Some factors that contribute to poor survival of rhizobia on preinoculated legume seed

2012 ◽  
Vol 63 (9) ◽  
pp. 858 ◽  
Author(s):  
E. J. Hartley ◽  
L. G. Gemell ◽  
R. Deaker
Keyword(s):  
Cell Survival ◽  
Cell Number ◽  
Powder Materials ◽  
Seed Coating ◽  
Poor Survival ◽  
Point Of Sale ◽  
Desiccation Rate ◽  
The Status ◽  
Rate Of Decline ◽  
On Farm

Preinoculation of seed is a convenient alternative method to inoculating seed on-farm. With preinoculation, a range of plant-growth and protection agents, polymer adhesives, colour pigments or dyes, and powder materials may be incorporated into an inoculant adhesive-slurry prior to seed coating. However, our recent point-of-sale surveys support findings of previous studies that survival of rhizobia on preinoculated seed is variable and can be poor. We focussed our research, both in the laboratory and at commercial facilities, on some of the factors that may contribute to poor survival of rhizobia on preinoculated seed. We found that rhizobial survival was affected by water quality; filtration improved cell survival but was not equal to distilled water. We also found that polymers affected cell survival differently for each rhizobial strain, and that slowing the desiccation rate reduced the cell rate of decline. Although fewer in cell number, older inoculant afforded more protection for survival of rhizobial cells. There is a need to test each ingredient and stage in the seed-coating process for compatibility to determine the best practices to promote rhizobial survival on seed. Failure to act on these factors prolongs the status quo of the findings from recent retail surveys.

Improvement of Restaurant Operation by Sharing Order and Customer Information

2012 ◽  
pp. 241-257 ◽  
Author(s):  
Takeshi Shimmura ◽  
Takeshi Takenaka ◽  
Motoyuki Akamatsu
Keyword(s):  
Information Sharing ◽  
Real Time ◽  
Large Scale ◽  
Order Information ◽  
Point Of Sale ◽  
Customer Complaints ◽  
Staff Members ◽  
Customer Information ◽  
Service Staff ◽  
The Status

In full-service restaurants, it is important to share customer information among staff members in real time in order to perform complicated operations. Conventional point of sale (POS) systems in restaurants was developed to improve the verification and transmission of order information passed from the dining hall to the kitchen. However, POS systems have remained insufficient to share customers’ order information among many staff members in different positions. This paper introduces an information sharing system for full-service restaurants using an advanced POS system with which staff members can share order information in real time. Using this system, kitchen staff members can grasp the total number of orders and the elapsed time for preparation of each order. Moreover, service staff members can grasp the status of each customer quickly. Using this system in a large-scale restaurant, preparation processes can be made more efficient and reduce customer complaints.

scholarly journals High expression of EGFR predicts poor survival in patients with resected T3 stage gastric adenocarcinoma and promotes cancer cell survival

2017 ◽  
Vol 13 (5) ◽  
pp. 3003-3013 ◽  
Author(s):  
Daiyong Wang ◽  
Bing Wang ◽  
Ruohan Wang ◽  
Zaizhong Zhang ◽  
Youdong Lin ◽  
...  
Keyword(s):  
Cell Survival ◽  
Gastric Adenocarcinoma ◽  
Cancer Cell ◽  
High Expression ◽  
Poor Survival ◽  
Cancer Cell Survival

A phase III trial to evaluate the efficacy of uproleselan (GMI-1271) with chemotherapy in patients with relapsed/refractory acute myeloid leukemia.

2019 ◽  
Vol 37 (15_suppl) ◽  
pp. TPS7066-TPS7066 ◽  
Author(s):  
Daniel J. DeAngelo ◽  
Harry Paul Erba ◽  
Brian Andrew Jonas ◽  
Michael O'Dwyer ◽  
Paula Marlton ◽  
...  
Keyword(s):  
Cell Survival ◽  
Residual Disease ◽  
Leukemic Cells ◽  
Phase Iii ◽  
Chemotherapy Response ◽  
Poor Survival ◽  
Phase 3 ◽  
Pivotal Phase ◽  
Double Blind

