LED lights increase an antioxidant capacity of Arabidopsis thaliana under wound-induced stresses

2020 ◽  
Vol 47 (9) ◽  
pp. 853 ◽  
Author(s):  
Zahra Mirzahosseini ◽  
Leila Shabani ◽  
Mohammad R. Sabzalian
Keyword(s):  
Arabidopsis Thaliana ◽  
Light Emitting Diode ◽  
Light Condition ◽  
Dry Weight ◽  
Phenylpropanoid Pathway ◽  
Lipoxygenase Activity ◽  
Anthocyanin Synthesis ◽  
Red Led ◽  
Wound Stress ◽  
Led Lights

A comparison among four light emitting diode (LED) lights including red LED (R), blue LED (B), red (70%) + blue (30%) LED (RB) and white LED (W) as well as fluorescent (F) light was made on antioxidative capacity of Arabidopsis thaliana (L.) Heynh. in response to wounding. Under wound-stress condition, LED-exposed plants, especially RB-irradiated plants, maintained significantly higher shoot dry weight and antioxidant enzymes activities compared with those irradiated with fluorescent lights. The highest amounts of both chlorophyll a and b were observed in the leaves treated with B light. Also, the concentration of H2O2 was higher under the condition of RB and B lights compared with the other light environments. The highest amount of malondialdehyde was measured in plants exposed to F and B lights. Similarly, wounded leaves under F and B light conditions showed the maximum lipoxygenase activity, whereas R-exposed leaves had the lowest lipoxygenase activity. In contrast, the highest level of phenolic compounds was found in R and RB exposed leaves in response to wounding. Among the five light treatments, RB and B lights were more effective in stimulating anthocyanin synthesis; however, RB-exposed plants were more efficient in the late-induction of the PAL gene (phenylalanine ammonia lyase catalyses the first step of the general phenylpropanoid pathway). Collectively, we reasoned that RB light condition gives a superior capacity to Arabidopsis thaliana to tolerate wound-stress. Also, we propose the probable signalling role of ROS in light-stimulated wound responses in Arabidopsis.

scholarly journals Produksi selada (Lactuca sativa l.) dengan penyinaran lampu led merah dan biru di malam hari pada teknologi hidroponik sistem terapung termodifikasi

2018 ◽  
Vol 2 (1) ◽  
pp. 79 ◽  
Author(s):  
Ardiansyah Azhary Suhandoko ◽  
Sumarsono Sumarsono ◽  
Endang Dwi Purbajanti
Keyword(s):  
Lactuca Sativa ◽  
Nutrient Concentration ◽  
Light Emitting Diode ◽  
Dry Weight ◽  
Fresh Weight ◽  
Light Emitting ◽  
Blue Led ◽  
Lactuca Sativa L ◽  
Floating System ◽  
Led Lights

This research has an aim to observe the production of lettuce (Lactuca sativa L.) on hydroponic technology of floating system with red and blue LED (Light Emitting Diode) at night. This research was conducted inside the Screenhouse in which located on the top of Griya Kads House building, Bulusan Village, Tembalang Sub-district from May to June 2017. The research was conducted by 3x3 factorial experiments in Nested Design with 3 replications. The first factor was 0, 1.5 and 3 hours irradiation of red and blue LED lights at night. The second factor was 3, 5 and 7 ml/liter of AB mix nutrient concentration which was nested in the first factor. The data collected were fresh weight, dry weight and root canopy ratio. The results of the study showed that the treatment of AB mix nutrient concentration had no significant effect on all parameters. The production of lettuce with level of 5 ml concentration of AB mix was quite good, yet 3 ml and 7 ml levels didn’t improve lettuce production. The duration of red and blue LED irradiation only resulted in an increase in the production of canopy fresh weight. This research can be concluded that 3 hours was sufficient to give the good results to improve the results of lettuce plants production. Keywords : Lettuce Production, AB Mix Nutrition, Red and Blue LED.

