The utilization and excretion of sulphur in cattle fed on tropical roughages

1974 ◽  
Vol 25 (6) ◽  
pp. 1015 ◽  
Author(s):  
PM Kennedy

The effect of additions of sulphateh to the diet of cattle given tropical spear grass (Heteropogon contortus) and Townsville stylo (Stylosanthes humilis) was studied in three experiments. In one experiment there was a small increase in the digestibility of dry matter, but the intake of feed was not affected. The concentration of volatile fatty acids in ruminal liquor from cattle offered spear grass was increased by supplemental sulphate; there was a concurrent small decrease in the proportions of propionic and isovaleric acids. Excretion of faecal sulphur varied with the intakes of total sulphur, organic matter, and digestible organic matter, while urinary sulphur excretion varied with the sulphur and organic matter intakes. Excretion of organic sulphur and total sulphate in urine and faeces increased with sulphur intake. The ratio of nitrogen to sulphur in faeces declined when sulphate was given. The retention of nitrogen was correlated with sulphur retention. It was concluded that the intake and digestion of the hays were not limited by the intake of sulphur.

1980 ◽  
Vol 94 (1) ◽  
pp. 219-227 ◽  
Author(s):  
C. G. Wernli ◽  
R. J. Wilkins

SummaryGrass silage (21.0 % D.M.) was given ad libitum to sheep together with supplements of rolled barley, dried-grass pellets or dried-grass wafers at 12 and 25 g organic matter (OM)/kg0·75.Intake of silage was greater with supplement at 12 g/kg0·75 but total intake of OM and digestible organic matter (DOM) increased with increasing supplement. Intake of OM was higher with the pellet supplement than with other supplements. Intake of DOM, however, did not differ between the pellet and barley treatments, but was lower with wafers.Concentrations of ammonia and total volatile fatty acids and the molar proportions of butyric and higher volatile fatty acids were higher when silage was supplemented with barley than with dried grass. Mean rumen retention times of silage and of supplement were highest in the barley-supplemented treatments. Dried-grass wafers were retained longer than dried-grass pellets. Supplement treatments did not differ significantly for eating and ruminating times, rumen fluid volume and pH, digestion rate in the rumen and nitrogen retention.At the low supplement rate silage consumption may have been controlled by factors associated directly with the silage, whereas at the high rate intake was limited either by physical factors or by the potential energy demand of the animals.


1991 ◽  
Vol 66 (1) ◽  
pp. 83-93 ◽  
Author(s):  
J. B. Schutte ◽  
J. de Jong ◽  
R. Polziehn ◽  
M. W. A. Verstegen

Hemicellulose consists primarily of pentose sugars, joined together in a polysaccharide chain with d-xylose as the most abundant component. Ileal digestibility and urinary excretion of d-xylose and associated effects of this pentose sugar on ileal and faecal digestibility of dry matter (DM), organic matter (OM), gross energy (GE) and nitrogen were studied in pigs. Castrated pigs were prepared with a post-valvular T-caecum cannula to measure ileal digestibility. Faecal digestibility was measured in non-cannulated pigs. d-xylose was given at dietary inclusion levels of 100 and 200 g/kg, and the control sugar, d-glucose, at a rate of 200 g/kg diet. Ileal digestibility of d-xylose as well as that of d-glucose was found to be close to 100%. The presence of d-xylose in the diet decreased ileal digesta pH and increased ileal flow of volatile fatty acids, suggesting the occurrence of microbial degradation of d-xylose in the pig small intestine. In pigs fed on the 100 g d-xylose/kg diet, 44.5% of the d-xylose intake appeared in the urine. This percentage increased significantly to 52.6 when pigs were fed on the 200 g d-xylose/kg diet. Ileal and faecal digestibility of DM, OM, GE and N, as well as N retention, decreased significantly in pigs fed on the 200 g d-xylose/kg diet.


2018 ◽  
Vol 5 (2) ◽  
pp. 11
Author(s):  
Ali Bain ◽  
Komang G. Wiryawan ◽  
Dewi Apri Astuti ◽  
Chairrusyuhur Arman ◽  
Sri Suharti

