scholarly journals Effectivity of Hand Sanitizer Cempaka Flower Extract (Michelia champaca L.) on the Growth of Staphylococcus aureus

2021 ◽  
Author(s):  
Sri Jayanthi ◽  
Nuraini ◽  
Setyoko
Keyword(s):  
Staphylococcus Aureus ◽  
Hand Sanitizer ◽  
Flower Extract ◽  
Michelia Champaca

scholarly journals A Potent Staphylococcus Aureus Growth Inhibitor Of A Dried Flower Extract Of Pinus Merkusii Jungh & De Vriese And Copper Nanoparticle

2018 ◽  
Vol 299 ◽  
pp. 012072 ◽  
Author(s):  
Masruri Masruri ◽  
Dinna Norani Pangestin ◽  
Siti Mariyah Ulfa ◽  
Slamet Riyanto ◽  
Arie Srihardyastutie ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibitor ◽  
Copper Nanoparticle ◽  
Flower Extract ◽  
Pinus Merkusii

scholarly journals ANTIBACTERIAL ACTIVITY AND THIN LAYER CHROMATOGRAPHY PROFILE Muntingia Calabura LEAF EXTRACT AS A MATERIAL CANDIDATE HAND SANITIZER

2018 ◽  
Vol 11 (1) ◽  
pp. 43
Author(s):  
Santy Pristianingrum ◽  
Baiq Lely Zainiati ◽  
Iwan Doddy Dharmawibawa
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Preventive Action ◽  
Hand Sanitizer ◽  
Layer Chromatography ◽  
Active Substances ◽  
Muntingia Calabura

Abstract : the utilization of antibacterial active substances from several plants is increasingly not only as the ingredients of medicine, but its utilization is also utilized for an antibacterial ingredient for preventive action, one of them is hand sanitizer material. This research focused to find the antibacterial active substances alternative from Muntingia calabura leaves extract. The data were analyzed descriptively including the inhibitory of ethanol absolute extract and ethanol 95% of M. Calabura against isolate clinical bacteria by Kirby Bauer method and the type of coumpund that contains in M.calabura leaf by thin layer chromatography utilizingeluen n-hexan- Methanol. Etanol absolute extract M.calabura leaf showing the average inhibition zone against Pseudomonas aeruginosa 15.67 mm, Staphylococcus aureus 19.33 mm and Escherichia coli 13 mm. While, The etanol extract 95% showing higher inhibition Pseudomonas aeruginosa 19.67 mm, Staphylococcus aureus 19.33 mm and Escherichia coli 16.67 mm. This inhibitory zone was slightly lower than chlorhexidine gluconate with an average of 20-24 mm against the three bacteria that utilized in the test but belongs to the strongly sensitive category for natural materials according to Mukherjee (1988). From thin layer chromatography profile with eluen n-hexan: methanol found three compounds that were in the range of Rf value 0.4; 0.5 and 0.7. The Conclusion for this study is the bioactive material from etanol 95% extract M.calabura leaf can be optimized to the hand sanitizer active compound candidate.

scholarly journals Hand Sanitizer Ekstrak Daun Pucuk Idat (Cratoxylum glaucum) sebagai Antibakteri Staphylococcus aureus dan Escherichia coli

2020 ◽  
Vol 16 (2) ◽  
pp. 227
Author(s):  
Megawati Ayu Putri ◽  
Marhan Ebit Saputra ◽  
Ike Nur Amanah ◽  
Siska Musiam ◽  
Verry Andre Fabiani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Skin Irritation ◽  
Test Results ◽  
Antibacterial Test ◽  
E Coli ◽  
Hand Sanitizer ◽  
Carboxymethyl Cellulose Sodium

