150 SERUM TESTOSTERONE CONCENTRATIONS IN BULLS SUPPLEMENTED WITH RUMEN-PROTECTED FAT, ANTIOXIDANTS, OR BOTH

2014 ◽  
Vol 26 (1) ◽  
pp. 188
Author(s):  
M. M. Guardieiro ◽  
F. L. M. Silva ◽  
P. L. J. Monteiro ◽  
A. B. Nascimento ◽  
R. S. Gentil ◽  
...  

Testosterone metabolism occurs primarily in the liver, and circulating concentrations can be influenced by the amount and type of diet provided. Furthermore, there are reports of the influence of circadian rhythms on secretion of testosterone in ruminants. Based on evidence that supplementation with rumen-protected fat may increase circulating concentrations of steroid hormones (Guardieiro et al. 2010 Pesq. Agrop. Bras. 45, 408–414), this study aimed to evaluate the effect of the addition of rumen-protected fat, antioxidants, or both to the diet on serum testosterone concentration in bulls, and to assess whether there is variation in hormone concentrations at different times of the day after feeding. Forty-eight Nelore bulls were confined and assigned to four treatment groups according to the addition of rumen-protected fat, antioxidants, or both to the standard diet (sugarcane bagasse, citrus pulp, corn gluten meal, urea, and mineral salt): F) rumen-protected polyunsaturated fatty acids [PUFA; rich in linoleic, Megalac E®, QGN-Arm & Hammer, Rio de Janeiro, Brazil; 1.5% on dry matter (DM); n = 12]; A) antioxidant (a source of vitamin C and selenium, EconomasE®, Alltech Biotechnology, Buenos Aires, Argentina; 3 g/head/d; n = 12); FA) Megalac E® and EconomasE® (n = 12), or C) nothing (Control group; n = 12). After 75 days of offering diets, jugular blood samples were collected just before IV injections that were performed three times a day (7:00, 13:00, and 19:00 h) with saline (Control group, n = 24) or gonadotropin-releasing hormone (GnRH; 50 μg Gonadorelin, Fertagyl, MSD Saúde Animal, São Paulo, Brazil; GnRH group, n = 48) and 2 h afterward. Bulls were fed 1 h after the first IV GnRH/saline and the diets remained in the bunk for 17 h. Serum testosterone concentrations were measured by radioimmunoassay. Data were analysed by repeated-measures of GLIMMIX of SAS (SAS Institute Inc., Cary, NC, USA). As expected, the GnRH injections increased concentrations of testosterone after 2 h compared to saline (5.4 ± 0.16 v. 3.2 ± 0.16 ng mL–1; P < 0.001). However, after the last injection of GnRH there were lower (P < 0.001) concentrations of testosterone (2.0 ± 0.57) compared to other times. This fact may be explained by the probable association between the greater clearance of testosterone after feeding and the consumption of stock of pituitary LH by GnRH administration. However, there was no effect of diets on circulating testosterone concentration. We may suggest that males can more efficiently control steroid hormone concentrations than females by feedback mechanisms. Financial support was provided by CNPq, FAPESP, Arm & Hammer, Alltech, and EMBRAPA of Brazil.

2018 ◽  
Vol 68 (2) ◽  
pp. 193 ◽  
Author(s):  
V. KANOULAS ◽  
G. A. PAPADOPOULOS ◽  
G. ARSENOS ◽  
E. D. TZIKA ◽  
P. FORTOMARIS

The present study investigated the effects of attapulgite supplementation in sow diets during gestation and lactation on sow performance. The study comprised two reproductive phases (cycles) in two commercial farrow to finish farms: Farm A (capacity: 550 sows) and Farm B (capacity: 220 sows). The treatment groups were: a) control group (CN): the sows were fed a common gestation or lactation diet; b) attapulgite group (AT): the sows were fed the CN diet supplemented with attapulgite at 0,7% level; c) attapulgite plus group (AT+): the sows were fed the CN diet supplemented with attapulgite (0.7%) and a mix of enzymes, live yeast and amino acids (0.1%), at a total of 0.8% level. Within each cycle the sows included per treatment were: 24 for Farm A; 12 for Farm B. Initially data were analyzed per cycle and per each farm. Data from sows that completed both cycles within each farm, were analyzed by repeated measures analysis. Regarding sow parameters, sow body weight loss during lactation tended to be greater in AT sows compared to CN sows during cycle 1 in Farm B and was greater in AT and AT+ than CN sows in Farm A that completed both cycles (P=0.063 and P=0.023, respectively). A greater litter size 24h postpartum was observed in favour of AT compared to CN group during cycle 1 in Farm A and in sows that completed both cycles in Farm A (P=0.001 and P= 0.011, respectively). Litter size at weaning was greater in sows from the AT group than CN during cycle 1 and 2 in Farm A, in cycle 1 in Farm B and in sows that completed both cycles in Farm A (P=0.004, P=0.037, P=0.037, and P=0.022, respectively). Piglet weight at weaning and average daily gain during lactation were greater in AT group than CN and AT+ in sows that completed both cycles in Farm A (P=0.049 and P=0.040 respectively). Notable similar effects, although not statistically significant, were also observed in Farm B. This field study suggests that attapulgite supplementation in sow diets can improve performance indexes. Further research should investigate the underlying mechanisms involved.


