Temperature effects on lignin, hemicellulose and cellulose in tropical and temperate grasses

1979 ◽  
Vol 30 (4) ◽  
pp. 621 ◽  
Author(s):  
CW Ford ◽  
IM Morrison ◽  
JR Wilson
Keyword(s):  
Cell Wall ◽  
Growth Temperature ◽  
Lignin Content ◽  
Significant Negative Correlation ◽  
In Vitro Digestibility ◽  
Panicum Maximum ◽  
Cellulose Content ◽  
Stage Of Development ◽  
Increasing Temperature

Thirteen tropical and 11 temperate grasses were grown in controlled environment under day/night temperatures of 21/13, 27/19 and 32/24°C. Neutral detergent fibre (NDF) was prepared from the fifth leaf on the main stem of each plant, 2 days after it had reached maximum length. Panicum maximum var. trichoglume (tropical) and Lolium perenne cv. S24 (temperate) were also harvested at 4,8, and 12 days after this stage of development. For the tropical grasses NDF values, corrected for starch and protein, decreased with increasing growth temperature, whereas in the temperate species they increased. In the tropical group the decrease in NDF was due to a lower cellulose content, whereas hemicellulose and lignin levels generally tended to increase slightly with increasing temperature. In the temperate grasses, lignin, hemicellulose and cellulose levels all showed a consistent increase with growth temperature. At each growth temperature the lignin content of the species in the tropical group had a significant negative correlation with in vitro digestibility, and lignin appeared to be more closely associated with hemicellulose than with cellulose. In contrast, at each temperature, variation in digestibility between species of the temperate group was not correlated with lignin. Levels of cell wall components in the later-harvested material from Lolium varied in a similar manner to that of the younger growth stage, whereas older Panicum cell wall constituents showed more variability.

Influence of growth temperature on anatomy and in vitro digestibility of maize tissues

1990 ◽  
Vol 114 (2) ◽  
pp. 207-212 ◽  
Author(s):  
J. W. Cone ◽  
F. M. Engels
Keyword(s):  
Cell Wall ◽  
Temperature Regime ◽  
Lignin Content ◽  
Chemical Properties ◽  
Wall Layer ◽  
Cell Diameter ◽  
In Vitro Digestibility ◽  
Cell Wall Digestibility ◽  
Temperature Regimes

SUMMARYTissues of maize grown under different temperature regimes showed remarkable differences in anatomical and chemical properties and in vitro digestibility. A high temperature regime (12 h at 30 °C and 12 h at 24 °C) resulted in decreased cell wall thickness, cell diameter and cell wall yield, doubled lignin content and decreased in vitro digestibility, compared with plants grown under a low temperature regime (12 h at 18 °C and 12 h at 12 °C). A reduction in intensity of staining for lignin was observed in plants grown at 30/24 °C. Cell wall digestibility was thought to be limited by an indigestible cell wall layer between the secondary walls of adjacent cells. The use of lignin staining was of limited value for predicting cell wall digestibility. High temperatures probably affect physiological processes leading to lignin formation and deposition.

EFFECT OF HARVEST DATES ON YIELD AND COMPOSITION OF FORAGE KALE

1989 ◽  
Vol 69 (1) ◽  
pp. 143-149 ◽  
Author(s):  
H. T. KUNELIUS ◽  
L. J. HALLIDAY ◽  
J. B. SANDERSON ◽  
U. C. GUPTA
Keyword(s):  
Brassica Oleracea ◽  
Crude Protein ◽  
Dry Matter ◽  
Lignin Content ◽  
In Vitro Digestibility ◽  
Cellulose Content ◽  
Cell Content ◽  
Harvest Dates ◽  
Brassica Oleracea L

Forage kale (Brassica oleracea L. ’Maris Kestrel’) was sown on 27 May and harvested at 18- to 22-d intervals between 16 Sept. and 6 Dec. in 1985 and 1986. Dry matter accumulated until early November and then declined at about 19 kg ha−1 d−1 until the final harvest. In vitro digestibility of dry matter (IVDDM) ranged from 916 to 952 g kg−1 among harvest dates. Crude protein was similar for most harvest dates. Hemi-cellulose content decreased in 1985 but not in 1986 while cellulose content decreased in 1986 but not in 1985. Lignin content increased from 25 to 43 g kg−1 between mid-September and early December. The cell content peaked at 814 g kg−1 in mid-November. There was an increase in S-methylcysteine sulphoxide (SMCO) concentration of leaves from 1.4 g kg−1 to 6.2 g kg−1 between the first and last harvest dates. Potassium concentrations increased in 1985 and decreased in 1986 from September to December while concentrations of P and S were not influenced by harvest dates. With later harvests, both Ca and Mg concentrations decreased while B decreased in 1985 but not in 1986. Zinc concentrations peaked in October in 1986 while in 1985 they were similar for the five harvest dates. Iron concentrations fluctuated in the two years while Cu and Mn concentrations were not influenced by harvest dates. Forage kale provided highly digestible dry matter in late season. The mineral composition of kale was adequate with the exception of Cu, Mn and Zn which would not satisfy the dietary requirements of ruminants. The SMCO concentrations in kale leaves increased steadily from mid-September to early December but were lower than those concentrations considered deleterious to animal health.Key words: Brassica oleracea L., crude protein, hemicellulose, cellulose, SMCO, macronutrients

