scholarly journals Disease surveillance of cultured marine fish in the North of Bali, Indonesia

2021 ◽  
Vol 890 (1) ◽  
pp. 012035
Author(s):  
K Mahardika ◽  
I Mastuti ◽  
D Syahidah ◽  
S Ismi ◽  
Zafran
Keyword(s):  
Marine Fish ◽  
Disease Surveillance ◽  
High Concentration ◽  
Bacterial Populations ◽  
The North ◽  
Fish Samples ◽  
Cultured Fish ◽  
Net Cages ◽  
Bacterial Concentrations ◽  
Very High

Abstract A regular surveillance of marine fish diseases was conducted from March to November 2019 in order to determine the occurrence time of the diseases within the mariculture centre of the North of Bali, Indonesia. The monthly surveillance was conducted by collecting 15 fish samples from each of the three hatcheries in Gerokgak and Penyabangan villages and of the two floating net cages in Pegametan Bay, Sumberkima village. Bacterial concentrations were grouped into 4 categories including low, moderate, high and very high. Surveillance data were analyzed using bivariate descriptive statistical methods. The results showed some important findings. First, the results of the study showed that NNV infection was found during the transitional seasons in March to June and September to November. Parasite infection were more frequent observed in fish with high and very high bacterial population. Second, high concentration of total bacteria in fish-feces occurred throughout the year. The prevalence of NNV infection and bacterial populations at the high to very high concentration were mostly occurred in the cultured fish in hatcheries at the size of 1-10 g, while in cultured fish at the net cages were mostly occurred at the size of > 50 g.

scholarly journals Characterization of culturable bacterial communities associated with cage cultured fish under different stocking densities in Setiu Wetlands, Terengganu, Malaysia.

2021 ◽  
Author(s):  
Mohammed Salah M.Tawfeeq
Keyword(s):  
Bacterial Species ◽  
Drug Application ◽  
Stocking Density ◽  
Water Clarity ◽  
Quality Parameters ◽  
Study Objective ◽  
Significant Difference ◽  
Fish Samples ◽  
Cultured Fish ◽  
Net Cages

Abstract The study objective was to evaluate the effects of fish stocking density on the prevalence of pathogens isolated from sixty clinically healthy fishes reared under different densities in floating net-cages in Setiu lagoon. The water temperature, pH, DO, TDS, salinity, water clarity, depth, and coliform concentration, were all determined within the fish cages. The healthy fish samples were randomly collected from 3 sites in cages with low and high stocking densities at each site. The bacteria were isolated from the skin, gills, kidneys, and liver of each fish sample, followed by identification to species level using the VITEK-2 system. The pathogens with beta-hemolysis characteristics were selected for antibiotic susceptibility against the following drugs: AM-10 µg, P-10 U, CL-30 µg, TC-30 µg, CP-5 µg, GM-10 µg, KM-30µg, and SM-10 µg. Water quality parameters showed no differences between the cages of low and high fish densities except for site C. The total number of isolates, microbial species, and the number of pathogens isolated from fish revealed no significant difference between the fish stocked in low and high densities. A total of 25 bacterial species were isolated, which included 14 gram-positive and 11 gram-negative. The (SM) drug application is suspected on this farm.

Preliminary results of the study on the reasons of the Hai Hau erosion phenomenon

2007 ◽  
Vol 29 (3) ◽  
pp. 415-426
Author(s):  
Pham Van Ninh ◽  
Phan Ngoc Vinh ◽  
Nguyen Manh Hung ◽  
Dinh Van Manh
Keyword(s):  
Mathematical Modeling ◽  
Historical Data ◽  
Middle Part ◽  
Red River ◽  
Erosion Process ◽  
Red River Delta ◽  
The North ◽  
Severe Erosion ◽  
North And South ◽  
Very High

Overall the evolution process of the Red River Delta based on the maps and historical data resulted in a fact that before the 20th century all the Nam Dinh coastline was attributed to accumulation. Then started the erosion process at Xuan Thuydistrict and from the period of 1935 - 1965 the most severe erosion was contributed in the stretch from Ha Lan to Hai Trieu, 1965 - 1990 in Hai Chinh - Hai Hoa, 1990 - 2005 in the middle part of Hai Chinh - Hai Thinh (Hai Hau district). The adjoining stretches were suffered from not severe erosion. At the same time, the Ba Lat mouth is advanced to the sea and to the North and South direction by the time with a very high rate.The first task of the mathematical modeling of coastal line evolution of Hai Hau is to evaluate this important historical marked periods e. g. to model the coastal line at the periods before 1900, 1935 - 1965; 1965 - 1990; 1990 - 2005. The tasks is very complicated and time and working labors consuming.In the paper, the primarily results of the above mentioned simulations (as waves, currents, sediments transports and bottom - coastal lines evolution) has been shown. Based on the obtained results, there is a strong correlation between the protrusion magnitude and the southward moving of the erosion areas.

