scholarly journals Potential Of Various Types of Media for Breeding Oyster Mushroom F2

2021 ◽  
Vol 328 ◽  
pp. 08015
Author(s):  
Widiwurjani ◽  
Ida Retno Mulyani ◽  
Ilmatus Sa’diyah ◽  
N.K. Sari
Keyword(s):  
Growth Rate ◽  
Rice Bran ◽  
Oyster Mushroom ◽  
Growth Speed ◽  
Soybean Seeds ◽  
Mycelium Growth ◽  
Randomized Design ◽  
Mycelium Growth Rate ◽  
Materials Used ◽  
Corn Kernels

This research was conducted at the Laboratory of Biotechnology 1 and Farmers' Land in Karanganyar village, Poncokusumo, Malang. The materials used in this study were white oyster mushroom F1 seeds, 70% alcohol, soybean seeds, corn seeds, rice, sawdust, rice bran and mushroom baglog. This study used an experimental design, namely Completely Randomized Design (CRD) with 8 treatment levels B1 = corn kernels (100%), B2 = soybean seeds (100%), B3 = rice (100%), B4 = sawdust (100%), B5 = corn kernels (50%) + sawdust (40%) + rice bran (10%). Parameters observed were height of Mycelium-covered Media (cm), Mycelium Growth Speed (cm/day), Contamination Occurrence Percentage (%). The average yield of the highest seedling height was 12.79 cm in treatment B8. The parameter of mycelium growth rate in B8 treatment had the fastest growth rate of 1.82 cm/ha. The lowest contamination was found in treatment B5, B6 by 4% and B7 by 20%. Treatment B3, B4 and B8 did not experience contamination.

scholarly journals The effect of different substrates on oyster mushroom (Pleurotus ostreatus) spawn growth

2021 ◽  
Vol 911 (1) ◽  
pp. 012044
Author(s):  
R Muslimin ◽  
H Hartono ◽  
R Rachmawaty ◽  
A Ali ◽  
M Junda ◽  
...  
Keyword(s):  
Growth Rate ◽  
Analysis Of Variance ◽  
Pleurotus Ostreatus ◽  
Oyster Mushroom ◽  
Mycelium Growth ◽  
Alternative Substrates ◽  
Randomized Design ◽  
Mycelium Growth Rate ◽  
Completely Randomized Design

Abstract The study aimed to find alternative substrates for growing spawn of oyster mushroom (Pleurotus ostreatus). The experiment was laid out in a completely randomized design with three compositions of substrates. The substrate compositions were: (1) Substrate A (corn 100%), (2) Substrate B (corn and sawdust mix in a ratio of (1:1, v/v)), and (3) Substrate C (corn and sawdust mix in a ratio of (3:1, v/v)). All compositions were replicated 18 times. The complete colonization (days) and mycelium growth rate (cm day−1) were recorded. Means were analyzed by Analysis of Variance (ANOVA) and duncan test was performed if there were significant between the substrate compositions. The results showed that substrate C was the best substrate indicated with the fastest complete colonization of 14.17±0.92 and the highest growth rate of 0.85±0.06 cm day−1.

scholarly journals Efek Penambahan Limbah Lokal Jerami Dan Sekam Padi Bagi Pertumbuhan Jamur Tiram Putih (Pleurotus ostreatus)

2019 ◽  
Vol 5 (2) ◽  
pp. 70-76
Author(s):  
Afifah Nur Shobah ◽  
Swastika Oktavia
Keyword(s):  
Growth Rate ◽  
Pleurotus Ostreatus ◽  
Dry Weight ◽  
Oyster Mushroom ◽  
Microscopic Fungi ◽  
Artificial Medium ◽  
Mycelium Growth ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