Temperature influences on the in vitro digestibility and soluble carbohydrate accumulation of tropical and temperate grasses

1973 ◽  
Vol 24 (2) ◽  
pp. 187 ◽  
Author(s):  
JR Wilson ◽  
CW Ford
Keyword(s):  
Soluble Carbohydrates ◽  
Soluble Carbohydrate ◽  
In Vitro Digestibility ◽  
Controlled Environment ◽  
Growth Stages ◽  
Panicum Maximum ◽  
Carbohydrate Accumulation ◽  
Tropical Grasses ◽  
Stage Of Development

Thirteen tropical or subtropical and 11 temperate grasses were grown in controlled environment under daylnight temperatures of 21/13, 27/19, and 32/24�C. Each plant was harvested 2 days after the fifth leaf on the main stem reached maximum length; other plants of Panicum maximum var. trichoglume and Lolium perenne cv. S.24 grown under the same conditions were also harvested 4, 8, 12, and 18 days after this stage of development. There was a consistent decline in the in vitro digestibility of most of the temperate grasses with increase in temperature from 21/13 to 32/24�C. This decline averaged about 5 digestibility units and was associated with a fall in the percentage of soluble carbohydrates. In contrast, temperature had little effect on the digestibility of most of the tropical grasses. However, in later harvests of Panicum the digestibility at 27/19 and 32/24� was consistently lower than at 21/13�. This was attributed to the more advanced growth stages at the higher temperatures. The temperate grasses accumulated much higher concentrations of soluble carbohydrate than the tropical grasses.

Cell-Wall Constituents and In Vitro Digestibility of Napier Grass (Pennisetum Purpureum)

1973 ◽  
Vol 37 (5) ◽  
pp. 1255-1261 ◽  
Author(s):  
William L. Johnson ◽  
Javier Guerrero ◽  
Danilo Pezo
Keyword(s):  
Cell Wall ◽  
Napier Grass ◽  
Pennisetum Purpureum ◽  
In Vitro Digestibility

In situ degradability of organic matter, crude protein and cell wall of various tree forages

1992 ◽  
Vol 55 (1) ◽  
pp. 29-34 ◽  
Author(s):  
P. Kamatali ◽  
E. Teller ◽  
M. Vanbelle ◽  
G. Collignon ◽  
M. Foulon
Keyword(s):  
Cell Wall ◽  
Organic Matter ◽  
Leucaena Leucocephala ◽  
Crude Protein ◽  
In Vitro Digestibility ◽  
Sesbania Sesban ◽  
Holstein Friesian ◽  
Ruminal Degradation

AbstractLeaves of Leucaena leucocephala, Sesbania sesban and Calliandra callothyrsus were harvested in Rwanda from young shoots at 8 weeks after the first cutting. They were dried, ground and incubated at the same time in polyester bags in three non-lactating Holstein-Friesian cows each fitted with a ruminal cannula. The bags were removed at 0, 2, 4, 8, 24, 48, 72 and 144 h after the start of incubations. The different parameters characterizing extent and rate of ruminal degradation of organic matter (OM), neutral-detergent fibre (NDF), and crude protein (CP) were calculated. In vitro digestibility of residual protein after 24 h and 48 h incubation was also determined. Sesbania sesban had lowest cell wall contents and gave highest ruminal degradability for OM, NDF and CP. Leucaena leucocephala was degraded to a lesser extent, but its undegraded protein had a somewhat higher in vitro digestibility. In contrast, protein of Calliandra callothyrsus was poorly degraded and digested. The proportion and composition of cell wall could not explain these differences in digestion characteristics and other measurements, such as tannins, were incriminated. Increased ruminal incubation time augmented the extent of ruminal degradation and reduced in vitro digestibility of undegraded protein but did not affect the undigestible protein fraction.

scholarly journals The in vivo impact of MsLAC1, a Miscanthus laccase isoform, on lignification and lignin composition contrasts with its in vitro substrate preference

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Feng He ◽  
Katja Machemer-Noonan ◽  
Philippe Golfier ◽  
Faride Unda ◽  
Johanna Dechert ◽  
...  
Keyword(s):  
Cell Wall ◽  
Transcription Factors ◽  
Lignin Content ◽  
Lignin Biosynthesis ◽  
Lignin Composition ◽  
Xylem Fibers ◽  
Breeding Target

