scholarly journals Choice of explant, its size and method of its placement in a test tube to increase the productivity of clonal micropropagation of Fioletoviy ranniy variety

2021 ◽  
Vol 937 (2) ◽  
pp. 022114
Author(s):  
V Puzirnova ◽  
N Doroshenko
Keyword(s):  
Survival Rate ◽  
Test Tube ◽  
Growth Processes ◽  
Micro Cutting ◽  
Grapevine Variety ◽  
Clonal Micropropagation ◽  
Factors Influencing

Abstract The paper is devoted to the optimization of one of the factors influencing the success of micropropagation – the choice of the optimal part of vine to take explant, size of the explant and the method of its exposure in a test tube. The studies have confirmed that the place, where micro-cutting was taken, its size and its orientation during planting can affect the speed of growth processes and the survival rate of plants. The experiment was put on the Purple Early grapevine variety. The part of vine for taking micro-cutting, best size of explant and the method of its orientation in the test tube are established.

scholarly journals REGENERATION OF MICRO CUTTINS WITH DIFFERENT SIZE AND ORIENTATION IN TEST TUBE

Russian vine ◽  
2021 ◽  
Vol 18 ◽  
pp. 23-27
Author(s):  
V.G. Puzirnova ◽  
◽  
N.P. Doroshenko ◽  
Keyword(s):  
Survival Rate ◽  
Test Tube ◽  
Optimal Size ◽  
Grape Variety ◽  
Growth Processes ◽  
Factors Influencing

The article is devoted to the optimization of one of the factors influencing the success of micropropagation – the choice of the optimal size of the explant and the method of its expo-sure in a test tube. Many studies have con-firmed that the size of the micro cuttings and its orientation during planting can affect the speed of growth processes and the survival rate of plants. The experiment was set on the Fio-letoviy Ranniy grape variety. The best size of the micro cuttings and the method of its orien-tation in the test tube are established.

Incidence of Uterine Torsion and Related Factors in Buffaloes of Amul Milk Shed Area

2019 ◽  
Vol 15 (01) ◽  
pp. 17-20
Author(s):  
Chintan S Pateliya1 ◽  
J A Patel ◽  
A J Dhami ◽  
S B Patel ◽  
H L Makwana
Keyword(s):  
Retrospective Study ◽  
Survival Rate ◽  
Caesarean Section ◽  
Post Treatment ◽  
Full Term ◽  
Related Factors ◽  
Factors Influencing ◽  
Uterine Torsion

A study was carried out to find out the retrospective incidence of uterine torsion in buffaloes among obstetrical cases in Amul milk shed area and factors influencing it. Data on 1,13,772 obstetrical cases attended from January 2017 to June 2018 by Amul Veterinarians were collected. Moreover, 50 buffaloes suffering from uterine torsion were taken up during July-December, 2018 to know the side, site, degree of uterine torsion, parity as well as sex and viability of the calf and the dam after detorsion/Caesarean section in the same area. In the retrospective study, a total of 2000 cases of uterine torsion were recorded among total 1,13,772 bovine obstetrical cases, which encompassed 1.76%. Of the 2000 cases, 92.20 (1844) percent torsions were found in buffaloes only. Among 57,111 obstetrical cases attended in buffaloes, the incidence of uterine torsion was 3.23%. Moreover, the region/center-wise incidence of torsion cases varied from 1.30–19.36%. The highest incidence of uterine torsion was found in Anand region (19.36 %) followed by Kathlal (11.23 %) and Mahemdabad (10.14 %) regions, while the lowest incidence was in Virpur (2.06%), Petlad (1.46%) and Piplata (1.30%). It was concluded that buffaloes mostly experience right side (100%), post-cervical (82%) uterine torsion of 270–360° (66%), at full term of gestation (70%)with lower survivability of the calves (30%), however, the survival rate of the dams post-treatment was 90%.

Factors Possibly Influencing the Prognosis of Oligodendroglioma

Neurosurgery ◽  
1988 ◽  
Vol 22 (5) ◽  
pp. 886-891 ◽  
Author(s):  
Zhen Min Sun ◽  
Shigeru Genka ◽  
Nobuyuki Shitara ◽  
Atsuo Akanuma ◽  
Kintomo Takakura
Keyword(s):  
Radiation Therapy ◽  
Survival Rate ◽  
Extent Of Resection ◽  
Blood Groups ◽  
Survival Period ◽  
Postoperative Survival ◽  
Tumor Characteristics ◽  
Abo Blood Groups ◽  
Factors Influencing ◽  
The University

Abstract Fifty-seven cases of oligodendroglioma (including eight cases of malignant oligodendroglioma) treated at the University of Tokyo Hospital between 1961 and 1985 were analyzed for factors influencing the survival rate. Factors related to a poor outcome were findings of malignancy and symptoms of dementia. Survival rate and postoperative survival period were not influenced significantly by radiation therapy, extent of resection, tumor characteristics, or ABO blood groups.

