scholarly journals Calcium Binding by Particle-Free Supernatants of Homogenates of Skeletal Muscle

1965 ◽  
Vol 49 (1) ◽  
pp. 131-149 ◽  
Author(s):  
F. Norman Briggs ◽  
Martin Fleishman
Keyword(s):  
Skeletal Muscle ◽  
Molecular Weight ◽  
Stability Constant ◽  
Calcium Binding ◽  
Weight Fraction ◽  
Preliminary Evidence ◽  
High Molecular Weight Fraction ◽  
Total Calcium ◽  
Chelex 100 ◽  
Binding Substance

A high molecular weight fraction of a soluble Marsh muscle-relaxing preparation has been shown to contain a calcium-complexing substance. By examining the nature of the competition between this fraction and chelex-100 for calcium at various total calcium concentrations it has been possible to calculate the concentration and calcium stability constant of this calcium-complexing substance. Taking into account dilutions which occur during the preparation of fractions containing this substance its concentration may be estimated at about 2·10-4 in muscle and its calcium stability constant was found to be about 1.5·105 M-1. Preliminary evidence suggests that the calcium-binding substance is a protein.

Studies on the Functionality of Newly Synthesized Fibrinogen after Treatment of Acute Myocardial Infarction with Streptokinase, Increase in the Rate of Fibrinopeptide Release

1993 ◽  
Vol 70 (06) ◽  
pp. 0978-0983 ◽  
Author(s):  
Edelmiro Regano ◽  
Virtudes Vila ◽  
Justo Aznar ◽  
Victoria Lacueva ◽  
Vicenta Martinez ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Molecular Weight ◽  
Steady State ◽  
High Molecular Weight ◽  
Coronary Arteries ◽  
Risk Index ◽  
Weight Fraction ◽  
High Molecular Weight Fraction ◽  
Fibrinopeptide A

SummaryIn 15 patients with acute myocardial infarction who received 1,500,000 U of streptokinase, the gradual appearance of newly synthesized fibrinogen and the fibrinopeptide release during the first 35 h after SK treatment were evaluated. At 5 h the fibrinogen circulating in plasma was observed as the high molecular weight fraction (HMW-Fg). The concentration of HMW-Fg increased continuously, and at 20 h reached values higher than those obtained from normal plasma. HMW-Fg represented about 95% of the total fibrinogen during the first 35 h. The degree of phosphorylation of patient fibrinogen increased from 30% before treatment to 65% during the first 5 h, and then slowly declined to 50% at 35 h.The early rates of fibrinopeptide A (FPA) and phosphorylated fibrinopeptide A (FPAp) release are higher in patient fibrinogen than in isolated normal HMW-Fg and normal fibrinogen after thrombin addition. The early rate of fibrinopeptide B (FPB) release is the same for the three fibrinogen groups. However, the late rate of FPB release is higher in patient fibrinogen than in normal HMW-Fg and normal fibrinogen. Therefore, the newly synthesized fibrinogen clots faster than fibrinogen in the normal steady state.In two of the 15 patients who had occluded coronary arteries after SK treatment the HMW-Fg and FPAp levels increased as compared with the 13 patients who had patent coronary arteries.These results provide some support for the idea that an increased synthesis of fibrinogen in circulation may result in a procoagulant tendency. If this is so, the HMW-Fg and FPAp content may serve as a risk index for thrombosis.

In Vivo Studies on the Inhibition of Coagulation by Fractionated Heparin and by a Heparin Analogue I. Effects of Heparin Fractions

1981 ◽  
Vol 46 (03) ◽  
pp. 612-616 ◽  
Author(s):  
U Schmitz-Huebner ◽  
L Balleisen ◽  
F Asbeck ◽  
J van de Loo
Keyword(s):  
Molecular Weight ◽  
Day Treatment ◽  
Gel Filtration ◽  
Weight Fraction ◽  
In Vivo Studies ◽  
Low Molecular Weight ◽  
High Molecular Weight Fraction ◽  
Platelet Factor ◽  
Heparin Fractions

SummaryHigh and low molecular weight heparin fractions obtained by gel filtration chromatography of sodium mucosal heparin were injected subcutaneously into six healthy volunteers and compared with the unfractionated substance in a cross-over trial. Equal doses of 5,000 U were administered twice daily over a period of three days and heparin activity was repeatedly controlled before and 2, 4, 8 hrs after injection by means of the APTT, the anti-Xa clotting test and a chromogenic substrate assay. In addition, the in vivo effect of subcutaneously administered fractionated heparin on platelet function was examined on three of the volunteers. The results show that s.c. injections of the low molecular weight fraction induced markedly higher anti-Xa activity than injections of the other preparations. At the same time, APTT results did not significantly differ. Unfractionated heparin and the high molecular weight fraction enhanced ADP-induced platelet aggregation and collagen-mediated MDA production, while the low molecular weight fraction hardly affected these assays, but potently inhibited thrombin-induced MDA production. All heparin preparations stimulated the release of platelet Factor 4 in plasma. During the three-day treatment periods, no side-effects and no significant changes in the response to heparin injections were detected.