Ground water quality of selected areas of Punjab and Sind Provinces, Pakistan: Chemical and microbiological aspects

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Ionic Concentration ◽  
Chloride Ions ◽  
Potassium Ions ◽  
Ammonium Ions ◽  
High Concentration ◽  
Phosphate Ions ◽  
Sodium Magnesium ◽  
Very High ◽  
Microbiological Aspects

The assessment of groundwater is essential for the estimation of suitability of water for safe use. An attempt has been made to study the groundwater of selected areas of Punjab (Sheikhupura & Sahiwal) and Sindh (Sindh, Jawar Dharki and Dharki), Pakistan. The results indicate that pH, color and odor were all within limits of WHO that is pH ranges 6.5–8.5, colorless and odorless, respectively. The high values of suspended solids were observed in the Sindh-1 and Dharki samples. Microbiologically only Sahiwal and Jawar Dharki were found fit for drinking purpose. Trace metals analysis of Sheikhupura-1 and Sindh-1 showed that values do not fall within limits of WHO for Iron. The ionic concentration analysis showed that high bicarbonate (HCO3-), ions are present in the samples of Sahiwal and Dharki; Sindh-1 and Jawar Dharki samples showed very high concentration for chloride ions, all samples were satisfactory level for sulphate (SO42-), sodium, magnesium and phosphate ions except samples of Sindh-1 and Jawar Dharki. High concentration of calcium and potassium ions was observed in samples of Sindh-1, while all other samples were found fit for drinking purposes in respect of nitrate, nitrite and ammonium ions. The high concentration of Fluoride was found only in Sheikhupura-2 samples.

scholarly journals Near quantitative synthesis of urea macrocycles enabled by bulky N-substituent

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yingfeng Yang ◽  
Hanze Ying ◽  
Zhixia Li ◽  
Jiang Wang ◽  
Yingying Chen ◽  
...  
Keyword(s):  
High Efficiency ◽  
Molecular Structures ◽  
High Yield ◽  
Kinetic Control ◽  
High Concentration ◽  
Weak Association ◽  
Quantitative Synthesis ◽  
Thermodynamic Stabilization ◽  
Discrete Structures ◽  
Very High

AbstractMacrocycles are unique molecular structures extensively used in the design of catalysts, therapeutics and supramolecular assemblies. Among all reactions reported to date, systems that can produce macrocycles in high yield under high reaction concentrations are rare. Here we report the use of dynamic hindered urea bond (HUB) for the construction of urea macrocycles with very high efficiency. Mixing of equal molar diisocyanate and hindered diamine leads to formation of macrocycles with discrete structures in nearly quantitative yields under high concentration of reactants. The bulky N-tert-butyl plays key roles to facilitate the formation of macrocycles, providing not only the kinetic control due to the formation of the cyclization-promoting cis C = O/tert-butyl conformation, but also possibly the thermodynamic stabilization of macrocycles with weak association interactions. The bulky N-tert-butyl can be readily removed by acid to eliminate the dynamicity of HUB and stabilize the macrocycle structures.

Small-volume vitrification and rapid warming yield high survivals of one-cell rat embryos in cryotubes

2021 ◽  
Author(s):  
Yasuyoshi Fukuda ◽  
Misako Higashiya ◽  
Takahiro Obata ◽  
Keita Basaki ◽  
Megumi Yano ◽  
...  
Keyword(s):  
Cooling Rate ◽  
Small Volume ◽  
Sucrose Solution ◽  
High Concentration ◽  
Rat Embryos ◽  
Low Concentrations ◽  
Intracellular Ice Formation ◽  
Intracellular Ice ◽  
Warming Rate ◽  
Very High

