scholarly journals Minimum inhibitory concentration of leaf and fruit extract Ficus lyrata Warb against Salmonella thypi bacteria

2020 ◽  
Vol 443 ◽  
pp. 012046
Author(s):  
D W Wira ◽  
E Mardawati ◽  
R O Hutauruk ◽  
D E M Bangun ◽  
H E Kamila
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Fruit Extract ◽  
Ficus Lyrata

scholarly journals Uji konsentrasi hambat minimum (KHM) ekstrak buah mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Streptococcus mutans

e-GIGI ◽  
2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Jecklin Makolit ◽  
Olivia A. Waworuntu ◽  
Michael A. Leman
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Inhibitory Concentration ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Dilution Method ◽  
Fruit Extract ◽  
Control Group Design ◽  
Group Design ◽  
Serial Dilution Method

Abstract: Noni fruit (Morinda cittrifolia L.) is an herbal plant that contains antibacterial substances, inter alia acubin, lasperuloside, alizarin, and some antraquinons, that have been proved to inhibit the growth of Streptococcus mutans. This study was aimed to obtain the minimum inhibitory concentration (MIC) of noni fruit extract (Morinda citrifolia L.) against the growth of Streptococcus mutans. This was a true experimental study with a randomized pretest-posttest control group design. This study used a serial dilution method with turbidimetry and spectrophotometry tests. Noni fruit was extracted by using maceration method with 96% ethanol. Streptococcus mutans bacteria were obtained from pure stock of Microbiology Laboratory of Pharmacy Program Study, University of Sam Ratulangi Manado. The results showed that the MIC of noni fruit extract against the growth of Streptococcus mutans was at 50% of concentration. Conclusion: The minimum inhibitory concentration of Morinda citrifolia L. extract against the growth of Streptococcus mutans was 50%.Keywords: noni fruit (Morinda citrifolia L.), Streptococcus mutans, MIC Abstrak: Buah mengkudu (Morinda citrifolia, L) merupakan tanaman herbal dengan kandungan bahan berkhasiat antibakterial seperti acubin, lasperuloside, alizarin, dan beberapa zat antraquinon yang terbukti dapat menghambat pertumbuhan Streptococcus mutans. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimum (KHM) dari ekstrak buah mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Streptococcus mutans. Jenis penelitian ialah eksperimental murni dengan randomized pretest-posttest control group design. Metode yang digunakan yaitu metode serial dilusi dengan metode pengujian turbidimetri dan spektrofotometri. Buah mengkudu diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Bakteri Streptococcus mutans diambil dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi. Hasil penelitian menunjukkan bahwa KHM ekstrak buah mengkudu terhadap pertumbuhan Streptococcus mutans pada konsentrasi 50%. Simpulan: Konsentrasi hambat minimum ekstrak buah mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Streptococcus mutans pada konsentrasi 50%.Kata kunci: buah mengkudu (Morinda citrifolia L.), Streptococcus mutans, KHM

scholarly journals Daya Antibakteri Ekstrak Buah Adas (Foeniculum vulgare) Terhadap Bakteri Micrococcus luteus Secara In Vitro [Antibacterial Effort Of Adas Fruit (Foeniculum vulgare) Extract On Micrococcus luteus Bacterial By In Vitro]

2019 ◽  
Vol 2 (1) ◽  
pp. 31
Author(s):  
Rahayu Kusdarwati, Ludira Sari, Akhmad Taufiq Mukti
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Micrococcus Luteus ◽  
Foeniculum Vulgare ◽  
Fruit Extract ◽  
Randomized Design ◽  
Disease Attack ◽  
Development Center