White oyster mushroom (Pleurotus ostreatus) is a group of microscopic fungi that are used as food.  P. ostreatus is cultivated in an artificial medium derived from sawdust and has been sterilized. However, the use of sawdust also has problems. Straw and husk of rice can be used as mushroom growing media because they contain organic ingredients such as cellulose, hemicellulose, and lignin. This study aimed to know the growth of white oyster mushroom (P. ostreatus) on straw and husk of rice as an artificial medium and to know the best composition of straw and husk of rice that can be got highly produced of white oyster mushroom (P. ostreatus). The methods of this research were experimental with ten treatments and included several stages including preparation of tools, materials and research sites, the stage of cultivation of P. ostreatus and data collection. This study used an experimental method with a completely randomized design consisting of 40 experimental units. The results obtained were the most optimal P. ostreatus mycelium growth in K1J2S1 treatment with a mean growth rate of 30,60 cm / 30 days, the highest wet weight was K3J1S0 which was 85,83 g while the highest dry weight was in K1J2S1 treatment that is equal to 8,71 g.

scholarly journals Pengaruh penambahan serbuk jagung pada komposisi media tanam terhadap pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus)

2021 ◽  
Vol 10 (2) ◽  
pp. 158-170
Author(s):  
Muhaeming Muhaeming ◽  
Jamilah Jamilah ◽  
Zulkarnaim Zulkarnaim
Keyword(s):  
Experimental Study ◽  
Analysis Of Variance ◽  
Pleurotus Ostreatus ◽  
Medium Composition ◽  
Oyster Mushroom ◽  
Control Treatment ◽  
Mycelium Growth ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Penambahan nutrisi perlu dilakukan untuk meningkatkan produktivitas pertumbuhan jamur tiram putih (Pleurotus ostreatus). Salah satu bahan yang dapat digunakan adalah serbuk jagung. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan serbuk jagung pada komposisi media tanam terhadap pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus) dan mengetahui konsentrasi serbuk jagung yang paling baik digunakan pada pertumbuhan  miselium bibit F1 jamur tiram putih (Pleurotus ostreatus). Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) 4 perlakuan dan 3 ulangan sehingga diperoleh 12 satuan percobaan; P0 (perlakuan kontrol), P1 (25%), P2 (50%), dan P3 (100%). Variabel yang diukur adalah persentase pertumbuhan miselium yang diamati pada hari ke-6, 12 dan 18 dan waktu penyebaran miselium Hari Setelah Inokulasi (HSI). Data hasil penelitian dianalisis menggunakan analysis of variance (ANOVA) dengan uji lanjut menggunakan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan bahwa ada pengaruh pada pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus) dengan penambahan serbuk jagung pada komposisi media tanam. Selain Itu, konsentrasi serbuk jagung yang memberikan hasil yang paling baik terhadap pertumbuhan miselium adalah P2 dengan konsentrasi 50%. Kata kunci : miselium F1; Pleurotus ostreatus; serbuk jagung  ABSTRACTAdditional nutrition needs to be done to increase the productivity of oyster mushroom (Pleurotus ostreatus) growth. One of the materials that can be used is corn powder. This study aimed to determine the effect of corn powder on the planting medium composition towards mycelium growth of oyster mushroom F1 seeds (Pleurotus ostreatus) and to find the best concentration of corn powder to grow mycelium seeds F1 oyster mushroom (Pleurotus ostreatus). This research was an experimental study using a Completely Randomized Design (CRD) with 4 treatments namely; P0 (control treatment), P1 (25%), P2 (50%), and P3 (100%), and 3 repetitions so that obtained 12 experimental units. The variables measured were the mycelium growth percentage on days 6, 12, and 18, and the mycelium widespread time after the day of inoculation. The data were analyzed using ANOVA then continued on the Least Significant Difference (LSD) test. The results showed that the addition of corn powder on oyster mushroom growing media affected mycelium growth of the oyster mushroom F1 seeds (Pleurotus ostreatus). It also found that the P2 with a 50% concentration of corn powder gave the widest mycelium growth.  Keywords: corn powder; mycelium; Pleurotus ostreatus

scholarly journals Evaluation of Biomass and Chitin Production of Morchella Mushrooms Grown on Starch-Based Substrates

