A combination Prussian blue – hematoxylin and eosin staining technique for identification of iron and other histological features

✓ A monoclonal antibody against the surface marker IOT-10 of natural killer (NK) cells was used to investigate the presence of these cells in a series of 25 glioblastomas. In 40% of the tumors, IOT-10-positive NK cells were found in small numbers scattered among the tumor cells. The presence of IOT-10-positive NK cells was not related to the degree of lymphocytic infiltration in the tumor as demonstrated by hematoxylin and eosin staining, nor did it appear to influence the survival time of the patients studied.

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Complement C9 expression is associated with damaged myocardial cells in pediatric sudden death cases of fulminant myocarditis

Egyptian Journal of Forensic Sciences ◽

10.1186/s41935-020-00211-5 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Akari Takaya Uno ◽

Masahito Hitosugi ◽

Mami Nakamura ◽

Tomoyuki Nakanishi ◽

Takahiro Mima ◽

...

Keyword(s):

Sudden Death ◽

Acute Myocarditis ◽

Lymphocytic Infiltration ◽

Pericardial Fluid ◽

Sirtuin 1 ◽

Fulminant Myocarditis ◽

Myocardial Cells ◽

Hematoxylin And Eosin ◽

Hematoxylin And Eosin Staining ◽

Complement C9

Abstract Background Because disease progression is so fast in sudden death of acute fulminant myocarditis, damage of myocardial cells is not evident in routine hematoxylin and eosin staining. To understand damage to myocardial cells and the mechanism of sudden death, immunohistochemical staining was performed for two forensic autopsy cases. Case presentation The patients were a healthy 5-year-old girl and 8-year-old boy. They suddenly died within 2 days of appearance of flu-like symptoms. An autopsy showed accumulation of yellowish-clear pericardial fluid containing fibrin deposits, fluid blood in the heart, and congestion of visceral organs. Histologically, minor necrosis or degeneration of myocardial cells with mainly lymphocytic infiltration was observed sometimes in tissue sections. Immunohistochemically, positive complement C9 staining and negative sirtuin 1 staining were found. These findings suggested wide damage of myocardial cells, even in regions with no marked changes in myocardial cells with hematoxylin and eosin staining. These areas corresponded to those with strong accumulation of lymphocytes. Conclusions Immunohistochemistry for complement C9 and sirtuin 1 might become a new tool for evaluating damage of myocardial cells of fulminant acute myocarditis.

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Suicide by intoxication with iron (III) chloride

Forensic Toxicology ◽

10.1007/s11419-021-00579-6 ◽

2021 ◽

Author(s):

Sławomir Majdanik ◽

Barbara Potocka-Banaś ◽

Sebastian Glowinski ◽

Krzysztof Borowiak

Keyword(s):

Prussian Blue ◽

Chloride Ions ◽

Iron Chloride ◽

Histopathological Analysis ◽

Fatal Intoxication ◽

Internal Organs ◽

Specific Staining ◽

Iron Supplements ◽

Staining Protocol ◽

Hematoxylin And Eosin

Abstract Purpose Cases of iron intoxication are not very often encountered in toxicology practice, and most of those reported concern accidental intoxications with iron supplements in young children. The paper presents a rare case of a suicide by intoxication in an adult woman who ingested a solution of iron (III) chloride. Methods A forensic was at the Department of Forensic Medicine, PMU in Szczecin. Toxicology tests of blood sampled from the deceased were performed using a 644 CIBA CORNING ion selective analyzer and proprietary reagent kits. Histopathological was with the use of the standard staining protocol (hematoxylin and eosin) and staining specific for iron (Prussian blue). Results Autopsy revealed a distinct yellow discolouration and thrombotic necrosis of the oral mucosa and almost the whole gastrointestinal tract, as well as similar changes in the adjacent internal organs. Considerably high levels of iron and chloride ions were detected in specimens of internal organs preserved during autopsy. Histopathological analysis performed with the use of staining specific for iron (Prussian blue) also confirmed the presence of iron in the examined tissues, especially in the intestines and liver. Conclusions Considering the above findings, it was concluded in the forensic report that the death of the woman was caused by the ingestion of iron chloride. The reported case of fatal intoxication is one of the few described in the literature, and its course implies that in the case of initially diagnosed intoxication with corrosive compounds, the possibility of using metal-containing poison should also be considered in the differential diagnosis. In addition to routine toxicological tests performed in fatal cases we also draw attention to the possibility of using specific staining protocols for microscopic specimens.

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Therapeutic Efficacies of Artemisia capillaris Extract Cream Formulation in Imiquimod-Induced Psoriasis Models

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/3610494 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Song Yi Lee ◽

Suyeong Nam ◽

Sungyun Kim ◽

Ja Seong Koo ◽

In Kee Hong ◽

...

