Justification of the need to protect wheat crops against disease agents in the North–Eastern part of the Central Chernozem region

Abstract To develop and optimize protective measures in the CCR conditions, an analysis of the epidemic resistance of zoned varieties and hybrid lines of spring wheat to the causative agents of septoria, brown and yellow rust was carried out; the effect of fungicides, biological agents, their binary mixtures was studied for wheat crops. Among the selection material of spring wheat, lines and numbers were identified and selected as potential genetic sources and donors, combining resistance to environmental stress factors (brown and yellow rust, septoria blight) with productivity and adaptability to the conditions of the Central Black Earth Region. Using DNA markers, Lr genes in the lines selected as potential donors of leaf rust resistance were identified. In the genotypes of selection lines, the Lr19 gene dominance was revealed in combination with the weakly effective genes Lr10, Lr 20, and Lr 26. It was observed that the combination of Lr19 + Lr26 increases the level of resistance. All zoned varieties were affected by diseases, which indicates the need for protective measures. The biological effectiveness of chemical and biological agents against aerogenic diseases was established in the range of 82.1-100%. Amistar Extra was effective against septoria, perenophorosis and leaf rust, Rex Duo was effective against leaf rust, Title Duo was effective against powdery mildew. Amist Extra 0.7 l / ha + Biostim 2.0 l / ha was effective against leaf rust (99.4%) and powdery mildew (98.9%). A mixture of Amistar Extra 0.7 l / ha and Alfastim 30 ml / ha was effective against septoria (93.0%) and pyrenoforosis (100%). Mixed forms of Amistar Extra 0.7 l / ha + Alfastim 30 ml / ha (91.3-95.7%) and Amistar Extra 0.7 l / ha + Healthy yield 0.9 l / ha (91.0-95.7%) were effective against septoria spot and brown rust.

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Virulence surveillance of wheat black stem rust fungus

Baghdad Science Journal ◽

10.21123/bsj.11.2.803-812 ◽

2014 ◽

Vol 11 (2) ◽

pp. 803-812

Author(s):

Baghdad Science Journal

Keyword(s):

Leaf Rust ◽

Disease Severity ◽

Stem Rust ◽

Yellow Rust ◽

Rust Fungus ◽

The North ◽

Rust Diseases ◽

Northern Regions ◽

Infection Disease ◽

Wheat Rust

General survey for wheat rust diseases in Iraqi fields was done during the seasons of 2010, 2011 and 2012. The survey covered different fields in southern, middle and northern regions. Results of the first season indicated that most of Iraqi cultivars such as Tmmoze2, IPA 99 and Mexipak showed different types of susceptibility to both yellow and leaf rust infection. Disease severity increased when the conditions were favorable for infections with using susceptible cultivars. The severity of leaf rust was less in the north region comparing with the middle and south regions. Most of the introduced cultivars such as Sham6 and Cimmyto showed susceptible reaction to yellow and leaf rust. Yellow rust was in epiphytotic form at the Iraqi-Syrian-Turkish triangle where the disease severity was 100%. Low disease severity of stem rust was observed on some cultivars (1-5%), except for the cultivar Mexipak which showed 40%S in Najaf. Rusts at season of 2011 were restricted mostly in Baghdad and the yellow rust was dominant. The AUDPC of 15 wheat cultivars showed that Sawa and Sali were highly susceptible to the three types of rusts while Babil113 and Tamoze2 were resistant. No rusts were detected at season 2012.

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Use of synthetic form Triticum miguschovae Zhir in common wheat breeding

PROCEEDINGS OF V INTERNATIONAL SCIENTIFIC CONFERENCE “CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE” ◽

10.33952/2542-0720-2020-5-9-10-56 ◽

2020 ◽

Author(s):

R.O. Davoyan ◽

◽

I.V. Bebyakina ◽

E.R. Davoyan ◽

V.A. Bibishev ◽

...