scholarly journals Potential Use of Colored LED Lights to Increase the Production of Bioactive Metabolites Hedyotis corymbosa (L.) Lam

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 225
Author(s):  
Anh Tuan Le ◽  
Ju-Kyung Yu ◽  
Gyung-Deok Han ◽  
Thuong Kiet Do ◽  
Yong-Suk Chung
Keyword(s):  
Plant Growth ◽  
Crop Production ◽  
Genetically Modified Organisms ◽  
Light Emitting Diode ◽  
Photosynthetic Characteristics ◽  
Bioactive Metabolites ◽  
Total Phenolic ◽  
Blue Led ◽  
Red Led ◽  
Led Lights

Hedyotis corymbosa (L.) Lam is a wild herb that is used in traditional Indian, Chinese, and African medicine. Light-emitting diode (LED) technology is paving the way to enhance crop production and inducing targeted photomorphogenic, biochemical, or physiological responses in plants. This study examines the efficiency of H. corymbosa (L.) Lam production under blue 450 nm and red 660 nm LED lights for overall plant growth, photosynthetic characteristics, and the contents of metabolite compounds. Our research showed that blue LED lights provided a positive effect on enhancing plant growth and overall biomass. In addition, blue LED lights are more effective in controlling the production of sucrose, starch, total phenolic compounds, and total flavonoid compared to red LED lights. However, blue and red LED lights played essential but different roles in photosynthetic characteristics. Our results showed the potential of colored LED light applications in improving farming methods and increasing metabolite production in herbs. LED lights are safer alternatives than genetically modified organisms or genome editing.

scholarly journals Stimulation of Light-Emitting Diode Treatment on Defence System and Changes in Mesocarp Metabolites of Avocados Cultivars (Hass and Fuerte) during Simulated Market Shelf Conditions

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1654
Author(s):  
Semakaleng Mpai ◽  
Dharini Sivakumar
Keyword(s):  
Antioxidant Activity ◽  
Light Exposure ◽  
Light Emitting Diode ◽  
Persea Americana ◽  
Light Emitting ◽  
Fungicide Treatment ◽  
Led Light ◽  
Red Led ◽  
Simulated Market ◽  
Led Lights

The ability of light-emitting diode (LED) light treatment to reduce the anthracnose decay via its eliciting effects and thus induce resistance in the avocado (Persea americana), was investigated in this study to replace the current postharvest fungicide treatment. In experiment 1, the effect of blue or red LED lights (6 h per day) on the incidence of anthracnose in artificially inoculated (Colletotrichum gloesposorioides) and naturally infected avocados (cv. Fuerte and Hass) at 12–14 °C (simulated market shelf) for 4, 8, 14, and 16 days was investigated. In experiment 2, the effect of blue or red LED lights on the induced defence mechanism, fruit metabolites, antioxidant activity, and percentage of fruit reaching ready-to-eat stage was determined. Exposure to red LED light significantly reduced the anthracnose decay incidence in naturally infected cv. Fuerte on day 12 and in cv. Hass on day 16 compared to the prochloraz fungicide treatment by upregulating the PAL genes and maintaining the epicatechin content. Blue LED light accelerated the ripening in both cultivars, probably due to reduced D-mannoheptulose content. Red LED light exposure for 6 h per day and 12 days storage showed potential to replace the prochloraz treatment with improved ascorbic acid content and antioxidant activity.

scholarly journals Improvement of Growth and Morphology of Vegetable Seedlings with Supplemental Far-Red Enriched LED Lights in a Plant Factory

Horticulturae ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 109
Author(s):  
Hyunseung Hwang ◽  
Sewoong An ◽  
Byungkwan Lee ◽  
Changhoo Chun
Keyword(s):  
Light Emitting Diode ◽  
Red Light ◽  
Control Plant ◽  
Red Pepper ◽  
Hypocotyl Length ◽  
Light Emitting ◽  
Photostationary State ◽  
Yield And Quality ◽  
Red Led ◽  
Led Lights

