scholarly journals The quality of Etawah crossbreed sperm after sexing with different combination of Bovine Serum Albumin concentrations

2021 ◽  
Vol 888 (1) ◽  
pp. 012025
Author(s):  
S D Rasad ◽  
N Solihati ◽  
K Winangun
Keyword(s):  
Plasma Membrane ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Sperm Quality ◽  
Duncan Multiple Range Test ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Range Test

Abstract This research aimed to determine the quality of Etawah crossbreed sperm after sexing with different combinantion of Bovine Serum Albumin (BSA) concentrations. The parameter of this research were motility, viability, intact plasma membrane (IPM) and intact acrosome cap (IAC) (%). The Completely randomized design (CRD) was applied in this experiment involving 4 treatments of the combination of BSA concentrations (T1=3%:6%, T2 = 4%:8%, T3=5%:10%, T4=6%:12% at upper and lower fraction) and each treatments was repeated 5 times at post chilled and post sexing. Data were analyzed by using analysis of variance (ANOVA), followed by Duncan Multiple Range Test. The result showed that thecombination of BSA concentrations affected (P<0.05) motility, viability, IPM and IAC. The highest value of sperm quality in upper and lower fraction was obtained from the combination BSA of 5%:10% (motility 78.60 ± 2.61% and 73.80 ± 2.49%; viability 282 ± 14.30 and 252.8 ± 12.97 hours; IPM-value 79.60 ± 1.98% and 74.70 ± 1.82% and IAC 81.00 ± 1.46% and 76.90 ± 1.29%). Based on the results it can be concluded that the quality of Etawah crossbreed sperm after sexing is affected by the combination of BSA concentrations.

scholarly journals Penambahan Bovine Serum Albumin pada Beltsville Thawing Solution Dapat Mempertahankan Kualitas Semen Babi yang Disimpan pada 15°C (ADDITION OF BOVINE SERUM ALBUMIN TO BELTSVILLE THAWING SOLUTION COULD MAINTAIN QUALITY OF PIG SEMEN STORED AT 15OC)

2019 ◽  
Vol 20 (3) ◽  
pp. 330
Author(s):  
Wayan Bebas ◽  
Wayan Gorda
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Plasma Membranes ◽  
Treatment Groups ◽  
Progressive Motility ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Optimal Quality

This study aims to maintain the quality of pig semen for longer during storage at 15oC, in an effort to support artificial insemination programs with the addition of Bovine Serum albumin (BSA) to diluent Beltsville Thawing Solution (BTS). This study uses a completely randomized design with five treatment groups, each To = semen was diluted with BTS without the addition of BSA ; T1 = with the addition of 5 mg BSA/mL diluent; T2 = with the addition of 10 mg BSA/mL diluent; T3 = with the addition of 15 mg BSA/mL diluent; T4 = with the addition of 20 mg BSA/mL diluent. Each treatment was repeated five times so that the number of samples used was twenty-five. The diluted cement is stored at 15oC for 72 hours then observing the quality of cement includes: progressive motility, dead spermatozoa, abnormalities, and intact plasma membranes. The data obtained were analyzed by analysis of variance, if there were differences followed by Duncan’s test. The results showed, addition of BSA concentration of 10 mg/mL and 15 mg/mL of diluent gives the same effect on the quality of cement during storage and significantly better (p <0.05) when compared to the addition of 0 mg/mL, 5 mg/mL and 20 mg/mL diluents. It can be concluded, the addition of BSA 10 mg/mL BTS diluents can maintain the most optimal quality of pig semen against progressive motility, dead spermatozoa, abnormalities and intact plasma membranes.

scholarly journals PENGGANTIAN BOVINE SERUM ALBUMIN PADA PENGENCER CEP-2 DENGAN SERUM DARAH SAPI DAN PUTIH TELUR TERHADAP KUALITAS SEMEN CAIR SAPI LIMOUSIN SELAMA PENDINGINAN (The Substitution of Bovine Serum Albumin with Cattle Blood Serum and Egg White in CEP-2 Diluent on the Quality of Limousin Bull Liquid Semen during Refrigerating)