TPS7066 Background: Binding of E-selectin (E-sel) to sialyl Lex, the E-sel ligand (E-sel-L), on the leukemic cell surface activates cell survival pathways and promotes chemotherapy resistance in AML. Expression of E-sel-L is associated with increased relapse and poor survival. Uproleselan (GMI-1271), a novel E-sel antagonist, disrupts cell survival pathway activation, enhances chemotherapy response and protects from toxicity such as mucositis with improved survival in vivo. Preclinical data support combination with multiple agents to achieve improved chemosensitivity and toxicities. A phase I/II study of uproleselan added to chemotherapy in R/R AML showed promising remission rates (CR/CRi) and survival outcomes, and reduced rates of mucositis. High E-sel-L expression on leukemic blasts in the bone marrow, rather than connoting treatment resistance and poor survival, instead correlated with longer survival than expected with addition of uproleselan. Breakthrough Therapy Designation was granted by FDA for treatment of patients with R/R AML. A pivotal phase 3 study (NCT03616470) is underway to assess the efficacy and safety of uproleselan with standard salvage chemotherapy in R/R AML. Methods: This study is a global, randomized, double-blind, phase 3 trial in adults aged 18-75 years with R/R AML and fit for chemotherapy. Patients may have primary refractory AML (received 1 prior induction containing an anthracycline and cytarabine), or be in untreated first or second relapse; prior HSCT is allowed. Treatment is MEC (mitoxantrone, etoposide, cytarabine) or FAI (fludarabine, cytarabine, idarubicin) induction regimen with blinded study drug (Uproleselan:placebo at 1:1 ratio) administered 1 day prior (sentinel dose) and then BID through 2 days post chemotherapy. Consolidation (HIDAC/IDAC) with uproleselan/placebo (same assignment) up to 3 cycles is allowed. The primary endpoint is overall survival; key secondary endpoints include the incidence of severe oral mucositis during induction and CR/CRh rate. Measurable residual disease, E-sel-L expression on leukemic cells, event free survival, safety, and pharmacokinetics will also be evaluated. Clinical trial information: NCT03616470.

scholarly journals SIRT1 and c-Myc Promote Liver Tumor Cell Survival and Predict Poor Survival of Human Hepatocellular Carcinomas

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e45119 ◽  
Author(s):  
Kyu Yun Jang ◽  
Sang Jae Noh ◽  
Nadja Lehwald ◽  
Guo-Zhong Tao ◽  
David I. Bellovin ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Survival ◽  
Liver Tumor ◽  
Poor Survival ◽  
Hepatocellular Carcinomas ◽  
Tumor Cell Survival ◽  
Liver Tumor Cell

A THRESHOLD MODEL FOR CELL SURVIVAL

2009 ◽  
Vol 02 (02) ◽  
pp. 119-127
Author(s):  
A. RANGAN ◽  
V. ARUNACHALAM
Keyword(s):  
Cell Survival ◽  
Threshold Model ◽  
Expected Number ◽  
Arbitrary Interval ◽  
The Status ◽  
A Cell ◽  
Stochastic Events

This paper deals with a threshold model for gene damages in a cell subject to repair. The damages and repairs are considered to be stochastic events. The status of the cell is partitioned into a number of states representing the number of proliferative gene damages it has suffered. The probability of finding the cell with a certain number of gene damages at any time and the expected number of repairs in an arbitrary interval are obtained.

The status of cattle genetic resources in North Ethiopia: On-farm characterization of six major cattle breeds

2007 ◽  
Vol 40 ◽  
pp. 15-32 ◽  
Author(s):  
M. Zerabruk ◽  
O. Vangen ◽  
M. Haile
Keyword(s):  
Production Systems ◽  
Field Measurements ◽  
The Other ◽  
Current Status ◽  
Cattle Breeds ◽  
Mixed Crop ◽  
The Status ◽  
Study Population ◽  
Secondary Information ◽  
On Farm

SummaryOn-farm surveys and characterisation of six indigenous north Ethiopian cattle breeds (Afar, Arado, Begait, Fogera, Medenes and Raya) was carried out with the objective of determining the current status of these breeds. Information from a structured questionnaire distributed to 450 farmers, 37 group discussions, field measurements of heart girth and height on 25 males and 25 females from each breed and secondary information were used to carry out the study. Population size of the Arado breed increased by more than three fold between 1992 and 1999, while the Raya, Fogera and Begait population decreased by57, 27 and 67%; between 1981 and 1999 respectively. Three distinctive types were identified within the Afar and Begait breeds with different adaptation and threat levels in relation to changes to the bio-physical and social environment. Overall production and reproduction performance of the breeds in the pastoral production system (Afar and Begait) was higher than the Raya and Medenes, and Arado and Fogera breeds of the agro-pastoral and mixed crop/livestock production systems respectively. Extinction probability for most ofthe breeds was high, the highest (0.67) being for the Begait breed. On the other hand, except for the initiative taken to evaluate, improve and conserve the Fogera breed at the Metekel and Andasa cattle breeding ranches, there are no institutionalized attempts towards improving and/or conserving the other breeds.

scholarly journals Glucose Regulates Rat Beta Cell Number through Age-Dependent Effects on Beta Cell Survival and Proliferation

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e85174 ◽  
Author(s):  
Zerihun Assefa ◽  
Astrid Lavens ◽  
Christophe Steyaert ◽  
Geert Stangé ◽  
Geert A. Martens ◽  
...  
Keyword(s):  
Beta Cell ◽  
Cell Survival ◽  
Cell Number ◽  
Age Dependent ◽  
Cell Survival And Proliferation

