Soil acidity and growth of a legume. III. Interaction of lime and phosphate on growth of Medicgo sativa L. in relation to aluminium toxicity and phosphate fixation

1965 ◽  
Vol 16 (5) ◽  
pp. 757 ◽  
Author(s):  
DN Munns

On some acid sandy loam soils, lucerne required large additions of phosphate to grow normally unless lime was also applied. Plant symptoms, and analyses of plants, soil solutions, and 0.01M calcium chloride extracts, supported the hypothesis that, in the unlimed soils, additions of phosphate overcame aluminium toxicity as well as phosphorus deficiency. Both lime and large additions of phosphate lowered the concentrations of aluminium in the soil solutions and in the plants. Lime did not significantly affect either the equilibrium concentrations or the rates of release of phosphate in the soils. Yet at rates of phosphate adequate in the presence of lime, plants without lime looked phosphate-deficient, grew poorly, and usually contained deficient concentrations of phosphorus. The principal effects were consistent with effects of aluminium and phosphate in solution culture at the same concentrations as those observed in the soils. Aluminium toxicity was associated with 10 to 100 µM concentrations of aluminium in the soil solution and 3 to 10 µg atoms/g dry weight in the plant tops. Phosphorus deficiency was associated with phosphate concentrations of the order of 1µM or less in the soil solutions and 100 µg atoms/g or less in the plant tops.


1965 ◽  
Vol 16 (5) ◽  
pp. 743 ◽  
Author(s):  
DN Munns

Lucerne (M. sativa L.) grew as well in solutions maintained at pH 4 as at pH 5, and its growth at pH 4 was little affected by calcium concentrations above 5 mM when aluminium was not added (< 0. l µM). Phosphate concentrations above l µM were adequate for growth if maintained, and if phosphate was kept below 5 0 µM at pH 4 or below 10 µM at pH 4.5, then aluminium concentrations of the order of 100 µM could be maintained without evident reaction between aluminium and phosphate in solution. Under these conditions, uncomplicated by precipitation or phosphate deficiency in the nutrient solution, aluminium toxicity depressed yields, root elongation, and calcium and phosphate concentrations in shoots and roots, and it made the shoots look phosphate-deficient ; but it could not be remedied by increasing phosphate supply even when this restored plant phosphate to high levels. Only where addition of phosphate caused aluminium to precipitate in the solution did it alleviate the toxicity. Increasing the calcium concentration from 1 mM to 5 mM slightly alleviated the effects of aluminium, except at highly toxic concentrations. T. subterraneum took up more aluminium than lucerne, but tolerated aluminium in the medium better. Adding ethylenediaminetetra-acetate to solutions containing 200 µM aluminium concentrations improved the growth of both species to the extent that concentrations of free aluminium were reduced by chelation.



2003 ◽  
Vol 43 (5) ◽  
pp. 497 ◽  
Author(s):  
G. Ma ◽  
P. Rengasamy ◽  
A. J. Rathjen

Phytotoxicity of aluminium in acid soils is well known. At pH ≥6.3, aluminate [Al(OH)4–] is the principal hydroxo-aluminium species in soil solutions; however, its phytotoxicity has not received much attention. Sodic subsoils in Australia are generally alkaline and have pH above 9. During our survey of 8 subsoils in South Australia, we found aluminate ions at concentrations greater than 0.8 mg/L (29.7 μmol/L of aluminium) in soil solutions when pH was greater than 9, with corresponding high uptake of aluminium by wheat plants. We studied the phytotoxicity of aluminium to wheat plants in solution culture by maintaining the pH of alkaline solutions at 9.2.Relative root lengths of wheat plants, compared with those in reverse-osmosis deionised water, were significantly reduced in alkaline solutions and CO2-free air indicated toxicity of hydroxy, carbonate and bicarbonate ions. Further reduction of root lengths due to aluminate toxicity was also evident. Relative root lengths of wheat plants, when comparing between +aluminium and –aluminium treatments, were reduced up to 50% in alkaline solutions containing as low as 1 mg/L of aluminium. Aluminium accumulated mainly in the roots, thereby reducing their growth. In bicarbonate solutions, aluminium toxicity under alkaline pH was highly significant (P<0.001). However, at the same level of added aluminium in carbonate solutions, relative root length was not reduced. This study concludes that when aluminium species are present at a concentration of about 1 mg/L in soil solutions with pH greater than 9, the growth of wheat plants could be significantly affected.



PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e2963 ◽  
Author(s):  
Kiflemariam Y. Belachew ◽  
Frederick L. Stoddard

BackgroundFaba bean is an important starch-based protein crop produced worldwide. Soil acidity and aluminium toxicity are major abiotic stresses affecting its production, so in regions where soil acidity is a problem, there is a gap between the potential and actual productivity of the crop. Hence, we set out to evaluate acidity and aluminium tolerance in a range of faba bean germplasm using solution culture and pot experiments.MethodsA set of 30 accessions was collected from regions where acidity and aluminium are or are not problems. The accessions were grown in solution culture and a subset of 10 was grown first in peat and later in perlite potting media. In solution culture, morphological parameters including taproot length, root regrowth and root tolerance index were measured, and in the pot experiments the key measurements were taproot length, plant biomass, chlorophyll concentration and stomatal conductance.ResultResponses to acidity and aluminium were apparently independent. Accessions Dosha and NC 58 were tolerant to both stress. Kassa and GLA 1103 were tolerant to acidity showing less than 3% reduction in taproot length. Aurora and Messay were tolerant to aluminium. Babylon was sensitive to both, with up to 40% reduction in taproot length from acidity and no detectable recovery from Al3+challenge.DiscussionThe apparent independence of the responses to acidity and aluminium is in agreement with the previous research findings, suggesting that crop accessions separately adapt to H+and Al3+toxicity as a result of the difference in the nature of soil parent materials where the accession originated. Differences in rankings between experiments were minor and attributable to heterogeneity of seed materials and the specific responses of accessions to the rooting media. Use of perlite as a potting medium offers an ideal combination of throughput, inertness of support medium, access to leaves for detection of their stress responses, and harvest of clean roots for evaluation of their growth.



2021 ◽  
Vol 50 (4) ◽  
pp. 1195-1201
Author(s):  
Rifat Samad ◽  
Parveen Rashid ◽  
JL Karmoker

Increasing concentrations of aluminium progressively declined primary root length and number of lateral roots in rice and chickpea seedlings grown in rhizobox. It also inhibited the root and shoot length, dry weight of root and shoot of rice and chickpea seedlings grown in solution culture. On the other hand, it enhanced shoot/root length ratio and dry weight ratio for both the genera. Bangladesh J. Bot. 50(4): 1195-1201, 2021 (December)



Weed Science ◽  
1979 ◽  
Vol 27 (3) ◽  
pp. 278-279 ◽  
Author(s):  
W. S. Hardcastle

Twenty-eight commercial soybean [Glycine max(L.) Merr.] cultivars of maturity classes V through VIII were evaluated for differences in response to metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5(4H)-one] 0.125 ppm w/w in hydroponic culture. Top dry weight (TDW) of treated ‘FFR 666’ soybeans equaled that of the cultivar check and five other cultivars were not significantly different (P = 5%). ‘Semmes' was most sensitive to the herbicide with TDW 40% of cultivar check. ‘Tracy’ and ‘Coker 156’ were not significantly different (P = 5%) from Semmes. The other cultivars tested were intermediate in response to metribuzin.



1988 ◽  
Vol 110 (1) ◽  
pp. 119-140 ◽  
Author(s):  
G. N. Thorne ◽  
P. J. Welbank ◽  
F. V. Widdowson ◽  
A. Penny ◽  
A. D. Todd ◽  
...  