Keyword(s):

Skin Lesions ◽

The Other ◽

Epidermal Thickness ◽

Artemisia Capillaris ◽

Cream Formulation ◽

Cumulative Score ◽

Intracellular Adhesion Molecule ◽

Psoriasis Therapy ◽

Hematoxylin And Eosin ◽

Hematoxylin And Eosin Staining

A cream formulation containing Artemisia capillaris (AC) extract (ACE) was developed for psoriasis therapy. Although ACE can be dissolved in organic solvents, its topical application is restricted because of toxicities. Therefore, a cream formulation was developed for the convenient and safe local application of ACE on skin lesions. The antipsoriatic properties of the ACE cream were evaluated using an imiquimod- (IMQ-) induced psoriasis-like mouse model. In psoriasis-like mouse models, the cumulative score (redness, thickness, and scaling) of the IMQ + ACE cream group was significantly lower than those of the other groups on day 4 (p < 0.05). The results of the hematoxylin and eosin staining of skin tissues revealed that the epidermal thickness value of the IMQ + ACE cream group was significantly lower than those of the other experimental groups (p < 0.05). The expression level of intracellular adhesion molecule-1 (ICAM-1), which indicates the leukocyte infiltration into the skin and subsequent interactions with keratinocytes, was also lower in the IMQ + ACE cream group than in the IMQ group. These results indicate that ACE cream formulation could be used safely and conveniently for psoriasis treatment.

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Beclin 1, LC3, and p62 expression in paraquat-induced pulmonary fibrosis

Human & Experimental Toxicology ◽

10.1177/0960327119842633 ◽

2019 ◽

Vol 38 (7) ◽

pp. 794-802 ◽

Cited By ~ 4

Author(s):

G Xu ◽

X Wang ◽

H Yu ◽

C Wang ◽

Y Liu ◽

...

Keyword(s):

Pulmonary Fibrosis ◽

Light Chain ◽

Lung Fibrosis ◽

Beclin 1 ◽

Related Protein ◽

Protein Expressions ◽

Hematoxylin And Eosin ◽

Hematoxylin And Eosin Staining ◽

Related Proteins

Paraquat (PQ) is a highly toxic herbicide to humans. Pulmonary fibrosis is one of the most typical features of PQ poisoning, which develops from several days to weeks after ingestion. However, the mechanism of fibrosis is still unclear. In this study, we aimed to determine expressions of autophagy-related markers Beclin 1, microtubule-associated protein light chain 3 (LC3), and p62 in PQ-poisoned lungs and to explore the role of autophagy in pulmonary fibrosis induced by PQ. We detected markers of lung fibrosis and expressions of autophagy-related protein in the specimens from eight fatal cases of PQ poisoning by hematoxylin and eosin staining, Masson’s trichrome staining, and immunohistochemistry. Based on the staining results of lung fibrosis, these cases were divided into two groups, fibrosis and non-fibrosis groups. The correlation between autophagy protein expressions and pulmonary fibrosis was examined. The results demonstrated that the autophagy-related proteins were significantly expressed in fibrosis group compared with the non-fibrosis group. There was a significantly positive correlation between these protein expressions and severity of lung fibrosis. In conclusion, autophagy dysfunction may be involved in lung fibrogenesis caused by PQ poisoning. This may be a promising clue for understanding the molecular mechanism underlying PQ-induced lung fibrosis and provide evidence for treating fibrosis by regulating the level of autophagy.

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Use of Deep Learning to Develop and Analyze Computational Hematoxylin and Eosin Staining of Prostate Core Biopsy Images for Tumor Diagnosis

JAMA Network Open ◽

10.1001/jamanetworkopen.2020.5111 ◽

2020 ◽

Vol 3 (5) ◽

pp. e205111

Author(s):

Aman Rana ◽

Alarice Lowe ◽

Marie Lithgow ◽

Katharine Horback ◽

Tyler Janovitz ◽

...

Keyword(s):

Deep Learning ◽

Core Biopsy ◽

Tumor Diagnosis ◽

Hematoxylin And Eosin ◽

Hematoxylin And Eosin Staining

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Morphometric Evaluation of Spermatogenic Cells and Seminiferous Tubules and Exploration of Luteinizing Hormone Beta Polypeptide in Testis of Datong Yak

Animals ◽

10.3390/ani10010066 ◽

2019 ◽

Vol 10 (1) ◽

pp. 66 ◽

Cited By ~ 1

Author(s):

Qudratullah Kalwar ◽

Min Chu ◽

Anum Ali Ahmad ◽

Xuezhi Ding ◽

Xiaoyun Wu ◽

...