Keyword(s):

Leaf Rust ◽

Common Wheat ◽

Dna Analysis ◽

Yellow Rust ◽

Leaf Rust Resistance ◽

Wheat Breeding ◽

Winter Wheat Cultivars ◽

The Common ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

T. miguschovae (GGAADD) was used as a “genetic bridge” to transfer valuable traits to the common wheat instead T. militina and Ae. tauschii. Lines with resistance to leaf rust, yellow rust and powdery mildew, as well as with high protein content (17–18 %) were selected. The lines with translocation Т2BL.2BS-2GL, 5BS.5BL-5GL, T6BS.6BL-6GL and substitution of chromosomes 1D(1Dt), 4D(4Dt), 5D(5Dt), 6D(6Dt) were identified. DNA analysis revealed that the lines can carry leaf rust resistance genes that are different from the known Lr39 and Lr50. Introgression lines have been successfully used in breeding. Five common winter wheat cultivars are developed.

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Physiologic Specialization and Genetic Differentiation of Puccinia triticina Causing Leaf Rust of Wheat in the Indian Subcontinent during 2016-2019

Plant Disease ◽

10.1094/pdis-06-20-1382-re ◽

2021 ◽

Author(s):

Subhash Chander Bhardwaj ◽

Subodh Kumar ◽

Om Prakash Gangwar ◽

Pramod Prasad ◽

Prem Lal Kashyap ◽

...

Keyword(s):

Leaf Rust ◽

Indian Subcontinent ◽

Puccinia Triticina ◽

Leaf Rust Resistance ◽

International Communication ◽

Field Resistance ◽

Adult Plant ◽

Wheat Varieties ◽

The North ◽

High Degree

Wheat is the second most cultivated cereal in the world and is equally important in India. Leaf (brown) rust, caused by Puccinia triticina, was most prevalent among the three rusts in all the wheat-growing areas of India, Bhutan, and Nepal during 2016 to 2019. Leaf rust samples from wheat crops in these countries were pathotyped using the wheat differential genotypes and binomial Indian system of nomenclature. To facilitate international communication, each pathotype identified was also tested on the North American differentials. A total of 33 pathotypes were identified from 1,086 samples, including 3 new pathotypes, 61R47 (162-5 = KHTDM) and 93R49 (49 = NHKTN) from India and 93R57 (20-1 = NHKTL) from Nepal. Two pathotypes, 121R60-1 (77-9/52 = MHTKL) and 121R63-1 (77-5 = THTTM), accounted for 79.46% of the population. Virulence on Lr19 was identified in 0.27% of the samples and from Nepal only. The proportion of pathotype 121R60-1 (77-9 = MHTKL) increased during these years to 57.55%. Virulence was not observed to Lr9, Lr24, Lr25, Lr28, Lr32, Lr39, Lr45, and Lr47 in the population of the Indian subcontinent. Eighteen polymorphic simple sequence repeat (SSR) primer pairs tested on the isolates amplified 48 alleles with an average of 2.66 alleles per primer pair. Based on SSR genotyping, these pathotypes could be grouped into two clades with further two subclades each. Many of the Lr genes present in Indian wheat germplasm (Lr1, Lr3a, Lr10, Lr11, Lr14a, Lr15, Lr16, Lr17, Lr20, Lr23, and Lr26) were ineffective to a majority of the pathotypes. Most of these varieties possessed a high degree of leaf rust resistance. The field resistance of wheat varieties could be attributed to the interaction of genes, unknown resistance, or adult plant resistance.

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Tracking of powdery mildew and leaf rust resistance genes in Triticum boeoticum and T. urartu, wild relatives of common wheat

Czech Journal of Genetics and Plant Breeding ◽

10.17221/127/2010-cjgpb ◽

2011 ◽

Vol 47 (No. 2) ◽

pp. 45-57 ◽

Cited By ~ 4

Author(s):

N.A. Hovhannisyan ◽

M.E. Dulloo ◽

A.H. Yesayan ◽

H. Knüpffer ◽

A. Amri

Keyword(s):

