The Effect of Irradiated Riboflavin in Human Tenon’s Fibroblast – A Study on Cellular Viability

2021 ◽  
pp. 1-6
Author(s):  
Wendy Yen Nee See ◽  
Fazliana Ismail ◽  
Siti Hamimah Sheikh Abdul Kadir ◽  
Visvaraja Subrayan
Keyword(s):  
Cellular Viability

Demonstration of the cellular viability and safety of Enterococcus faecium CRL 183 in experiments of long term

2007 ◽  
Vol 87 (2) ◽  
pp. 181 ◽  
Author(s):  
Katia Sivieri ◽  
Veridiana P.S. Cano ◽  
Sandro R. Valentini ◽  
Elizeu A. Rossi
Keyword(s):  
Enterococcus Faecium ◽  
Cellular Viability

Artepillin C Induces Selective Oxidative Stress and Inhibits Migration and Invasion in a Comprehensive Panel of Human Cervical Cancer Cell Lines

2019 ◽  
Vol 18 (12) ◽  
pp. 1750-1760 ◽  
Author(s):  
Raquel P. Souza ◽  
Patrícia S. Bonfim-Mendonça ◽  
Gabrielle M.Z.F. Damke ◽  
Analine R.B. de-Assis Carvalho ◽  
Bianca A. Ratti ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cervical Cancer ◽  
Cell Lines ◽  
Migration And Invasion ◽  
Epithelial Cell Line ◽  
Cellular Viability ◽  
Hpv 16 ◽  
Anticancer Properties ◽  
Artepillin C ◽  
Human Cervical Cancer

Background: Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) is the main bioactive component of Brazilian green propolis, and possesses, among other things, anticancer properties. However, to the best of our knowledge, there are no studies of artepillin C in cervical cancer. Method: To explore a new therapeutic candidate for cervical cancer, we have evaluated the effects of artepillin C on cellular viability in a comprehensive panel of human cervical cancer-derived cell lines including HeLa (human papillomavirus/HPV 18-positive), SiHa (HPV 16-positive), CaSki (HPV 16- and 18-positive) and C33A (HPV-negative) cells compared to a spontaneously immortalized human epithelial cell line (HaCaT). Results: Our results demonstrated that artepillin C had a selective effect on cellular viability and could induce apoptosis possibly by intrinsic pathway, likely a result of oxidative stress, in all cancer-derived cell lines but not in HaCaT. Additionally, artepillin C was able to inhibit the migration and invasion of cancer cells. Conclusion: Thus, artepillin C appears to be a promising new candidate as an anticancer drug for cervical cancer induced by different HPV types.

BIO-CORROSION RESISTANCE AND BIOCOMPATIBILITY OF A ZrTi-BASED BMGMC AS POTENTIAL HARD TISSUE IMPLANTS

2013 ◽  
Vol 27 (19) ◽  
pp. 1341029
Author(s):  
XIAOBO HUANG ◽  
JIAOJUAN ZOU ◽  
CHAN WANG ◽  
RUIQIANG HANG ◽  
JUNWEI QIAO ◽  
...  
Keyword(s):  
Cell Culture ◽  
Corrosion Resistance ◽  
Reference Material ◽  
Corrosion Current ◽  
Electrochemical Measurements ◽  
Ti Alloy ◽  
Cellular Viability ◽  
Hard Tissue ◽  
Cellular Behaviors ◽  
Current Densities

In this study, we compared the bio-corrosion resistance and biocompatibility of a ZrTi -based BMGMC ( Zr 58.5 Ti 14.3 Ni 4.9 Cu 6.1 Nb 5.2 Be 11.0). The Ti - 6Al - 4V alloy was used as a reference material. By utilizing the electrochemical measurements and M3T3 cell culture, the corrosion resistance and biocompatibility of this BMGMC were evaluated. The BMGMC displayed high positive corrosion potentials and low corrosion current densities, which indicated that this material exhibited a highly improved corrosion resistance than the Ti alloy. The cells could adhere on the surface of this BMGMC and exhibited improved cellular behaviors, such as cellular viability and cytoskeketal structure. In summary, the ZrTi -based BMGMC showed great potential for applications in the hard tissue implants.

Expression of mutated cationic trypsinogen reduces cellular viability in AR4-2J cells

2005 ◽  
Vol 334 (2) ◽  
pp. 721-728 ◽  
Author(s):  
Sebastian Gaiser ◽  
Astrid Ahler ◽  
Felix Gundling ◽  
Marie-Luise Kruse ◽  
Vuk Savkovic ◽  
...  
Keyword(s):  
Cellular Viability ◽  
Cationic Trypsinogen

scholarly journals Comparative studies of cellular viability levels on 2D and 3D in vitro culture matrices

2017 ◽  
Vol 70 (1) ◽  
pp. 261-273 ◽  
Author(s):  
M. Gargotti ◽  
U. Lopez-Gonzalez ◽  
H. J. Byrne ◽  
A. Casey
Keyword(s):  
In Vitro Culture ◽  
Comparative Studies ◽  
Cellular Viability ◽  
2D And 3D

scholarly journals Ruthenacarborane and Quinoline: A Promising Combination for the Treatment of Brain Tumors

