scholarly journals Optogenetic regulation of embryo implantation in mice using photoactivatable CRISPR-Cas9

2020 ◽  
Vol 117 (46) ◽  
pp. 28579-28581
Author(s):  
Tomoka Takao ◽  
Moritoshi Sato ◽  
Tetsuo Maruyama
Keyword(s):  
Leukemia Inhibitory Factor ◽  
Gene Editing ◽  
Therapeutic Strategy ◽  
Molecular Mechanisms ◽  
Light Emitting Diode ◽  
Embryo Implantation ◽  
Light Emitting ◽  
Spatiotemporal Expression ◽  
Fertilized Egg

Embryo implantation is achieved upon successful interaction between a fertilized egg and receptive endometrium and is mediated by spatiotemporal expression of implantation-associated molecules including leukemia inhibitory factor (LIF). Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPR-Cas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility. This system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation and provides a therapeutic strategy for temporal control of reproductive functions in vivo.

scholarly journals Implantable Optrode Array for Optogenetic Modulation and Electrical Neural Recording

Micromachines ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 725
Author(s):  
Saeyeong Jeon ◽  
Youjin Lee ◽  
Daeho Ryu ◽  
Yoon Kyung Cho ◽  
Yena Lee ◽  
...  
Keyword(s):  
Light Emitting Diode ◽  
Neural Recording ◽  
Electrophysiological Recording ◽  
Light Emitting ◽  
Technological Advances ◽  
Neuroscience Research ◽  
Cell Type Specific ◽  
Novel Design

During the last decade, optogenetics has become an essential tool for neuroscience research due to its unrivaled feature of cell-type-specific neuromodulation. There have been several technological advances in light delivery devices. Among them, the combination of optogenetics and electrophysiology provides an opportunity for facilitating optogenetic approaches. In this study, a novel design of an optrode array was proposed for realizing optical modulation and electrophysiological recording. A 4 × 4 optrode array and five-channel recording electrodes were assembled as a disposable part, while a reusable part comprised an LED (light-emitting diode) source and a power line. After the characterization of the intensity of the light delivered at the fiber tips, in vivo animal experiment was performed with transgenic mice expressing channelrhodopsin, showing the effectiveness of optical activation and neural recording.

scholarly journals In vitro and in vivo Efficacy of New Blue Light Emitting Diode Phototherapy Compared to Conventional Halogen Quartz Phototherapy for Neonatal Jaundice

2005 ◽  
Vol 20 (1) ◽  
pp. 61 ◽  
Author(s):  
Yun Sil Chang ◽  
Jong Hee Hwang ◽  
Hyuk Nam Kwon ◽  
Chang Won Choi ◽  
Sun Young Ko ◽  
...  
Keyword(s):  
Blue Light ◽  
Neonatal Jaundice ◽  
Light Emitting Diode ◽  
In Vivo Efficacy ◽  
Light Emitting

Retinal damage related to high-intensity light-emitting diode exposure: An in vivo study

2021 ◽  
Author(s):  
Marcela Emílio de Araújo ◽  
Marina Bozzini Paies ◽  
Ana Beatriz Arrais ◽  
Fernando Ladd Lobo ◽  
Ruthnaldo Rodrigues Melo de Lima ◽  
...  
Keyword(s):  
High Intensity ◽  
Light Emitting Diode ◽  
In Vivo Study ◽  
Retinal Damage ◽  
Light Emitting ◽  
High Intensity Light

scholarly journals A Novel In Vivo Model of Focal Light Emitting Diode-Induced Cone-Photoreceptor Phototoxicity: Neuroprotection Afforded by Brimonidine, BDNF, PEDF or bFGF

PLoS ONE ◽  
2014 ◽  
Vol 9 (12) ◽  
pp. e113798 ◽  
Author(s):  
Arturo Ortín-Martínez ◽  
Francisco Javier Valiente-Soriano ◽  
Diego García-Ayuso ◽  
Luis Alarcón-Martínez ◽  
Manuel Jiménez-López ◽  
...  
Keyword(s):  
Light Emitting Diode ◽  
In Vivo Model ◽  
Cone Photoreceptor ◽  
Light Emitting

scholarly journals Light-emitting diode-based transcranial photoacoustic measurement of sagittal sinus oxyhemoglobin saturation in hypoxic neonatal piglets