AbstractOne of the disease attack of freshwater fish is micrococcus which caused by Micrococcus luteus bacterial. Antibiotic was caused of residual, polluted and expensive. Therefore, it was looked for alternative using natural and safety materials. The study used the fruit extract of adas as antibacterial to Micrococcus luteus. This experiment purpose to investigate of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of fruit extract of adas to Micrococcus luteus. The study was done on 15 June-05 July 2009 on Development Center of Quarantine Fish Juanda; Institute Tropical Disease, Airlangga University Surabaya; and Department of Faculty Pharmacology of Medicine, Airlangga University Surabaya. The experiment was used experimental method which completely randomized design with twelve treatments and three replicates. The parameters observed were Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The data analyzed used F test and was continued with Duncan's Multiple Range Test. The result indicated that fruit extract of adas had antibactericidal to Micrococcus luteus. According to  inhibitory test growth of bacterial had inhibitory capacity (MIC) at concentration 1,56% (0,018 g/ml). Therefore, the bactericidal capacity (MBC) was at concentration 3,13% (0,036 g/ml). 

scholarly journals DAYA HAMBAT EKSTRAK BUAH ANDALIMAN (Zanthoxylum acanthopodium DC) DALAM ETIL ASETAT TERHADAP PERTUMBUHAN Escherichia coli

2019 ◽  
Vol 8 (3) ◽  
pp. 257
Author(s):  
Fitri Maria Clarensia Sitanggang ◽  
Agus Selamet Duniaji ◽  
I Desak Putu Kartika Pratiwi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Ethyl Acetate ◽  
Experimental Method ◽  
Inhibitory Concentration ◽  
Strong Inhibition ◽  
Clear Zone ◽  
Fruit Extract ◽  
Escherichia Coli Bacteria

This study aims to determine the Minimum Inhibitory Consentration (MIC) of andaliman fruit extract (Zanthoxylum acanthopodium DC) in ethyl acetate against the growth of Escherichia coli bacteria. The design of this study used an experimental method with 10 extract concentrations for MIC testing, namely 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% with 2 repetitions so that they were obtained 20 experimental units. The variables observed were the formation of clear zones, namely the inhibition produced by the presence of antimicrobial activity from andaliman fruit extract in ethyl acetate against the growth of Escherichia coli. Data from research results are presented in the form of tables, figure and discussed descriptively. Andaliman fruit extracts in ethyl acetate have varying inhibitory forces, namely at the concentrations of concentrations of 10% to 20% was 0.65 mm to 3.15 mm with weak inhibition categories, concentrations of 30% to 50% was 5.25 mm to 6, 60 mm in the moderate inhibition category and concentrations of 60% to 100% showed strong inhibition categories with inhibition diameters of 7.20 mm to 9.60 mm. Minimum Inhibitory Concentration (MIC) for the growth of Escherichia coli was at the concentration of 60% with a clear zone diameter of 7.2 mm with a strong category.     Keywords : Andaliman fruit, Escherichia coli, antibacterial.

scholarly journals In Vitro Test: Antimicrobial Activity Potential From Ciplukan Fruit (Physalis minima L.) Extract in Methicillin-resistant Staphylococcus aureus (MRSA)

2020 ◽  
Vol 4 (2) ◽  
pp. 209
Author(s):  
Muhammad Hanun Mahyuddin ◽  
Arifa Mustika ◽  
Gwenny Ichsan Prabowo ◽  
Deby Kusumaningrum
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Test Tube ◽  
In Vitro Test ◽  
Fruit Extract ◽  
Methicillin Resistant

Abstract  In Indonesia, in 2006, the prevalence of infections due to MRSA was 23.5%. Physalis minima L. plants are known to have antimicrobial activity because they contain compounds withaferin A, which can induce programmed cell death. This research was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Ciplukan (Physalis minima L.) extract in Methicillin-resistant Staphylococcus aureus (MRSA) bacteria. Dilution test with Mueller-Hinton broth medium used for measuring the minimum inhibitory concentration (MIC). Ciplukan  fruit extract was dissolved in distilled water, and poured into a test tube with a certain concentration (0.9 g/mL (90%); 0.3 g/mL (45%); 0.15 g/mL (22.5%); 0.075 g/mL (11.25%) and 0.0375 g/mL (5.625%). After being incubated for 24 hours, the bacteria in the test tube were plated on nutrient agar plates to determine the MBC. The MIC cannot be determined, because the medium in the dilution test tube is disturbed by the color of the extract so that turbidity cannot be observed. From the observations of the minimum bactericidal concentration, MBC of the Ciplukan (Physalis minima  L.) fruit extract against MRSA was in the P1 tube or equivalent to 0.9 g / ml (90%).Keywords             : Physalis minima L., Antimicrobial, Ciplukan, Methicillin-resistant Staphylococcus aureus, Withaferin ACorrespondence   : [email protected]