SummaryWinter wheat grown following potatoes on a sandy loam at Woburn in 1978–9, 1980–1 and 1981–2 was compared with that on a clay loam at Rothamsted in 1978–9 and 1980–1, and on a silty clay (alluvium) at Woburn in 1981–2. The cultivar was Hustler in the harvest years 1979 and 1981 and Avalon in 1982. On each soil in each year multifactorial experiments tested effects of combinations of six factors, each at two levels.The best 4-plot mean grain yield ranged from 89 to 11·1 t/ha during the 3 years; it was smaller on the sandy soil than on the clay soil in 1979, but larger on sand than on the clay in 1981 and 1982. Until anthesis the number of shoots, dry weight and N content of the wheat giving these best yields were less on sand than on clay. Unlike grain weight, straw weight was always less on sand.Sowing in mid-September instead of mid-October increased grain yield on clay in each year (by 0·4·0·7 t/ha) and increased yield on sand only in 1981 (by 1·6 t/ha). Early sowing always increased dry weight, leaf area, number of shoots and N uptake until May. The benefits were always greater on clay than on sand immediately before N fertilizer was applied in the spring and usually lessened later on both soils.Aldicarb as an autumn pesticide increased grain yield of early-sown wheat on both soils in 1981 by lessening infection with barley yellow dwarf virus. Aldicarb increased yield on clay in 1982; it also decreased the number of plant parasitic nematodes.Wheat on sand was more responsive to nitrogen in division, timing and amount than was wheat on clay. In 1979 yield of wheat on sand was increased by dividing spring N between March, April and May, instead of giving it all in April, and in 1982 by giving winter N early in February. In 1981 division and timing on sand interacted with sowing date. Yield of early-sown wheat given N late, i.e. in March, April and May, exceeded that given N early, i.e. in February, March and May, by 1·4 t/ha; single dressings given all in March or all in April also yielded less than the late divided dressing. Yield of later-sown wheat given all the N in April was at least 1·2 t/ha less than with all N given in March or with divided N. In all years treatments that increased yield usually also increased N uptake. Grain yield on clay was never affected by division or timing of spring N or by application of winter N. This was despite the fact that all treatments that involved a delay in the application of N depressed growth and N uptake in spring on both sand and clay. The mean advantage in N uptake following early application of spring N eventually reversed on both soils, so that uptake at maturity was greater from late than from early application. Increasing the amount of N given in spring from the estimated requirement for 9 t/ha grain yield to that for 12 t/ha increased yield in 1982, especially on sand. The larger amount of N always increased the number of ears but often decreased the number of grains per ear and the size of individual grains.Irrigation increased grain yield only on the sandy soil, by 1·1 t/ha in 1979 and by 07 t/ha in 1981 and 1982. The component responsible was dry weight per grain in 1979 and 1982, when soil moisture deficits reaching maximum values of 136 and 110 mm respectively in the 2 years developed after anthesis; the component responsible was number of ears/m2 in 1982 when the maximum deficit of 76 mm occurred earlier, in late May.



2016 ◽  
Author(s):  
Abdulaha-Al Baquy ◽  
Jiu-Yu Li ◽  
Chen-Yang Xu ◽  
Khalid Mehmood ◽  
Ren-Kou Xu

Abstract. Soil acidity has become a serious constraint in dry land crop production systems of acidic Ultisols in tropical and subtropical regions of southern China, where winter wheat and canola are cultivated as important rotational crops. Regardless of other common existing concerns in acidic Ultisols of southern China, it needs to be investigated whether soil acidity has any effect on wheat and canola growth. There is little information on the determination of critical soil pH as well as aluminium (Al) concentration for wheat and canola crops. The objective of this study was to determine the critical soil pH and exchangeable aluminium concentration (AlKCl) for wheat and canola production. Two pot cultures with two Ultisols from Hunan and Anhui were conducted for wheat and canola crops in a controlled growth chamber, with a completely randomized design. A soil pH gradient ranging from 3.7 (Hunan) and 3.97 (Anhui) to 6.5, with three replications, was used as a treatment. Aluminium sulfate (Al2(SO4)3) and hydrated lime (Ca(OH)2) were used to obtain the target soil pH levels. Plant height, shoot dry weight, root dry weight, and chlorophyll content (SPAD value) of wheat and canola were adversely affected by soil acidity in both locations. The critical soil pH and AlKCl of the Ultisol from Hunan for wheat were 5.29 and 0.56 cmol kg−1, respectively. At Anhui, the threshold soil pH and AlKCl for wheat were 4.66 and 2.36 cmol kg−1, respectively. On the other hand, the critical soil pH for canola was 5.65 and 4.87 for the Ultisols from Hunan and Anhui, respectively. The critical soil exchangeable Al for canola cannot be determined from the experiment of this study. The results suggested that the critical soil pH and AlKCl varied between different locations for the same variety of crop, due to the different soil types and their other soil chemical properties. The critical soil pH for canola was higher than that for wheat for both Ultisols, thus canola was more sensitive to soil acidity. Therefore, we recommend that liming should be undertaken to increase soil pH if it falls below these critical soil pH levels for wheat and canola production.