2016 ◽  
Vol 10 (2) ◽  
pp. 98-102
Author(s):  
Trinil Susilawati ◽  
Feri Eka Wahyudi ◽  
Inna Anggraeni ◽  
Nurul Isnaini ◽  
Muhammad Nur Ihsan
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Blood Serum ◽  
Sperm Motility ◽  
Bovine Serum ◽  
Sperm Quality ◽  
Egg Yolk ◽  
Egg White ◽  
Cattle Blood

This study aims to determine the effect of the substitution of bovine serum albumin (BSA) with cattle blood serum and egg white in the diluent of Cauda epididymal Plasma 2 (CEP-2) on sperm quality of Limousin cattle during cooling at 3-5 C. The research material used was rejected Limousin bull sperm (motility of 50-60%) from Artificial Insemination Centre Singosari, Malang. This research was a laboratory experiment using a randomized block design which was composed of six treatments with 10 replications, those were T0 as controls ((90% CEP-2 with BSA + 10% egg yolk); T1 (83.84% CEP-2 + 6.16% cattle blood serum + 10% egg yolk); T2 (81.84% CEP-2 + 8.16% cattle blood serum + 10% egg yolk); dan T3 (90% CEP-2 + 0,4% egg white + 10% egg yolk); T4 (90% CEP-2 + 0.8% egg white + 10% egg yolk); and T5 (90% CEP-2 without BSA + 10% egg yolk). Parameters measured were the percentage of motility, viability, and abnormality of sperms. Results of research after 48 hours of storage showed that the percentage of sperm motility in T0, T1, T2, T3, T4, and T5 were 40.50±5.90, 36±36.16, 34.00±6.58, 40.50±3.69, 38.50±3.37, and 38.50±4.12, respectively, while the percentage of sperms viability were 75.16±4.30, 70.50±2.88, 73.80±2.80, 74.80±3.30, 75.13±3.13, and 74.03±4.13, respectively, and the percentage of sperms abnormality were 10.14±2.34, 10.62±1.34, 11.33±2.00, 10.94±2.82, 10.02±1.95, and 10.78±1.96, respectively. In conclusion, CEP-2 diluent with or without the addition of 19% egg yolk in BSA and the substitution of BSA with 0.4-0.8% egg white can maintain semen quality to hour of 48 in cold storage.This study aims to determine the effect of the substitution of bovine serum albumin (BSA) with cattle blood serum and egg white in the diluent of Cauda epididymal Plasma 2 (CEP-2) on sperm quality of Limousin cattle during cooling at 3-5 C. The research material used was rejected Limousin bull sperm (motility of 50-60%) from Artificial Insemination Centre Singosari, Malang. This research was a laboratory experiment using a randomized block design which was composed of six treatments with 10 replications, those were T0 as controls ((90% CEP-2 with BSA + 10% egg yolk); T1 (83.84% CEP-2 + 6.16% cattle blood serum + 10% egg yolk); T2 (81.84% CEP-2 + 8.16% cattle blood serum + 10% egg yolk); dan T3 (90% CEP-2 + 0,4% egg white + 10% egg yolk); T4 (90% CEP-2 + 0.8% egg white + 10% egg yolk); and T5 (90% CEP-2 without BSA + 10% egg yolk). Parameters measured were the percentage of motility, viability, and abnormality of sperms. Results of research after 48 hours of storage showed that the percentage of sperm motility in T0, T1, T2, T3, T4, and T5 were 40.50±5.90, 36±36.16, 34.00±6.58, 40.50±3.69, 38.50±3.37, and 38.50±4.12, respectively, while the percentage of sperms viability were 75.16±4.30, 70.50±2.88, 73.80±2.80, 74.80±3.30, 75.13±3.13, and 74.03±4.13, respectively, and the percentage of sperms abnormality were 10.14±2.34, 10.62±1.34, 11.33±2.00, 10.94±2.82, 10.02±1.95, and 10.78±1.96, respectively. In conclusion, CEP-2 diluent with or without the addition of 19% egg yolk in BSA and the substitution of BSA with 0.4-0.8% egg white can maintain semen quality to hour of 48 in cold storage.