Foods ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 239 ◽  
Author(s):  
Aikaterini Papadaki ◽  
Panagiota Diamantopoulou ◽  
Seraphim Papanikolaou ◽  
Antonios Philippoussis
Keyword(s):  
Growth Rate ◽  
Linear Growth ◽  
Biomass Yield ◽  
Linear Growth Rate ◽  
Waste Streams ◽  
Mycelium Growth ◽  
Potato Dextrose Broth ◽  
Polysaccharide Content ◽  
Potato Peels ◽  
Mycelium Growth Rate

Morchella sp. is one of the most expensive mushrooms with a high nutritional profile. In this study, the polysaccharide content of Morchella species was investigated. Specifically, mycelium growth rate, biomass production, sclerotia formation, and glucosamine and total polysaccharides content of six Morchella species grown on a starch-based media were evaluated. Submerged fermentations in potato dextrose broth resulted in a glucosamine content of around 3.0%. In solid-state fermentations (SSF), using potato dextrose agar, a high linear growth rate (20.6 mm/day) was determined. Increased glucosamine and total polysaccharides content were observed after the formation of sclerotia. Biomass and glucosamine content were correlated, and the equations were used for the indirect estimation of biomass in SSF with agro-industrial starch-based materials. Wheat grains (WG), potato peels (PP), and a mixture of 1:1 of them (WG–PP) were evaluated as substrates. Results showed that the highest growth rate of 9.05 mm/day was determined on WG and the maximum biomass yield (407 mg/g) on WG–PP. The total polysaccharide content reached up to 18.4% of dried biomass in WG–PP. The results of the present study proved encouraging for the efficient bioconversion of potato and other starch-based agro-industrial waste streams to morel biomass and sclerotia eliciting nutritional and bioactive value.

scholarly journals MORPHOLOGICAL RESPONSES OF KING OYSTER MUSHROOM AGAINST AUGMENTATION OF COTTON WASTE WITH CITRUS PEEL

2018 ◽  
Vol 30 (1) ◽  
pp. 99
Author(s):  
Muhammad M. Jahangir ◽  
Muhammad A. Khatana ◽  
Nasir A. Khan ◽  
Ghufrana Samin ◽  
Khurram Ziaf ◽  
...  
Keyword(s):  
Oyster Mushroom ◽  
Biological Efficiency ◽  
Mycelium Growth ◽  
Citrus Peel ◽  
Morphological Attributes ◽  
Mean Values ◽  
Randomized Design ◽  
Cotton Waste ◽  
Growth Initiation ◽  
King Oyster Mushroom

The utilization of agro-industrial waste materials for production of mushrooms has become a crucial research precedence recently. Pleurotus eryngii is considered one of the most famous mushrooms because of nutraceutical and high nutritional value. Therefore, a study was conducted in Medicinal and Mushroom Lab to evaluate the morphological attributes of king oyster mushroom against cotton waste (CW) and citrus peel (CP) based substrates. CW alone and its different combinations with CP i.e. T0: CW (100 %), T1: CW (98%) + CP (2%), T2: CW (96%) + CP (4%), T3: CW (94%) + CP (6%), T4: CW (92%) + CP (8%) and T5: CW (90%) + CP (10%) were used in experiment. The experiment was conducted by applying 2-factor factorial under completely randomized design with 5 replications. Different morphological attributes viz. spawn running and mycelium growth initiation, duration to complete mycelium growth (days), pinhead development, pinheads/bag, duration to complete flushes (1st,2nd, 3rd), mushroom fresh weight, yield/bag and biological efficiency were calculated. Results were analyzed by ANOVA and mean values are equated by LSD test at 5% probability level. Results indicated that spawn running, the growth of mycelium, duration to complete flushes, pinhead development and duration, yield and biological efficiency all were significantly influenced by treatment T2 (CW 96% + CP 4%) as compared to control. Overall results revealed that combinations of citrus peel and cotton waste can be utilized as a promising substrate for king oyster mushroom production.

