scholarly journals A Potent Staphylococcus Aureus Growth Inhibitor Of A Dried Flower Extract Of Pinus Merkusii Jungh & De Vriese And Copper Nanoparticle

Author(s):  
Masruri Masruri ◽  
Dinna Norani Pangestin ◽  
Siti Mariyah Ulfa ◽  
Slamet Riyanto ◽  
Arie Srihardyastutie ◽  
...  
1969 ◽  
Vol 15 (6) ◽  
pp. 501-507 ◽  
Author(s):  
M. A. Stillwell ◽  
F. A. Wood ◽  
G. M. Strunz

A growth inhibitor with the molecular formula C10H10O4Cl2 was isolated from a medium which had supported growth of a species of Cryptosporiopsis, an imperfect fungus. The compound was named cryptosporiopsin and was characterized on the basis of its physical and chemical properties. Cryptosporiopsin was active in vitro against many wood-rotting basidiomycetes as well as some phycomycetes, ascomycetes, and deuteromycetes. It was more effective than Dithane M-22 or nystatin against the in vitro spore germination of Phytophthora infestans. It was active to varying degrees against six species of bacteria. Cryptosporiopsin was compared with Orbenin and streptomycin against a penicillin-resistant strain of Staphylococcus aureus.


2020 ◽  
Vol 3 (1) ◽  
pp. 25-28
Author(s):  
Sundari Sundari

Along with the needs of quality preparations, of course the ingredients needed must be good, except  the coloring preparations. Dyes that are widely used in practical activities are synthetic dyes.  However, natural dyes from plants are preferred due to the efficiency in their use while working with the same function. Natural dyes are safer to use even though the degree of stability to heat, light and acidity is uncertain. The purpose of this study was to determine if rosella flower extract (Hibiscus sabdariffa) could be used as a substitute for fuchsin in Gram staining. This research was conducted by the experiments with laboratory scale using in vitro method. The results of the study concluded that rosella flower extract could not be used as natural dye for bacterial staining particularly for Staphylococcus aureus and E.coli bacterial preparations.


2021 ◽  
pp. 151-159
Author(s):  
Sari Suryanah ◽  
Syifa Nurjannah

This study aimed to determine the effectiveness of antibacterial formulations derived from herbal plants against mastitis-causing bacteria in dairy cattle. Herbal extracts composed of betel leaves extract, kecombrang flower extract, and turmeric extract were tested against Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli. The research was carried out in a Completely Randomized Design (CRD) using ten treatments and four replications at the Laboratory of Research and Testing, Faculty of Animal Husbandry, Padjadjaran University. The results showed that herbal antibacterial formulations could inhibit the growth of Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteria. The most inhibitory power was obtained from P8 treatment (50% betel leaves + 50% kecombrang flower + 50% turmeric) categorized in "strong inhibition." The percentage of antibacterial inhibition of P8 formulation compared to chloramphenicol was 32% against Staphylococcus aureus, 33% against Streptococcus agalactiae, and 31% against Escherichia coli. Conclusively, the best herbal antibacterial formulation was a combination of 50% betel leaves extract, 50% kecombrang flower extract, and 50% turmeric extract.


Author(s):  
Mardho Tillah ◽  
Irmanida Batubara ◽  
Rita Kartika Sari

<p class="Abstract">The most common human pathogen that colonizes in a third of healthy people around the world are <em>Staphylococcus aureus</em>, and one of the materials allegedly able to overcome the pathogen is resin. Resin has been used in folk medicine for thousands of years to treat diseases. The antimicrobial activity of natural resins can be associated with a variety of organic compounds contained in them such as diterpenoids and triterpenoids. This research aimed to explore the antibacterial and antioxidant activities of <em>Pinus merkusii, P. oocarpa, P. insularis, Agathis loranthifolia</em> resins and essential oil. Resin was separated by distillation process to get essential oil and the residue was extracted using <em>n</em>-hexane, ethyl acetate (EtOAc), and methanol (MeOH). Antioxidant activity was performed by DPPH (1,1diphenyl-2-picryl hydrazyl) radical scavenging method. The antibacterial activity of resins and essential oil of the samples determined using the disc diffusion method against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>. The results showed that the yield of resin extract was ranging from 8.44 % to 95.56%. All extracts and essential oil could not inhibit <em>E coli </em>growth but inhibit the <em>S. aureus </em>growth. This experiment concluded that resin <em>n</em>-hexane extract from <em>P.oocarpa</em> was the most potent as antibacterial activity against <em>S. aureus</em>. All of the samples used had less potential antioxidant activity compared to positive control ascorbic acid. Result of this study show that pine resin from Indonesia has potential as an antibacterial agent. </p>


2017 ◽  
Vol 13 (2) ◽  
pp. 239
Author(s):  
Wahyu Haryati Maser ◽  
Nancy Dewi Yuliana

Kecombrang (<em>Etlingera elatior</em>) is a well-known herb in Indonesia that is widely used in traditional medicine and as a flavour in local dishes. The aim of this study was to identify retention time of antibacterial components from kecombrang flower extract (<em>Etlingera elatior</em>) by HPLC-based metabolomics method. The antibacterial activity of ethanol extracts, chloroform, and water fractions was assessed by well-diffusion agar method against <em>Staphylococcus aureus</em>. Only chloroform fraction showed medium inhibition activity against <em>Staphylococcus aureus</em>. The HPLC chemical profiles of the fractions were then correlated to their antibacterial activity profile by means of Orthogonal Projection to Latent Sructure (OPLS) analysis. Peak area with retention time of 0.96 - 1.12 min present in chloroform fraction at UV 250 nm was found to significantly correlate to the antibacterial activity (Y related coefficient value 0.73).


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