Abstract To cryopreserve cells, it is essential to avoid intracellular ice formation during cooling and warming. One way to achieve this is to convert the water inside the cells into a non-crystalline glass. It is currently believed that to accomplish this vitrification, the cells must be suspended in a very high concentration (20–40%) of a glass-inducing solute, and subsequently cooled very rapidly. Herein, we report that this belief is erroneous with respect to the vitrification of one-cell rat embryos. In the present study, one-cell rat embryos were vitrified with 5 μL of EFS10 (a mixture of 10% ethylene glycol, 27% Ficoll, and 0.45 M sucrose) in cryotubes at a moderate cooling rate, and warmed at various rates. Survival was assessed according to the ability of the cells to develop into blastocysts and to develop to term. When embryos were vitrified at a 2,613 °C/min cooling rate and thawed by adding 1 mL of sucrose solution (0.3 M, 50 °C) at a warming rate of 18,467 °C/min, 58.1 ± 3.5% of the EFS10-vitrified embryos developed into blastocysts, and 50.0 ± 4.7% developed to term. These rates were similar to those of non-treated intact embryos. Using a conventional cryotube, we achieved developmental capabilities in one-cell rat embryos by rapid warming that were comparable to those of intact embryos, even using low concentrations (10%) of cell-permeating cryoprotectant and at low cooling rates.

scholarly journals Torrential Flood Water Management: Rainwater Harvesting through Relation Based Dam Suitability Analysis and Quantification of Erosion Potential

2021 ◽  
Vol 10 (1) ◽  
pp. 27
Author(s):  
Bilal Ahmad Munir ◽  
Sajid Rashid Ahmad ◽  
Raja Rehan
Keyword(s):  
Sediment Yield ◽  
Soil Loss ◽  
Rainwater Harvesting ◽  
Suitability Analysis ◽  
Valley Bottom ◽  
The North ◽  
Roughness Index ◽  
Dam Site ◽  
Very High

In this study, a relation-based dam suitability analysis (RDSA) technique is developed to identify the most suitable sites for dams. The methodology focused on a group of the most important parameters/indicators (stream order, terrain roughness index, slope, multiresolution valley bottom flatness index, closed depression, valley depth, and downslope gradient difference) and their relation to the dam wall and reservoir suitability. Quantitative assessment results in an elevation-area-capacity (EAC) curve substantiating the capacity determination of selected sites. The methodology also incorporates the estimation of soil erosion (SE) using the Revised Universal Soil Loss Equation (RUSLE) model and sediment yield at the selected dam sites. The RDSA technique identifies two suitable dam sites (A and B) with a maximum collective capacity of approximately 1202 million m3. The RDSA technique was validated with the existing dam, Gomal-Zam, in the north of Sanghar catchment, where RDSA classified the Gomal-Zam Dam in a very high suitability class. The SE estimates show an average of 75 t-ha−1y−1 of soil loss occurs in the study area. The result shows approximately 298,073 and 318,000 tons of annual average sediment yield (SY) will feed the dam A and B respectively. The SE-based sediment yield substantiates the approximate life of Dam-A and Dam-B to be 87 and 90 years, respectively. The approach is dynamic and can be applied for any other location globally for dam site selection and SE estimation.

scholarly journals Q fever in South Australia: an outbreak in a meat-works

1962 ◽  
Vol 60 (1) ◽  
pp. 1-20 ◽  
Author(s):  
Margaret D. Beech ◽  
A. E. Duxbury ◽  
Peter Warner
Keyword(s):  
Q Fever ◽  
South Australia ◽  
Meteorological Conditions ◽  
American Continent ◽  
North American Continent ◽  
The Past ◽  
The North ◽  
Epidemiological Features ◽  
Very High ◽  
Abattoir Workers

This paper consists of an epidemiological study of 52 cases of Q fever occurring in metropolitan Adelaide in 1957 and also a description of the results of a survey of 516 sera obtained from abattoir workers.The only case occurring outside the abattoirs was a dairy farmer who probably became infected while visiting the abattoirs. If this were so the incubation period (35 days) of his disease would have been exceptionally long.The general features of the outbreak, which lasted several months, differed from those on the North American continent in that the latter occurred explosively within a few days with very high attack rates. The situation in the Adelaide abattoirs is similar to that in Brisbane, where the disease appears to be endemic. However, unlike in Adelaide, cases are commonly recognized outside the abattoirs in Brisbane.In the abattoirs the disease affected mainly inspectors, those working on killing beef, and those working on offal. Mutton workers were not so severely affected. However, all these groups had similar incidences of low titre antibodies suggesting that in the past Q fever spread equally in all killing departments. In departments not directly associated with slaughtering the incidence both of cases in 1957 and low titre antibodies was relatively small.It was suggested that the epidemiological features of Q fever in Adelaide could be explained by the irregular appearance of animals from infected herds situated perhaps in Queensland—a known endemic area. Perhaps the appearance of such animals in the Adelaide abattoirs might be governed by meteorological conditions such that they were prevented from going to the ordinarily most convenient slaughterhouse.