Pengujian Beberapa Varietas Jamur Tiram Pada Kombinasi Mediaserbuk Ampas Tebu Dan Serbuk Gergajian Dengan Penambahan Molase Dan Limbah Ampas Tahu.

2020 ◽  
Vol 1 (1) ◽  
pp. 26-36
Author(s):  
Ryan Fajar Sidik Siregar ◽  
Erwin Pane ◽  
Siti Mardiana
Keyword(s):  
Data Analysis ◽  
Research Method ◽  
The Body ◽  
Oyster Mushroom ◽  
Media Composition ◽  
Mycelium Growth ◽  
Randomized Design ◽  
Two Factors ◽  
The Media ◽  
High Production

Oyster mushroom is one of the most popular consumed mushrooms and also gives benefit for the body because it contains high nutrition and low fat. The goal of this research was to know and analyse the increase growth of some varieties of oyster mushroom on the bagasse media by adding molasses and tofu dregs. The research method was completely factorial randomized design (RAL Factorial) with two factors. The first factor was the media composition of sawdust and bagasse powder and The second factor was oyster musroom. The data analysis was tested by Anova and continued with duncan test if the results of variance were significantly different to very real. The results showed that the best treatment for mycelium growth on M1 media (100% bagasse + 1% molasses + 6% tofu dregs) with the best combination M2V3 (75% bagasse + 25% sawdust + 1% molasses + tofu dregs 6% on brown oyster mushroom). The largest stem diameter for V1 (white oyster mushroom) was 9.24 cm at the first harvest, and 9.01 cm the second harvest. The best length of the stalk in V3 variety (brown oyster mushroom) was 4.94 cm at the first harvest and 5.11 cm the second harvest. The best treatment on oyster mushroom production showed that variety V1 (white oyster mushroom) showed high production at the first harvest 130.25 grams and the second harvest 132.83 grams.

The Growth Of White Oyster Mushroom Mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) From Liquid And Solid Inoculum

Biocelebes ◽  
2019 ◽  
Vol 13 (3) ◽  
pp. 279-287
Author(s):  
Nisfaun Safitriana ◽  
Umrah ◽  
Orryani Lambui
Keyword(s):  
Liquid Medium ◽  
Incubation Time ◽  
Pleurotus Ostreatus ◽  
Solid Medium ◽  
Oyster Mushroom ◽  
Mycelium Growth ◽  
Average Growth ◽  
Solid Media ◽  
Randomized Design ◽  
Department Faculty

The study about  the growth of white oyster mushroom mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) from liquid and solid inoculum conducted on January until July 2018 in Biotechnology laboratory, Biology Department, Faculty of Math and Science, Tadulako University. This study was aimed to find out the mycelium growth of source of stem inoculum and hood in liquid and solid media. This study was designed by Complete Randomized Design (CRD) which consisted of four treatments and five replications. The treatment were M1 (source of stem inoculum in liquid medium), M2 (source of stem inoculum in solid medium), M3 (source of hood inoculum in liquid medium), and M4 (source of hood inoculum in solid medium). The parameters were (a) incubation time till mycelium fills medium, (b) CFU, and (c) the viability of inoculum on producing medium. The result showed that the faster incubation were M1 and M3 (for 2 days, higher CFU was M3 with an average number 8,2 x 10 10 CFU /ml. The faster viability of mycelium growth were M3 with an average growth of mycelium 6,97 cm/days.

scholarly journals Aggressiveness and trichothecene production of Fusarium graminearum isolates from cereals in Serbia

2021 ◽  
Vol 36 (1) ◽  
pp. 1-13
Author(s):  
Ana Obradovic ◽  
Jelena Vukadinovic ◽  
Milica Nikolic ◽  
Aleksandra Bulajic ◽  
Goran Stankovic ◽  
...  
Keyword(s):  
Growth Rate ◽  
Statistical Analysis ◽  
Fusarium Graminearum ◽  
Statistical Correlation ◽  
Hplc Method ◽  
Mycelium Growth ◽  
Barley Leaf ◽  
Mycelium Growth Rate