2020 ◽  
Author(s):  
Jeeun Kang ◽  
Raymond C. Koehler ◽  
Shawn Adams ◽  
Ernest M. Graham ◽  
Emad M. Boctor
Keyword(s):  
Mean Squared Error ◽  
Light Emitting Diode ◽  
Cost Effective ◽  
Preclinical Study ◽  
Light Emitting ◽  
Neonatal Piglets ◽  
Sagittal Sinus ◽  
Photoacoustic Measurement

AbstractWe present a light-emitting diode (LED)-based transcranial photoacoustic measurement (LED-trPA) of oxyhemoglobin (HbO2) saturation at superior sagittal sinus (SSS) in hypoxic neonatal piglets. The optimal LED imaging wavelengths and frame averaging scheme were determined based on in vivo characterization of transcranial sensitivity. Based on the framework (690/850 nm with >20 frame averaging), graded hypoxia was successfully identified in neonatal piglets in vivo with less than 10.0 % of root mean squared error (RMSE). This preclinical study suggests the feasibility of a rapid, cost-effective, and safe LED-trPA monitoring of perinatal hypoxia-ischemia and prompt interventions for clinical use.

scholarly journals Spatiotemporal expression pattern of progranulin in embryo implantation and placenta formation suggests a role in cell proliferation, remodeling, and angiogenesis

Reproduction ◽  
2008 ◽  
Vol 136 (2) ◽  
pp. 247-257 ◽  
Author(s):  
Joëlle A Desmarais ◽  
Mingju Cao ◽  
Andrew Bateman ◽  
Bruce D Murphy
Keyword(s):  
Cell Proliferation ◽  
Embryo Implantation ◽  
Comparative Sequence Analysis ◽  
Placental Development ◽  
Endometrial Cells ◽  
Sequence Identity ◽  
Spatiotemporal Expression ◽  
Post Implantation

Embryo implantation in the mink is preceded by a variable but obligate period of delay in development. Under the influence of progesterone and unknown luteal factors, the mink embryo implants 11–13 days following its exit from diapause. Recent work suggests that progranulin, a growth factor and secreted glycoprotein, is involved in trophoblast proliferation, placental development and endometrial differentiation in the mouse. Using the mink model of delayed implantation and endotheliochorial placentation, we examined the spatiotemporal distribution of progranulin in trophoblast and endometrium during pre- and early post-implantation gestation in vivo. A partial sequence of the mink progranulin gene was cloned and sequenced. Comparative sequence analysis revealed that exons 1 and 2 of mink progranulin share 86.6, 82.4, and 94.9% of nucleic acid sequence identity with the human, mouse, and dog sequences respectively, and indicated that the invariable residues of the cysteine-rich motifs of progranulin are well conserved in the mink sequence. By in situ hybridization, we show that mink progranulin transcript is present in the cytotrophoblast and in epithelial and stromal endometrial cells at the site of implantation and during early placental formation. Immunohistochemistry revealed the progranulin protein to be strongly expressed in endometrial luminal and glandular epithelium around the time of implantation. In the incipient labyrinth, progranulin expression is localized to cytotrophoblasts and fetal capillaries, as well as to the hypertrophied maternal endothelial cells. This study demonstrates that high levels of progranulin expression correspond to active cell proliferation, remodeling, and angiogenesis occurring during the establishment of the placenta in the mink.

scholarly journals Histone demethylase KDM4C controls tumorigenesis of glioblastoma by epigenetically regulating p53 and c-Myc

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Dong Hoon Lee ◽  
Go Woon Kim ◽  
Jung Yoo ◽  
Sang Wu Lee ◽  
Yu Hyun Jeon ◽  
...  
Keyword(s):  
Tumor Suppressor Genes ◽  
Therapeutic Strategy ◽  
Target Gene ◽  
Molecular Mechanisms ◽  
Histone Demethylase ◽  
Wild Type ◽  
P53 Target Gene ◽  
The Stability