A Culture Technique Using Marine Fish Kidney to Obtain Chromosomes

1979 ◽  
Vol 36 (4) ◽  
pp. 458-461 ◽  
Author(s):  
Eun Ho Park ◽  
Sang Dai Park
Keyword(s):  
Chick Embryo ◽  
Marine Fish ◽  
Culture Technique ◽  
Kidney Cells ◽  
High Concentration ◽  
Embryo Extract ◽  
Key Words Kidney ◽  
Chick Embryo Extract ◽  
Conger Eel

A relatively simple and reliable in vitro method for marine fish chromosome study was developed. The addition of 10% chick embryo extract to serum-supplemented Eagle's minimum essential medium with high concentration of NaCl resulted in marked growth of kidney cells in the marine conger eel (Astroconger myriaster) after activation by phytohemagglutinin (PHA). Culture medium without chick embryo extract or PHA and/or with normal concentration of NaCl did not induce substantial growth. In contrast to reports by others, humidified culture was not required for excellent cell growth of these teleost kidney cells. Numerous metaphases unmarred by overlapping chromosomes were recovered and excellent karyograms were available for detailed karyotype analysis. Key words: kidney, culture, marine fish, chromosome

Diffusion In Insect Wing Muscle, The Most Active Tissue Known

1964 ◽  
Vol 41 (2) ◽  
pp. 229-256 ◽  
Author(s):  
TORKEL WEIS-FOGH
Keyword(s):  
Liquid Phase ◽  
Safety Factor ◽  
Metabolic Rates ◽  
High Concentration ◽  
Tracheal System ◽  
Insect Wing ◽  
Main Site ◽  
Normal Diffusion ◽  
Large Fibre ◽  
Very High

1. The tracheal system of insect wing muscle is so dense that between 10-1 and 10-3 of any cut area is occupied by air tubes. In most cases, air tube diffusion of O2 and CO2 through the muscle is therefore several thousand times quicker than diffusion in the liquid phase. 2. In large insects the primary tracheal supply must be strongly ventilated while diffusion is sufficient in the remaining part of the air tubes, even at the highest metabolic rates encountered in any insect. 3. The tracheoles represent the main site of exchange between the gaseous and the liquid phase while the tracheae are of little significance in this respect. The fibres cannot exceed about 20 µ in diameter unless the tracheoles indent the surface and become ‘internal’. 4. Muscular pumping of air and blood due to shortening is of little importance for the exchange of gases but of major importance for the supply with fuel for combustion. However, the large fibre diameters and the tidal nature of the pumping necessitates a very high concentration of fuel in the haemolymph. The high concentration of trehalose in insect blood is considered to be an essential adaptation to flapping flight. 5. The transport by diffusion of O2 and CO2 was followed in detail in a number of concrete examples in the gaseous as well as in the liquid phase. Within a safety factor of 2-3, the rate of transport was always found to be adequate. There is no reason to suggest other mechanisms than a simple, normal diffusion.

Vaporization and Ultra-Fine Particle Generation during the Plasma Spraying Process

1997 ◽  
Author(s):  
K.A. Gross ◽  
P. Fauchais ◽  
M. Vardelle ◽  
J. Tikkanen ◽  
J. Keskinen
Keyword(s):  
Fine Particles ◽  
High Concentration ◽  
Thermal Spray Process ◽  
Particle Generation ◽  
Spray Process ◽  
Ultra Fine Particle ◽  
Fast Cooling ◽  
Ultra Fine Particles ◽  
Plasma Spraying Process ◽  
Very High

Abstract The thermal spray process melts powder at very high temperatures and propels the molten material to the substrate to produce a coherent deposit. This heating produces a certain amount of vaporization of the feedstock. Upon exiting the plasma plume the fast cooling conditions lead to condensation of the vapor. An electrical low pressure impactor was used to monitor the concentration of ultra-fine particles at various radial and axial distances. Metal, namely iron powder, showed very high concentration levels which increase with distance. Ultra-fine particles from ZrO2-8Y2O3 reached a peak concentration at 6 cm. Use of an air barrier during spraying decreases the population of ultra-fine particles facilitating the production of a stronger coating.

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