Research on the inhibition for aseptic loosening of artificial joints by Sr-doped calcium polyphosphate (SCPP) in vivo

Abstract Aseptic loosening following periprosthetic osteolysis is the primary complication that limits the lifetime of total joint arthroplasty (TJA). The wear particles trigger a chronic inflammation response in the periprosthetic tissue and turn over the bone balance to bone resorption. The present study aimed to investigate the possible effect and mechanism of strontium ranelate (SR), a clinically safe drug for osteoporosis, on particle-induced periprosthetic osteolysis. Thirty-six female C57BL/6j mice underwent tibial Ti-nail implantation to establish an animal model of aseptic loosening. After 12 weeks, micro-CT results showed that strontium ranelate could inhibit periprosthetic bone resorption. In vitro, Ti particles were used to stimulate RAW264.7 cell line to collect conditioned medium, and co-culture MC3T3-E1 cell line with conditioned medium to establish a cell model of aseptic loosening. The results of alkaline phosphatase (ALP) detection, immunofluorescence, and flow cytometry demonstrated that strontium ranelate could regulate the expression of OPG/RANKL, promote differentiation and mineralization, and inhibit apoptosis in osteoblasts. Moreover, we revealed that SR’s exerted its therapeutic effect by down-regulating sclerostin, thereby activating the Wnt/β-catenin signal pathway. Therefore, this research suggests that strontium ranelate could be a potential drug for the prevention and treatment of particle-induced aseptic loosening post-TJA.

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Amentoflavone Inhibits Osteoclastogenesis and Wear Debris-Induced Osteolysis via Suppressing NF-κB and MAPKs Signaling Pathways

Planta Medica ◽

10.1055/s-0043-124594 ◽

2018 ◽

Vol 84 (11) ◽

pp. 759-767 ◽

Cited By ~ 2

Author(s):

Zhen Zhang ◽

Shuai Zhao ◽

Xiaolei Li ◽

Xiaoqi Zhuo ◽

Wu Zhang ◽

...

Keyword(s):

Signaling Pathways ◽

Aseptic Loosening ◽

Wear Debris ◽

Mitogen Activated Protein Kinase ◽

Micro Computed Tomography ◽

Computed Tomography Scanning ◽

Mitogen Activated Protein ◽

And Function

AbstractWear debris-induced osteolysis is one of the major reasons for subsequent aseptic loosening after cementless hip arthroplasty. Increasing evidence suggests that receptor activator of nuclear factor kappa-B (NF-κB) ligand-mediated osteoclastogenesis and osteolysis are responsible for wear debris-induced aseptic loosening. In the present study, we explored the effect of amentoflavone (AMF) on inhibiting osteoclast generation and wear debris-induced osteolysis in vitro and in vivo. Twenty-four male C57BL/J6 mice were randomly divided into four groups: a sham group and groups with titanium wear debris treatment followed by intraperitoneal injection of various concentrations of AMF (0, 20, and 40 mg/kg/day). The micro computed tomography scanning and histological analysis were performed. Bone marrow-derived macrophages were cultured to investigate the effect of AMF on osteoclast generation and function. The results showed that AMF suppressed osteoclastogenesis, F-actin ring formation, and bone absorption without cytotoxicity. AMF prevented titanium wear debris-induced osteolysis in mice. AMF suppressed the relative proteins of NF-κB and mitogen-activated protein kinase (MAPKs) signaling pathways. Thus, the present study suggests that AMF derived from plants could inhibit osteoclastogenesis and titanium wear debris-induced osteolysis via suppressing NF-κB and MAPKs signaling pathways.

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Alumina Ceramic Exacerbates the Inflammatory Disease by Activation of Macrophages and T Cells

International Journal of Molecular Sciences ◽

10.3390/ijms21197114 ◽

2020 ◽

Vol 21 (19) ◽

pp. 7114

Author(s):

Seong-Min Lim ◽

Juyoung Hwang ◽

Hae-Bin Park ◽

Chan Ho Park ◽

Jun-O Jin

Keyword(s):

T Cells ◽

Cd8 T Cells ◽

Fine Particles ◽

Inflammatory Diseases ◽

Artificial Joints ◽

Al2o3 Ceramic ◽

Adaptive Immune Cells ◽

Materials Used

(1) Background: Aluminum oxide (Al2O3) ceramic is one of the materials used for artificial joints, and it has been known that their fine particles (FPs) are provided by the wear of the ceramic. Al2O3 FPs have been shown to induce macrophage activation in vitro; however, the inflammatory effect in vivo has not been studied. (2) Methods: We examined the in vivo effect of Al2O3 FPs on the innate and adaptive immune cells in the mice. (3) Results: Al2O3 FPs promoted the activation of spleen macrophages; however, conventional dendritic cells (cDCs), plasmacytoid DCs (pDCs), and natural killer (NK) cells were not activated. In addition, increases in the CD4 and CD8 T cells was induced in the spleens of the mice treated with Al2O3 FPs, which differentiated into interferon-gamma (IFN-γ)-producing helper T1 (Th1) and cytotoxic T1 (Tc1) cells. Finally, the injection of Al2O3 FPs exacerbated dextran sulfate sodium (DSS)-induced inflammation in the colon, mediated by activated and increased number of CD4 and CD8 T cells. (4) Conclusions: These data demonstrate that FPs of Al2O3 ceramic may contribute to the exacerbation of inflammatory diseases in the patients.