A review of the factors influencing the prognosis of medulloblastoma

1987 ◽  
Vol 66 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Anthony J. Caputy ◽  
David C. McCullough ◽  
Herbert J. Manz ◽  
Kathleen Patterson ◽  
Mary Kathryn Hammock
Keyword(s):  
Cell Differentiation ◽  
Survival Rate ◽  
Histological Finding ◽  
Tumor Stage ◽  
Multivariate Approach ◽  
Content Type ◽  
Factors Influencing ◽  
Significant Difference ◽  
Extent Of Surgical Resection ◽  
Fine Print

✓ The clinical, therapeutic, and histological features of 54 patients with medulloblastoma were analyzed retrospectively by a multivariate approach with regard to prognosis. The overall 5-year survival rate was 60%, with 48% of patients free of recurrence at 5 years. Cell differentiation, when present, was associated with a significantly longer recurrence-free period. Seventy-two percent of patients with the histological finding of cell differentiation were recurrence-free at 5 years. A marginally significant increase in the 5-year survival rate was also seen in association with differentiation. Only 34% of the patients whose tumor exhibited necrosis were alive at 5 years. There was no statistically significant difference in 5-year survival for children under 3 years of age or for the group of children aged 5 years or under. However, a significantly larger fraction (72%) of the group aged 5 years or under had a recurrence-free period of 5 years or more. Other factors including sex, extent of surgical resection, Chang tumor stage, posterior fossa radiation dose, and adjuvant chemotherapy did not influence prognosis.

Factors influencing development of free-living generations of Strongyloides stercoralis

Parasitology ◽  
1988 ◽  
Vol 97 (1) ◽  
pp. 129-138 ◽  
Author(s):  
K. Shiwaku ◽  
Y. Chigusa ◽  
T. Kadosaka ◽  
K. Kaneko
Keyword(s):  
High Temperature ◽  
Incubation Temperature ◽  
Strongyloides Stercoralis ◽  
Test Tube ◽  
Paper Test ◽  
Free Living ◽  
Factors Influencing ◽  
Prednisolone Treatment ◽  
Duration Of Infection ◽  
Incubation Temperatures

SUMMARYThe effects of duration of infection and of temperature and nourishment in cultures on development of free-living generations of Strongyloides stercoralis were studied quantitatively. Rhabditiform larvae, 228–353 μm long, were collected from infected dogs with or without prednisolone treatment using the Baermann apparatus. Cultures were carried out by the filter paper test-tube method under the following conditions: incubation temperature 15–40°C and faecal dilution 1:0–1:16. Rhabditiform larvae developed predominantly to free-living females at incubation temperatures of 15–30°C and low faecal dilutions, but filariform larvae appeared mainly under extreme conditions such as high temperature. Recovery rates of filariform larvae were inversely related to those of females. It was remarkable that high temperature, but not low faecal dilution, affected development of filariform larvae. Although the appearance of free-living males was constant in various environmental conditions, the present study indicated an increase in free-living males with the duration of infection. Thus, it seems that free-living males are already fixed as male in the egg stage, and potential female rhabditiform larvae differentiate into free-living females or filariform larvae depending on environmental factors. There is no marked difference in the development of rhabditiform larvae into filariform larvae in either the immunosuppressed dog or the intact dog.

scholarly journals Clonal Micropropagation of Cranberry (Oxycoccus palustris Pers.)

2021 ◽  
Vol 51 (1) ◽  
pp. 67-76
Author(s):  
Sergey Makarov ◽  
Irina Kuznetsova ◽  
Mikhail Upadyshev ◽  
Sergey Rodin ◽  
Anton Chudetsky
Keyword(s):  
Survival Rate ◽  
Nutrient Medium ◽  
Average Length ◽  
Forest Products ◽  
Hybrid Form ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Total Growth

Introduction. The last decade saw a considerable increase in the demand for European cranberry planting material (Oxyccocus palustris Pers.) among consumers of non-timber forest products. Cranberry possesses high nutritional and medicinal value. Cultivars and hybrids of European cranberry prove extremely productive for plantation growth using the method of clonal micropropagation with revitalized planting material. Study objects and methods. The research featured European cranberry plants of the Dar Kostromy cultivar and its hybrid form 1-15-635. The study focused on the effect of various medications and growth regulators on the biometric profile of European cranberry and its adaptation to non-sterile conditions at all stages of in vivo clonal micropropagation. Results and discussion. During the introduction stage, the highest viability belonged to the explants treated with AgNO3 (95–96%) and Lizoformin 3000 (5%) as the main sterilizing solutions at a 10-min exposure and a 5% solution of Ecosterilizer (1:1) at a 20-min exposure (90–95%). During the micropropagation proper, the number, average length, and total growth of shoots increased as the concentration of cytokinin 2ip in the WPM 1/4 nutrient medium rose from 1.0 to 5.0 mg/L. At the stage of in vitro rooting, the maximal number, average length, and total growth of roots in regenerated plants for both cultivars were observed when Kornerost 5.0 mg/L was added to the WPM 1/4 nutrient medium. At the stage of adaptation to in vivo conditions, Micogel 0.2 mg/L contributed to the highest survival rate (94–100%). Conclusion. During clonal micropropagation in vitro, the biometric profile of European cranberry (Oxyccocus palustris Pers.) and its survival rate under non-sterile conditions in vivo proved to depend on various growth-regulating substances and their concentrations.

