scholarly journals Isolation and identification of fenobucarb degrading bacteria from Pangalengan farm land

2021 ◽  
Vol 883 (1) ◽  
pp. 012007
Author(s):  
A Akhdiya ◽  
R A Sanjaya ◽  
Wartono
Keyword(s):  
Soil Microbes ◽  
Agricultural Soils ◽  
Soil Samples ◽  
Haemolytic Activity ◽  
Rrna Gene ◽  
Isolation And Identification ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
Degrading Bacteria

Abstract The long term excessive use of pesticides can lead to their residues accumulation in the soils. Soil microbes were considered to convert the residues into harmless compounds, however the indigenous soil microbes having those beneficial properties are limited. Therefore this study aimed to isolate, select and identify the fenobucarb insecticide-degrading bacteria from agricultural soils. The soil samples were collected from the vegetable fields in Pangalengan, West Java, Indonesia. Isolation of the bacteria was conducted using Nitrate Mineral Salt Agar suplemented by 100 ppm of a fenobucarb. The bacteria isolates were selected based on its hypersensitive response, haemolytic activity, and its ability to degrade fenobucarb. The selected isolates was identified base on sequences of 16S rRNA gene. Twenty nine bacteria were isolated from four soil samples and 23 of the isolates were not potentially phytopathogenic and non haemolytic. The best three isolates that could degrade 94.2%, 94.5% and 95.47% fenobucarb residue are B41, B54 and B83 isolates, respectively. The 16S rDNA Sequence analysis showed that B41 and B83 isolates have 100% similarity to Bacillus thuringiensis MYBT 18426B54, while B54 isolate has 99% similarity to Bacillus luciferensis LMG 18422. These isolates are potential to be developed as a bioremediation agent.

Isolation and Identification of High Efficient Oil-Degrading Bacteria and Formulation of Complex Bacteria

2013 ◽  
Vol 316-317 ◽  
pp. 594-598 ◽  
Author(s):  
Wen Jing Hao ◽  
Jun Mu ◽  
Yuan Li
Keyword(s):  
Bacterial Consortium ◽  
Achromobacter Xylosoxidans ◽  
Isolation And Identification ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
Degrading Bacteria ◽  
High Efficient ◽  
Biochemical Identification ◽  
First Time ◽  
Physiological And Biochemical

In this experiment, 25 oil degrading bacteria strains were isolated from the beach near Dalian Bay. The top 4 high efficient oil-degrading bacteria（SQ、SY 、SW、SC） were selected to be studied. Using Physiological and biochemical identification, electron microscopy, and 16S rDNA sequence analysis, strain SQ was identified as Advenella kashmirensis，SY was identified as Achromobacter xylosoxidans, SW was identified as Acinetobacter venetianus，SC was identified as Alcaligenes xylosoxidans subsp.xylosoxidans. For the first time this experiment provided reference to application and degradation characteristics of Advenella kashmirensis in crude oil treatment. These four marine oil degrading bacteria were selected to form bacterial consortium. The most efficient mixed strains were constructed by using these four strains with proportional combination. The oil removal ratio of the optimal mixed strains increased to 70.33%, nearly 25% higher than the highest degradation rate of single bacterium, showing significant synergism in the bacterial consortium. Advenella kashmirensis enhanced oil degradation obviously, playing a crucial role in the bacterial consortium.

scholarly journals Isolation and Identification of Sodium Fluoroacetate Degrading Bacteria from Caprine Rumen in Brazil

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Expedito K. A. Camboim ◽  
Arthur P. Almeida ◽  
Michelle Z. Tadra-Sfeir ◽  
Felício G. Junior ◽  
Paulo P. Andrade ◽  
...  
Keyword(s):  
Rumen Fluid ◽  
16S Rrna Gene Sequencing ◽  
Positive Control ◽  
Control Measures ◽  
Rrna Gene ◽  
Isolation And Identification ◽  
Poisonous Plants ◽  
Degrading Bacteria ◽  
Rrna Gene Sequencing ◽  
Orbital Shaker

The objective of this paper was to report the isolation of two fluoroacetate degrading bacteria from the rumen of goats. The animals were adult goats, males, crossbred, with rumen fistula, fed with hay, and native pasture. The rumen fluid was obtained through the rumen fistula and immediately was inoculated 100 μL in mineral medium added with 20 mmol L−1sodium fluoroacetate (SF), incubated at 39°C in an orbital shaker.Pseudomonas fluorescens(strain DSM 8341) was used as positive control for fluoroacetate dehalogenase activity. Two isolates were identified by 16S rRNA gene sequencing asPigmentiphaga kullae(ECPB08) andAncylobacter dichloromethanicus(ECPB09). These bacteria degraded sodium fluoroacetate, releasing 20 mmol L−1of fluoride ion after 32 hours of incubation in Brunner medium containing 20 mmol L−1of SF. There are no previous reports of fluoroacetate dehalogenase activity forP. kullaeandA. dichloromethanicus. Control measures to prevent plant intoxication, including use of fences, herbicides, or other methods of eliminating poisonous plants, have been unsuccessful to avoid poisoning by fluoroacetate containing plants in Brazil. In this way,P. kullaeandA. dichloromethanicusmay be used to colonize the rumen of susceptible animals to avoid intoxication by fluoroacetate containing plants.