Green and Simple Synthesis of Silver Nanoparticles by Aqueous Extract of Perovskia abrotanoides: Characterization, Optimization and Antimicrobial Activity

2020 ◽  
Vol 21 (11) ◽  
pp. 1129-1137 ◽  
Author(s):  
Somayeh Mirsadeghi ◽  
Masoumeh F. Koudehi ◽  
Hamid R. Rajabi ◽  
Seied M. Pourmortazavi
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Minimum Inhibitory Concentration ◽  
Plant Extract ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Extraction Temperature ◽  
Silver Particles ◽  
Silver Nps ◽  
Perovskia Abrotanoides

Background: Herein, we report the biosynthesis procedure to prepare silver nanoparticles as reduction and capping agents with the aqueous plant extract of Perovskia abrotanoides. Methods: The therapeutic application of silver nanoparticles entirely depends on the size and shape of the nanoparticles therefore, their control during the synthesis procedure is so important. The effects of synthesis factors, for example, silver ion concentration, the mass of plant extract, reaction time and extraction temperature, on the size of silver particles were considered and optimized. Several analytical methods were used for the characterization of silver NPs including FT-IR and UV–Vis spectrophotometer, XRD and SEM. Results: The results showed that the mean size of the silver particles was about 51 nm. Moreover, the antibacterial properties of biosynthesized silver NPs were investigated by the minimum inhibitory concentration, minimum bactericidal concentration, and Well-diffusion tests. The minimum inhibitory concentration/ minimum bactericidal concentration values of silver NPs and aqueous plant extract versus Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and Gram-negative bacteria (E. coli) were 3.03/0.00, 1.20/0.01, 3.06/0.00, 0.98/1.04, 1.00/0.05 and 1.30/0.03 (mg/mL), respectively. Conclusion: The antimicrobial activity study displayed that the synthesized silver nanoparticles by plant extract have better antimicrobial properties compared to aqueous plant extract of Perovskia abrotanoides.

In Vitro Antibacterial activity of ethanolic extract of Myrsine africana against laboratory Strains of Pathogenic Organisms

2016 ◽  
Vol 5 (04) ◽  
pp. 4512
Author(s):  
Jackie K. Obey ◽  
Anthoney Swamy T* ◽  
Lasiti Timothy ◽  
Makani Rachel
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
E Coli ◽  
Zones Of Inhibition ◽  
In Vitro Antibacterial Activity ◽  
Myrsine Africana

The determination of the antibacterial activity (zone of inhibition) and minimum inhibitory concentration of medicinal plants a crucial step in drug development. In this study, the antibacterial activity and minimum inhibitory concentration of the ethanol extract of Myrsine africana were determined for Escherichia coli, Bacillus cereus, Staphylococcus epidermidis and Streptococcus pneumoniae. The zones of inhibition (mm±S.E) of 500mg/ml of M. africana ethanol extract were 22.00± 0.00 for E. coli,20.33 ±0.33 for B. cereus,25.00± 0.00 for S. epidermidis and 18. 17±0.17 for S. pneumoniae. The minimum inhibitory concentration(MIC) is the minimum dose required to inhibit growth a microorganism. Upon further double dilution of the 500mg/ml of M. africana extract, MIC was obtained for each organism. The MIC for E. coli, B. cereus, S. epidermidis and S. pneumoniae were 7.81mg/ml, 7.81mg/ml, 15.63mg/ml and 15.63mg/ml respectively. Crude extracts are considered active when they inhibit microorganisms with zones of inhibition of 8mm and above. Therefore, this study has shown that the ethanol extract of M. africana can control the growth of the four organisms tested.

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