Although light-emitting diode (LED) lamps have been broadly applied in horticultural production to improve plant yield and quality, compared to natural light there is a disadvantage in the lack of far-red light in the LED spectrum. Far-red light has been studied widely to control plant growth and development. Therefore, this study aimed to find the effect of supplemental far-red-enriched LED lights to control the growth of tomato, red pepper, cucumber, gourd, watermelon and bottle gourd seedlings. The treatments were cool white LED:far-red LED at ratios of 5:0, 5:1, 5:2 and 5:3. The growth of tomato and red pepper seedlings, including hypocotyl length, was correlated to far-red light and light intensity. The phytochrome photostationary state (PSS) value of maximum hypocotyl length by supplemental far-red-enriched light ranged from 0.69 to 0.77 in tomato and red pepper seedlings. Although hypocotyl lengths of cucumber and watermelon were greatly affected by PSS, the PSS value for maximum hypocotyl length was lower than for tomato and red pepper. These results show that manipulating supplemental far-red enrichment can be used to control vegetable seedling growth with some variation among plant species.

scholarly journals Effects of Light-Emitting Diodes on the Accumulation of Glucosinolates and Phenolic Compounds in Sprouting Canola (Brassica napus L.)

Foods ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 76 ◽  
Author(s):  
Chang Park ◽  
Nam Kim ◽  
Jong Park ◽  
Sook Lee ◽  
Jong-Won Lee ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Phenolic Compounds ◽  
Light Emitting Diode ◽  
Red Light ◽  
Dry Weight ◽  
Total Phenolic ◽  
Brassica Napus L ◽  
Light Emitting ◽  
Led Light ◽  
Sprout Growth

In this study, we investigated optimal light conditions for enhancement of the growth and accumulation of glucosinolates and phenolics in the sprouts of canola (Brassica napus L.). We found that the shoot lengths and fresh weights of red light-irradiated sprouts were higher than those of sprouts exposed to white, blue, and blue + red light, whereas root length was not notably different among red, blue, white, and blue + red light treatments. The accumulations of total glucosinolates in plants irradiated with white, blue, and red lights were not significantly different (19.32 ± 0.13, 20.69 ± 0.05, and 20.65 ± 1.70 mg/g dry weight (wt.), respectively). However, sprouts exposed to blue + red light contained the lowest levels of total glucosinolates (17.08 ± 0.28 mg/g dry wt.). The accumulation of total phenolic compounds was the highest in plants irradiated with blue light (3.81 ± 0.08 mg/g dry wt.), 1.33 times higher than the lowest level in plants irradiated with red light (2.87 ± 0.05 mg/g dry wt.). These results demonstrate that red light-emitting diode (LED) light is suitable for sprout growth and that blue LED light is effective in increasing the accumulation of glucosinolates and phenolics in B. napus sprouts.

scholarly journals LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1351
Author(s):  
Chia-Chen Chen ◽  
Maw-Rong Lee ◽  
Chi-Rei Wu ◽  
Hsin-Ju Ke ◽  
Hui-Min Xie ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Light Emitting Diode ◽  
Basal Medium ◽  
Gelling Agent ◽  
Embryogenic Calli ◽  
Chinese Medicinal Herb ◽  
Light Emitting ◽  
Light Spectra ◽  
Led Lights ◽  
Fritillaria Cirrhosa

Investigations were carried out to study the effects of light-emitting diode (LED) lights on growth and development of isosteroidal alkaloids in embryogenic calli of Fritillaria cirrhosa D. Don, an important traditional Chinese medicine herb. Calli were cultured in glass bottles, each containing 100 mL of Murashige and Skoog’s basal medium supplemented with 2% sucrose and 0.4% gellan gum powder, a gelling agent. These bottles were incubated in a specially designed plant growth chamber equipped with eight different LED lights consisting of single or combinations of four different light spectra emitting blue (450 nm), green (525 nm), red (660 nm), and far-red (730 nm) light. After three months of incubation, morphological changes in embryogenic calli were recorded, and LC-MS/MS analysis of cultures was carried out for peimisine, sipeimine, peiminine, and peimine. The highest number of somatic embryos and the maximum fresh weight was recorded in calli incubated under red (9R), infrared (9IR), and a combination of red+blue+infrared (3R3B3IR), respectively, in decreasing order. The highest contents of peimisine, peiminine, and peimine were recorded under red (9R) and infrared (9IR) lights, respectively. Eight LED lights had significant effects on the morphogenesis of embryogenic calli of F. cirrhosa D. Don and contents of isosteroidal alkaloids.