scholarly journals Pemanfaatan Kotoran Ayam untuk Peningkatan Kualitas Pucuk Tebu Sebagai Pakan Ruminasia

2018 ◽  
Vol 21 (1) ◽  
pp. 33
Author(s):  
C I Sutrisno ◽  
B.W.H.E Prasetyono ◽  
E Ali
Keyword(s):  
Analysis Of Variance ◽  
Sugar Cane ◽  
Research Result ◽  
Duncan Multiple Range Test ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Two Stages ◽  
Range Test ◽  
Better Than

<p>Experiment was carried out in two stages. The first was aimed to study the viabilty of microbe of dried-oven manure and dried-sunshine manure. Completely Randomized Design (CDR), 3 treatments and 4 replications, was used throughout the experiment with content of manure microbe as the parameters. The research result indicates that the way of draining degraded the viability of microbia manure. Manure microbia’s viability of dried-oven manure was better than that of the dried-sunshine manure.</p>The second was aimed to investigate the effect of dried-manure as starter with different time of fermentation on the quality of fermented sugar cane. Completely Randomized Design, factorial pattern 3x3 by 3 replications was employed during the experiment. The first factor was starter levels (A): 0, 15 and 30%, while the second was different time of fermentation (W): 0, 4 dan 8 week. Parameter perceived by fermented fiber component, which analysis by Analysis of Variance, while the differences among the treatment were tested with the Duncan Multiple Range Test (DMRT), continued by Polinomial Ortogonal. Fermented sugar cane by dry manure with different time of fermentation degraded the rate of NDF and ADF. The best performance was reached at 30% of starter and 4 weeks of fermentation.

scholarly journals Quality Of Duck Eggs At Different Soaking Times In Noni Leaf Extract Stored For 15 Days

2021 ◽  
Vol 3 (2) ◽  
pp. 85
Author(s):  
Sri Sukaryani ◽  
Engkus Ainul Yakin ◽  
Hardika Eka Rhamadanu
Keyword(s):  
Leaf Extract ◽  
Egg Quality ◽  
Chemical Compounds ◽  
Duncan Multiple Range Test ◽  
Extract Concentration ◽  
The People ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Range Test

 Duck eggs are one type of protein source food that is much favored by the people of Indonesia.  However, currently there are not many ways of preservation to maintain the quality of duck eggs in storage.  With preservation by immersion with noni leaf extract, it can be an alternative in maintaining the quality of duck eggs in storage for 15 days.  This is because noni leaves contain elements of chemical compounds such as amino acids, phenolic compounds, ursulic acid, alkaloids, phenols, and glycosides which are antimicrobial, antibacterial and anti-inflammatory.  This study aims to determine the effect of soaking duck eggs in noni leaf extract on the interior quality of duck eggs.  This study used a completely randomized design (CRD) with a concentration of 45% noni leaf extract with immersion time of 0, 12, 24, 36 and 48 hours with 3 replications.  To determine whether there is a difference in treatment using Duncan Multiple Range Test (DMRT) at the level of = 0.05.  The research variables are Albumen Insex, Yolk Index and Haugh Index.  Data were analyzed by SPSS.  The results of the study concluded that soaking duck eggs with noni leaf extract for 12-48 hours with an extract concentration of 45% had no significant effect on egg quality in this case the yolk index, albumen index and Haugh index.

scholarly journals Evaluasi fertilitas, daya tetas, dan kualitas DOD dari itik Alabimaster, Mojomaster, dan Mojomaster x Alabimaster

