scholarly journals More about the biodiversity of parasites in Vietnam: a first report of microsporidia (opistosporidia, microsporidia) in coral fish

2021 ◽  
Vol 937 (2) ◽  
pp. 022067
Author(s):  
H Vo ◽  
F Butaeva ◽  
V Yurakhno

Abstract This is the first report of microsporidia infection in marine fish from Vietnam. Microsporidia (Opistosporidia, Microsporidia) were identified in 4 species of coral fish Cheilinus chlorourus (Labridae), Abudefduf bengalensis (Pomacentridae), Pomacentrus brachialis (Pomacentridae), Atule mate (Carangidae) from Nha Trang Bay (East Sea), Khanh Hoa Province, Vietnam, in 2018 and 2019. Dry smears of blood, intestine and liver were stained using the Giemsa method. Gut and liver were fixed in Bouin’s fluid, processed for routine paraffin infiltration, and sections were stained with Hematoxylin and Eosin. Strong microsporidia infections were the same for all three species revealed in 1 of every 6 fish samples (prevalence 16.7%) of C. chlorourus, A. bengalensis, and P. brachialis. In C. chlorourus there were meronts, sporogonial plasmodia, and sporoblasts found in all layers of intestine (enterocytes, myocytes, mesotheliocytes), pancreas and liver. In A. bengalensis parasites were found in enterocytes of the small intestine and liver. In P. brachialis meronts, sporogonial plasmodia and sporoblasts were in enterocytes of the small intestine. Intracellular parasites are located in parasitophorous vacuoles covered with additional shell, which appeared to be in the process of forming xenoma. Microsporidia infection was revealed in 1 of 31 fish samples of A. mate (prevalence 3 %). Up to 30 of round and oval white xenomas were detected in the gills of fresh samples by microscopy. We tentatively assign the microsporidia to Glugeidae due to host affiliation (fish), localization (digestive system) and xenoma formation. They differ from Pleistophoridae by localization in non-muscular cells.

2021 ◽  
Vol 32 (2) ◽  
pp. 303-312
Author(s):  
MD. BOKTHIER RAHMAN ◽  
MD. SAZEDUL HOQUE ◽  
SUPRAKASH CHAKMA ◽  
SHAIDA AKTER ◽  
S.M. OASIQUL AZAD ◽  
...  

The study was conducted in aims to investigate the effects of frozen storage and cooking conditionson proximate compositions and formaldehyde content (FA) in some selected fish from three different sourcesin Bangladesh. Proximate composition in fresh and final frozen samples was determined by standard AOACmethod and FA content in fresh, frozen stored, and cooked samples was determined by spectrophotometricmethod. Among the studied fishes, marine fish contained higher protein (except Rita), lipid, and ash followedby estuarine and culture fish samples. Protein, moisture and ash content decreased and lipid content increasedsignificantly (p<0.05) during frozen storage for all samples and sources. The FA was lower in cultured fishsamples compared to that of the river and marine fish samples, both at fresh and end of frozen storage. Atfresh condition, FA content in all samples ranged from 0.41 to 0.71µg/g, 0.51 to 0.89µg/g, and 0.73 to1.69µg/g which increased to 0.95 to 2.11µg/g, 1.74 to 1.95µg/g, and 3.22 to 5.20µg/g at end of the storageperiod, respectively (p<0.05). Further, FA content significantly decreased after cooking in all the fishsamples (p<0.05). However, irrespective of fish species and sources, the FA content was higher than WHOrecommended value (0.2 µg/g). The study findings revealed that longer frozen storage of fish could be apublic health concern to the consumers.


2018 ◽  
Vol 8 (2) ◽  
Author(s):  
Md. Mazharul Islam ◽  
Robiul Islam ◽  
Mohammad Shoeb ◽  
Nilufar Nahar

Author(s):  
D. Bangieva ◽  
D. Stratev ◽  
T. Stoyanchev

Background: Histamine is an essential biogenic amine produced as a result of microbial decomposition of histidine during seafood processing and storage. The objective of this study was to evaluate histamine concentration in freshwater and marine fish marketed in Stara Zagora region, Bulgaria. Methods: Forty fish samples were purchased from local fish farms and retail stores in Stara Zagora, Bulgaria. Enzyme-Linked Immunosorbent Assay was used to determine histamine levels. The data were processed using GraphPad Software InStat 3. Results: Histamine was detected in 26 out of 40 (65%) samples, and none of them exceeded the regulatory limit of 200 mg/kg. The average histamine content in marine fish (6.965±3.187 mg/kg) was insignificantly (p>0.05) higher than that in freshwater fish (4.503±1.133 mg/kg). Conclusion: The results reveal low levels of histamine in freshwater and marine fish indicating their good quality. However, its presence in seafoods remains a major food safety problem that requires permanent regulation of histamine concentration in fish.  