scholarly journals Influence of long-term mineral fertilization on metal contents and properties of soil samples taken from different locations in Hesse, Germany

SOIL ◽  
2015 ◽  
Vol 1 (1) ◽  
pp. 23-33 ◽  
Author(s):  
S. Czarnecki ◽  
R.-A. Düring
Keyword(s):  
Cation Exchange ◽  
Cation Exchange Capacity ◽  
Agricultural Soils ◽  
Mineral Fertilizer ◽  
Fertilizer Application ◽  
Exchange Capacity ◽  
Soil Samples ◽  
Residual Effects ◽  
Metal Contents

Abstract. Essential and non-essential metals occur in soils as a result of weathering, industrial processes, fertilization, and atmospheric deposition. Badly adapted cultivation of agricultural soils (declining pH value, application of unsuitable fertilizers) can enhance the mobility of metals and thereby increase their concentrations in agricultural products. As the enrichment of metals in soils occurs over long time periods, monitoring of the long-term impact of fertilization is necessary to assess metal accumulation in agricultural soils. The main objective of this study was to test the effects of different mineral fertilizer variations on soil properties (pH, Corg, and cation exchange capacity (CEC)) and pseudo-total and mobile metal contents of soils after 14 years of fertilizer application and to determine residual effects of the fertilization 8 years after cessation of fertilizer treatment. Soil samples were taken from a field experiment which was carried out at four different locations (210, 260, 360, and 620 m above sea level) in Hesse, Germany. During the study, a significant decrease in soil pH and an evident increase in soil carbon content and cation exchange capacity with fertilization were determined. The CEC of the soils was closely related to their organic C contents. Moreover, pseudo- and mobile metal (Cd, Cu, Mn, Pb, Zn) contents in the soils increased due to application of 14 years of mineral fertilizer treatments (N, P, NP, and NPK) when compared to control plots. Eight years after termination of the fertilization in the soil samples taken from soil profiles of the fertilized plots (NPK) for monitoring the residual effects of the fertilizer application, a decrease of 82.6, 54.2, 48.5, 74.4, and 56.9% in pseudo-total Cd, Cu, Mn, Pb, and Zn contents, respectively, was determined.

ISOLATION AND IDENTIFICATION OF RHIZOSPHERIC BACTERIA ASSOCIATED WITH LEMONGRASS FOR POTENTIAL BIOREMEDIATION

2015 ◽  
Vol 77 (24) ◽  
Author(s):  
Najwa Husna Sanusi ◽  
Phang Ing Chia ◽  
Noor Faizul Hadry Nordin
Keyword(s):  
Future Research ◽  
Rrna Gene ◽  
Public Attention ◽  
Isolation And Identification ◽  
Severe Problem ◽  
Rhizospheric Bacteria ◽  
Large Numbers ◽  
Degrading Bacteria ◽  
Cleaner Technology ◽  
Remediation Process

Contamination of soil and groundwater pollution is a severe problem, has been attracting considerable public attention over the last decades. With the demand for green and cleaner technology for remediation process, there is an increased interest in moving away from conventional technologies towards bioremediation technologies. Rhizospheric zone is a suitable place for harboring bacteria that are capable to utilize chemical compounds which will be used either to facilitate growth of bacteria or the host plants. Identification of the specific microbial members should allow for better strategies to enhance biodegradation. This study aimed to isolate and identify the rhizospheric associated microbes of lemongrass (Cymbopogon citratus), a plant that commonly available in South East Asia, which could be used in future research on degradation studies of dibenzofuran. This probably is due to their ability to harbor large numbers of bacteria on their highly branched root systems. A total of 68 strains of dibenzofuran (DF)- degrading bacteria isolated from the rhizospheric soil of lemon grass from 2 different unpolluted sites were characterized. The isolates showed the ability to utilize dibenzofuran as the sole carbon and energy source up to 40 ppm. Identification of the isolates based on 16S rRNA gene sequence assigned them as members of the phyla Proteobacteria and Firmicutes, among which those of the genera, Proteobacteria were most abundant. The presented results indicated the potential of these bacterial isolates in bioremediation of dibenzofuran-contaminated soil.