scholarly journals ‘Tanned’ gelatin spheres and granules for exclusion chromatography

1968 ◽  
Vol 108 (4) ◽  
pp. 641-646 ◽  
Author(s):  
A. Polson ◽  
W. Katz
Keyword(s):  
Molecular Weight ◽  
Polyethylene Glycol ◽  
High Molecular Weight ◽  
Weight Fraction ◽  
High Flow ◽  
High Molecular Weight Fraction ◽  
Flow Rates ◽  
Protein Molecules ◽  
Exclusion Chromatography

1. The preparation of tanned gelatin spheres and granules from high-molecular-weight gelatin is described. This material is comparatively hard, giving high flow rates, is insoluble in water at temperatures between 0° and 100° and is resistant to digestion by trypsin and chymotrypsin. The high-molecular-weight fraction of gelatin was prepared by precipitation with polyethylene glycol, and the spheres and granules prepared from this fraction were hardened and insolubilized by tanning with either formalin or chromium salts or both. 2. The spheres and granules were used successfully for the separation of protein molecules and other protein-aceous materials ranging in molecular weight from 200 to greater than 6000000. This gel exclusion material has several properties superior to those of other products used for similar purposes. Further, it was noticed that the porosity of the spheres differed considerably from that of the granules.

Sorption of GR-S Type of Polymer on Carbon Black. III. Sorption by Commercial Blacks

1953 ◽  
Vol 26 (1) ◽  
pp. 102-114 ◽  
Author(s):  
I. M. Kolthoff ◽  
R. G. Gutmacher
Keyword(s):  
Molecular Weight ◽  
Carbon Black ◽  
Intrinsic Viscosity ◽  
Benzene Solution ◽  
Weight Fraction ◽  
High Molecular Weight Fraction ◽  
Molecular Weights ◽  
Bound Rubber ◽  
Flow Through ◽  
Peculiar Behavior

Abstract The sorption capacities toward GR-S five commercial carbon blacks are in decreasing order: Spheron-6, Vulcan-1, Philblack-0, Sterling-105, Philblack-A. Apparently, the sorption is not related to surface area. The sorption on Vulcan-1 of GR-S from its solutions in seven different solvents or mixtures of solvents increases with decreasing solvent power for the rubber. The sorption curves of two “cold rubbers,” polymerized at −10 and +5° respectively, showed little difference from that of 50° GR-S. Previous heating of carbon black in nitrogen at 500 or 1100° increased the sorption by about 20 per cent over unheated carbon. Air-heating of carbon black at 425° did not cause a difference in the sorption from benzene solution, but produced an increase in the sorption of rubber from n-heptane solution. In the range 75% butadiene-25% styrene to 5% butadiene-95% styrene, there is practically no effect of the degree of unsaturation on the sorption. Polystyrene of high intrinsic viscosity exhibits a peculiar behavior with furnace blacks. Vulcan-1 sorbed microgel as well as the sol fraction from n-heptane solutions of GR-S containing microgel (conversion 74.7 and 81.5 per cent). There was no appreciable difference in the amount of sorption of rubber fractions having average molecular weights varying from 433,000 to 85,000. There is little change in the amount sorbed after two hours of shaking, but the intrinsic viscosity of the residual rubber decreases with time. The low molecular-weight rubber is sorbed more rapidly, but is slowly replaced by the more tightly sorbed high molecular weight fraction. Partial fractionation of a rubber sample can be achieved by allowing the rubber solution to flow through a column of weakly sorbing carbon black. A large portion of the sorbed rubber can be recovered from the column by washing it with a good solvent such as xylene. Bound rubber is produced by intimate mixing of equal parts of carbon black and rubber swollen in chloroform, when the mixture is dried in vacuum at 80° or at room temperature. Milling is not essential to get bound rubber.

