scholarly journals Rosella Flower Dyes as A Substitute for Fuchsin

2020 ◽  
Vol 3 (1) ◽  
pp. 25-28
Author(s):  
Sundari Sundari
Keyword(s):  
Staphylococcus Aureus ◽  
Natural Dyes ◽  
Synthetic Dyes ◽  
Hibiscus Sabdariffa ◽  
In Vitro Method ◽  
Flower Extract ◽  
Gram Staining ◽  
Degree Of Stability ◽  
Bacterial Preparations

Along with the needs of quality preparations, of course the ingredients needed must be good, except  the coloring preparations. Dyes that are widely used in practical activities are synthetic dyes.  However, natural dyes from plants are preferred due to the efficiency in their use while working with the same function. Natural dyes are safer to use even though the degree of stability to heat, light and acidity is uncertain. The purpose of this study was to determine if rosella flower extract (Hibiscus sabdariffa) could be used as a substitute for fuchsin in Gram staining. This research was conducted by the experiments with laboratory scale using in vitro method. The results of the study concluded that rosella flower extract could not be used as natural dye for bacterial staining particularly for Staphylococcus aureus and E.coli bacterial preparations.

Teratogenicity of Staphylococcus aureus L-forms using a mouse whole-embryo culture model

2013 ◽  
Vol 62 (5) ◽  
pp. 677-682
Author(s):  
Yong Liu ◽  
Xiang Zhu ◽  
Feng-ling Yu ◽  
Xiao-ming Kong ◽  
Na Lin ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Growth And Development ◽  
Mouse Embryos ◽  
Head Length ◽  
Control Group ◽  
Whole Embryo Culture ◽  
Crown Rump Length ◽  
Gram Staining ◽  
Pass Through

Our previous studies have suggested that Staphylococcus aureus L-forms are able to pass through the placental barrier of mice from the maternal side to the fetal body and affect fetal growth and development, but little is known about the direct influence of S. aureus L-forms on embryos during the critical period of organogenesis. Mouse embryos at gestational day 8.5 were cultured in vitro for 48 h with 0, 50, 100, 200 or 400 c.f.u. S. aureus L-forms ml−1. At the end of the culture period, the mouse embryos were assessed morphologically for viability, growth and development. Bacteriological and immunohistochemical staining were used to determine the existence of S. aureus L-forms in embryonic tissues. We found that both crown–rump length and head length of mouse embryos exposed to S. aureus L-forms at a concentration of 50 c.f.u. ml−1 were reduced. When the mouse embryos were exposed to 100, 200 or 400 c.f.u. S. aureus L-forms ml−1, the total morphological score, number of somites, dry embryo weight, yolk sac diameter, crown–rump length and head length were significantly lower than those of the control group. With the increased concentration of S. aureus L-forms in the culture medium, there were fewer normally developed embryos and more embryos with abnormalities or retardation in growth. S. aureus L-forms detected by Gram-staining and immunohistochemical detection of antigen were found in the tissues of embryos infected by S. aureus L-forms. These data suggest that S. aureus L-forms exert a direct teratogenic effect on cultured mouse embryos in vitro.

scholarly journals Protective Signatures of Roselle (Hibiscus sabdariffa L.) Calyx Fractions against Staphylococcus aureus in Drosophila Infection Model

2020 ◽  
Vol 27 (4) ◽  
pp. 306
Author(s):  
Firzan Nainu ◽  
M. Natsir Djide ◽  
Subehan Subehan ◽  
Sartini Sartini ◽  
Tri Puspita Roska ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Early Death ◽  
Luciferase Reporter ◽  
Infection Model ◽  
Hibiscus Sabdariffa ◽  
Immune Stimulation ◽  
Wild Type ◽  
Toll Pathway

The rise of antibiotic-resistant Staphylococcus aureus-related clinical cases is an alarming chronicle for global communities. This research was conducted to examine the antistaphylococcal effect of roselle (Hibiscus sabdariffa L.) calyx fractions in the Drosophila model. In the infection experiment, wild-type and immunodeficient Drosophila were pricked with S. aureus and subsequently subjected to fly survivorship and colony-forming assays, in the presence or absence of roselle calyx fractions. The Involvement of immune stimulation in the host antibacterial protection was assessed in vitro using cell-based luciferase reporter assay and in vivo using RT-qPCR analysis on adult flies. A declining rate of fly survivorship and augmentation of bacterial growth were observable in S. aureus-infected wild-type flies but subject to improvement in the presence of roselle calyx fractions. Cell-based analysis revealed the absence of host immune stimulation via Drosophila Toll pathway and roselle calyx fractions-treated immune-deficient flies lacking for components in the Toll pathway were protected from infection-induced early death phenotype and harbored reduced number of S. aureus colonies. Overall, our data confirmed the in vivo anti-staphylococcal activity of roselle calyx fractions in Drosophila infection model and such protective signature was devoid of host immune stimulation.