Keyword(s):

Quantitative Pcr ◽

Developmental Stages ◽

Seminiferous Tubules ◽

Spermatogenic Cells ◽

Sex Development ◽

Significant Difference ◽

Hematoxylin And Eosin ◽

Hematoxylin And Eosin Staining ◽

Morphometric Evaluation ◽

Semi Quantitative Pcr

Histological examination of testes is essential for understanding infertility, sex development, and growth. Therefore, to understand the histomorphology of testes at different developmental stages, we performed hematoxylin and eosin staining of Yak testis. Our results revealed that the diameters of spermatogenic cells and their nuclei were significantly larger (p < 0.05) in the testis at six years compared to at six and 18 months. No significant difference was noted between 30 months and six years. The study was designed to compare the expression profile of LHB in Datong yak. The expression pattern of LHB was explored using quantitative PCR, semi-quantitative PCR, molecular bioinformatic, and Western blot analysis. Our observations indicated that expression of LHB was significantly higher (p < 0.05) in the testis of Datong yak. Western blotting indicated that the molecular mass of LHB protein was 16 kDa in yak. The protein encoded by yak LHB included conserved cysteine-knot domain regions. The high expression of LHB in testis indicated that LHB may be vital for the development of male gonads and the fertility of Datong yak.

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Mucormycosis of the hard palate masquerading as carcinoma

Clinics and Practice ◽

10.4081/cp.2012.e28 ◽

2012 ◽

Vol 2 (1) ◽

pp. 28 ◽

Cited By ~ 3

Author(s):

Bhari Sharanesha Manjunatha ◽

Nagarajappa Das ◽

Rakesh V. Sutariya ◽

Tanveer Ahmed

Keyword(s):

Clinical Presentation ◽

Fungal Infections ◽

Survival Rates ◽

Positive Culture ◽

Staining Technique ◽

Necrotic Bone ◽

Chronic Ulcers ◽

Hematoxylin And Eosin ◽

Clinical Differential Diagnosis ◽

Hematoxylin And Eosin Stain

A growing number of medically compromised patients are encountered by dentists in their practices. Opportunistic fungal infections such as mucormycosis usually occur in immunocompromised patients but can infect healthy individuals as well. Mucormycosis is an acute opportunistic, uncommon, frequently fatal fungal infection, caused by a saprophytic fungus that belongs to the class of phycomycetes. Among the clinical differential diagnosis we can consider squamous cell carcinoma. Such cases present as chronic ulcers with raised margins causing exposure of underlying bone. There is a close histopathological resemblance between mucormycosis and aspergillosis. Microscopically, aspergillosis has septate branching hyphae, which can be distinguished from mucormycotic hyphae by a smaller width and prominent acute angulations of branching hyphae. A definitive diagnosis of mucormycosis can be made by tissue biopsy that identifies the characteristic hyphae, by positive culture or both. The culture of diseased tissue may be negative and histopathologic examination is essential for early diagnosis. Mucormycosis was long regarded as a fatal infection with poor prognosis. However with early medical and surgical management survival rates are now thought to exceed 80%. In the present case, the fungus was identified by hematoxylin and eosin stain and confirmed by Grocott’s silver methenamine special staining technique. Removal of the necrotic bone, which acted as a nidus of infection, was done. Post-operatively patient was advised an obturator to prevent oronasal regurgitation. Since mucormycosis occurs infrequently, it may pose a diagnostic and therapeutic dilemma for those who are not familiar with its clinical presentation.

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STUDIES ON INFLUENZA INFECTION IN FERRETS BY MEANS OF FLUORESCEIN-LABELLED ANTIBODY

Journal of Experimental Medicine ◽

10.1084/jem.101.6.665 ◽

1955 ◽

Vol 101 (6) ◽

pp. 665-676 ◽

Cited By ~ 36

Author(s):

Ch'ien Liu

Keyword(s):

Epithelial Cells ◽

Influenza Infection ◽

Bronchial Epithelium ◽

Mediastinal Lymph Nodes ◽

Viral Antigens ◽

Antibody Staining ◽

Hematoxylin And Eosin ◽

Hematoxylin And Eosin Staining ◽

Ciliated Epithelial Cells ◽

Infectivity Titer

By means of fluorescein-labelled antibody staining, specific influenza viral antigens were seen in both the cytoplasm and the nucleus of infected ciliated epithelial cells covering the nasal turbinates of infected ferrets. Initially, only a small portion of the nasal epithelium showed fluorescence, and no appreciable abnormality of the cells could be detected by hematoxylin and eosin staining. The fluorescence soon spread to involve the entire epithelium, followed by desquamation coinciding with the onset of manifest illness. Pneumonia was seen in some of the infected ferrets, and in them, viral antigens were found in the bronchial epithelium and in the mediastinal lymph nodes. A rise of viral infectivity titer paralleled the observed spread of viral antigens. Many desquamated nasal epithelial cells and macrophages containing antigen were present in nasal smears. The finding would seem to offer a method for the rapid specific diagnosis of influenza infection.

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