Long-term antibacterial efficacy of air plasma-activated water

2011 ◽  
Vol 44 (47) ◽  
pp. 472001 ◽  
Author(s):  
Matthew J Traylor ◽  
Matthew J Pavlovich ◽  
Sharmin Karim ◽  
Pritha Hait ◽  
Yukinori Sakiyama ◽  
...  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shuyi Wu ◽  
Jianmeng Xu ◽  
Leiyan Zou ◽  
Shulu Luo ◽  
Run Yao ◽  
...  

AbstractPeri-implant infection is one of the biggest threats to the success of dental implant. Existing coatings on titanium surfaces exhibit rapid decrease in antibacterial efficacy, which is difficult to promisingly prevent peri-implant infection. Herein, we report an N-halamine polymeric coating on titanium surface that simultaneously has long-lasting renewable antibacterial efficacy with good stability and biocompatibility. Our coating is powerfully biocidal against both main pathogenic bacteria of peri-implant infection and complex bacteria from peri-implantitis patients. More importantly, its antibacterial efficacy can persist for a long term (e.g., 12~16 weeks) in vitro, in animal model, and even in human oral cavity, which generally covers the whole formation process of osseointegrated interface. Furthermore, after consumption, it can regain its antibacterial ability by facile rechlorination, highlighting a valuable concept of renewable antibacterial coating in dental implant. These findings indicate an appealing application prospect for prevention and treatment of peri-implant infection.


e-Polymers ◽  
2019 ◽  
Vol 19 (1) ◽  
pp. 268-276 ◽  
Author(s):  
Wen Xue ◽  
Mengxing Zhang ◽  
Fan Zhao ◽  
Fujun Wang ◽  
Jing Gao ◽  
...  

AbstractWound infection is a significant burden on public health. Most present antibacterial agents are typically toxic and devoid of long-term durability. We reported an antimicrobial microcapsule with Chinese nutgall (CN) encapsulated, which was a plant-derived extraction. It is biocompatible and has been used in traditional medicine systems. Sodium alginate (SA) and chitosan worked as shells. The promise of the design is to adopt biocompatible natural polymers and electrostatic attractive chitosan and SA form stable shells to keep long-term release of CN. The results exhibited microcapsules with integrated performance of biocompatibility, long-term durability (inhibition rate of 98.99% against S. aureus after 12 h and 100% after 12 h, 99.61% against E. coli after 6 h and 100% after 12 h), high antibacterial efficacy (with S. aureus inhibition zones of 7.67 mm and E. coli inhibition zones of 5.27 mm) and ease of storage (-20°C for more than 60 h). Their successful fabrication may provide new insights into application of traditional cotton gauze in a sustainable and multifunctional form.


2019 ◽  
Vol 377 ◽  
pp. 98-105 ◽  
Author(s):  
Yongping Zheng ◽  
Songjie Wu ◽  
Jie Dang ◽  
Shifang Wang ◽  
Zhengxin Liu ◽  
...  

2020 ◽  
Vol 17 (8) ◽  
pp. 1900270 ◽  
Author(s):  
Zimu Xu ◽  
Xiaoxia Zhou ◽  
Weishu Yang ◽  
Yudi Zhang ◽  
Zhengxin Ye ◽  
...  

2020 ◽  
Vol 10 (18) ◽  
pp. 6378
Author(s):  
Se Hoon Ki ◽  
Hyeongjin Noh ◽  
Geum Ran Ahn ◽  
Seong Hwan Kim ◽  
Nagendra K. Kaushik ◽  
...  

