scholarly journals Glucose and protein metabolism during late pregnancy in triplet-bearing ewes given fresh forages ad lib.

1985 ◽  
Vol 54 (2) ◽  
pp. 521-533 ◽  
Author(s):  
T. N. Barry ◽  
T. R. Manley

1. Ewes of the Booroola x Romney genotype carrying triplet lambs were given fresh forages ad lib. in late pregnancy. In Expt 1, groups of three ewes were given kale (Brassica oleracea), perennial ryegrass (Loliumperenne) or perennial ryegrass (0.75)–barley (0.25). In Expt 2, groups of two or three ewes were given fresh perennial ryegrass and infused into the abomasum with iso-energetic quantities of casein and glucose in a 2 x 2 factorial arrangement. Post-lambing ewe live weights were 40–50 kg. Glucose irreversible loss (GIL) was determined from dilution of D-[U-14C]glucose.2. For ewes given kale, perennial ryegrass and perennial ryegrass—barley in Expt 1, mean metabolizable energy (ME) intakes were 0.50, 0.82 and 0.83 MJ/kg live Weight0.75 per d, GIL was 112, 142 and 157 g/d, and mean birth weight 2.22, 3.05 and 2.95 kg/lamb.3. In Expt 2, infusion of glucose, casein, and glucose+casein depressed herbage ME intake respectively by 1.6, 0.9 and 0.3 times the amount of ME infused. GIL (185–325 g/d) was increased by 800 and 350 g respectively for each kg of glucose or casein infused. Casein infusion increased calculated amino acid absorption from 0.18 to 0.36 of ME, increased wool growth and increased calculated maternal N balance. Birth weight was unaffected by nutritional treatment and averaged 3.29 kg/lamb.4. When values from both experiments were combined, birth weight was related to GIL by a hyperbolic relation, with maximum predicted birth weight being 4.1 kg/lamb. It was postulated that this value was never attained in practice, due to uterine expansion being restricted by the low maternal body size. Marked decreases in birth weight occurred when GIL decreased below 173 g/d.5. It was calculated that ewes in all treatment groups were in negative energy balance, and that glucose supplied by the kale and unsupplemented ryegrass diets were respectively below and equal to calculated conceptus uptakes of glucose necessary to maintain growth of triplet fetuses. It was further calculated that amino acid requirements of triplet-bearing ewes in late pregnancy were likely to exceed substantially net absorption from digestion of fresh forage diets, and that maternal tissues go into negative N balance to ensure fetal growth, thus explaining the lack of response to abomasal casein infusion.

Author(s):  
NG Das ◽  
NR Sarker ◽  
KS Huque ◽  
MAI Talukdar

The study was conducted with the objective to investigate prenatal and postnatal performances of Red Chittagong Cattle (RCC) cows in subsistence feeding and nutrition compared to those in improved feeding system. Twenty RCC cows with average initial live weight of 204.6 (±29) kg at their 2nd or 3rd parity and at about seven months of pregnancy were divided into two equal groups and assigned to two dietary treatments: one group was fed as per farmers’ choice, while another was fed 25% higher metabolizable energy (ME) and crude protein (CP) than requirements through concentrates, according to ARC (2009). Cows in farmers’ diet during last 50 days of pregnancy fed lower ME and CP (42.4 MJ and 445 g, respectively) than their daily requirements (43.5 MJ and 519 g, respectively), resulted in lower birth weight of calves compared to improved feeding (13.36 and 15.12 kg, respectively) where daily ME and CP intake was 57.77 MJ and 603 g. In first 60 days of lactation, cows under farmers’ diet fed required amount of ME and CP (44.83 MJ and 471g, respectively) compared to requirement (42 MJ and 388g, respectively). However, cows in improved diet fed 1.28 and 1.42 times higher ME and CP (52.92 MJ and 563 g) than requirements, and produced higher milk (1.97 and 2.50 L/d; P<0.05) and daily gain (122 and 274 g/d; P<0.01), compared to that of farmers’ diet cows. Therefore, it was concluded that diet of RCC cows in late pregnancy may be supplemented with CP in order to produce higher birth weight of calves. In case of lactating cows, a cost effective supplementation of concentrate mixture for increasing milk production and daily gain may be investigated by doing further research. Bangladesh J. of Livestock Res. 21-25: 159-167, 2018


1979 ◽  
Vol 29 (3) ◽  
pp. 393-399 ◽  
Author(s):  
A. M. Khalaf ◽  
D. L. Doxey ◽  
J. T. Baxter ◽  
W. J. M. Black ◽  
J. FitzSimons ◽  
...  

