboratory. Keywords: lolitrem B, perennial ryegrass, Lolium perenne, endophyte fungus, Neotyphodium lolii, cattle, threshold levels

The effect of deworming naturally infected heifers on liveweight gain, reproductive performance and fecal egg counts

1993 ◽  
Vol 73 (2) ◽  
pp. 287-293
Author(s):  
S. L. Boyles ◽  
L. J. Johnson ◽  
W. D. Slanger ◽  
B. J. Kreft ◽  
J. D. Kirsch
Keyword(s):  
Key Words ◽  
Reproductive Performance ◽  
Fecal Sample ◽  
Average Daily Gain ◽  
Treated Group ◽  
The Other ◽  
Control Group ◽  
Age At Puberty ◽  
Daily Gain

Twenty-six of 52 heifers with an average liveweight of 318 kg were dewormed by intraruminal injection of oxfendazole and placed in drylot on 31 January. The other 26 animals served as controls. All heifers were pastured starting on 14 June and the treatment heifers were dewormed in the same manner as at the start of the experiment (31 January) on 1 July and 31 July. The groups started out with similar total worm eggs per gram of fecal sample, but the dewormed heifers had fewer counts each month thereafter. The drylot average daily gain of the treated heifers was higher (P = 0.01) and less variable (P = 0.12) and by 29 May these animals were heavier by 11.7 kg (P = 0.10). The treated animals did not gain as fast on pasture (P = 0.20), but the gain continued to be less variable (P = 0.09). Age at puberty was not different. Number of open heifers in the treated group was 4 vs. 15% for the control group (P = 0.18). Deworming by intraruminal injection resulted in faster feedlot gains, more uniform gains during both drylot and pasture, reduced parasites and no abscess at injection sites. Key words: Anthelmintics, oxfendazole, cattle, heifers

Studies On The Dynamics Of Antithrombin III During Heparin Infusion By Radioimmunoassay

1981 ◽  
Author(s):  
S Urano ◽  
M Nakagawa ◽  
T Kitani ◽  
Y Maeda ◽  
M Watada ◽  
...  
Keyword(s):  
Plasma Level ◽  
Antithrombin Iii ◽  
Treated Group ◽  
Control Group ◽  
Heparin Infusion ◽  
Significant Difference ◽  
At Iii ◽  
Wet Weight ◽  
The Difference ◽  
Radioimmunoassay Method

A radioimmunoassay method for antithrombin III (ATIII) was developed in order to detect the AT III levels correctly in plasma and tissues and the effect of heparin infusion was investigated on rat using this method and 125I labeled ATIII. Rat AT III was purified from rat defibrinated plasma by heparin sepharose affinity chromatography and gel filtrations. This purified AT III was used for the preparation of specific AT III antiserum. Labeling of AT III with 125I was performed according to the method by Hunter and Greenwood. Plasma level of AT III were significantly decreased in the treated group with heparin for 6 hours, although significant difference was not observed in AT III contents in various organs. The behavior of i.v. injected AT III laveled with 125I in the normal control and treated groups proved the difference on the half life of AT III. Control group gave 52 hours and it was shortened in the treated group. The percent radioactivity per ml plasma after 6 hours of heparin infusion was 1.16±0.51, and 2.01±0.38 in the control group, and significant difference was observed (p < 0.05). On the contrary the percent dose radioactivity per g tissue wet weight was significantly increased in the liver, lungs, and large intestine on the heparin treated group. The decreased amount of the intravenously injected laveled AT III appears to be trapped and metabolized in the various organs mainly in the liver during heparin infusion. The decrease of plasma AT III levels on the patients treated with heparin may be explained from these experimental results.

A Discrimination Based on Punishment

1952 ◽  
Vol 4 (1) ◽  
pp. 27-45 ◽  
Author(s):  
James A. Dinsmoor
Keyword(s):  
The Other ◽  
Control Group ◽  
Experimental Session ◽  
Group 4 ◽  
White Rats ◽  
The Difference ◽  
Avoidance Reactions ◽  
Experimental Group ◽  
Rate Of Response