In vitro efficacy of Daptomycin against clinical isolates of Methicillin-resistant Staphylococcus aureus (MRSA)

2020 ◽  
pp. 1-9
Author(s):  
Asim Ali Shah ◽  
Shahid Ahmad Abbasi ◽  
Yasir Ali ◽  
Ayesha Maqbool
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sampling Technique ◽  
Staining Technique ◽  
Cross Sectional Study ◽  
Clinical Isolates ◽  
Cross Sectional ◽  
Methicillin Resistant ◽  
Gram Staining

Abstract This descriptive cross-sectional study was performed in the Department of Microbiology, Fauji Foundation Hospital, Rawalpindi, from March 2019 to September 2019 to determine the in vitro efficacy of Daptomycin against clinical isolates of Methicillin-Resistant Staphylococcus aureus (MRSA). Consecutive non-probability sampling technique was used and a total number of 270 patients’ Pan Cultures having MRSA growth on Cefoxatin Disc with size less than 22 mm zone size were included in the study. Cultures were inoculated on MacConkey, Chocolate and Blood agar and then incubated for 24 hours at 37 degree Celsius. After incubation, Coagulase test, Catalase test and Gram staining technique were used for further identification. Minimum Inhibitory Concentration (MIC) of the isolates for Daptomycin was obtained by using E strips (Oxoid UK) according to Clinical & Laboratory Standards Institute (CLSI) guidelines. Continuous...  

scholarly journals Antibacterial efficacy of Rosella (Hibiscus sabdariffa Linn) flower extract against Streptococcus sanguis

2015 ◽  
Vol 27 (1) ◽  
Author(s):  
Durotun Nafisa ◽  
Warta Dewi ◽  
Emma Rachmawati
Keyword(s):  
Ethanol Extract ◽  
Bacterial Activity ◽  
Diffusion Method ◽  
Hibiscus Sabdariffa ◽  
Streptococcus Sanguis ◽  
Flower Extract ◽  
Gram Staining ◽  
Inhibition Zones ◽  
Anaerobic Environment ◽  
Research Show

Introduction: Rosella is well known as health drink that contains anti bacterial compounds. The purpose of this research is to examine the anti bacterial potential of rosella calyx ethanol extract towards Streptococcus sanguis. Methods: Streptococcus sanguis was obtained from students’ saliva in the clinic of the Faculty of Dentistry, Universitas Padjadjaran. It was cultured in blood agar and incubated for 18 to 24 hours at 37°C in a facultative anaerobic environment. Streptococcus sanguis was isolated based on the characteristics of the colonies formed, Gram staining, inulin and rafinose fermentation tests. The anti bacterial test was performed using agar diffusion method (Kirby Bauer Method) by making agar holes in the agar and filling them with rosella extract with concentrations at 0.20 g/ml, 0.10 g/ml, 0.05 g/ml and 0.025 g/ml. Results: The results of this research show that there was anti bacterial activity from the ethanol extract of rosella calyx with the inhibition zones of 19.85 mm, 12.05 mm, 8.45 mm and 3.65 mm under rosellas’s extract’s concentration of at 0.20 g/ml, 0.10 g/ml, 0.05 g/ml and 0.025 g/ml respectively. Conclusion: The conclusion of this research is rosella calyx ethanol extract has the greatest anti bacterial activity at concentration 0.20 g/ml.

scholarly journals Antibacterial Activities of Pseudomonas orientalis APD 16 Isolate Sponge-Associated Aplysina sp. from Enggano Island Against Escherichia coli and Staphylococcus aureus

2020 ◽  
Vol 1 (2) ◽  
pp. 62-67
Author(s):  
Risky Hadi Wibowo
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Sea Water ◽  
Structural Complexity ◽  
Antibacterial Activities ◽  
Morphological Characters ◽  
Filter Feeder ◽  
Gram Staining ◽  
And Staphylococcus Aureus

Sponge is one of the invertebrates from the Porifera phylum. Sponge bodies have structural complexity with different cell layers. The sponge has many pores (ostium) on the surface of their body as a filter feeder. Sponge is recognized as organisms that have the potential because they can produce metabolites. Secondary metabolites produced by sponges are the result of the association of sponges with bacteria. The sponge used in this study is the Aplysina sp. sponge collected from Enggano island, Bengkulu Province. Aplysina sp. sponge is known to contain metabolites with antibacterial, antifungal and cytotoxic activity on cancer cells. This study aims to identify of potential isolate associated with Aplysina sp. sponge collected from Enggano island. Isolation of bacteria from Aplysina sp. sponge using Sea Water Complete (SWC) media. The isolate was screened by antagonistic test, morphological characters, Gram-staining, biochemical test and molecular identification. Based on the antagonistic test, APD 16 isolate could inhibit Escherichia coli and Staphylococcus aureus in Vitro. APD 16 isolate was identified molecularly using of 16S rRNAgenes analysis and it genetically close with Pseudomonas orientalis.

A in Vivo Assay for Standardizing Heparin Preparations

1979 ◽  
Vol 41 (03) ◽  
pp. 576-582
Author(s):  
A R Pomeroy
Keyword(s):  
In Vitro Assays ◽  
British Pharmacopoeia ◽  
In Vitro Method ◽  
Standard Preparation ◽  
Reliable Estimation ◽  
Assay Procedure ◽  
Accuracy And Precision ◽  
Heparin Preparation

SummaryThe limitations of currently used in vitro assays of heparin have demonstrated the need for an in vivo method suitable for routine use.The in vivo method which is described in this paper uses, for each heparin preparation, four groups of five mice which are injected intravenously with heparin according to a “2 and 2 dose assay” procedure. The method is relatively rapid, requiring 3 to 4 hours to test five heparin preparations against a standard preparation of heparin. Levels of accuracy and precision acceptable for the requirements of the British Pharmacopoeia are obtained by combining the results of 3 to 4 assays of a heparin preparation.The similarity of results obtained the in vivo method and the in vitro method of the British Pharmacopoeia for heparin preparations of lung and mucosal origin validates this in vivo method and, conversely, demonstrates that the in vitro method of the British Pharmacopoeia gives a reliable estimation of the in vivo activity of heparin.

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