Abstract Background Understanding lignin biosynthesis and composition is of central importance for sustainable bioenergy and biomaterials production. Species of the genus Miscanthus have emerged as promising bioenergy crop due to their rapid growth and modest nutrient requirements. However, lignin polymerization in Miscanthus is poorly understood. It was previously shown that plant laccases are phenol oxidases that have multiple functions in plant, one of which is the polymerization of monolignols. Herein, we link a newly discovered Miscanthus laccase, MsLAC1, to cell wall lignification. Characterization of recombinant MsLAC1 and Arabidopsis transgenic plants expressing MsLAC1 were carried out to understand the function of MsLAC1 both in vitro and in vivo. Results Using a comprehensive suite of molecular, biochemical and histochemical analyses, we show that MsLAC1 localizes to cell walls and identify Miscanthus transcription factors capable of regulating MsLAC1 expression. In addition, MsLAC1 complements the Arabidopsis lac4–2 lac17 mutant and recombinant MsLAC1 is able to oxidize monolignol in vitro. Transgenic Arabidopsis plants over-expressing MsLAC1 show higher G-lignin content, although recombinant MsLAC1 seemed to prefer sinapyl alcohol as substrate. Conclusions In summary, our results suggest that MsLAC1 is regulated by secondary cell wall MYB transcription factors and is involved in lignification of xylem fibers. This report identifies MsLAC1 as a promising breeding target in Miscanthus for biofuel and biomaterial applications.

Prediction of forage digestibility from some laboratory procedures. 2. Comparison of in vivo digestibility at two institutes.

1971 ◽  
Vol 19 (2) ◽  
pp. 106-113
Author(s):  
B. Deinum
Keyword(s):  
Cell Wall ◽  
Organic Matter ◽  
Sulfuric Acid ◽  
Lignin Content ◽  
Forage Digestibility ◽  
Significant Difference ◽  
Laboratory Procedures ◽  
Sulfuric Acid Lignin

For part 1 see Abst. 1564, Vol. 40. 2. The relation between digestibilities of organic matter in vivo and in vitro did not differ significantly between the institutes at Hoorn and Wageningen. No difference was found in digestibility of the cell wall constituents in relation to the lignin content, nor was there a significant difference in the relation between the percentage of digestible cell contents and the percentage of cell contents in forage from well managed pastures. Twelve forages of highly digestible perennial ryegrass deviated from these farm forages at Wageningen, showing smaller excretion of bacterial and endogenous residue. Comparison of the 2 lignin procedures showed that 72% sulfuric acid lignin gave consistent residual standard deviations of digestibility of cell wall constituents; permanganate lignin gave smaller errors in forages at Wageningen but greater errors in forages at Hoorn. (Abstract retrieved from CAB Abstracts by CABI’s permission)

GENETIC VARIATION FOR FORAGE QUALITY OF ALFALFA STEMS

1987 ◽  
Vol 67 (4) ◽  
pp. 1057-1067 ◽  
Author(s):  
D. R. BUXTON ◽  
G. C. MARTEN ◽  
J. S. HORNSTEIN
Keyword(s):  
Cell Wall ◽  
Genetic Variation ◽  
Medicago Sativa ◽  
Crude Protein ◽  
Nutritive Value ◽  
Growth Traits ◽  
Forage Quality ◽  
In Vitro Digestibility ◽  
Medicago Sativa L

When normally harvested for hay, the basal portion of alfalfa (Medicago sativa L.) stems is low in forage quality. This study was conducted to determine whether genetic variation for in vitro digestible dry matter (IVDDM) and crude protein (CP) concentration of stems of perennial Medicago spp. could be identified and whether morphological and chemical traits were associated with variation in these characteristics of stems. The lower six nodes and internodes of stems and total herbage from 64 Plant Introduction (PI) accessions and five cultivars of field-grown plants were harvested twice when most plants were at the early seed-pod stage of maturity. Several morphological and growth traits were measured, and both stems and total herbage were evaluated for IVDDM and CP concentration. Covariant analysis was used to adjust in IVDDM and CP concentration of stems for differences in plant maturity. Significant variation for IVDDM and CP concentration in stems existed among the cultivars and among the accessions. Some accessions had significantly higher stem IVDDM or CP concentration than control cultivars. The M. sativa accession, PI 440496, had the fourth highest stem IVDDM and third highest stem CP, with concentrations of 374 and 79 g kg−1, respectively. The number of ridges on stems (stem shape) was significantly correlated with stem IVDDM and CP concentration with average correlation coefficients of 0.30 and 0.35, respectively. Cell-wall concentration and lignin concentration per unit cell wall of stems were both negatively correlated with stem IVDDM and CP concentration. Selection for improved nutritive value of stems may have potential for improving the nutritive value of alfalfa herbage.Key words: Alfalfa, forage quality, crude protein, in vitro digestibility, Medicago sativa L.

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