Twelve white rats learned to press a bar or lever when this act was intermittently followed by pellets of food. Once a stable rate of pressing had been established, the animals were subjected to electric shock as a punishment for each response during alternate five minute periods within the experimental session. A difference in rates during the safe and punished phases was manifested both by the experimental group (8 rats), who were provided with a light as a signal when it was safe to respond, and also, contrary to expectation, by the control group (4 rats), who continued in darkness throughout the session. The differential responding by the control group was greatly reduced, however, when the duration of each phase was reduced from five minutes to one. The investigation was then continued to determine the role of some of the other factors in the situation. Increasing the severity of the shock increased the difference between the light and the dark rates. Withholding the pellets of food reduced the overall rates but did not affect the light-dark discrimination. Withholding the shock, on the other hand, permitted an increase in rate and caused the animals to lose their discrimination; when food had previously been withheld, however, so that the rate of response was relatively low, this deterioration in the discrimination was not as rapid as before. Restoration of the discrimination training under a lower hunger drive confirmed the finding that the formation of the discrimination was quite rapid and showed that the level of drive did not affect the proportionality between the rates in the light and in the darkness. These findings were interpreted by comparing the current procedure with those previously used in studies of avoidance reactions and conflict.

The Date of the Adonia

1940 ◽  
Vol 60 ◽  
pp. 95-95
Author(s):  
A. S. F. Gow
Keyword(s):  
Late Summer ◽  
The Other ◽  
Early Autumn

When discussing the date of the Adonia at Alexandria I stated, on the authority of Dr. F. Heichelheim, that an unpublished papyrus in the Rylands collection connected Adonis with the late-Ptolemaic month Gorpiaios. P. Petrie 3.142, which Glotz had shown to relate to the Adonia, gives the 7th of an unnamed month as the date, and, assuming the month to be supplied by the Rylands papyrus, I placed the festival on one of the first days of September. After further study of the Rylands papyrus Dr. Heichelheim now thinks that it contains no mention of Adonis, and this combination therefore falls to the ground. A date in the late summer or early autumn is in my opinion sufficiently established by the other evidence, and as my argument is therefore not much affected, I had intended to withhold this correction until publication of the Rylands papyrus enabled me to supply a precise reference to it. In the present circumstances, however, it seems desirable to place it on record now.

scholarly journals Immature Platelet Fraction: A Useful Marker for Identifying the Cause of Thrombocytopenia and Predicting Platelet Recovery

Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 128-128
Author(s):  
Young Kyung Lee ◽  
Kibum Jeon ◽  
Miyoung Kim ◽  
Eunyup Lee ◽  
Jiwon Lee ◽  
...  
Keyword(s):  
Platelet Count ◽  
Cancer Patients ◽  
The Other ◽  
Discriminatory Power ◽  
Control Group ◽  
Platelet Recovery ◽  
P Values ◽  
Platelet Counts ◽  
Immature Platelet Fraction ◽  
The Difference

