Effects of desiccation on the subcellular matrix of the embryonic axes of Quercus robur.

AbstractFruit and seed development in Quercus robur L. were studied on a single tree over five consecutive seasons. Patterns of growth in the cotyledons and embryonic axes differed between years and resulted in seeds of very different sizes. Moisture content at shedding also differed between years, and late-shed seeds had lower moisture contents than early-shed seeds. Moisture content at shedding was negatively correlated with desiccation tolerance. Seed development in Q. robur therefore appeared indeterminate and did not end in a period of rapid desiccation.Sensitivity to desiccation in Q. robur was not due to an inability to accumulate dehydrin proteins, ABA or soluble sugars, substances that have been linked with the acquisition of desiccation tolerance in orthodox seeds. There were great similarities between several aspects of Q. robur seed development and that of orthodox seeds before the latter entered the terminal phase of rapid desiccation. This pattern of seed development contrasted with that reported for the highly desiccation-sensitive seeds of Avicennia marina.

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Free radical activity and loss of viability during drying of desiccation-sensitive tree seeds

Proceedings of the Royal Society of Edinburgh Section B Biological Sciences ◽

10.1017/s0269727000014196 ◽

1994 ◽

Vol 102 ◽

pp. 257-260 ◽

Cited By ~ 6

Author(s):

W. E. Finch-Savage ◽

G. A. F. Hendry ◽

N. M. Atherton

Keyword(s):

Lipid Peroxidation ◽

Free Radical ◽

Quercus Robur ◽

Moisture Stress ◽

Recalcitrant Seeds ◽

Embryonic Axes ◽

Stable Free Radical ◽

Tree Seeds ◽

Free Radical Activity ◽

Oxidative Attack

SynopsisViability loss during desiccation in the recalcitrant seeds of Castanea saliva, Aesculus hippocastanum and Quercus robur was accompanied by increased lipid peroxidation and build up of a stable free radical within their embryonic axes. We argue that the accumulation of the free radical marks the termination of a series of destructive events following the initiation of oxidative attack during moisture stress in recalcitrant seeds.

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Physiological aspects of recalcitrance in embryonic axes of Quercus robur L.

Seed Science Research ◽

10.1017/s0960258500001409 ◽

1992 ◽

Vol 2 (4) ◽

pp. 215-221 ◽

Cited By ~ 14

Author(s):

K. M. Poulsen ◽

E. N. Eriksen

Keyword(s):

Water Potential ◽

Quercus Robur ◽

Fold Increase ◽

Proline Content ◽

Short Exposure ◽

Embryonic Axes ◽

Vegetative Tissue ◽

Water Potentials ◽

Orthodox Seeds ◽

Peak Value

AbstractThe sorption isotherm for excised embryonic axes of recalcitrant (i.e. desiccation-sensitive) Quercus robur L. acorns was determined to find the relation between moisture content and water potential. Subsequently, physiological studies on the effect of desiccating the axes to a range of water potentials were undertaken. The respiratory capacity declined steeply after short exposure to water potentials from −5 to −30 MPa. The leachate conductivity increased significantly after exposure to −5 MPa and rose steeply after exposure to between −12 and −40 MPa. Axes were equilibrated at different relative humidities and the proline content showed a 15-fold increase with a peak value at −10 MPa. It was concluded that the critical water potential for initiation of damage was −5 MPa, and that axes accumulated proline as a response to desiccation stress. The embryonic axes from Q. robur behave more like typical vegetative tissue of angiosperms than like orthodox seeds.

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Cryopreservation changes the DNA methylation of embryonic axes of Quercus robur and Fagus sylvatica seeds during in vitro culture

Trees ◽

10.1007/s00468-016-1416-3 ◽

2016 ◽

Vol 30 (5) ◽

pp. 1831-1841 ◽

Cited By ~ 4

Author(s):

Katarzyna Nuc ◽

Małgorzata Marszałek ◽

Paweł M. Pukacki

Keyword(s):

Dna Methylation ◽

In Vitro Culture ◽

Fagus Sylvatica ◽

Quercus Robur ◽

Embryonic Axes

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Preservation of Quercus robur L. Embryonic Axes in Liquid Nitrogen

Basic and Applied Aspects of Seed Biology - Current Plant Science and Biotechnology in Agriculture ◽

10.1007/978-94-011-5716-2_83 ◽

1997 ◽

pp. 765-769 ◽

Cited By ~ 2

Author(s):

P. Chmielarz

Keyword(s):

Liquid Nitrogen ◽

Quercus Robur ◽

Embryonic Axes

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Effect of short-chain fatty acids on the ethylene pathway in embryonic axes of Cicer arietinum during germination