scholarly journals A high Mn(II)-tolerance strain, Bacillus thuringiensis HM7, isolated from manganese ore and its biosorption characteristics

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8589 ◽  
Author(s):  
Huimin Huang ◽  
Yunlin Zhao ◽  
Zhenggang Xu ◽  
Yi Ding ◽  
Xiaomei Zhou ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Contact Time ◽  
Indoleacetic Acid ◽  
Removal Rate ◽  
Soil Samples ◽  
Manganese Ore ◽  
High Concentration ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
Metal Absorption

Microorganisms play a significant part in detoxifying and immobilizing excessive metals. The present research isolated a strain (HM7) with high Mn(II) tolerance from Mn(II)-contaminated soil samples. The 16S rDNA sequence analysis showed that HM7 had a 99% similarity to Bacillus thuringiensis, which can survive under a high concentration 4,000 mg/L of Mn(II), and the highest removal rate was up to 95.04% at the concentration of 400 mg/L. The highest Mn(II) removal rate was detected at the contact time 72 h, temperature 30 °C, and pH 5.0, while the differences in strain growth and Mn(II) removal rate among different inoculation doses were insignificant. Scanning electron microscopy indicated B. thuringiensis HM7 cells appeared irregular and cracked under Mn(II) stress. Fourier transform infrared exhibited that functional groups like carboxyl, hydroxyl, amino, sulfhydryl groups, and amide bands might take part in the complexation of Mn(II). In addition, HM7 suggested the ability of indoleacetic acid production, siderophore production, and P’ solubilization potential. Therefore, HM7 might have a potential to promote metal absorption by changing the form of heavy metals, and the experiments supported the application of B. thuringiensis HM7 as a biological adsorbent in Mn(II) contaminated environment remediation.

ISOLATION AND IDENTIFICATION OF 3-CHLOROPROPIONIC ACID DEGRADING BACTERIUM FROM MARINE SPONGE

2015 ◽  
Vol 77 (25) ◽  
Author(s):  
Nurul Hanani Mohd Sufian ◽  
Mohd Azrul Naim ◽  
Tengku Haziyamin Tengku Abdul Hamid ◽  
Fahrul Huyop ◽  
Azzmer Azzar Abdul Hamid
Keyword(s):  
Marine Sponge ◽  
Chloride Ion ◽  
Rrna Gene ◽  
Active Components ◽  
Ion Release ◽  
Isolation And Identification ◽  
16S Rrna Gene Analysis ◽  
Bacteria Growth ◽  
Degrading Bacterium ◽  
Degrading Bacteria

Synthetic haloalkanoic acids often applied as active components in herbicides are toxic to the environment and harmful to the living organisms. These compounds are widely released to the environment due to their routine use by agricultural activities.  In recent years, accumulation of haloalkanoic acids was emerged in marine environment. In this study, a 3CP-degrading bacterium which designated as strain H4 was successfully isolated from marine sponge Gelliodes sp. that capable of degrading 3CP as the sole carbon and energy source. The bacteria growth on solid minimal media containing 3CP was the evident for the presence of dehalogenase enzyme. In liquid medium, the doubling time of the cells for strain H4 was 56.82 ± 0.1 h while the maximum chloride ion release was 2.03 ± 0.01 mM. The 16S rDNA sequence of strain H4 was obtained via 16s rRNA gene analysis (1000 bp) and it was closely related to Bacillus aryabhattai B8W22 (99% similarity). To the best of knowledge, this report is the first report detailing haloalkanoic acid degrading bacteria from marine sponge in coastal area of Malaysia.

Identification of large linear plasmids in Arthrobacter spp. encoding the degradation of quinaldine to anthranilate

Microbiology ◽  
2005 ◽  
Vol 151 (2) ◽  
pp. 491-500 ◽  
Author(s):  
Jörg Overhage ◽  
Sonja Sielker ◽  
Stefan Homburg ◽  
Katja Parschat ◽  
Susanne Fetzner
Keyword(s):  
Southern Hybridization ◽  
Sole Source ◽  
Soil Samples ◽  
Linear Plasmid ◽  
Linear Plasmids ◽  
16S Rdna Sequence ◽  
Sequence Identity ◽  
16S Rdna Sequence Analysis ◽  
Genes Encoding ◽  
Arthrobacter Nicotinovorans