ABSTRACTOne hundred and thirty-nine Scottish Half bred ewes were studied during the last 8 weeks of pregnancy, through parturition and early lactation. They were divided, on the basis of their metabolizable energy intake during this period, into low (483 MJ/ewe; L), medium (742 MJ/ewe; M) or high (974 MJ/ewe; H) feeding groups.Ewe weight change (from mating to 12 h post lambing) was directly related to nutritional level and the number of lambs born, e.g. L ewes with triplets lost a mean 13·8 kg, while H ewes with single lambs gained 14·3 kg.Lamb birth weight and perinatal lamb mortality levels were affected by ewe nutrition and litter size. L twins weighed 19% less at birth than H twins; L triplets weighed 26% less than H triplets. The mortality rate of L twins was 23% greater than M twins; L triplets exceeded the H triplet mortality rate by 87%.Ewe energy feeding during late pregnancy affected the mean daily weight gain of lambs for at least 3 weeks after birth. H single, twin and triplet lambs grew 12%, 15% and 16% faster than M lambs and 19%, 31 % and 31 % faster than L lambs respectively.The H group produced 33 % more lamb live weight at 3 weeks of age for every lamb born than did the L group.Lamb serum immunoglobulin levels were related to litter size but did not reflect the differences in ewe feeding during late pregnancy.


1981 ◽  
Vol 97 (3) ◽  
pp. 723-729 ◽  
Author(s):  
A. J. F. Russel ◽  
Janet Z. Foot ◽  
I. R. White ◽  
G. J. Davies

SUMMARYForty-eight approximately 18-month-old Scottish Blackface ewes were used to study the effects of two levels of nutrition during mid-pregnancy (30–98 days of gestation) on the birth weight of lambs from ewes varying in weight, size and condition at first mating. The mean live weight (42–4 kg), size index (31–9) and condition score (2–4) at mating of the 26 ewes from flock A were all less than those of the 22 ewes from flock B (54–5, 39–4 and 2–9 respectively). Mean intakes of the low and high nutritional treatment ewes during mid-pregnancy were 10–6 and 22–0 g/kg/day respectively of a pelleted diet supplying 8–81 MJ metabolizable energy and 125–5 g crude protein/kg. These intakes produced estimated changes in net maternal weight of approximately – 5 and 0 kg respectively.Mean lamb birth weights from ewes on the low and high nutritional treatments were:flock A, 3–32 and 3–83 kg; flock B, 4–96 and 4–23 kg respectively. Analyses showed intake during mid-pregnancy to have a positive effect on lamb birth weight in the flock A ewes, and a negative effect in flock B ewes. Mating weight accounted for 78% of the variance in birth weight in the low nutritional treatment ewes but had little effect in those on the higher level of feeding.The practical implications of the results are discussed in relation to levels of juvenile nutrition.


2002 ◽  
Vol 75 (3) ◽  
pp. 415-426 ◽  
Author(s):  
I. Fernández-Fígares ◽  
R. Nieto ◽  
the late C. Prieto ◽  
J. F. Aguilera