2021 ◽  
Vol 19 (2) ◽  
pp. 171
Author(s):  
Tatan Kostaman ◽  
Dewi Sari Kumalawati ◽  
Soni Sopiyana ◽  
Maijon Purba
Keyword(s):  
Duncan Multiple Range Test ◽  
Normal Range ◽  
Average Data ◽  
Cage Complex ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Hatching Eggs ◽  
Average Fertility ◽  
Range Test

<p class="MDPI17abstract"><strong>Objective: </strong>The evaluating of the fertility, hatchability, and DOD quality of the ducks selected by the Indonesian Research Institute for Animal Production (Balitnak) consisting of Alabimaster, Mojomaster, and Mojomaster x Alabimaster (MASTER) ducks.</p><p class="MDPI17abstract"><strong>Methods: </strong>The research was conducted at the Ducks Cage Complex, Balitnak. Using 9,388 Alabimaster duck eggs, 1,635 Mojomaster duck eggs, and 7,638 Mojomaster x Alabimaster (MASTER) duck eggs. The variables observed included fertility, hatchability, and DOD quality. The DOD criteria chosen were healthy, agile, not blind, not disabled, not weak, not wet, and not omphalitis. Completely Randomized Design (CRD) was used and the calculation of the average data for all variables was processed with the help of the SPSS 25.0 program. If there is a difference in value, then the test is carried out with the Duncan Multiple Range Test.<strong></strong></p><p class="MDPI17abstract"><strong>Results: </strong>The fertility percentage of Alabimaster, Mojomaster, and MASTER ducks did not show any difference (P&gt;0.05) but seen from the relatively high percentage value because it gave a figure of 94%. The percentage of hatchability was significantly different (P&lt;0.05) for the three ducks. The percentage of DOD that passed the selection, MASTER ducks gave a higher value than the other two ducks. The ratio of male and female cubs for the three ducks showed that there were more male than female cubs, but still within the normal range.<strong></strong></p><strong>Conclusions: </strong>The average fertility, hatchability, and DOD selected from the hatching eggs of Alabimaster, Mojomaster, and MASTER ducks resulted from the research being quite good.

scholarly journals Kualitas Organoleptik Telur Itik yang Diberi Pakan Maggot Hidup (Hermetia illucens)

2019 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Aam Gunawan
Keyword(s):  
High Protein ◽  
Protein Source ◽  
Hermetia Illucens ◽  
Organoleptic Quality ◽  
Low Protein ◽  
Randomized Design ◽  
Living State ◽  
Completely Randomized Design ◽  
Range Test

Maggot black soldier fly (Hermetia illucens) is a protein source of feed which is highly favored by poultry, especially ducks and chickens. However, it is feared that the provision of live maggot in ducks will affect the organoleptic quality of the egg, especially its taste and aroma. Therefore this study aims to determine the organoleptic quality of duck eggs fed Hermetia illucens maggot feeds in a living state. The study used 120 alabio ducks which were placed in a postal cage. The design used was a completely randomized design, each treatment using six replications. Each replication is taken egg sample to be tested panelists. The panelists used were 67 panelists who were somewhat trained. The data obtained were analyzed of variance and Duncan's multiple range test. The treatments that were tried consisted of P1: low protein rations without live maggot, P2: low protein rations with live maggot administration 40 g/bird/day, P3: high protein rations without live maggot administration, and P4: high protein rations with live maggot administration 40g/bird/day. The results showed that the treatment affected the texture, flavor, and aroma of boiled eggs. Ducks fed with high protein ration coupled with the provision of live maggot 40 g/bird/day produce softer textures, tastes quite good, and aroma more fishy. Keywords: Maggot, duck eggs, organoleptics

scholarly journals THE ADDITION OF COCONUT WATER AND THIAMINE TOWARDS CHRYSSANTHENUM MICRO CUTTINGS (Dendranthema grandiflora Tzvelev) IN VITRO

Agrivet ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 39
Author(s):  
Salma Nabila ◽  
Endah Budi Irawati ◽  
Rina Srilestari
Keyword(s):  
Water Concentration ◽  
Coconut Water ◽  
Fresh Weight ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Range Test ◽  
Growing Shoot