Arthrobacter nitroguajacolicus Rü61a, which utilizes quinaldine as sole source of carbon and energy, was shown to contain a conjugative linear plasmid of approximately 110 kb, named pAL1. It exhibits similarities with other linear plasmids from Actinomycetales in that it has proteins covalently attached to its 5′ ends. Southern hybridization with probes for the genes encoding quinaldine 4-oxidase and N-acetylanthranilate amidase indicated that pAL1 contains the gene cluster encoding the degradation of quinaldine to anthranilate. A mutant of strain Rü61a that had lost pAL1 indeed could not convert quinaldine, but was still able to grow on anthranilate. Conjugative transfer of pAL1 to the plasmid-less mutant of strain Rü61a and to Arthrobacter nicotinovorans DSM 420 (pAO1) occurred at frequencies of 5·4×10−4 and 2·0×10−4 per recipient, respectively, and conferred the ability to utilize quinaldine. Five other quinaldine-degrading Gram-positive strains were isolated from soil samples; 16S rDNA sequence analysis suggested the closest relationship to different Arthrobacter species. Except for strain K2-29, all isolates contained a pAL1-like linear plasmid carrying genes encoding quinaldine conversion. A 478 bp fragment that on pAL1 represents an intergenic region showed 100 % sequence identity in all isolates harbouring a pAL1-like plasmid, suggesting horizontal dissemination of the linear plasmid among the genus Arthrobacter.

scholarly journals Isolation and Identification of Hydrocarbon Degradation Bacteria and Phosphate Solubilizing Bacteria in Oil Contaminated Soil in Bojonegoro, East Java, Indonesia

2019 ◽  
Vol 4 (1) ◽  
pp. 134
Author(s):  
Yuni Sri Rahayu
Keyword(s):  
Organic Compounds ◽  
Contaminated Soil ◽  
Soil Samples ◽  
Identification System ◽  
Phosphate Solubilizing Bacteria ◽  
Bacterial Cells ◽  
Isolation And Identification ◽  
Degrading Bacteria ◽  
Phosphate Solubilizing ◽  
Physiological Tests

Petroleum is a mixture of hydrocarbon complexes with organic compounds from sulfur, oxygen, nitrogen and metal-containing compounds. These organic compounds can be used as substrate for bacterial growth. This study aimed to isolate and identify hydrocarbon degrading bacteria and phosphate solubilizing bacteria in oil-contaminated soil in Bojonegoro. This study used an exploration method to find each of the two types of hydrocarbon degrading bacteria and phosphate solubilizing bacteria from soil samples in Bojonegoro that contaminated by oil. Identification of isolates bacterial included macroscopic observations of bacteria, gram staining on bacterial cells and physiological tests. Macroscopic observations include the form of colonies, colony diameter, colony color, colony edge, and elevation. The physiological test using Microbact Identification System to determine the physiological characteristics of bacteria so that genera and types of bacteria can be known. The identification of organisms was based on changes in pH and use of the substrate. The results of data analysis were obtained from five types of bacteria from soil samples that contaminated by oil which were successfully isolated. After identification of species was done, four species of bacteria were obtained, namely Pseudomonas pseudomallei, Pseudomonas fluorescens-25, Flavobacterium odoratum, and Enterococcus sp.

scholarly journals Information on the Distribution of Cadmium in Agricultural Land in the Middle of the Serayu Watershed

2021 ◽  
Author(s):  
Poniman Poniman ◽  
Anik Hidayah ◽  
Sukarjo Sukarjo
Keyword(s):  
Agricultural Land ◽  
Agricultural Soils ◽  
Grid Method ◽  
Threshold Value ◽  
Soil Samples ◽  
Trade Relations ◽  
Average Value ◽  
Trust In Management ◽  
Poor Management

Cadmium (Cd) is a heavy metal that is prohibited in food products. In the long term, consumption of food containing Cd can cause cancer (it is a direct carcinogen in humans). Poor management of the Serayu watershed has resulted in pollution of the surrounding land. Meanwhile, trust in management must be built within the framework of trade in the Industrial Era 4.0, which is developing continuously in world trade relations. This study aimed to obtain information on the distribution of Cd in agricultural soils in the middle of the Serayu watershed in March-August 2017. A total of 220 soil samples were taken using the GRID method, and the analysis of Cd concentration was determined by atomic absorption spectrofotometry (AAS). The results showed that Cd was detected in 142 soil samples in the range of 1.0-<1.5 mg kg−1 and detected in the range of 0.5-<1.0 mg kg−1 in 78 soil samples. The results were non-normally distributed with a clustered distribution pattern. The average value of the distribution of Cd in agricultural land in the middle of Serayu watershed was 1.21 mg kg−1, and the highest Cd concentration was 2.18 mg kg−1. The Cd concentration in the middle of the Serayu watershed is classified within the safe category because the concentration is still below the critical Cd threshold value of 3-8 mg kg−1. Keywords: agricultural land, cadmium, distribution, the middle of Serayu watershed

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