2014 ◽  
Vol 5 (2) ◽  
pp. 95
Author(s):  
Ghulam Nabi ◽  
Muhammad Amin ◽  
Jeena Urooj ◽  
Muhammad Kamil ◽  
Ayaz Ali Khan

The objective of this study was to determine the effects of wood, cigarette and marijuana smoke on the reproductive health of tandoor occupants. A total of 100 male individuals were selected (50 control and 50 tandoor occupants). A standard questionnaire was designed regarding their age, economic status, marital status, fuel type, exposure time (per day), use of mask, addiction and reproductive health. Morning blood samples of 5 mL of the size were taken from all participants. Serums were obtained and analyzed for total serum testosterone concentration. Bio-check (USA) kit was used according to the manufacturer protocol and procedures for testosterone analysis. In control group the mean ± SEM of total serum testosterone was 671.9 ± 20.02 ng/dl where as in tandoor occupants it was 542.7 ± 16.40 ng/dl. There was a significant reduction (P**** < 0.0001) in total serum testosterone concentration in tandoor occupants as compared to control group. Reproductive health problems like, low libido, erection problems, infertility, decreased frequency for shaving and absent morning and nocturnal erection were common in tandoor occupants as compared to control group. Wood, cigarette and marijuana smoke negatively affects testosterone concentration and lowers it significantly. This reduced testosterone concentration then produces ill effects like low libido, erection problems, infertility and absent morning and nocturnal erection. 


1979 ◽  
Vol 90 (2) ◽  
pp. 328-335 ◽  
Author(s):  
B. Baranowska ◽  
S. Zgliczyński

ABSTRACT In order to elucidate the mechanism of disturbances of gonadal hormones secretion in anorexia nervosa 14 female patients were investigated. A control group also consisted of 14 women of the same age. The serum LH, progesterone, oestrogens: oestrone + oestradiol (Oe1 + Oe2), oestriol (Oe3) and testosterone were determined by radioimmunological methods. In patients with anorexia nervosa the serum testosterone and Oe3 concentrations were dramatically elevated, whereas LH, progesterone and Oe1 + Oe2 were decreased as compared with the control group. Considerable weight gain induced by cyproheptadine treatment caused a normalization of the serum testosterone and Oe3 concentrations in all the patients. A negative correlation between the testosterone level and the deficit in body weight was observed. The mechanism causing the dramatically high serum testosterone concentration in the female patients with anorexia nervosa is discussed.


2019 ◽  
Vol 12 (2) ◽  

The current study was presented to conclude the influence of different ratio of pomegranate peel powder (PP) in feed of Karadi male lambs, they divided to 4 groups (0% as a control, 1% treatment 1, 2% treatment 2 and 4% as treatment 3), on scrotal circumference (SC), scrotum height (SH), body weight (BW) and blood serum testosterone concentration (T). In this study, sixteen (16) of Karadi male lambs used with 24.30 ± 0.40 kg weight and with 4-5 months old, all lambs have been randomly divided equally into four (4) groups and individually penned in a box for during 63 days. Results in our study showed that SC was significantly different and recorded higher 17.875±0.54 cm in group that fed on 1% PP, also SH recorded higher and significant different in group fed on 1% PP compared with other groups 10.000±0.32 cm. Average blood serum testosterone reduces in all groups compared to control group that fed on 0% of PP (11.418±0.15 n.mol/L). Final body weight in our study had significantly different (P < 0.05) between groups, 1% PP recorded higher weight (34.9 ± 0.58) kg. Each average daily gain, total body gain and initial body weight had not significant different in all groups. Correlation coefficient between SC, SH, blood serum testosterone concentration and final BW computed and ranged between (-0.397 to + 0.914).


Author(s):  
J.J. Rokade ◽  
S.K. Bhanja ◽  
A.S. Shinde ◽  
Sajjad, Darshana ◽  
B. Bhaisare ◽  
...  