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety  of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on  fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

scholarly journals Kandungan Total Bakteri dan Total Fungi pada Pellet Limbah Penetasan yang Dibuat dengan Penambahan Bentonit

2016 ◽  
Vol 16 (2) ◽  
pp. 69
Author(s):  
Aprilian Adi Nugroho ◽  
Sri Sumarsih ◽  
Bambang Sulistiyanto
Keyword(s):  
Analysis Of Variance ◽  
Total Content ◽  
Duncan Multiple Range Test ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Bacteria And Fungi ◽  
Total Fungi ◽  
Number Of Bacteria ◽  
Total Bacteria ◽  
Range Test

ABSTRAK. Penelitian efek penambahan bentonit dalam proses pelleting terhadap total bakteri dan total fungi dari produk pellet limbah penetasan sebagai bahan pakan alternatif, telah dilaksanakan di Laboratorium Teknologi Pakan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro. Penambahan bentonit pada proses pelleting diharapkan mengurangi total bakteri dan total fungi pada pellet olahan limbah penetasan, sehingga aman digunakan sebagai bahan pakan alternatif. Pada penelitian ini limbah penetasan yang berupa cangkang telur, telur infertile, telur gagal menetas, serta DOC afkir dan mati dihancurkan, dicampur dengan bentonit dan dibuat pellet, untuk kemudian dianalisis kandungan total bakteri dan total fungi pada produk pellet. Perlakuan bentonit dilakukan dengan aras 0, 2, 4 dan 6% (B/B). Penelitian dilakukan dengan rancangan acak lengkap (RAL) 4 perlakuan dan 5 ulangan. Data dianalisis dengan analisis ragam dan dilanjutkan dengan uji wilayah ganda Duncan untuk mengetahui perbedaan antar perlakuan. Hasil menunjukkan bahwa pemberian bentonit dari berbagai level berpengaruh sangat nyata (P<0,01). Pemberian bentonit pada tingkat tertinggi (6%) mampu menekan total bakteri dan total fungi pada kisaran 105 cfu/g. Disimpulkan bahwa bentonit mampu menekan kandungan total bakteri dan total fungi pada produk pellet limbah penetasan. (Numbers of total bacteria and total fungi of hatchery waste pellets that made by adding bentonite) ABSTRACT. A Research about the effect of bentonite addition in the pelletizing process on total bacteria and total fungi of pellet product of hatchery waste as an alternative feedstuff, was done at Feed Technology Laboratory, Faculty of Animal and Agricultural Sciences, Diponegoro University. The addition of bentonite was expected to reduce the number of bacteria and fungi in the pellet product of hatchery waste, therefore, it could be used safely as an alternative feedstuff. In this experiment, the hatchery waste in the forms of eggshell, infertile eggs, un-hatched eggs, dead and culled DOC were blended, mixed with bentonite and pelleted,and then the number of total bacteria and total fungi of pellet was analyzed. The treatment was carried out with the level of bentonite i.e. 0, 2, 4 and 6% (w/w). Completely randomized design (CRD)was applied by 4 treatments and 5 replications of each. Data analyzed by analysis of variance (ANOVA), and followed by Duncan multiple range test (DMRT) to determine differences among the treatments. The results showed that administration of bentonite at various levels significantly affect the number of bacteria and fungi in the hatchery waste pellets (P <0.01). Administration of bentonite at the level of 6% shows the highest value on suppressing the content of total bacteria as well as fungi up to the range of total content 105cfu/g. In conclusion, the bentonite was able to suppress the total amount of bacteria and total fungi in the hatchery waste pellets.

Effect of Bovine Serum Albumin On Quality of Indian Red Jungle Fowl Spermatozoa

Cryobiology ◽  
2021 ◽  
Vol 103 ◽  
pp. 182
Author(s):  
Bushra Rakha ◽  
Muhammad Ansari ◽  
Shamim Akhter ◽  
Julian Santiago-moreno
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Red Jungle Fowl
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