2019 ◽  
Vol 5 (04) ◽  
pp. 243-246
Author(s):  
Debnirmalya Gangopadhyay ◽  
Ashmita Ghosh ◽  
Mrinal Ray

Nitric oxide (NO) is an important bioactive signaling molecule in plants which modulates a variety of physiological processes and responses to abiotic and biotic stresses. In this study, the effects of exogenous NO supplied as sodium nitroprusside (SNP) in wheat seedlings under ironinduced oxidative damage was investigated. An appropriate concentration of NO was determined by conducting a preliminary experiment. In solution culture, wheat seeds were grown in the control (100 μM Fe), and toxic Fe (400 μM Fe) levels and the toxic Fe supply was treated with various levels of (50, 100, 200 and 500 μM) sodium nitroprusside (SNP). The results indicated that 400 μM Fe significantly decreased percentage germination, tolerance index, root lengths as well as fresh and dry weight compared to control. Exogenous SNP attenuated the inhibition of wheat seed germination. The promoting effect was most pronounced at 100 μM SNP. The accumulated concentration of iron and active Fe was significantly decreased by SNP treated Fe toxic seedlings. Toxicity of Fe caused oxidative stress by elevating hydrogen peroxide (H2O2), malondialdehyde (MDA) and proline contents in roots of wheat seedlings. One hundred μM SNP counteracted Fe toxicity by reducing the H2O2, MDA and proline contents of toxic Fe exposed seedlings. Meanwhile, application of SNP markedly reduced the activities of superoxide dismutases (SOD), catalases (CAT), peroxidase (POD), ascorbate peroxidases (APX), non protein thiols (NPT) and of glutathione reductase (GR) and increased ascorbate (ASc) compared with Fe toxic treatment alone, thereby indicating the modulation of the antioxidative capacity in the root under Fe stress by NO. The results indicated that the exogenous application of SNP, improved the antioxidant enzymes activity of wheat seedlings against Fe induced oxidative stress.



2014 ◽  
Vol 8 ◽  
pp. 14-22 ◽  
Author(s):  
D. Anbu ◽  
S. Sivasankaramoorthy

A pot culture was carried out with Oryza sativa L. vari-Co-39, to investigate the effects of supplementary calcium chloride on plants grown at NaCl (50mM) concentration. Treatments were: (1) Control: nutrient solution alone (C); (2) nutrient solution plus 50mM sodium chloride (NaCl); (3) nutrient solution plus 10mM calcium chloride (CaCl2); (4) nutrient solution plus 15mM calcium chloride (CaCl2); (5) nutrient solution and 50 mM NaCl plus supplementary 10 mM CaCl2 (NaCl + CaCl2); and (6) 50 mM NaCl plus additional mixture of 15 mM CaCl2 in nutrient solution (NaCl + CaCl2). The plants grown under salt stress produced low dry weight and relative water content than those grown in standard nutrient solution and in CaCl2 alone. Supplemental calcium chloride added to nutrient solution containing salt significantly improved growth and relative water content. Membrane permeability increased with high NaCl application and these increases in root membrane permeability were decreased with supplementary Ca. The concentration of chloride (Cl) increases highly for all treatments. Sodium (Na) concentration in plant tissues increased in both shoots and roots at high NaCl treatment. Application of supplementary Ca lowered Na concentration. Concentrations of Ca. K and N were at deficient ranges in the plants grown at high NaCl levels and these deficiencies were corrected by supplementary Ca. The ameliorating effect of Ca on growth and physiological variables could reduce the negative effect of salinity of Oryza sativa L., plants.



1992 ◽  
Vol 22 (2) ◽  
pp. 167-174 ◽  
Author(s):  
N.W. Foster ◽  
M.J. Mitchell ◽  
I.K. Morrison ◽  
J.P. Shepard

Annual nutrient fluxes within two forests exposed to acidic deposition were compared for a 1-year period. Calcium (Ca2+) was the dominant cation in throughfall and soil solutions from tolerant hardwood dominated Spodosols (Podzols) at both Huntington Forest (HF), New York, and the Turkey Lakes watershed (TLW), Ontario. There was a net annual export of Ca2+ and Mg2+ from the TLW soil, whereas base cation inputs in precipitation equaled outputs at HF. In 1986, leaching losses of base cations were five times greater at TLW than at HF. A higher percentage of the base cation reserves was leached from the soil at TLW (5%) than at HF (1%). Relative to throughfall, aluminum concentrations increased in forest-floor and mineral-soil solutions, especially at HF. The TLW soil appears more sensitive to soil acidification. Deposited atmospheric acidity, however, was small in comparison with native soil acidity (total and exchangeable) and the reserves of base cations in each soil. Soil acidity and base saturation, therefore, are likely only to change slowly.



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