Powdery Mildew ◽

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Diploid Species ◽

Leaf Rust Resistance ◽

Gene Marker ◽

Sequence Tagged Sites ◽

A Genome ◽

Triticum Boeoticum

Wild Triticum and Aegilops species are increasingly used in wheat breeding programmes around the world as donors of genes conferring resistance to biotic and abiotic stresses, as well as of genes that contribute to the improvement of grain quality. In the present study, thirty-nine accessions of diploid species with the A genome (Triticum boeoticum and T. urartu) were evaluated for the presence of the genes conferring resistance to powdery mildew (Blumeria graminis) and leaf rust (Puccinia recondita) using both inoculation tests and sequence tagged sites (STS) marker analyses in order to find correspondence between STS markers and resistance as a trait. The most resistant entries were T. boeoticum accessions. All the marked Lr and Pm resistance genes (Pm1, Pm2, Pm3, Lr10, Lr47, Lr25 and Lr28) were identified in the check T. aestivum cultivar Bezostaya 1. The resistance to powdery mildew in the material studied was conferred by the combination of the Pm1 gene with either Pm2 or Pm3. The Pm1 and Pm3 markers appeared to be suitable for tracking these powdery mildew resistance genes, while the Pm2 gene marker cannot be considered as usable in various genetically different wheat accessions. The presence of the genes Lr25, Lr28 and Lr47 seems to be particularly useful for obtaining leaf rust resistance in T. boeoticum and T. urartu species.  

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PCR Markers for Triticum speltoides Leaf Rust Resistance Gene Lr51 and Their Use to Develop Isogenic Hard Red Spring Wheat Lines

Crop Science ◽

10.2135/cropsci2005.0728 ◽

2005 ◽

Vol 45 (2) ◽

pp. 728-734 ◽

Cited By ~ 43

Author(s):

M. Helguera ◽

L. Vanzetti ◽

M. Soria ◽

I. A. Khan ◽

J. Kolmer ◽

...

Keyword(s):

Leaf Rust ◽

Resistance Gene ◽

Spring Wheat ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Rust Resistance Gene ◽

Leaf Rust Resistance Gene ◽

Pcr Markers ◽

Hard Red Spring Wheat ◽

Wheat Lines

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Effectiveness of major resistance genes and identification of new sources for disease resistance in wheat

Acta Agronomica Hungarica ◽

10.1556/aagr.59.2011.3.7 ◽

2011 ◽

Vol 59 (3) ◽

pp. 241-248 ◽

Cited By ~ 2

Author(s):

G. Vida ◽

M. Cséplő ◽

G. Gulyás ◽

I. Karsai ◽

T. Kiss ◽

...

Keyword(s):

Disease Resistance ◽

Powdery Mildew ◽

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Genetic Material ◽

Leaf Rust Resistance ◽

Wheat Varieties ◽

Major Resistance Genes ◽

Greenhouse Tests

Among the factors which determine yield reliability an important role is played by disease resistance. One of the breeding aims in the Martonvásár institute is to develop wheat varieties with resistance to major diseases. The winter wheat varieties bred in Martonvásár are examined in artificially inoculated nurseries and greenhouses for resistance to economically important pathogens. The effectiveness of designated genes for resistance to powdery mildew and leaf rust has been monitored over a period of several decades. None of the designated major resistance genes examined in greenhouse tests is able to provide complete resistance to powdery mildew; however, a number of leaf rust resistance genes provide full protection against pathogen attack (Lr9, Lr19, Lr24, Lr25, Lr28 and Lr35). In the course of marker-assisted selection, efficient resistance genes (Lr9, Lr24, Lr25 and Lr29) have been incorporated into Martonvásár wheat varieties. The presence of Lr1, Lr10, Lr26, Lr34 and Lr37 in the Martonvásár gene pool was identified using molecular markers. New sources carrying alien genetic material have been tested for powdery mildew and leaf rust resistance. Valuable Fusarium head blight resistance sources have been identified in populations of old Hungarian wheat varieties. Species causing leaf spots (Pyrenophora tritici-repentis, Septoria tritici and Stagonospora nodorum) have gradually become more frequent over the last two decades. Tests on the resistance of the host plant were begun in Martonvásár four years ago and regular greenhouse tests on seedlings have also been initiated.

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