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A Wear Evaluation of Artificial Joints Made of Ti Alloys in Vivo and in Vitro

MRS Proceedings ◽

10.1557/proc-110-703 ◽

1987 ◽

Vol 110 ◽

Author(s):

Zhang Xiaozhong ◽

Ouyang Qin

Keyword(s):

Wear Resistance ◽

Trace Element ◽

Artificial Joints ◽

Control Subjects ◽

Ti Alloys ◽

Wear Evaluation ◽

Ti Content ◽

Wear Tests

AbstractThe results of wear tests of various Ti alloys are presented. The N+ implanted alloy, especially after solution treating and aging, shows a better wear resistance. Ti-trace element studies of prosthetic patients' hair or blood show a higher average Ti content after about 10 years of replacement over the control subjects.

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ZBTB20-mediated titanium particle-induced peri-implant osteolysis by promoting macrophage inflammatory responses

Biomaterials Science ◽

10.1039/d0bm00147c ◽

2020 ◽

Vol 8 (11) ◽

pp. 3147-3163

Author(s):

Junxiong Qiu ◽

Peng Peng ◽

Min Xin ◽

Zhenkang Wen ◽

Zhong Chen ◽

...

Keyword(s):

Aseptic Loosening ◽

Inflammatory Responses ◽

Wear Particles ◽

Titanium Particle ◽

Artificial Joints

Aseptic loosening (AL) caused by wear particles released from implant surfaces is one of the main causes for the failure of artificial joints, which is initiated by macrophage inflammatory responses.

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In vivo study of porous strontium-doped calcium polyphosphate scaffolds for bone substitute applications

Journal of Materials Science Materials in Medicine ◽

10.1007/s10856-009-3713-5 ◽

2009 ◽

Vol 20 (7) ◽

pp. 1505-1512 ◽

Cited By ~ 60

Author(s):

Meng Tian ◽

Feng Chen ◽

Wei Song ◽

Yancheng Song ◽

Yuanwei Chen ◽

...

Keyword(s):

Bone Substitute ◽

In Vivo Study ◽

Calcium Polyphosphate

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Ceramic Roles in Aseptic Loosening: In Vivo Macro- and Microscopic Histological Evaluation

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.758.228 ◽

2017 ◽

Vol 758 ◽

pp. 228-233

Author(s):

Stefano D'Adamio ◽

G. Maccauro ◽

Giuseppe Malerba ◽

Corrado Piconi

Keyword(s):

Aseptic Loosening ◽

Mononuclear Cells ◽

Joint Space ◽

Giant Cells ◽

Histological Evaluation ◽

High Wear Resistance ◽

Limiting Factor ◽

Peripheral Organs ◽

Physical Form

Prosthetic loosening in absence of infection (aseptic loosening) is the most common reason for revision of Total Hip Replacements (THR) Alumina and/or zirconia-based ceramic materials generally displayed high wear resistance and are considered suitable for load bearing application. To characterize the possible chronic cellular reactions to ceramic debris is then relevant in view of their long-term implantation. Low density ceramic pellets obtained by alumina and zirconia (Y-TZP) medical-grade precursors, were sintered at intermediate density and implanted in the patellar tendon of 24 NZW adult rabbits (group A). In this way the progressive release into the joint space of grains detached from surface of the pellets by the motion of the joint. Other 24 NZW rabbits (group B) received 5mg of powders injected in the articular capsule. Animals were killed at different intervals (1,3,6,12 months). Retrieved knee joints underwent X-Ray, histological and ultrastructural analysis. Peripheral organs (liver, kidney, lung, spleen, brain) were collected at same time. For, histologic analysis of tissue specimens collected in revision surgeries were fixed in 10% formalin, then decalcified ethylendiamintetracetic acid (EDTA), dehydrated and embedded in paraffin, sectioned and stained with hematoxylin-eosin. Histologic reaction was similar for the two ceramics and independent of the physical form (powders or pellets). Ceramic particulate was observed in the periprosthetic tissue membrane, the smaller inside mononuclear histiocyte-like cells in granuloma-like tissue, while the largest were in a synovial-like membrane inside a stroma poor in vessel containing mononuclear cells and rarely giant cells. No toxic nor carcinogenic effects were observed in peripheral organs retrieved at different time intervals. Although the ankyloses of the joints is a limiting factor for the duration of the test, this method may be a useful tool to evaluate the reactions to wear debris, and may result suitable also to evaluate the chronic response to metals or polymers of clinical interest.

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