ABSTRAKPenelitian ini bertujuan untuk mengevaluasi optimalisasi penggunaan level sabun kalsium yang berbeda dalam ransum secara in vitro terhadap karakteristik fermentasi, populasi mikroba dan kecernaan nutrien ransum, menggunakan sumber inokulum cairan rumen sapi Bali. Penelitian dilaksanakan menggunakan rancangan acak kelompok dengan 4 jenis perlakuan dalam 3 ulangan.  Ransum penelitian terdiri atas : R1, 40% rumput lapang (RL) + 60% konsentrat (K), R2 (40% RL + 60% K, mengandungn 2.5% SCa-kedelai), R3 (40%  RL + 60% K, mengandung 5% SCa-kedelai), R4 (40%  RL + 60% K, mengandung 7.5% SCa-kedelai). Variabel yang diukur terdiri atas karakteristik fermentasi in vitro (pH, N-NH3, produksi total volatile fatty acids), populasi mikroorganisme (total bakteri dan total protozoa), dan kecernaan nutrien (kecernaan bahan kering dan kecernaan bahan organik). Data dianalisis menggunakan analysis of varians  (ANOVA) dan perbedaan antara perlakuan diuji dengan Duncan’s Multiple Range Test.  Hasil penelitian menunjukkan, penambahan SCa-kedelai pada level yang berbeda dalam konsentrat tidak mempengaruhi pH, konsentrasi amonia, populasi mikroba, kecernaan bahan kering dan kecernaan bahan organik fermentasi tetapi berpengaruh sangat nyata (P<0.002) terhadap produksi total VFA. Produksi total VFA tertinggi diperoleh pada ransum perlakuan R4 (konsentrat yang mengandung 7.5% SCa-kedelai). Produksi total VFA tertinggi diperoleh pada perlakuan R4 dan produksi total VFA paling rendah terjadi pada perlakuan ransum R1 (kontrol).  Produksi total VFA  ransum R1 dan R2 dan ransum R2 dan R3 tidak berbeda nyata namun produksi total VFA ransum R3 lebih tinggi dibanding ransum kontrol. Penambahan SCa-kedelai dalam konsentrat menghasilkan produksi total VFA tetinggi namun mulai menekan populasi total bakteri. Mempertimbangkan hasil peubah fermentasi dan populasi mikroba serta biaya pembuatan produk sabun kalsium, SCa-kedelai pada level 5% merupakan level penggunaan SCa-kedelai yang terbaik dalam ransum.Kata kunci: sabun kalsium, fermentasi, in vitro, kecernaanABSTRACTThe study to evaluate the optimalization the different level of soybean oil calcium soap (CaS-soybean) in ration on in vitro fermentation using Bali cattle rumen fluid. The experiment was arranged in a complete randomized block design with 4 different ration treatments and 3 replicates. Ration treatments were R1: 40% native grass (NG) + 60% concentrate (C), R2 : 40% NG + 60% C, supplemented with 2.5% soybean oil calcium soap (CaS-soybean), and R3 (40% NG + 60% C, supplemented with 5.0% CaS-soybean and R4 (40% NG + 60% C, supplemented with 7.5% CaS-soybean). Variables measured were fermentation characteristics (pH, NH3-N, total volatile fatty acids), microbial population (total bacteria and total protozoa), and nutrient digestibility (dry matter and organic matter digestibility). Data were analyzed using analysis of variance (ANOVA) and the differences between treatments means were examined by Duncan Multiple Range Test. Results of the study  showed that the four different level of CaS-soybean in ration did not have any significant effect (P>0,05) on pH, NH3-N, total bacteria, total protozoa, dry matter and organic matter digestibility. The treatments significantly increased (P<0.05) the production of total volatile fatty acids.  The highest production of total VFA was obtained from ration R4 (concentrate containing 7.5% CaS-soybean) and the lowest was obtained from treatment R1 (control). There were no significant differences between R1 and  R2, and between R2 and R3 on the total VFA production. However, the total productions of VFA in ration R3 were higher than that of the control ration. The addition of CaS-soybean in the concentrate had increased the total production of VFA, but at the same time, it began pressing the total population of bacteria. Considering the results of the fermentation variables and microbial population as well as the cost of making calcium soap products, CaS-soybean at 5% level was selected as the best level of CaS-soybean in ration.Keywords : calcium soap, fermentation, in vitro, digestibility


1969 ◽  
Vol 20 (5) ◽  
pp. 925 ◽  
Author(s):  
JP Hogan ◽  
RH Weston ◽  
JR Lindsay