scholarly journals Impedance Measures and a Mounting Technique for Drosophila: Larval Movements, Heart Rate, Imaging, and Electrophysiology

2020 ◽  
Vol 3 (1) ◽  
pp. 12
Author(s):  
Noah de Castro ◽  
Robin Lewis Cooper
Keyword(s):  
Neuromuscular Junctions ◽  
Light Emitting Diode ◽  
Bright Light ◽  
Infrared Camera ◽  
Synaptic Function ◽  
Light Emitting ◽  
The Media ◽  
Led Lights ◽  
Suitable Technique ◽  
Larval Movement

Monitoring movements of larval Drosophila with electrical detection allows one to record the behaviors without the use of lights and cameras. This is a suitable technique when studying the use of light-sensitive proteins in optogenetic studies. Electrical measures are feasible to use in determining when a larva starts to move or continues to move after a light induced activation of channelrhodopsin. We have developed a technique using an electrical measure of the media as an index of larval movement. As a proof of concept, recordings with an infrared camera of the larval movement were simultaneous made with electrical measures. The two techniques parallel each other in their ability to index larval movements. Bright light-emitting diode (LED) lights used in optogenetic experiments tend to saturate the detectors of the camera unless filters are used and different filters maybe necessary depending on the LED spectrum and sensitivity of the camera. Impedance measures are independent of the type of LED or brightness. We also assessed the use of a non-solvent based glue (3M Vetbond) to hold larvae in place while measuring synaptic function of neuromuscular junctions, cardiac function and influence of modulators, or activation of light-sensitive channels.

scholarly journals Biohardening of Arabidopsis thaliana Seeds and Seedlings with Fraser Photinia Associated Bacterium (PGB_invit) in In vitro Conditions

2019 ◽  
Vol 47 (3) ◽  
pp. 954-961
Author(s):  
Maria BATOOL ◽  
Özlem AKKAYA ◽  
Mine GÜL ŞEKER ◽  
Yelda ÖZDEN ÇİFTÇİ
Keyword(s):  
Arabidopsis Thaliana ◽  
Photosynthetic Pigments ◽  
Growth Parameters ◽  
Endophytic Bacterium ◽  
Dry Weight ◽  
Bacterial Populations ◽  
Positive Effects ◽  
Inactive Form ◽  
Potential Possibility

The aim of this study was to analyze possible positive effects of putatively endophytic PGPB (PGB_invit), which was isolated from long-term in vitro cultured fraser photinia microshoots, on seed and 7-day old seedling stages of Arabidopsis thaliana. Seeds and in vitro-germinated seedlings were inoculated with 107 CFU/mL and 108 CFU/mL active (A) and inactive (I) endophytic bacterial populations along with their mix compositions (A+I) and suspended in MPYE broth together with their controls (untreated ones). 14 days old seedlings were evaluated for various plant growth parameters [i.e., shoot and root fresh weight (FW), shoot length (SL), shoot and root dry weight (DW), root length (RL) and photosynthetic pigments including chlorophyll a, b and carotenoids of plantlets] as well as endophytic and rhizospheric trait of bacteria. Positive effects of inactive and active bacterium on FW, DW and photosynthetic pigments for 7-day inoculated seedlings were recorded whereas an increase in photosynthetic contents for seed stage inoculations was observed. Rhizospheric and endophytic colonization of the bacteria was confirmed by PCR with the presence of virD1 gene, which is previously recorded to be existed in the plasmid of bacterium after inoculation. Overall, these results demonstrated that this peculiar putative endophytic bacterium being beneficial in active and even more useful in inactive form for A. thaliana when optimum conditions and concentrations are used. Moreover, presence of virD1 gene suggested its potential possibility to be used in bioengineering along with various other beneficial PGPR features as biofertilizer.

