Negative impacts of in-vitro oxidative stress on the quality of heat-induced myofibrillar protein gelation during refrigeration

Boron (B) and Calcium (Ca) unbalance in plants during early stages can generate oxidative stress and consequently to interfere negatively on growth and quality of seedlings. This study aims to evaluate the gas exchange and measure the biochemical responses, responding how high concentrations of B and Ca can affect the growth and quality of young Schizolobium parahyba plants. The experimental design used was completely randomised with four treatments [1 - 25 µM B + 5 mM Ca (control); 2 - 25 µM B + 50 mM Ca (Ca high); 3- 250 µM B + 5 mM Ca (B high) and 4 - 250 µM B + 50 mM Ca (B and Ca high)]. Negative impacts on gas exchange, photosynthetic pigments and total glutathione were obtained, besides increases in hydrogen peroxide and electrolyte leakage were verified in plants treated with B and Ca high, indicating oxidative stress. Thus, application 250 µM B combined with 50 mM Ca promoted disorders in plant metabolism, decreasing the growth and quality of young Schizolobium parahyba plants.

Download Full-text

Study assessing the efficacy of herbal teas on bone health and quality of life in a population with osteopenia: rooibos actions on melatonin and tulsi actions on quality of life.

Melatonin Research ◽

10.32794/mr11250095 ◽

2021 ◽

Vol 4 (2) ◽

pp. 270-298

Author(s):

Fahima Munmun ◽

Alyssa Linden ◽

Hunter Hanlon ◽

Hannah Enderby ◽

Paula Witt-Enderby

Keyword(s):

Quality Of Life ◽

Oxidative Stress ◽

Bone Health ◽

Human Mesenchymal Stem Cell ◽

Melatonin Receptors ◽

Cooperative Effort ◽

Translational Study ◽

Before And After

The purpose of the OsTea translational study was to assess the efficacy of teas (tulsi, rooibos, oolong) compared to placebo (coriander) on markers of bone health and quality of life (QOL) in those with osteopenia and on human mesenchymal stem cell (hMSC) differentiation into osteoblasts to identify potential mechanisms of action. Following consumption of tea (3 times/day; 90 days), participants collected a urine sample during the night (10pm-6am) and filled in questionnaires before and after the study. Rooibos consumption demonstrated a significant decrease in urinary CTX levels vs placebo; trended towards increases in nocturnal melatonin levels (p=0.06); significantly decreased serotonin-producing microbes in the gut; and demonstrated trends towards improvements (p=0.09) in QUALIOST emotional parameters. Tulsi consumption primarily affected subjective measures, such as significantly improved scores for PSS, STAI-trait anxiety, and osteoporosis/osteopenia-related parameters in the QUALIOST. To further identify potential mechanisms underlying these actions of rooibos on CTX and melatonin (urinary and gut), rooibos and melatonin effects on human osteoblastogenesis were carried out for 21 days under oxidative stress conditions to mimic osteopenia. Although both rooibos and melatonin protected against oxidative stress-induced loss of osteoblasts in vitro, their underlying mechanisms were different. Melatonin, like tulsi and oolong, demonstrated the greatest protection against oxidative stress at days 10-11 of exposure, which was due to effects on hMSC viability and through melatonin receptors. Rooibos, on the other hand, demonstrated protection at days 10-11 and 20-21, which was through signaling mechanisms involved in differentiation processes and not on cell viability. These findings suggest that the clinical actions of rooibos on decreasing CTX levels in a population with osteopenia may be through a cooperative effort between melatonin and rooibos by protecting hMSC viability against oxidative stress-induced loss and by promoting osteoblast differentiation, respectively. This study also supports the use of tulsi for improving quality of life in a population susceptible to osteoporosis.

Download Full-text

The protective effect of melatonin on the in vitro development of yak embryos against hydrogen peroxide-induced oxidative injury

Zygote ◽

10.1017/s0967199418000412 ◽

2019 ◽

Vol 27 (3) ◽

pp. 118-125 ◽

Cited By ~ 1

Author(s):

Wei Peng ◽

Mengtong Lei ◽

Jun Zhang ◽

Yong Zhang

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Critical Role ◽

Underlying Mechanism ◽

Preimplantation Embryos ◽

Effective Antioxidant ◽

Vitro Development ◽

Oxidation Effect

SummaryMelatonin plays a critical role in several types of cells as an antioxidant to protect intracellular molecules from oxidative stress. The anti-oxidation effect of melatonin in yak embryos is largely unknown. We report that melatonin can protect the development of yak preimplantation embryos against oxidative stress induced by hydrogen peroxide (H2O2). Therefore, the quality of blastocysts developed from zygotes exposed to H2O2 was promoted. In addition, we observed that melatonin reduced H2O2-induced intracellular reactive oxygen species (ROS) levels and prevented mitochondrial dysfunction in zygotes. These phenomena revealed the effective antioxidant activity of melatonin to prevent oxidative stress in yak embryos. To determine the underlying mechanism, we further demonstrated that melatonin protected preimplantation embryos from oxidative damage by preserving antioxidative enzymes. Collectively, these results confirmed the anti-oxidation effect of melatonin in yak embryos that significantly improved the quantity and quality of blastocysts in the in vitro production of embryos in yaks.