AbstractAn experiment was carried out in growing chickens to study the effect of supplementation of a semi-synthetic diet containing soya-bean meal as the sole protein source with DL-methionine, to improve its biological value, on the excretion of endogenous protein and amino acids measured in lower ileum and total tract using traditional methods. Thirty-two White Rock male broilers (10 days old) were randomly divided into eight groups each of four birds, of similar body weight (mean live weight: 142·8 (s.e. 0·68) g), and individually housed in metabolism cages. Following a paired-feeding design based on metabolic body weight (kgM0·75), each group of birds was given, for an experimental period of 20 days, each of four levels of protein (60, 120, 180 or 240 g/kg; 5 days each) in two groups of isoenergetic (14·5 kJ metabolizable energy per g dry matter) and semi-synthetic diets based on soya-bean meal, either not supplemented or supplemented with 2 g/kg DL-methionine (diets S and SM, respectively). After 3 days of each treatment excreta were collected for 48 h, frozen and stored at –20ºC. At the end of the fourth treatment three chickens of each group were killed and their lower ileal contents collected. The remaining chick of each treatment was fasted for 24 h and given a protein-free diet for 8 days and excreta were collected for the last 4 days. Then (day 39 of age), chickens were killed and lower ileum contents removed and stored at –20ºC. Samples of excreta and lower ileum contents were subjected to nitrogen (N) analysis by Kjeldahl procedure and amino acid (AA) analysis by high-performance liquid chromatography. Supplementation with DL-methionine of the soya-bean meal-based diets halved total tract endogenous AA losses. Regression analysis produced a higher estimation of ileal and faecal endogenous AA excretion than feeding a protein-free diet. Endogenous AA excretion determined in the lower ileum was higher than in excreta no matter which estimation procedure was utilized. In conclusion, supplementation of dietary protein with the first limiting AA to improve its protein quality, causes an important drop in endogenous AA losses, that may have an important effect on the N economy and energy requirements in poultry. The use of regression analysis on excreta data where graded amounts of protein are given to growing chickens, seems a suitable method for determining endogenous AA losses provided that good quality proteins are used.


1986 ◽  
Vol 43 (1) ◽  
pp. 155-165 ◽  
Author(s):  
H. T. Yen ◽  
D. J. A. Cole ◽  
D. Lewis

ABSTRACTThe response to dietary lysine when provided as part of an ideal protein was examined in pigs over the range of live weight from 50 to 90 kg. Ninety-six pigs, 32 trios of littermate boars, castrated males and gilts were allocated to eight diets with lysine concentrations of 5·6, 6·2, 7·3, 8·3, 9·3, 10·3, 11·4 and 12·4 g/kg diet and corresponding concentrations of crude protein (CP) from 90 to 186 g/kg diet (all having a digestible energy of 13·38 MJ/kg). Pigs were given food on a restricted scale once daily. With increasing lysine concentration, there was a linear response (followed by a plateau) for growth and carcass characteristics up to dietary lysine concentrations of 91, 7·2 and 8·4 g/kg diet and CP concentrations of 142, 116 and 132 g/kg diet with daily lysine intakes of 22·9, 18·6 and 21·2 g and CP intakes of 357, 290 and 331 g for boars, castrated males and gilts respectively.


2020 ◽  
Vol 42 ◽  
pp. e49022
Author(s):  
Wagner Dias Coelho de Oliveira ◽  
Tairon Pannunzio Dias e Silva ◽  
Leílson Rocha Bezerra ◽  
Marcos Jácome de Araújo ◽  
Ricardo Loiola Edvan ◽  
...  

The aim of this study was to evaluate the protein energetic, enzymatic and mineral profile of Nellore cows during the pregnancy, parturition and postpartum. Fifteen multiparous cows with 4 ± 1 years of age and live weight of 400 ± 50 kg were used at different stages (non-pregnant, in the initial, middle and late pregnancy, at birth, one day postpartum, 30 and 60 days postpartum). Blood collections were performed every 30 days and assayed for the following blood biomarkers: Protein (total proteins, albumin, urea and creatinine), energetic (cholesterol, triglycerides, glucose and beta hydroxybutyrate), mineral (calcium, phosphorus, magnesium) and enzymatic (alkaline phosphatase and aspartate aminotransferase). Calcium had the lowest concentrations (p < 0.05) in the initial pregnancy, while phosphorus had the highest concentration at parturition (p < 0.05). Triglycerides, glucose and beta hydroxybutyrate were influenced by the stages of pregnancy, reducing in the late pregnancy and at parturition. Glucose had a reduction in the late pregnancy and elevation in the postpartum. Beta hydroxybutyrate showed increase at the late pregnancy. Although lipomobilization occurred in the phases of higher metabolic demands in the attempt to maintain homeostatic conditions. Nellore cows did not present negative energy balance in the late pregnancy period, maintaining normal variation in blood markers throughout the experimental period.