AbstractGlioblastoma is the most lethal brain tumor and its pathogenesis remains incompletely understood. KDM4C is a histone H3K9 demethylase that contributes to epigenetic regulation of both oncogene and tumor suppressor genes and is often overexpressed in human tumors, including glioblastoma. However, KDM4C’s roles in glioblastoma and the underlying molecular mechanisms remain unclear. Here, we show that KDM4C knockdown significantly represses proliferation and tumorigenesis of glioblastoma cells in vitro and in vivo that are rescued by overexpressing wild-type KDM4C but not a catalytic dead mutant. KDM4C protein expression is upregulated in glioblastoma, and its expression correlates with c-Myc expression. KDM4C also binds to the c-Myc promoter and induces c-Myc expression. Importantly, KDM4C suppresses the pro-apoptotic functions of p53 by demethylating p53K372me1, which is pivotal for the stability of chromatin-bound p53. Conversely, depletion or inhibition of KDM4C promotes p53 target gene expression and induces apoptosis in glioblastoma. KDM4C may serve as an oncogene through the dual functions of inactivation of p53 and activation of c-Myc in glioblastoma. Our study demonstrates KDM4C inhibition as a promising therapeutic strategy for targeting glioblastoma.

scholarly journals Towards a novel therapy against AIDS

2019 ◽  
Author(s):  
Adrien Locatelli
Keyword(s):  
Gene Editing ◽  
Therapeutic Strategy ◽  
Lentiviral Vector ◽  
Experimental Treatment ◽  
Novel Therapy ◽  
Infected People ◽  
The Moment ◽  
First Time ◽  
Puromycin Resistance Gene

AIDS is an infectious disease that kills over a million people per year. Very recently, Dash et al have for the first time reached the functional cure in HIV-infected humanized mice using CRISPR-Cas9 in combination with LASER ART, and this with a success of one third. Here, I use a theoretical approach to design a therapeutic strategy applicable to humans and different from that of Dash et al. The experimental treatment presented here includes the injection of an Env-directed integrase-defective CRISPR gene-editing lentiviral vector able to express quintuplex gRNAs plus the humanized SpCas9 and the puromycin resistance gene linked by T2A, preceded by a plasma/leukapheresis and the injection of an immunosuppressive cocktail, and followed by an in vivo positive selection. My protocol could have a major impact on HIV-infected people in the event of confirmation by a clinical trial, and it is possible that it becomes a reference treatment against AIDS, although, for the moment, it is only at the stage of hypothesis and theory.

scholarly journals Enhancing the Therapeutic Potential of Mesenchymal Stem Cells with Light-Emitting Diode: Implications and Molecular Mechanisms

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Barbara Sampaio Dias Martins Mansano ◽  
Vitor Pocani da Rocha ◽  
Ednei Luiz Antonio ◽  
Daniele Fernanda Peron ◽  
Rafael do Nascimento de Lima ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Protein Kinase ◽  
Molecular Mechanisms ◽  
Therapeutic Potential ◽  
Light Emitting Diode ◽  
Janus Kinase ◽  
Mitogen Activated Protein Kinase ◽  
Light Emitting ◽  
Report Quality

This study evaluated the effects of light-emitting diode (LED) on mesenchymal stem cells (MSCs). An electronic search was conducted in PubMed/MEDLINE, Scopus, and Web of Science database for articles published from 1980 to February 2020. Ten articles met the search criteria and were included in this review. The risk of bias was evaluated to report quality, safety, and environmental standards. MSCs were derived from adipose tissue, bone marrow, dental pulp, gingiva, and umbilical cord. Protocols for cellular irradiation used red and blue light spectrum with variations of the parameters. The LED has been shown to induce greater cellular viability, proliferation, differentiation, and secretion of growth factors. The set of information available leads to proposing a complex signaling cascade for the action of photobiomodulation, including angiogenic factors, singlet oxygen, mitogen-activated protein kinase/extracellular signal-regulated protein kinase, Janus kinase/signal transducer, and reactive oxygen species. In conclusion, although our results suggest that LED can boost MSCs, a nonuniformity in the experimental protocol, bias, and the limited number of studies reduces the power of systematic review. Further research is essential to find the optimal LED irradiation parameters to boost MSCs function and evaluate its impact in the clinical setting.

Sign in / Sign up

Export Citation Format

Share Document