Physiologia Plantarum ◽

10.1034/j.1399-3054.1994.920413.x ◽

1994 ◽

Vol 92 (4) ◽

pp. 629-635 ◽

Cited By ~ 1

Author(s):

Mercedes Gallardo ◽

Paloma Munoz De Rueda ◽

Angel Jesus Matilla ◽

Isabel Maria Sanchez-Calle

Keyword(s):

Fatty Acids ◽

Cicer Arietinum ◽

Short Chain Fatty Acids ◽

Short Chain ◽

Embryonic Axes ◽

Chain Fatty Acids

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A comparative study of the antibacterial activity of Quercus robur (Ethanolic extract) and Zinc oxide nanoparticles on Salmonella (In vitro)

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v9ispl1.1397 ◽

2018 ◽

Vol 9 (SPL1) ◽

Author(s):

Hams H. H. Alfattli ◽

Ghufran Zuhair Jiber ◽

Ghaidaa Gatea Abbass

Keyword(s):

Zinc Oxide ◽

Antibacterial Activity ◽

Zinc Oxide Nanoparticles ◽

Quercus Robur ◽

Ethanolic Extract ◽

Inhibitory Effect ◽

Significant Inhibition ◽

Oxide Nanoparticles ◽

Inhibition Zones

This study which designed to evaluate the inhibitory effect of Ethanolic extract of (Quercusrobur) and Zinc oxide nanoparticles on the growth of one genus of enterobacteriacae (Salmonella). In vitro. For this purpose graduate concentrates for plant extract (50, 100, 200, 400 )mg/ml which prepared and compared with Zinc oxide nanoparticles of different concentration (2, 1, 0.5, 0.25) μg/ml,and examined. The result showed that the studied medicinal plant has antibacterial activity against this bacteria which used. The result showed that the plant has good activity in decrease the growth of this bacteria. The results of the study also showed that the nano-ZnO has very effective antibacterial action against the studied bacteria which was Salmonella,nanoparticles concentrations lead to increasing in the inhibition zones of tested bacterial growth. We also study the effect of three antibiotics Lomefloxacin (LOM), Ciprofloxacin (SIP) and Rifampin (RA) and the result showed,in a comparison within the tested bacteria,Salmonella had a significant inhibition increase in Lomefloxacin ; the ciprofloxacin showed effect on tested bacteria. However,Rifampin does not show any effect on tested bacteria.

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The In vitro Evaluation of the Antimicrobial Activity of Quercus robur L. Methanolic and Aqueous Leaves’ Extracts, from the Algerian High Plateaus Against some Uropathogenic Microbial Strains

Phytothérapie ◽

10.3166/phyto-2019-0133 ◽

2019 ◽

Vol 18 (5) ◽

pp. 262-274

Author(s):

E. Benyagoub ◽

N. Nabbou ◽

S. Boukhalkhel ◽

I. Dehini

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Enterococcus Faecalis ◽

Fusidic Acid ◽

Quercus Robur ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Microbial Strains

The medicinal value of the plants is due to their chemical components that bring a definite physiological action on the human body to prevent the diseases. In this work, we investigated the antimicrobial activity of leaves’ extracts of Quercus robur L., collected from the Algerian upper highlands, on ten bacterial strains and one fungal strain known to be pathogenic. First, we performed a qualitative phytochemical analysis, and second, antimicrobial activity tests performed by agar diffusion method (disc and well) with the determination of MIC by broth macro-dilution method. Given the results, it appears that obtained macerates of Quercus robur L. were rich in bioactive phytoconstituents such as alkaloids, anthraquinones, saponins, tannins, and other components. The yield of aqueous and methanolic macerates of leaves was 8.5 ± 1.41 and 22.4 ± 4.36%, respectively. The bacterial resistance was relatively important to several antibiotics, namely, ampicillin, amoxicillin + clavulanic acid for strains of Escherichia coli and Salmonella sp. However, Staphylococcus aureus strains were resistant to fusidic acid, penicillin, and oxacillin; while Enterococcus faecalis was resistant to fusidic acid, penicillin, oxacillin, and ticarcillin. The antibacterial activity of the macerates toward tested microbial strains showed that the aqueous and methanolic macerates of the leaves were proportional to the tested concentration and active not only against Gram-positive and Gram-negative bacteria but also on the fungal species Candida albicans. The estimated MIC for Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus was in the order of 10 mg/mL, which seems more effective than toward Salmonella sp., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans which were in the order of 30 mg/mL. These preliminary results confirm that the part of the studied plant had a very good antimicrobial activity that was proportional to the serial concentrations of the tested extracts.

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