Abstract Introduction: The assessment of thrombopoietic activity in bone marrow is necessary for patients with thrombocytopenia to achieve correct diagnoses and effective treatments. We evaluated the discriminatory power of the immature platelet fraction (IPF%) in differentiating hyperdestructive/consumptive thrombocytopenia from hypoproductive thrombocytopenia, and its potential use as a predictive marker for platelet recovery. Methods: Platelet indices including IPF% were measured in 105 healthy individuals (the control group), 31 patients with hyperdestructive/consumptive thrombocytopenia (14 with immune thrombocytopenic purpura [ITP] and 17 with liver cirrhosis), and 34 patients with hypoproductive thrombocytopenia (4 with aplastic anemia and 30 with cancer who were undergoing chemotherapy) by using a Sysmex XN-3000 hematology analyzer. Results: The platelet number in the hyperdestructive/consumptive thrombocytopenia group was significantly lower than that in the hypoproductive thrombocytopenia group (P <0.001). The difference of plateletcrit (PCT) between the 2 thrombocytopenia groups was not statistically significant (P= 0.363). The IPF% was significantly higher in the hyperdestructive/consumptive thrombocytopenia group (median: 6.2% [IQR 4.3-10.3%]) than in both the control group (1.8% [1.3-2.4%]) and the hypoproductive thrombocytopenia group (1.8% [0.9-2.3%]) (all P-values < 0.001). However, the difference between the hypoproductive thrombocytopenia and control groups was not statistically significant (P= 0.331). Compared to the control group, the hyperdestructive/consumptive thrombocytopenia group showed a 190.5% increase in the median IPF% value, while the hypoproductive thrombocytopenia group showed a 9.5% decrease. The IPF# was significantly different between the 3groups; it was highest in the control group (median: 4.3 ×109/L [IQR 3.5-5.8×109/L]), followed by the hyperdestructive/consumptive thrombocytopenia group (3.2×109/L [1.7-4.5×109/L]), and lowest in the hypoproductive thrombocytopenia group (1.3 ×109/L [0.7-2.0×109/L]) (all P-values <0.001). Compared to the control group, the hyperdestructive/consumptive thrombocytopenia group showed a 25.6% decrease and the hypoproductive thrombocytopenia group a 69.8% decrease. PDW, MPV, and P-LCR were higher in the hyperdestructive/consumptive thrombocytopenia group (14.5 ± 3.0 fL, 12.0 fL [IQR: 11.0-12.0 fL], and 38.9 ± 6.8%, respectively) than in the other 2 groups, but there was no statistical difference between the other 2 groups. The IPF% in the hyperdestructive/consumptive group increased significantly as the number of platelets decreased, especially in patients with severe thrombocytopenia with platelet counts under 40.0 ×109/L. The IPF% was 4.7% (3.1-4.7%), 6.2% (3.2-7.6%), and 11.4% (7.1-16.6%) in patients with platelet counts>90.0 ×109/L, 40.0-90.0 ×109/L and<40.0 ×109/L, respectively (P = 0.010). However, this phenomenon was not observed in the hypoproductive thrombocytopenia group. The area under the curve (AUC) was highest for IPF% (0.938), indicating that this parameter showed the best discriminatory ability between the 2 groups, followed by PDW (0.885), P-LCR (0.859), IPF# (0.827), and MPV (0.824) (all P-values <0.001). The best IPF% cut-off value indicative of the highest sensitivity and specificity was 2.3%. The AUC of PCT was under 0.5 (0.445, P = 0.477), showing no discriminatory power. The IPF% decreased 3-4 days in advance of platelet count elevation in patients with ITP, while the IPF# ratio (compared to baseline) increased 3 days in advance of the same. Furthermore, the IPF% and IPF# ratio increased 5.5 days and 8.5 days, respectively, before platelet counts increased up to 130.0 × 109/L in cancer patients receiving chemotherapy. Conclusion: IPF% showed the most % value difference between the 3 groups and the largest AUC, showing it has the best discriminatory power in distinguishing the cause of thrombocytopenia. Also, IPF% showed an inverse correlation with platelet count in the hyperdestructive/consumptive thrombocytopenia group, but not in the hypoproductive thrombocytopenia group. The IPF% and IPF# were useful markers to predict the elevation of platelet count in advance in ITP and in cancer patients receiving chemotherapy. Disclosures No relevant conflicts of interest to declare.

scholarly journals Effect of Yeast (Saccharomyces cerevisiae) Supported by Selenium and Zinc on Lipid Profile of Local Sheep Males

2019 ◽  
Vol 12 (1) ◽  
pp. 89-96
Author(s):  
Mohannad Aremmt ◽  
T Mohammed ◽  
saad Alrawi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Synergistic Effect ◽  
Lipid Profile ◽  
Animal Health ◽  
The Other ◽  
Control Group ◽  
Organic Form ◽  
Yeast Saccharomyces Cerevisiae ◽  
The Third ◽  
Group 3

This study was conducted to investigate the effect of (Saccharomyces cerevisiae) supported with zinc and selenium on the lipid profile. of Twenty-one Iraqi males sheep were used aged (1-1.5) years and the weight mean approximately (38±4) kg. experiment animals used divided randomly into three treated groups each group have 7 males. All males were fed on the same ration. The first group was considered as a control group without treatment (T1), the second group (T2) were given yeast enriched with zinc (Saccharomyces cerevisiae -zinc) with dosage (0.2 g/kg/bw), the third group (3) was given yeast enriched with selenium (Saccharomyces cerevisiae-selenium) with dosage (0.03 g/kg/bw). The results showed significant (P≤0.05) differences control group compare with the other two-treated groups (T3 and T2) in triglyceride, LDL , VLDL and HDL . The results indicate to significant differences (P≤0.05) of (T2 and T3) compare with the control group by HDL . From the results concluded that there synergistic effect of Saccharomyces cerevisiae with zinc and selenium as organic form (Saccharomyces cerevisiae-zinc/selenium) could be attributed to improved animal health.

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