scholarly journals Clonal micropropagation of decorative cereal Molinia caerulea (L.) Moench

2020 ◽  
Vol 21 (6) ◽  
pp. 713-720
Author(s):  
T. G. Lekontseva ◽  
A. V. Fedorov
Keyword(s):  
Acetic Acid ◽  
Survival Rate ◽  
Shoot Length ◽  
Ms Medium ◽  
Molinia Caerulea ◽  
Clonal Micropropagation ◽  
Micro Propagation ◽  
Shoot Size ◽  
Free Media ◽  
Indole 3 Acetic Acid

The research is devoted to the development of clonal micro-propagation technology of the decorative cereal Molinia caerulea (L.) Moench. The concentration of 2.0 mg /l of cytokinin 6-benzylaminopurine (6-BAP) contributed to obtaining the maximum number of microshoots: 6.3 and 7.9 pcs. on Anderson's and Murashige-Skoog's (MS) media, respectively, which exceeded the control (by 4.7 and 6.3, respectively, with LSD05 = 2.3). The length shoots were observed on hormone-free media, this indicator significantly decreased with an increase in 6-BAP content. On Anderson and MS media with 1.0 mg/l 6-BAP, the shoot length averaged 21.5 and 26.4 mm, respectively, which made it possible to transplant them for rooting, bypassing planting on a medium for elongation. The inclusion of the Siliplant micro-fertilizer in the MS medium at doses of 1.0 and 2.0 ml/l contributed to a significant increase in shoot size, by 16.7 and 10.7 mm (LSD05 = 8.9), respectively, in comparison with the control (MS). It is recommended to use Anderson's medium and 0.5 mg/l of indole-3-acetic acid as a medium for rhizogenesis: after two weeks of cultivation, the regenerants had a standard appearance with developed roots suitable for planting for adaptation. At the adaptation stage, watering the substrate with the biofungicide «Trichoderma veride» according to the instructions and a cereals single spraying with the micro-fertilizer «Siliplant» at a dose of 1.5 ml/l contributed to their 100 % survival rate.

scholarly journals USE OF SALICYLIC ACID IN THE PRODUCTION OF GRAFTED GRAPEVINE SEEDLINGS

Russian vine ◽  
2021 ◽  
Vol 15 ◽  
pp. 30-35
Author(s):  
N.G. Pavluchenko ◽  
◽  
S.I. Melnikova ◽  
O.I. Kolesnikova ◽  
N.I. Zimina ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Root System ◽  
Root Formation ◽  
Cabernet Sauvignon ◽  
Tissue Formation ◽  
Growth Processes ◽  
Grapevine Variety ◽  
Nursery Production ◽  
Root Stock ◽  
Wound Tissue

The aim of the study was to evaluate the ef-fect of salicylic acid on the processes of re-generation and root formation in vine grafts during the stratification period. The efficiency of treatment of copulation sections on root-stock cuttings of vines with Salicylic acid (SC) solution was studied) (10-2, 10-4, 10-6, 10-8) during the production of grafted grapevine seedlings. For setting up the experiment, we used the grafted grapevine variety Cabernet Sauvignon, the rootstock - Kober 5BB. In some variants of the experiment, a reduction in the period of vaccination stratification was noted, due to the activation of the process of wound tissue formation. At the same time, the use of salicylic acid stimulated the budding of the scion, the growth of shoots and the devel-opment of the root system. With an increase in the concentration of the solution to 10-2, a slowdown in the growth of shoots was noted. The present results indicate that salicylic acid, being an endogenous stimulator of growth processes, can be used in nursery production.

scholarly journals INFLUENCE OF GRAPHENE OXIDE ON MICROCLONES OF DOWNY BIRCH AT THE ADAPTATION STAGE

2021 ◽  
Vol 11 (2) ◽  
pp. 48-58
Author(s):  
Nataliya Strekalova ◽  
Olga Zakharova ◽  
Petr Baranchikov ◽  
Alexander Gusev
Keyword(s):  
Graphene Oxide ◽  
Survival Rate ◽  
Beneficial Effect ◽  
Carbon Nanomaterials ◽  
Root Zone ◽  
Control Group ◽  
Point Scale ◽  
Biological Impact ◽  
Clonal Micropropagation ◽  
Downy Birch