scholarly journals IN VITRO DEVELOPMENT OF YELLOW LAPACHO (BIGNONIACEAE) USING HIGH-POWER LIGHT EMITTING DIODE

2018 ◽  
Vol 42 (5) ◽  
Author(s):  
Ezequiel Enrique Larraburu ◽  
Gonzalo Sanchez Correa ◽  
Berta Elizabet Llorente
Keyword(s):  
In Vitro Culture ◽  
High Power ◽  
Stomatal Density ◽  
Light Emitting Diode ◽  
Dry Weight ◽  
Light Sources ◽  
Multiplication Rate ◽  
Light Emitting ◽  
Shoot Dry Weight

ABSTRACT Handroanthus ochraceus (yellow lapacho) is a medicinal, ornamental and timber tree which can be propagated by in vitro culture. Conventional methods use fluorescent lighting (FL), whereas light emitting diode (LED) has been used for this purpose only recently. The aim of this work was to evaluate the effects of FL and high-power LED (HP-LED) on the in vitro multiplication and rooting of yellow lapacho at different irradiances (15 to 60 µmol m-2s-1). Epicotyls obtained from half-siblings was multiplicated in WPM (Woody Plant Medium) supplemented with 20 µM benzilaminopurine and 1 mM IBA (indolebutiric acid). For rooting, shoots were cultured for 3 days in ½WPM supplemented with 50 µM IBA and for 42 days in auxin-free ½WPM under HP-LED or FL lighting. Under HP-LED, the multiplication rate of shoots increased significantly (61%) from 20 to 40 µmol m-2s-1 respect to FL. Differences in abaxial stomatal density and size were observed between light sources at 20 µmol m-2s-1. High HP-LED irradiance produced the highest rooting percentage. In the rooting stage, the marginal means of treatments without factors interaction showed that HP-LED irradiances significantly increased shoot length by 20%, shoot fresh weight by 77% and shoot dry weight by 30% in comparison to the values under FL. The maximum values calculated from the regression curves were around 50 µmol m-2 s-1 for HP-LED for all parameters except root lenght whereas were around 20 µmol m-2 s-1 for FL for all parameters except fresh and dry weigth of shoot. Here we show that HP-LED lighting improve in vitro culture of H. ochraceus, reduced 81% energy consumption respect to FL and uses only a multispectral LED instead of different single color LEDs. Therefore, HP-LED could be useful for the micropropagation of tree species contributing to sustainable agriculture and ecological restoration of degraded areas.

Discomfort glare caused by white LEDs having different spectral power distributions

2017 ◽  
Vol 50 (6) ◽  
pp. 921-936 ◽  
Author(s):  
WJ Huang ◽  
Y Yang ◽  
M Ronnier Luo
Keyword(s):  
White Light ◽  
Spectral Power ◽  
Light Emitting Diode ◽  
Light Emitting ◽  
White Leds ◽  
Significant Difference ◽  
Discomfort Glare ◽  
Led Lights ◽  
Green Leds

This paper describes an experiment to investigate discomfort glare caused by white light-emitting diode (LED) lights having different spectral power distributions. It included two groups: a ‘Metamerism’ group and a ‘correlated colour temperatures (CCT)’ group. In the former group, it was found that white lights at 7000 K constructed from different blue LEDs and the same red and green LEDs gave about the same glare perception. In the latter group, there was a significant difference in glare perception between white lights having different CCTs. Finally, glare models, including unified glare rating (UGR) and the newly derived QUGRspd, and mUGRspd models, were tested using the data from the experiment. All of them gave quite accurate predictions of the data.

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