The aim of this study was to assess variations in aggressiveness and trichothecene production of F. graminearum isolates originating from maize, wheat and barley in Serbia. Analyzing F. graminearum isolates (98) obtained from various agroecological conditions of Serbia over the period from 1993 to 2010, using the HPLC method, the following two chemotypes were observed: 3-acetyl-deoxinivalenol (3ADON) and 15-acetyl-deoxinivalenol (15ADON). A great diversity in the production of deoxinivalenol (DON) derivatives was observed. A majority of F. graminearum isolates, regardless of their origin (maize, wheat or barley) belonged to the 15ADON chemotype. The 3ADON chemotype was also detected, but in a significantly smaller number (13/98) samples, compared to the 15ADON chemotype (85/98). None of the tested isolates belonged to the NIV chemotype. The examined isolates showed different pathogenicity on barley leaf, wheat class and maize ears. The average pathogenicity of the tested isolates was the highest on barley leaf. It was observed that isolates originating from wheat had the highest average daily increase in mycelium growth rate (27.37 mm). Statistical analysis of the obtained results for mycotoxins synthesis showed that there was a highly significant statistical correlation between the production potentials of total DON, 3ADON and 15ADON in F. graminearum isolates belonging to various chemotypes. However, there was no statistically significant correlation between the aggressiveness of isolates and the production of total DON in isolates belonging to 3ADON and 15ADON chemotypes.

scholarly journals Optimization of growth of oyster mushroom mycelium (Pleurotus sp.) from Tasikmalaya on several kinds of cereal medium

2019 ◽  
Vol 1 (2) ◽  
pp. 13-17
Author(s):  
Khusnul Khusnul
Keyword(s):  
Human Body ◽  
Edible Mushroom ◽  
Oyster Mushroom ◽  
Mushroom Cultivation ◽  
Mycelium Growth ◽  
Randomized Design ◽  
Growing Medium ◽  
Completely Randomized Design ◽  
Seed Medium

Pleurotus sp. or oyster mushroom is one type of edible mushroom that contains essential nutrients for the human body. This mushroom has been widely cultivated in Indonesia, one of which is Pleurotus sp. from Tasikmalaya. The key to the success of oyster mushroom cultivation is the seeds and growing medium or baglog, while the factors that influence the quality of the seeds include the type of medium. This study aims to determine 1) the effect of the type of seed medium from the type of cereals used on the growth of mycelium Pleurotus sp. from Tasikmalaya, 2) determine the type of seed medium that has the best mycelium growth. This study uses an experimental method with a Completely Randomized Design (CRD). The parameters measured include the main parameters, namely the rate of growth of mycelium Pleurotus sp. from Tasikmalaya on the seed medium. Supporting parameters are the quality of mycelium growth in the seed medium, the proximate value of cereals used for the seed medium. The results of the analysis showed that the medium of cereals used as seeds ready for cultivation could affect the quality of the seeds produced. The seedling medium from milled corn has the best growth of mycelium.

scholarly journals Synthesis of Luminescent Ag Nanoclusters with Antibacterial Activity

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Ying-Fan Liu ◽  
Lei Wang ◽  
Chun-Po Bu ◽  
Guo-Qing Wang ◽  
Yan-Hui Zhang ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Growth Rate ◽  
Antibacterial Activity ◽  
Blue Emission ◽  
Antimicrobial Effect ◽  
Mycelium Growth ◽  
Mycelium Growth Rate ◽  
Strong Blue Emission ◽  
Ag Nanoclusters ◽  
Ag Ncs

This paper focuses on the synthesis of novel Ag nanoclusters (NCs) using DHLA as capping reagents in aqueous solution by a photoreduction method. Luminescence studies indicated that the DHLA-Ag NCs exhibited strong blue emission with maximum peak at 480 nm. The maximum emission of the NCs can be greatly improved with irradiating time by around 15-fold from 3 h to 67 h. By means of mycelium growth rate, the results showed that the Ag NCs with smaller sizes had a good antimicrobial effect.

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