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3801
Author(s):  
Dijana Drača ◽  
Milan Marković ◽  
Marta Gozzi ◽  
Sanja Mijatović ◽  
Danijela Maksimović-Ivanić ◽  
...  
Keyword(s):  
Brain Tumors ◽  
Glucose Deprivation ◽  
Resistance Mechanisms ◽  
Mechanisms Of Action ◽  
Molar Ratio ◽  
Cellular Viability ◽  
Human Glioma ◽  
Acid Complex ◽  
Attractive Candidate ◽  
U251 Cells

Gliomas and glioblastomas are very aggressive forms of brain tumors, prone to the development of a multitude of resistance mechanisms to therapeutic treatments, including cytoprotective autophagy. In this work, we investigated the role and mechanism of action of the combination of a ruthenacarborane derivative with 8-hydroxyquinoline (8-HQ), linked via an ester bond (complex 2), in rat astrocytoma C6 and human glioma U251 cells, in comparison with the two compounds alone, i.e., the free carboxylic acid (complex 1) and 8-HQ, and their non-covalent combination ([1 + 8-HQ], in 1:1 molar ratio). We found that only complex 2 was able to significantly affect cellular viability in glioma U251 cells (IC50 11.4 μM) via inhibition of the autophagic machinery, most likely acting at the early stages of the autophagic cascade. Contrary to 8-HQ alone, complex 2 was also able to impair cellular viability under conditions of glucose deprivation. We thus suggest different mechanisms of action of ruthenacarborane complex 2 than purely organic quinoline-based drugs, making complex 2 a very attractive candidate for evading the known resistances of brain tumors to chloroquine-based therapies.

Finite Element Study of Oxygen Diffusion in the Intervertebral Disc

2000 ◽  
Author(s):  
E. Sélard ◽  
A. Shirazi-Adl ◽  
J. P. G. Urban
Keyword(s):  
Intervertebral Disc ◽  
Blood Supply ◽  
Oxygen Diffusion ◽  
Consumption Rate ◽  
The Body ◽  
Cellular Viability ◽  
Two Dimensional ◽  
Concentration Gradients ◽  
Exchange Area ◽  
The Matrix

Abstract The intervertebral disc consists of a water-rich extra-cellular matrix which is synthesized and maintained by its cells. The disc is the largest avascular tissue in the body with its cells lying as much as 8mm away from the blood supply. Nutrients, essential for maintaining cellular viability, diffuse through the matrix from blood supply under a concentration gradient arising from cellular demand. The oxygen concentration gradients in the intervertebral disc are investigated to examine the effects of exchange area and disc thickness on oxygen flux in the disc. The concentration gradients are computed using the two-dimensional Poisson’s equation and measured values for oxygen consumption rate and oxygen diffusion.

scholarly journals Effects of a New Bioceramic Material on Human Apical Papilla Cells

2018 ◽  
Vol 9 (4) ◽  
pp. 74 ◽  
Author(s):  
Diana Sequeira ◽  
Catarina Seabra ◽  
Paulo Palma ◽  
Ana Cardoso ◽  
João Peça ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mineral Trioxide Aggregate ◽  
Cellular Adhesion ◽  
Cellular Migration ◽  
Cellular Viability ◽  
Migration Rates ◽  
Apical Papilla ◽  
And Migration ◽  
Proliferation And Migration

Background: The development of materials with bioregenerative properties is critically important for vital pulp therapies and regenerative endodontic procedures. The aim of this study was to evaluate the cytocompatibility and cytotoxicity of a new endodontic biomaterial, PulpGuard, in comparison with two other biomaterials widely used in endodontic procedures, ProRoot Mineral Trioxide Aggregate (MTA) and Biodentine. Methods: Apical papilla cells (APCs) were isolated from third molars with incomplete rhizogenesis from patients with orthodontic indication for dental extraction. Cultured APCs were incubated for 24, 48, or 72 h with different dilutions of eluates prepared from the three materials. Cellular viability, mobility, and proliferation were assessed in vitro using the Alamar Blue assay and a wound-healing test. The cells were also cultured in direct contact with the surface of each material. These were then analyzed via Scanning Electron Microscopy (SEM), and the surface chemical composition was determined by Energy-Dispersive Spectroscopy (EDS). Results: Cells incubated in the presence of eluates extracted from ProRoot MTA and PulpGuard presented rates of viability comparable to those of control cells; in contrast, undiluted Biodentine eluates induced a significant reduction of cellular viability. The wound-healing assay revealed that eluates from ProRoot MTA and PulpGuard allowed for unhindered cellular migration and proliferation. Cellular adhesion was observed on the surface of all materials tested. Consistent with their disclosed composition, EDS analysis found high relative abundance of calcium in Biodentine and ProRoot MTA and high abundance of silicon in PulpGuard. Significant amounts of zinc and calcium were also present in PulpGuard discs. Concerning solubility, Biodentine and ProRoot MTA presented mild weight loss after eluate extraction, while PulpGuard discs showed significant water uptake. Conclusions: PulpGuard displayed a good in vitro cytocompatibility profile and did not significantly affect the proliferation and migration rates of APCs. Cells cultured in the presence of PulpGuard eluates displayed a similar profile to those cultured with eluates from the widely used endodontic cement ProRoot MTA.

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