The present study was undertaken to evaluate the efficacy of aspirin as anti-heat stressor. Broiler chicks were reared on a standard diet up to 14 days of age. Thereafter, the chicks were randomly distributed into three dietary treatment groups viz., T1 (Control group: Standard diet), T2 (Standard diet with aspirin@250 mg/kg) and T3 (Standard diet with aspirin@500 mg/kg). Each treatment was having five replicates of eight birds per replicate. Experiment was carried out during hot-dry (April-May, 30.0±0.70 to 37.0±1.40C, Rh, %: 58.05±1.32 to 70.11±0.82) summer. Feed intake and live weight gain was comparable among the treatments but FCR, protein and energy efficiency improved significantly (P less than 0.001) on aspirin supplementation. The relative yield of thymus and bursa at 4th and 6th week of age improved significantly (P less than 0.05). The percentage of protein, aspartate transaminase (AST) and alanine transaminase (ALT) increased significantly (P less than 0.001) due to aspirin supplementation at 4th as well as 6th week of age. While H:L ratio, serum corticosteron and serum cholesterol (only at 42nd day of age) decreased significantly (P less than 0.001) in aspirin supplemented groups at 28th as well as 42nd day of age. Supplementation of aspirin at 500 mg/kg reduced relative expression of HSP70 in jejunum tissues during 28th or 42nd day of age. It can be concluded that aspirin supplementation 500 mg/kg in diet of heat stressed broilers improved performance as well as welfare..


2017 ◽  
Vol 32 (3) ◽  
pp. 305-310 ◽  
Author(s):  
James M. Burgert ◽  
Arthur D. Johnson ◽  
Jose Garcia-Blanco ◽  
Lawrence V. Fulton ◽  
Michael J. Loughren

AbstractIntroductionThe American Heart Association (AHA; Dallas, Texas USA) and European Resuscitation Council (Niel, Belgium) cardiac arrest (CA) guidelines recommend the intraosseous (IO) route when intravenous (IV) access cannot be obtained. Vasopressin has been used as an alternative to epinephrine to treat ventricular fibrillation (VF).Hypothesis/ProblemLimited data exist on the pharmacokinetics and resuscitative effects of vasopressin administered by the humeral IO (HIO) route for treatment of VF. The purpose of this study was to evaluate the effects of HIO and IV vasopressin, on the occurrence, odds, and time of return of spontaneous circulation (ROSC) and pharmacokinetic measures in a swine model of VF.MethodsTwenty-seven Yorkshire-cross swine (60 to 80 kg) were assigned randomly to three groups: HIO (n=9), IV (n=9), and a control group (n=9). Ventricular fibrillation was induced and untreated for two minutes. Chest compressions began at two minutes post-arrest and vasopressin (40 U) administered at four minutes post-arrest. Serial blood specimens were collected for four minutes, then the swine were resuscitated until ROSC or 29 post-arrest minutes elapsed.ResultsFisher’s Exact test determined ROSC was significantly higher in the HIO 5/7 (71.5%) and IV 8/11 (72.7%) groups compared to the control 0/9 (0.0%; P=.001). Odds ratios of ROSC indicated no significant difference between the treatment groups (P=.68) but significant differences between the HIO and control, and the IV and control groups (P=.03 and .01, respectively). Analysis of Variance (ANOVA) indicated the mean time to ROSC for HIO and IV was 621.20 seconds (SD=204.21 seconds) and 554.50 seconds (SD=213.96 seconds), respectively, with no significant difference between the groups (U=11; P=.22). Multivariate Analysis of Variance (MANOVA) revealed the maximum plasma concentration (Cmax) and time to maximum concentration (Tmax) of vasopressin in the HIO and IV groups was 71753.9 pg/mL (SD=26744.58 pg/mL) and 61853.7 pg/mL (SD=22745.04 pg/mL); 111.42 seconds (SD=51.3 seconds) and 114.55 seconds (SD=55.02 seconds), respectively. Repeated measures ANOVA indicated no significant difference in plasma vasopressin concentrations between the treatment groups over four minutes (P=.48).ConclusionsThe HIO route delivered vasopressin effectively in a swine model of VF. Occurrence, time, and odds of ROSC, as well as pharmacokinetic measurements of HIO vasopressin, were comparable to IV.BurgertJM, JohnsonAD, Garcia-BlancoJ, FultonLV, LoughrenMJ. The resuscitative and pharmacokinetic effects of humeral intraosseous vasopressin in a swine model of ventricular fibrillation. Prehosp Disaster Med. 2017;32(3):305–310.


2021 ◽  
Vol 2 ◽  
Author(s):  
Ifeanyichukwu Chukwuemeka Egbuniwe ◽  
Chukwuka Nwocha Uchendu ◽  
Ikechukwu Reginald Obidike

Abstract The experiment investigated the effects of dietary ascorbic acid and betaine stress responses, serum testosterone levels, and some sexual traits in male Japanese quails during the dry season. A total of 240 male Japanese quails (14 days old) were used and randomly assigned to four groups, each group has three replicates (n = 20). Birds in treatment groups were fed ascorbic acid (AA); betaine (BET); and AA + BET in their diets, whereas the control birds were fed only basal diet. Environmental conditions were predominantly outside thermoneutral zone for Japanese quails. Dietary AA ± BET increased (p < .05) serum catalase, reduced glutathione and testosterone, but lowered (p < .05) cortisol levels when compared with control group. Supplemental AA, BET, or AA + BET enhanced (p < .05) cloacal gland size and sexual traits. In conclusion, dietary AA and BET improved stress responses, serum testosterone levels, and some sexual traits in male Japanese quails during the dry season.