2019 ◽  
Vol 49 (2) ◽  
pp. 162-165
Author(s):  
Lisiane Lappe dos REIS ◽  
Lucas Carvalho de JESUS ◽  
Ormezinda Celeste Christo FERNANDES ◽  
David Eduardo BARROSO

ABSTRACT Most species of Myxobolus (Cnidaria: Myxozoa) infect freshwater and marine fish, and cause sporozoosis. Passage of myxozoan parasites into human feces has been described occasionally. We examined fecal samples from 97 dwellers of a riverine community in Amazonas State (Brazil), which revealed Myxobolus sp. in 13 (13%) samples. The discovery probably does not represent true parasitism as the myxospores - most likely ingested through the consumption of infected fish - were eliminated unchanged after passing through the gastrointestinal tract. This discovery represents the first report of this myxosporean in human fecal samples in Brazil.


1982 ◽  
Vol 19 (7_suppl) ◽  
pp. 26-32 ◽  
Author(s):  
J. Zeller ◽  
A. Takeuchi

Intestinal spirochetosis, an infection of the mucosa by spiral-shaped organisms, was studied in clinically normal rhesus monkeys (Macaca mulatta) by histology, transmission and scanning electron microscopy. The incidence of intestinal spirochetosis was 42% in 221 monkeys. Spiral organisms stained with hematoxylin and eosin (HE) appeared as a broad basophilic haze on the colonic surface and were strongly positive by the Warthin-Starry stain. Spiral-shaped bacteria include two structurally different organisms: spirochetes and flagellated microbes. They intimately populated the brush border of the surface of the epithelium of the large intestine. They were absent in the crypts and in the small intestine. Infection by spirochetes produced no alteration of cytocomponents of the underlying host structures. Spirochetes and flagellates infrequently penetrated beyond the brush border into the epithelial cytoplasm and also into the lamina propria. Even in cases where invasion was documented, no inflammatory response was found.


Author(s):  
Faruk Abrão KALIL-FILHO ◽  
José Simão de Paula PINTO ◽  
Emerson P BORSATO ◽  
Carlos Henrique KURETZKI ◽  
Bruno Luiz ARIEDE ◽  
...  

Abstract Background: The creation of a computerized clinical database with the ability to collect prospective information from patients and with the possibility of rescue and crossing data enables scientific studies of higher quality and credibility in less time. Aim: To validate, in a single master protocol, the clinical data referring to Surgery of Digestive System in a multidisciplinary way, incorporating in the SINPE© platform, and to verify the incidence of digestive diseases based on the prospectively performed collections. Method: Organize in one software, in a standardized structure, all the pre-existing items in the SINPE© database; the theoretical basis was computerized through the MIGRASINPE© module creating a single multiprofessional master protocol for use as a whole. Results: The existing specific protocols were created and/or adapted - they correspond to the most prevalent digestive diseases - unifying them. The possibility of multiprofessional use was created by integrating all data collected from medicine, nursing, physiotherapy, nutrition and health management in a prospective way. The total was 4,281 collections, distributed as follows: extrahepatic biliary tract, n=1,786; esophagus, n=1015; anorectal, n=736; colon, n=550; small intestine, n=86; pancreas, n=71; stomach, n=23; liver, n=14. Conclusions: The validation of the unification and structuring in a single master protocol of the clinical data referring to the Surgery of the Digestive System in a multiprofessional and prospective way was possible and the epidemiological study carried out allowed to identify the most prevalent digestive diseases.


2020 ◽  
Vol 3 (2) ◽  
pp. 86
Author(s):  
Luh Gde Surya Heryani ◽  
Ni Nyoman Werdi Susari ◽  
Luh Putu Syamadina Pramesya Nareswari

Information on the anatomical and morphometric structure of the digestive system of Bali cattle will provide a clear and precise picture if abnormalities occur in the organs in the digestive system. This study aims to determine the anatomical and morphometric structures of the small intestine and large intestine of Bali cattle. Samples were taken from 14 Bali cattle and based on gross pathology examination declared healthy. The results showed that the average length of the small intestine including the duodenum, jejunum and ileum are 473.07 cm, 529.64 cm, and 363.64 cm; and successive widths are 5.50 cm, 6.00 cm, and 6.11 cm. While the average length of the large intestine which includes the cecum, colon and rectum are 36.78 cm, 371.21, 50.00 cm; while the width are 9.65 cm, 11.47 cm, and 8.85 cm. Jejunum has the longest size in the small intestine, while in the large intestine the longest size is the colon. The right information and data about the digestive system are very important and useful to support further clinical and preclinical research, and the results of this study can be used as a reference in determining the characterization of Bali cattle.


2017 ◽  
Vol 9 (11) ◽  
pp. 10980
Author(s):  
Sanku Borkataki ◽  
Pankaj Goswami ◽  
Rajesh Katoch ◽  
Shahil Kumar ◽  
Pratiksha Raghuvanshi

The treamatode Artyfechinostomum sufrartyfex was identified from the small intestine of the Indian Mongoose and the morphological identification of the parasites was described.  The fluke A. sufrartyfex is important considering its significant zoonotic potential, and the present study highlights the first report of occurrence of the parasite in a newer wild host, the mongoose from India. 


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