Some Osmotic Measurements of the Molecular Weight of a High Molecular-Weight Fraction of Rubber Hydrocarbon

1955 ◽  
Vol 28 (2) ◽  
pp. 504-507
Author(s):  
G. W. Drake
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Average Molecular Weight ◽  
Weight Fraction ◽  
Considerable Proportion ◽  
Prolonged Storage ◽  
High Molecular Weight Fraction ◽  
The United Kingdom ◽  
Molecular Weight Fractions ◽  
Temperate Climates

Abstract Fractionation of the rubber hydrocarbon in temperate climates has usually resulted in high molecular-weight fractions, with a molecular weight of the order of one million. Bloomfield has shown that fresh latex contains a considerable proportion of hydrocarbon having an intrinsic viscosity (η) of 10 or over and, therefore, a molecular weight of well over 106. The fractionation technique used by Bloomfield in Malaya has now been applied by the writer to smoked sheet and to F rubber, working in the United Kingdom. No very high molecular-weight fractions were found in the smoked sheet, but the F rubber yielded a fraction of (η)=7.3 and a number average molecular weight 6×106, determined osmometrically. The average molecular weight of natural rubber when freshly prepared is probably well over a million, and includes a substantial portion having a molecular weight of several millions. By the time smoked sheet has reached temperate climates, the high molecular-weight portion has probably been converted to gel. F rubber, presumably because of its different method of preparation, retains the major part of its high molecular-weight material during prolonged storage.

Influence of Commercial Sanitizers on Lipopolysaccharide Production by Salmonella Enteritidis ATCC 13076

2006 ◽  
Vol 69 (12) ◽  
pp. 2889-2895 ◽  
Author(s):  
P. VENTER ◽  
M. ABRAHAM ◽  
J. F. R. LUES ◽  
I. IVANOV
Keyword(s):  
Molecular Weight ◽  
Salmonella Enteritidis ◽  
Weight Fraction ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Individual Compound ◽  
High Molecular Weight Fraction ◽  
Spectrometry Analysis ◽  
Hand Wash ◽  
Cumulative Change

The effect of typical sanitizers on the composition and toxicity of lipopolysaccharides (LPSs) produced by Salmonella Enteritidis ATCC 13076 was analyzed. Salmonella Enteritidis was propagated up to the late exponential phase in the presence of commercial sanitizing solutions. LPS was extracted and derivatized with trifluoroacetylation, and gas chromatography–mass spectrometry analysis and the chromogenic Limulus amoebocyte lysate assay were used to assess the ultrastructure and toxicity of the LPS. The viability and debris formation during growth were evaluated to verify the bactericidal and bacteriostatic effects of the sanitizers and to assess sanitizer effects on LPS formation. The LPSs produced were quantified at 1.7 × 104, 1.2 × 104, 3.6 × 103, and 9.6 × 104 [KDO]·minus;1 for the controls and the organisms grown in the presence of a chlorinated sanitizer, a heavy-duty alkaline cleaner, and a phenolic hand wash solution, respectively. In response to these treatments, the short-chain polysaccharide fractions of the LPSs in the Salmonella Enteritidis cells increased. This finding suggests that this organism increases the low-molecular-weight fraction of the LPS in relation to the high-molecular-weight fraction to survive these unfavorable conditions. The cumulative change in the LPS in response to the sanitizers influenced the toxicity of the LPS; however, this change could not be related to an individual compound within any of the assessed fractions.

Uptake of Zinc by the Aquatic Larvae of Simulium ornatipes (Diptera : Nematocera)

1978 ◽  
Vol 29 (3) ◽  
pp. 299 ◽  
Author(s):  
JGT Carter ◽  
WL Nicholas
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Free Water ◽  
Weight Fraction ◽  
The Body ◽  
Ion Concentration ◽  
High Molecular Weight Fraction ◽  
Phosphonic Acids ◽  
Calcium Ion Concentration ◽  
Molecular Weight Fractions

The uptake and loss of zinc by the aquatic larvae of the blackfly S. ornatipes was investigated using radioactive 65Zn. Larvae may absorb significant quantities of zinc from solution, and a substantial proportion remains in the body when larvae are transferred to zinc-free water. Uptake is assisted by metabolism, but an increase of the calcium ion concentration, although reducing toxicity, has no effect on uptake, exchange or the loss of zinc. Larvae may be fractionated into 'cuticle', 'high-' and 'low-molecular-weight' fractions, based on solubility in water and 80% (v/v) ethanol. In the cuticle and high-molecular-weight fractions two 'pools' may be identified by dialysis against Na3EDTA -a pool in which zinc is weakly held and exchanges rapidly with the zinc in solution, and one where zinc is held and exchanges slowly. Exposure time, temperature, and external concentration influence the quantity of zinc entering these pools. Washing the cuticle and high-molecular-weight fractions with a series of buffers suggests that zinc is bound by phenolic groups in the cuticle fraction, and by phosphonic acids in the high-molecular-weight fraction. Sulfhydryl groups did not bind a major portion of the zinc.

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