Plasma-activated water (PAW) has emerged as a platform for sterilizing fungal pathogens. In this study, we investigated the influence of PAW on black melanized spores of Aspergillus brasiliensis to explore the mechanism of fungal spore inactivation. PAW was prepared by activating deionized water with a nonthermal atmospheric pressure air plasma jet (soft plasma jet). The concentrations of H2O2 and NOx in the PAW treated by the soft plasma jet for 3 min were 50 μM and 1.8 mM, respectively, and the pH of the PAW was 3.10. The reactive oxygen and nitrogen species (RONS) in the PAW increased with longer plasma activation time. After being treated for 30 min in the PAW with a plasma activation time of 3 min, the spore viability dramatically dropped to 15%. The viabilities of 0.3% H2O2- and 0.3% HNO3-treated spores were 22% and 42%, respectively. The breakage of the spore cell wall by the PAW was revealed in scanning electron microscope images and flow cytometry measurements. Disruption of cell wall integrity provides a path for intracellular components to escape and RONS of the PAW can attack intracellular components directly. Degradation of high molecular genomic DNA was also observed by agarose gel electrophoresis. These results suggest that long-lived reactive species generated in the PAW play an important role in the inactivation of melanized fungal spores. Consequently, PAW produced by a soft plasma jet can be applied to sterilize bioprotective walled fungal spores in a relatively large volume.


Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1491
Author(s):  
Chia-Min Lin ◽  
Chun-Ping Hsiao ◽  
Hong-Siou Lin ◽  
Jian Sin Liou ◽  
Chang-Wei Hsieh ◽  
...  

Eggs are one of the most commonly consumed food items. Currently, chlorine washing is the most common method used to sanitize shell eggs. However, chlorine could react with organic matters to form a potential carcinogen, trihalomethanes, which can have a negative impact on human health. Plasma-activated water (PAW) has been demonstrated to inactivate microorganisms effectively without compromising the sensory qualities of shell eggs. For this study, various amounts (250, 500, 750, or 1000 mL) of PAW were generated by using one or two plasma jet(s) at 60 watts for 20 min with an air flow rate at 6 or 10 standard liters per minute (slm). After being inoculated with 7.0 log CFU Salmonella Enteritidis, one shell egg was placed into PAW for 30, 60, or 90 s with 1 or 2 acting plasma jet(s). When 2 plasma jets were used in a large amount of water (1000 mL), populations of S. Enteritidis were reduced from 7.92 log CFU/egg to 2.84 CFU/egg after 60 s of treatment. In addition, concentrations of ozone, hydrogen peroxide, nitrate, and nitrite in the PAW were correlated with the levels of antibacterial efficacy. The highest concentrations of ozone (1.22 ppm) and nitrate (55.5 ppm) were obtained with a larger water amount and lower air flow rate. High oxidation reduction potential (ORP) and low pH values were obtained with longer activation time, more plasma jet, and a lower air flow rate. Electron paramagnetic resonance (EPR) analyses demonstrated that reactive oxygen species (ROS) were generated in the PAW. The observation under the scanning electron microscope (SEM) revealed that bacterial cells were swollen, or even erupted after treatment with PAW. These results indicate that the bacterial cells lost control of cell permeability after the PAW treatment. This study shows that PAW is effective against S. Enteritidis on shell eggs in a large amount of water. Ozone, nitrate, and ROS could be the main causes for the inactivation of bacterial cells.


2011 ◽  
Vol 488-489 ◽  
pp. 9-12
Author(s):  
Dong Liu ◽  
Peter E.J. Flewitt ◽  
Keith R. Hallam

In this paper, we explored the relationship between thermal aging and the residual stresses developed within a thermal barrier coating (TBC) and the underlying thermally grown oxide (TGO). Superalloy model specimens (CMSX4) with a curved geometry designed to simulate key features of turbine blades coated with air plasma sprayed (APS) 7 wt.% Y2O3-stabilised ZrO2 applied to an Amdry 995 bond coat were oxidised in the temperature range 900°C to 975°C for various lengths of time. Stress measurements on both the TBC and the underlying TGO were made at positions of known curvature using Raman and photo-stimulated piezo-luminescence spectroscopy (PLPS). The residual stress values reflect the interaction between TGO and TBC, which has the potential to be used as an indicator of service lifetime. The results are discussed with respect to the origin of the stress changes and the long term integrity of the coating.


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