In this work, we studied the effect of graphene oxide on regenerants of downy birch when transferred to non-sterile greenhouse conditions (adaptation stage) during clonal micropropagation. During the experiment, it was found that a solution of graphene oxide at a concentration of 1.5 μg / l had a beneficial effect on the development of leaves and plant adaptability (+ 8%). However, with an increase in the concentration of nanomaterial to 3 μg / L, the survival rate decreased by 10% , the plant height - by 25%, and the number of adapted plants - by 35% relative to the control group. In addition, general condition of the seedlings of this group was assessed only by 3 points on a 5-point scale. The study of the content of graphene oxide in plant organs did not reveal pronounced foci of accumulation, which may indicate an indirect effect of nanomaterial on plants through changes in environmental conditions, for example, the availability of root nutrition or the composition of microbiota in the root zone. The mechanisms of ecological and biological impact of carbon nanomaterials on plants are not fully known and require further in-depth research

scholarly journals THE DEPENDENCE OF GROWTH PROCESSES OF THE SCOTS PINE IN THE INITIAL PERIODS OF ONTOGENESIS ON THE ACTION OF IRON NANOPARTICLES

2020 ◽  
Author(s):  
Т.В. БУРДУЧКИНА ◽  
Г.Н. ФАДЬКИН ◽  
С.Д. ПОЛИЩУК
Keyword(s):  
Survival Rate ◽  
Scots Pine ◽  
Iron Nanoparticles ◽  
Aqueous Suspension ◽  
Nitrogen And Phosphorus ◽  
Growth Processes ◽  
Average Growth ◽  
Trunk Diameter ◽  
Pine Seedlings ◽  
Phosphorus And Potassium

В статье приведен анализ влияния нанопорошка железа на приживаемость и сохранность растений, а также на ростовые процессы сосны обыкновенной в начальные периоды онтогенеза. Эффективность препарата оценивали по степени приживаемости и сохранности, а также по текущему и среднему приросту в высоту и диаметре ствола в зоне корневой шейки и химическому составу однолетней хвои. Исследования проводились на дерново-подзолистой почве (ТЛУ А2) в трех лесничествах Рязанской области. В качестве контрольного варианта использовалась общепринятая технология создания лесных культур сосны обыкновенной. Исследуемый вариант предполагал замачивание корневой системы растений сеянцев сосны обыкновенной в водной суспензии нанопорошка железа. Было установлено, что наилучший эффект от применения нанопорошка железа можно ожидать в экстремальные по погодным условиям годы. Так в 2010 году увеличение приживаемости составило 8 %, в 2013 году – 28 %, а в 2016 году 3 %, увеличение текущего прироста в высоту при использовании нанопорошка железа составило 0,41 см в опыте 1 и 1,63 см в опыте 3. В опыте 2 увеличение среднего прироста в высоту было наибольшим и составило 15,35 см. Текущий и средний приросты в диаметре ствола в зоне корневой шейки во всех трех опытах не имели существенных различий. Содержание азота, фосфора и калия в однолетней хвое без применения нанопорошка железа соответствовало древесной породе и ее возрасту. Использование нанопорошка железа во всех трех опытах увеличило содержание азота и фосфора в хвое и не изменило содержания калия. The article presents an analysis of the effect of iron nanoparticles on the survival rate and safety of plants, as well as on the growth processes of the Scots pine in the initial periods of ontogenesis. The effectiveness of the preparation was evaluated by the degree of survival and safety, as well as by the current and average growth in height and trunk diameter in the zone of the root neck and the chemical composition of the annual needle. The studies were conducted on sod-podzolic soil (TLU A2) in three forestries of Ryazan region. As a control option, the generally accepted technology for creating common pine forest plantations was used. The investigated option suggested the soaking of the root system of the Scots pine seedlings in an aqueous suspension of iron nanoparticles. It was found that the best effect of the use of iron nanoparticles can be expected in extreme weather years. So in 2010, the increase in survival rate was 8%, in 2013 it was 28%, and in 2016 it amounted to 3%. The increase in current growth in height using iron nanoparticles was 0.41 cm in experiment 1 and 1.63 cm in experiment 3. The increase in average growth in height was the largest in experiment 2 and amounted to 15.35 cm. The current and average increments in the trunk diameter in the zone of the root neck in all three experiments did not have signifi cant differences and was comparable to the years of research. The content of nitrogen, phosphorus and potassium in the annual needle without the use of iron nanoparticles corresponded to the tree species and its age. The use of iron nanoparticles in all three experiments increased the nitrogen and phosphorus content in the needle and did not change the potassium content.

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