2019 ◽  
Vol 21 (6) ◽  
pp. 497-505
Author(s):  
Lilik Herawati ◽  
Kristanti Wanito Wigati ◽  
Purwo Sri Rejeki ◽  
Widjiati Widjiati ◽  
Roedi Irawan

Background: Several caloric restriction studies revealed good for diabetes prevention. However, prevalence of it seems rising yearly. It needs alternative technique thus people can choose suitable way for them. Aim: To determine the effect of glucose diet intermittently on pancreatic duodenal homeobox-1 (PDX-1), apoptosis in pancreatic islets, and pancreatic islets area. Materials and methods: Balb/c mice were divided into five groups. Control group was given standard diet. The Continuous group was given standard diet and added with 7.4% calories continuously. The 1x, 2x, and 3x intermittent groups were given standard diet and added 7.4% calories for 1x, 2x, and 3x/week respectively. The 7.4% calorie addition was a glucose solution by oral galvage and ad libitum for 8 weeks. Results: There was a significantly difference on apoptosis density (p=0.043), but not in PDX-1. The islets Int2x and Int3x groups showed a significant decrease than control group (p=0.048). Insulin serum levels increased significantly in Continuous group compared to control group (p=0.04). In addition, the insulin serum level of 1x and 3x intermittent groups were significantly lower than Continuous group (p0.05). Pre-post blood glucose levels on treatment groups decreased significantly compared to control group (p=0.012). Conclusions: Continuous and 1-3x/week intermittent addition of 7.4% calories of glucose for 8 weeks indicate a compensation mechanism for maintaining homeostasis, such as increase insulin serum level and seem to initiate the changes of morphologic-biomolecular (mainly apoptosis density in islets). The better mode is 1x/week of additional calories. However it needs further exploration to find out other influenced factors for these mechanism discovery.


2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Idris Sulaiman Abubakar ◽  
Ayyuba Rabiu

Consumption of potash extract is traditionally used for suppression of male sexual desire and as a fertility control method even before the emergence of modern contraceptives. This research was aimed at determining potash extract’s role in suppression of male sexual desire. Four groups of virgin Wister rats were formed of which three groups were given different doses of potash extract and the fourth group as control. They were monitored for sexual desire with CCTV cameras and were sacrificed after 28 days. Serum testosterone and estrogen levels were assayed and sperm analysis was carried out. Data were analyzed using SPSS version 19 Statistical Software. A p<0.05 was considered as significant. Findings showed that higher doses of this potash extract (500 mg/kg and 1,000 mg/kg) stimulated more sexual desire, evidenced by increased duration of mounting. However, Serum testosterone and estrogen levels as proxy markers of sexual desire were found low in a dose dependent manner and with narrow margin of effects. The study showed no statistically significant difference between the control group and different treatment groups of potash extract on parameters of sperm analysis.


1995 ◽  
Vol 18 (4) ◽  
pp. 293-309 ◽  
Author(s):  
Virginia W. Berninger ◽  
Robert D. Abbott ◽  
Diane Whitaker ◽  
Leihua Sylvester ◽  
Susan B. Nolen

Twenty-four children with writing problems were given instruction in handwriting automaticity, spelling strategies, and the composing process (plan, write, review, revise) in 14 one-hour individual tutorials during the summer between third and fourth grade. Half the children (8 boys, 4 girls) received extra practice in composing, while half the children (8 boys, 4 girls) received special training in orthographic and phonological coding. Hierarchical linear modeling of growth curves was used to compare the treatment groups to a non-contact control group (10 boys, 5 girls) on a standard battery at pretest, midtest, posttest, and the two treatment groups with each other on probe measures of handwriting, spelling, and composition in each tutorial session. The treatment groups improved at a faster rate than the control group on some measures of handwriting, spelling, and composition (fluency and quality) in the standard battery, but Verbal IQ did not predict rate of improvement. Differences were found between the two treatment groups in some probe measures of writing and a motivation variable (work avoidance). Repeated-measures ANOVA was used to compare treatment groups to a non-contact control group at pretest, midtest, posttest, and follow-up. Differences between the treatment and control groups favoring the treatment groups were maintained at 6-month follow-up on some handwriting, spelling, and composition (quality) measures. Individual differences were found in learner characteristics prior to treatment and in response to the same treatment. The importance of affect and motivation as well as cognitive variables is emphasized.


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