A study was made of the composition, intake, and digestion of dried Phalaris tuberosa forage that had been harvested at three stages of maturity. Advancing maturity was associated with: (i) decreased food intake; (ii) increased expenditure of time and energy in chewing activities; (iii) decline in the rate of flow of digesta from the abomasum though not from the rumen; (iv) decline in digestibility in the whole tract of organic matter, nitrogen, and the structural carbohydrates; (v) decline in the digestion of fibre in the rumen relative to that occurring in the whole tract; (vi) decline in the quantities of volatile fatty acids and amino acids made available to the animal. By contrast only small differences attributable to maturity were observed in: (i) the distribution of digestion of organic matter between stomach and intestines; (ii) the digestibility of nitrogen other than ammonia in the intestines; (iii) the proportions of digestible organic matter derived from volatile fatty acids and amino acids; (iv) the proportions of individual amino acids in the digesta passing to the intestine. It was calculated that microbial piotcin contributed 33, 38, and 47% of the protein passing from the stomach to the intestincs with the diets of advancing maturity. Most of the remaining protein was presumably of dietary origin. About 80% of the crude protein in the digesta was present in the form of amino acids, and the quantities of amino acids released in the intestines were calculated to be equivalent to 64–66 g/100 g crude protein intake. With advancing maturity of the diets the plasma levels of the essential ammo acids except lysine, histidine, and arginine declined; there was little effect of diet on the plasma levels 01 non-essential amino acids. However, relative to total essential amino acids, the ratios of valine and leucine decreased with advancing maturity of the diet while those of lysine, histidine, glutamate, glycine, alanine, and serine increased. * Part 111, Aust. J. Agric. Res., 1969, 20, 347.


1977 ◽  
Vol 38 (3) ◽  
pp. 335-340 ◽  
Author(s):  
U. B. Singh ◽  
D. N. Verma ◽  
A. Varma ◽  
S. K. Ranjhan

1. The production rates of bacteria in the rumen of buffalo (Bos bubalis) calves were estimated using an isotope-dilution technique. A series of fifteen experiments was done with animals given green maize and nine experiments with animals given cowpea (Vigna unguiculata).2. The turnover time ranged from 205 to 567 min in the group given green maize and from 330 to 648 min in animals offered cowpea. The production rates of bacteria were (mean±SE; g/d) 145.77±7.240 and 237.09±11.847 in animals given green maize and cowpea respectively.3. There was a significant correlation between bacterial production rates and dry matter intake, digestible organic matter and total volatile fatty acids formed in the rumen.4. Regression equations obtained for the two foodstuffs were different suggesting that the bacterial growth rate may vary depending upon the quantity and quality of foodstuff digested and possibly the ratio nitrogen: energy of the foodstuff.


1972 ◽  
Vol 12 (57) ◽  
pp. 365 ◽  
Author(s):  
MJ Playne ◽  
KP Haydock

The voluntary dry matter intake, digestibility and chemical composition of Townsville stylo and of black spear grass cut at different times of the year from a site near Townsville, Queensland, have been examined using penned sheep. Nitrogen, sulphur, and phosphorus intakes and retention in the sheep were measured. The concentrations of N, P, S, Na, and Ca in most cuts of spear grass and P and Na in Townsville stylo were less than the published requirements of animals. With spear grass, digestible dry matter intake declined rapidly from January to April and more slowly from April to October and by mid-March fell below the estimated energy requirements for maintenance of sheep. There was little change in digestible dry matter intake of Townsville stylo between February and May and the level was well above maintenance requirements. Dry matter intake and dry matter digestibility were linearly related but only about 50 per cent of variations in intake were caused by variations in digestibility. Nitrogen balance was linearly related to nitrogen intake, and sulphur balance was linearly related to sulphur intake. Phosphorus intakes were usually less than 1 g P day-l and animals were usually in negative P retention.


1981 ◽  
Vol 32 (2) ◽  
pp. 231-233 ◽  
Author(s):  
B. Laksesvela

ABSTRACTWhole, moist barley treated with ammonia (A), whole, untreated barley (WU) and ground barley (G) were fed with hay and a little high protein, mixed concentrate. Digestibilities of dry matter, organic matter and crude fibre, assessed with young adult female sheep, were highest in diet A and lowest in diet G. The digestibilities of dry matter and crude fibre were also higher in diet WU than in diet G. Digestibility coefficients for dry matter and organic matter in barley, calculated by difference, appeared higher in A and in WU than in G. Young female sheep fed on the above diets from the age of 7 to 11 months ate more hay and gained more weight on A than on G; again diet WU was intermediate. Differences also occurred between the three groups as regards volatile fatty acids in the rumen fluid. Sheep on A or WU had a higher pH in the rumen fluid than those on G. Finally, sheep on A gave birth to more offspring than those on WU or G. All these differences were significant.