2007 ◽  
Vol 146 (2) ◽  
pp. 163-170 ◽  
Author(s):  
E. KEBREAB ◽  
J. FRANCE ◽  
H. DARMANI KUHI ◽  
S. LOPEZ

SUMMARYThe results from three types of study with broilers, namely nitrogen (N) balance, bioassays and growth experiments, provided the data used herein. Sets of data on N balance and protein accretion (bioassay studies) were used to assess the ability of the monomolecular equation to describe the relationship between (i) N balance and amino acid (AA) intake and (ii) protein accretion and AA intake. The model estimated the levels of isoleucine, lysine, valine, threonine, methionine, total sulphur AAs and tryptophan resulting in zero balance to be 58, 59, 80, 96, 23, 85 and 32 mg/kg live weight (LW)/day, respectively. These estimates show good agreement with those obtained in previous studies. For the growth experiments, four models, specifically re-parameterized for analysing energy balance data, were evaluated for their ability to determine crude protein (CP) intake at maintenance and efficiency of utilization of CP intake for producing gain. They were: a straight line, two equations representing diminishing returns behaviour (monomolecular and rectangular hyperbola) and one equation describing smooth sigmoidal behaviour with a fixed point of inflexion (Gompertz). The estimates of CP requirement for maintenance and efficiency of utilization of CP intake for producing gain varied from 5·4 to 5·9 g/kg LW/day and 0·60 to 0·76, respectively, depending on the models.


1978 ◽  
Vol 26 (3) ◽  
pp. 233-243 ◽  
Author(s):  
T. W. Griffiths

AbstractTwenty-seven castrate male cattle were used in two nutritional balance and slaughter experiments to measure the effects of increasing levels of dietary protein (9 to 15% in the dry matter) at two levels of feeding (approximately 16 and 22 MJ metabolizable energy (ME)/100 kg live weight (LW) per day) on digestibility, nitrogen (N) balance and carcass growth and composition over the LW range 130 to 390 kg.The higher level of feeding increased LW gain and carcass gain (CG) but higher protein intake increased LW gain and CG only at the higher feeding level as a result of its favourable effect on digestibility. N balance tended to overestimate carcass N retention at higher levels of protein intake.The higher level of feeding increased the separable fat and total fat in the carcass. Increased protein intake had no effect on the lean meat content but increased the percentage protein in the edible portion of the carcass. LW gain, CG and carcass energy deposition were related to ME intake only but N balance and carcass protein gain were related to both ME and digestible crude protein intake.


1988 ◽  
Vol 46 (2) ◽  
pp. 283-290 ◽  
Author(s):  
R. G. Campbell ◽  
M. R. Taverner ◽  
C. J. Rayner

AbstractForty-three entire males were used to determine the pig's tissue requirements for protein and amino acids from 8·0 to 20·0 kg, and provide information on the capacity of diets formulated with conventional ingredients to contain the same levels and balances of amino acids as ideal protein to supply these nutrients. Seven diets with similar digestible energy (15·9 MJ digestible energy (DE) per kg) and crude protein concentrations from 119 to 232 g/kg (8·7 to 17·3 g lysine per kg) were offered ad libitum between 8·0 and 200 kg live weight. The rate of protein deposition was determined by comparative slaughter. The composition of the protein deposited in the whole empty body was determined from amino acid analyses of pigs killed at 8·0 kg and from the two extreme dietary treatments at 20·0 kg. Growth performance and the rates at which protein and lysine were deposited in the empty body increased linearly with increasing dietary protein concentration up to 187 g/kg and remained relatively constant thereafter. The corresponding dietary protein and lysine intakes required to support maximal protein accretion were 178 g/day (11·7 g/MJ DE) and 13·0 g/day (0·84 g/MJ DE) respectively. Based on the maximal deposition rates for protein (91·8 g/day), and lysine (5·96 g/day) and endogenous protein loss (77middot;6 g/day) estimated from the linear component of the relationship determined between protein deposition and apparent digestible protein intake, the pig's tissue requirements for protein and lysine were only 99·4 g/day (6·5 g/MJ DE) and 6·46 g/day (0·43 g/MJ DE) respectively. This disparity between the pig's tissue protein and amino acid requirements and the dietary levels needed to support these was associated with the fact that the apparent digestibility and biological value of the dietary protein were 0·92 and 0·602 respectively. Apart from small differences in the lysine content of body protein and the methionine: lysine ratio, the average amino acid composition of pigs killed at 8·0 kg, and from the diet of highest protein concentration at 20 kg, was similar to that of ideal protein, indicating that the low utilizability of dietary protein for tissue growth and maintenance was probably associated with low amino acid digestibility and/or availability. The implications of the results with respect to expression of the growing pig's requirements for protein and amino acids are discussed.


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