Download Full-text

Effect of oxidative stress and apoptosis in granulosa cells on the quality of oocytes and the outcome of in vitro fertilization and embryo transfer (IVF-ET)

Fertility and Sterility ◽

10.1016/j.fertnstert.2008.07.1625 ◽

2008 ◽

Vol 90 ◽

pp. S336-S337 ◽

Cited By ~ 1

Author(s):

Y.-P. Li ◽

J. Liu ◽

J.-M. Zhang ◽

N.-H. Liu ◽

D.-E. Liu ◽

...

Keyword(s):

Oxidative Stress ◽

In Vitro Fertilization ◽

Embryo Transfer ◽

Granulosa Cells ◽

Vitro Fertilization ◽

Ivf Et

Download Full-text

Applications of Melatonin in Female Reproduction in the Context of Oxidative Stress

Oxidative Medicine and Cellular Longevity ◽

10.1155/2021/6668365 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Yonghui Jiang ◽

Huangcong Shi ◽

Yue Liu ◽

Shigang Zhao ◽

Han Zhao

Keyword(s):

Oxidative Stress ◽

Polycystic Ovary ◽

Oocyte Quality ◽

Early Embryo ◽

Female Reproduction ◽

Adverse Conditions ◽

Wide Range

Oxidative stress has been recognized as one of the causal mediators of female infertility by affecting the oocyte quality and early embryo development. Improving oxidative stress is essential for reproductive health. Melatonin, a self-secreted antioxidant, has a wide range of effects by improving mitochondrial function and reducing the damage of reactive oxygen species (ROS). This minireview illustrates the applications of melatonin in reproduction from four aspects: physiological ovarian aging, vitrification freezing, in vitro maturation (IVM), and oxidative stress homeostasis imbalance associated with polycystic ovary syndrome (PCOS), emphasising the role of melatonin in improving the quality of oocytes in assisted reproduction and other adverse conditions.

Download Full-text

Cypermethrin-Induced in vitro Alterations on Oxidative Stress and Quality of Salmo coruhensis Spermatozoa

Indian Journal of Pharmaceutical Education and Research ◽

10.5530/ijper.52.4s.78 ◽

2018 ◽

Vol 52 (4s) ◽

pp. s71-s76 ◽

Cited By ~ 2

Author(s):

Filiz Kutluyer ◽

Mehmet Kocabas ◽

Mine Erisir ◽

Fulya Benzer

Keyword(s):

Oxidative Stress

Download Full-text

Dual role (promotion and inhibition) of transglutaminase in mediating myoﬁbrillar protein gelation under malondialdehyde-induced oxidative stress

Food Chemistry ◽

10.1016/j.foodchem.2021.129453 ◽

2021 ◽

Vol 353 ◽

pp. 129453

Author(s):

Yuanqi Lv ◽

Xianchao Feng ◽

Rong Yang ◽

Shan Qian ◽

Yaping Liu ◽

...

Keyword(s):

Oxidative Stress ◽

Myofibrillar Protein ◽

Dual Role ◽

Protein Gelation

Download Full-text

A Study on the Quality and Identity of Brazilian Pampa Biome Honey: Evidences for Its Beneficial Effects against Oxidative Stress and Hyperglycemia

International Journal of Food Science ◽

10.1155/2014/470214 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 11

Author(s):

L. C. Cruz ◽

J. E. S. Batista ◽

A. P. P. Zemolin ◽

M. E. M. Nunes ◽

D. B. Lippert ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Antioxidant Properties ◽

High Concentration ◽

Beneficial Effects ◽

Pampa Biome ◽

First Time

We characterized, for the first time, the quality and identity of Brazilian Pampa biome honey and its antioxidant propertiesin vitro(FRAP, DDPH and ABTS). The potential protective effect of honey against oxidative stress induced by iron (Fe) and paraquat, (PQ) in aDrosophila melanogastermodel (in vivo) was also tested. The results indicated that all honey samples tested showed antioxidant activityin vitro. Flies treated with honey showed increased lifespan and were protected against oxidative stress induced by Fe and PQ. Despite the high concentration of sugars in honey (approximately 70–80%), our results demonstrate a hypoglycemic-like effect of honey inDrosophila. Thus, this study demonstrates the high quality of Brazilian Pampa biome honey as well as its significant antioxidant activityin vitroandin vivo, pointing to the potential use of this natural product as an alternative in the therapy of oxidative stress-associated diseases.

Download Full-text