Effects of Carbon Tetrachloride on Neuronal Differentiation in Rat Embryo Mid-Brain Micromass Cultures

1989 ◽  
Vol 16 (3) ◽  
pp. 287-292
Author(s):  
Cecilia Clemedson ◽  
Erik Walum ◽  
Oliver Flint
Keyword(s):  
Carbon Tetrachloride ◽  
Cell Survival ◽  
Neuronal Differentiation ◽  
Neuronal Development ◽  
Neutral Red ◽  
Cell Number ◽  
Rat Embryo ◽  
Focus Formation ◽  
Drastic Increase ◽  
Development And Differentiation

The effects of carbon tetrachloride on neuronal development and differentiation have been studied. Rat embryo mid-brain micromass cultures were exposed for five days to 50, 100, 250, 500 or 1000ppm CCl4 in closed chambers. Differentiation was indicated by the formation of neuronal foci in the cultures. Effects on cell survival were estimated using a neutral red staining method. Carbon tetrachloride at 100ppm caused a drastic increase in neuronal differentiation, accompanied by a slight increase in total cell number. At 250ppm the number of differentiated foci was still elevated, but the outgrowth of neurites was markedly reduced. Neuronal differentiation and cell survival were reduced by 50% at a concentration of 430ppm. Using criteria previously defined for the identification of potentially teratogenic substances in the micromass system, CCl4 was classified as a non-teratogen. Nevertheless, the results show that a substance can cause inhibition of differentiation (reduction in neunte formation) without altering focus formation.

scholarly journals Down-regulation of PINCH-1 Reduces Expression of EGFR, ERK1/2 and c-Myc, and Leads to Loss of Cell Viability in Colon Cancer Cells

2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Birgitta Holmlund ◽  
Annica Holmqvist
Keyword(s):  
Colon Cancer ◽  
Cell Survival ◽  
Cancer Cells ◽  
Caspase 3 ◽  
Cell Number ◽  
Colon Cancer Cells ◽  
Down Regulation ◽  
Fluorescent Substrate ◽  
Antibody Arrays ◽  
The Relationship

Particularly interesting new cysteine-histidine rich protein (PINCH) is related to poor outcome in colorectal cancers. Here, the relationship between PINCH-1 and cell survival in colon cancer cells was analyzed and the signaling pathways regulated by PINCH-1 by using PINCH-1 siRNA. KM12C cells were treated with PINCH-1 siRNA or control siRNA. Cell number was analyzed by crystal violet staining and caspase-3 activity was assessed using a fluorescent substrate. PINCH-1 extra- and intracellular pathways in KM12C cells were investigated, using phospho-kinase/phospho-receptor tyrosine kinase (RTK) antibody arrays. The expression of c-Myc was evaluated by Quantitative real-time PCR (qPCR) and Western blot analysis. Cell number was significantly decreased (P=0.003) and the caspase-3 activity increased (P=0.019) in PINCH-1 depleted KM12C cultures compared to siRNA cultures. In PINCH-1 silenced KM12C cells, the levels of EGFR and ERK1/2 were significantly decreased (P=0.008, P=0.003, respectively) compared to their controls, as were the c-Myc mRNA and protein expressions (P=0.0073, P=0.0002, respectively). Down-regulation of PINCH-1 reduced the cell survival and lowers the levels of EGFR, ERK1/2 and c-Myc in colon cancer cells. PINCH-1 is essential for cell survival, and may be a future target for anticancer therapy. 

Measuring dimensions: the regulation of size and shape

Development ◽  
2000 ◽  
Vol 127 (14) ◽  
pp. 2977-2987 ◽  
Author(s):  
S.J. Day ◽  
P.A. Lawrence
Keyword(s):  
Cell Growth ◽  
Cell Survival ◽  
Cell Size ◽  
Characteristic Size ◽  
Cell Number ◽  
Morphogen Gradient ◽  
Average Cell Size ◽  
Average Cell ◽  
Size And Shape ◽  
Drosophila Wing

Over many years evidence has accumulated that plants and animals can regulate growth with reference to overall size rather than cell number. Thus, organs and organisms grow until they reach their characteristic size and shape and then they stop - they can even compensate for experimental manipulations that change, over several fold, cell number or average cell size. If the cell size is altered, the organism responds with a change in cell number and vice versa. We look at the Drosophila wing in more detail: here, both extracellular and intracellular regulators have been identified that link cell growth, division and cell survival to final organ size. We discuss a hypothesis that the local steepness of a morphogen gradient is a measure of length in one axis, a measure that is used to determine whether there will be net growth or not.

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