2021 ◽  
Vol 21 (2) ◽  
pp. 192-199
Author(s):  
Limbang Kustiawan Nuswantara ◽  
Eko Pangestu ◽  
Sunarso Sunarso ◽  
Marry Christiyanto

ABSTRACT. Penelitian bertujuan mengetahui kualitas complete feed dengan level pelepah sawit fermentasi berdasarkan kecernaan bahan kering, bahan organik, produksi N-NH3, produksi volatile fatty acids (VFA) dan produksi biomassa protein mikrobia serta protein total secara in vitro. Materi yang digunakan adalah complete feed tersusun atas konsentrat dan pelepah sawit fermentasi dengan berbagai level yaitu 0, 10, 20 dan 30%. Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) dengan 4 perlakuan complete feed dengan level pelepah sawit fermentasi yang berbeda. Data diolah menggunakan sidik ragam yang dilanjutkan dengan uji beda wilayah berganda Duncan. Hasil penelitian menunjukkan bahwa complete feed dengan level pelepah sawit fermentasi yang berbeda berpengaruh nyata (p0,05) terhadap kecernaan bahan kering dan bahan organik, produksi N-NH3, produksi VFA, dan produksi protein total, sedangkan pada biomassa protein mikrobia tidak terdapat perbedaan yang nyata (p0,05). Rata-rata nilai kecernaan bahan kering pada perlakuan T0, T1, T2 dan T3 adalah 69,59; 71,9; 69,05; dan 62,58%. Rata-rata nilai kecernaan bahan organik pada perlakuan T0, T1, T2 dan T3 adalah 63,59; 63,15; 65,50; 52,66 %. Rata-rata produksi VFA pada perlakuan T0, T1, T2 dan T3 sebesar 105,8; 142,7; 136,4; dan 135,7 mM. Rata-rata produksi NH3, biomassa protein mikrobia dan produksi protein total pada perlakuan T0, T1, T2 dan T3 berturut-turut adalah 6,48mM, 15,04mg/ml;, 34,10mg/g; 7,36mM, 15,75mg/ml, 23,72mg/g; 8,18mM, 12,59mg/ml, 33,72mg/g); dan 6,60mM, 15,31mg/ml, 40,80mg/g. Berdasarkan hasil penelitian disimpulkan bahwa penggunaan pelepah sawit fermentasi dengan level 20% dalam complete feed menghasilkan produksi VFA, kecernaan bahan kering dan bahan organik yang cukup baik sehingga dapat menjadi pakan alternatif sumber serat pengganti rumput. (Digestibility, fermentability and in-vitro production of microbial protein on complete feed based on fermented palm frond) ABSTRAK. This study aimed to determine the quality of a complete feed containing fermented palm fronds based on the digestibility of dry matter, organic matter, N-NH3, VFA, microbial protein biomass, and total protein in vitro. The material used was complete feed composed of concentrates and fermented palm fronds at various levels, i.e., 0, 10, 20, and 30%. The experiment was conducted as a completely randomized design (CRD) with four complete feed treatments containing different levels of fermented palm fronds. The data were processed using analysis of variance, followed by Duncan’s multiple range test. The results demonstrated that the complete feed with different levels of fermented palm fronds had a significant effect (p0.05) on the digestibility of dry matter and organic matter, N-NH3 production, essential fatty acids production, and total protein production, whereas there was no significant difference (p 0.05) on microbial protein biomass. The average dry matter and organic matter digestibility values of T0, T1, T2, and T3 treatments were 69.59; 63.59, 71.9; 63.15, 69.05; 65.50, and 62.58%; 52.66% respectively. The average production of volatile fatty acids of T0, T1, T2, and T3 treatments were 105.8; 142.7; 136.4; and 135.7 mM. respectively, while the average N-NH3 production, microbial protein biomass, and total protein production of the T0, T1, T2, and T3 treatments were 6.48, 7.36, 8.18, 6.60 mM; 15.04, 75, 12.59, 15.31 mg/ml; and 34.10, 23.72, 33.72, 40.80 mg/g. In conclusion, the use of fermented palm fronds at a 20% level in complete feed gave the best result in the production of volatile fatty acids, improved digestibility of dry matter, and organic matter, so it can be used as an alternative feed to replace grass fiber.


1972 ◽  
Vol 12 (57) ◽  
pp. 373 ◽  
Author(s):  
MJ Playne

Penned sheep were used to measure the voluntary dry matter intake, digestibility and chemical composition of Townsville stylo and of black spear grass fertilized at three different annual rates of superphosphate -0, 125 and 750 kg ha-1. Dry matter intake of spear grass fertilized at 125 kg ha-1 was greater (P<0.05) than that of spear grass fertilized at 750 kg ha-1, which in turn was greater than that of grass not fertilized (P<0.05). Dry matter digestibilities were generally similar, although the digestibility of the grass fertilized at 125 kg ha-l tended to be greatest. In Townsville stylo, no significant differences in intake or digestibility occurred with different fertilizer treatments.


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