<p><span id="docs-internal-guid-2cebd816-7fff-d997-4dfd-796d046d0d3b"><span>Daun pucuk idat merupakan tanaman lokal Bangka Belitung yang memiliki aktivitas antibakteri terhadap </span><span>Staphylococcus aureus </span><span>dan</span><span> Escherichia coli.</span><span>Sebagai upaya pemanfaatan potensi lokal maka dibuat suatu produk sediaan gel </span><span>hand sanitizer</span><span> yang praktis dan ekonomis. </span><span>Ekstrak daun pucuk idat diperoleh dengan metode maserasi menggunakan pelarut aseton. Ekstrak yang dihasilkan diformulasikan dalam berbagai konsentrasi ekstrak daun pucuk idat (40%, 60%, 80%, dan 100%) dengan penambahan </span><span>carboxymethyl cellulose sodium</span><span> (</span><span>CMC-Na) 0,25 g, gliserin 1 mL, propilenglikol 0,5 mL serta akuades        10 mL. Setiap formulasi gel </span><span>handsanitizer</span><span> dilakukan pengujian stabilitas fisik meliputi uji organoleptis, uji homogenitas, uji daya sebar, uji pH, uji iritasi kulit serta uji aktivitas antibakteri.</span><span> </span><span> Hasil uji organoleptik menunjukkan bahwa semua konsentrasi </span><span>hand sanitizer </span><span>berbentuk gel, berwarna kuning muda, berbau dan homogen. Uji pH dan daya sebar gel</span><span> hand sanitizer</span><span> telah memenuhi ketentuan SNI No. 06-2588. Pengujian iritasi kulit tidak menimbulkan kemerahan, gatal-gatal dan tidak mengakibatkan kulit menjadi kasar pada punggung tangan sukarelawan. Berdasarkan uji sifat fisik gel</span><span> hand sanitizer </span><span>yang dihasilkan memiliki sifat fisik yang serupa dengan gel </span><span>hand sanitizer </span><span>komersial. Hasil uji antibakteri hand sanitizer ekstrak daun pucuk idat terhadap </span><span>E. coli</span><span> dengan konsentrasi ekstrak daun pucuk idat 40%, 60%, 80%, dan 100% diperoleh diameter zona hambat berturut-turut sebesar 11,5 mm, 17,4 mm, 19,3 mm dan 19,3 mm, sedangkan pada bakteri </span><span>S. aureus</span><span> diperoleh diameter zona hambat berturut-turut sebesar 17,1 mm, 24,2 mm, 27,4 mm, dan  18 mm. Hasil uji antibakteri menunjukkan bahwa sediaan gel </span><span>hand sanitizer </span><span>ekstrak aseton daun pucuk idat memiliki aktivitas antibakteri terhadap bakteri </span><span>E. coli </span><span>dan </span><span>S. aureus</span><span>.</span></span></p><p><span><span><span id="docs-internal-guid-50d6ff2c-7fff-2e66-45f1-73cf49e6527a"><strong>Hand Sanitizer of Leaves Extract of Cratoxylum glaucum as Antibacterial of Staphylococcus aureus and Escherichia coli. </strong><span>Cratoxylum glaucum</span><span> leaves is local plants of Bangka Belitung which have antibacterial activity against </span><span>S. aureus</span><span> and </span><span>E. coli</span><span>. As an effort to explore local potential, a practical and economical gel hand sanitizer product was prepared. </span><span>Cratoxylum glaucum</span><span> leaves extract was obtained by maceration method using acetone. The extract was formulated in various concentrations of </span><span>Cratoxylum glaucum</span><span> leaf extract (40%, 60%, 80%, and 100%) with the addition of 0.25 g carboxymethyl cellulose sodium (CMC-Na), 1 mL glycerin, 0.5 mL propylene glycol and 10 mL distilled water. Each concentration of the hand sanitizer gel was tested for physical stability including organoleptic, homogenity, spreadability, pH, skin irritation and antibacterial activity test. The organoleptic test results showed that all concentrations of hand sanitizers formed gel with light yellow color, smelly odor and homogeneous form. The analysis of pH and spreadability of hand sanitizer gel has fulfilled SNI No. 06-2588. The skin irritation testing does not cause redness, itching, and rough skin on the back of volunteer hands. Based on the physical properties test, the resulting hand sanitizer gel has physical properties similar to commercial hand sanitizer gel. The results of the antibacterial test of hand sanitizer against </span><span>E. coli</span><span> with </span><span>Cratoxylum glaucum</span><span> leaf extract concentrations of 40%, 60%, 80%, and 100% obtained the diameter inhibitory zones of 11.5 mm, 17.4 mm, 19.3 mm, and 19.3 mm, respectively. Meanwhile, the results of the antibacterial test of hand sanitizer against </span><span>S. aureus</span><span> bacteria with concentration of 40%, 60%, 80%, and 100% shows that the inhibition zone diameters were 17.1 mm, 24.2 mm, 27.4 mm, and 18 mm, respectively. The antibacterial test results shows that the gel hand sanitizer prepared has antibacterial activity against </span><span>E. coli</span><span> and </span><span>S. aureus</span><span> bacteria.</span></span></span></span></p>

scholarly journals Characterization of Antimicrobial, Antioxidant, Anticancer Property and Chemical Composition of Michelia champaca Seed and Flower Extracts

1970 ◽  
Vol 4 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Lee Seong Wei ◽  
Wendy Wee ◽  
Julius Young Fu Siong ◽  
Desy Fitrya Syamsumir
Keyword(s):  
Antimicrobial Property ◽  
Radical Scavenging ◽  
Chemical Compounds ◽  
Anticancer Activities ◽  
E Coli ◽  
Flower Extract ◽  
Microdilution Method ◽  
Ic50 Value ◽  
Michelia Champaca

This study was carried out to characterize antimicrobial, antioxidant and anticancer activities of Michelia champaca seed and flower extracts. The main objective of the present study was to reveal the medicinal values of M. champaca seed and flower for human uses. Antimicrobial property of M. champaca seed and flower extracts were revealed by using two fold microdilution method whereas antioxidant activity of the extract was determined with DPPH radical scavenging method. The anticancer property of the plant extract was revealed through Colorimetric MTT (tetrazolium) assay. The minimum inhibitory concentration values of M. champaca seed and flower extracts ranged from 15.6 to 125mg/l and 7.8 to 62.5mg/l, respectively in which both of the plant extracts were found can inhibit the growth of all the tested bacterial isolates namely A. hydrophila, E. tarda, E. coli, Flavobacterium sp., Klebsiella sp., P. aeruginosa, Salmonella sp., V. alginolyticus, V. cholerae and V. parahaemolyticus. M. champaca flower extract was able to control the growth of E. tarda, E. coli, Flavobacterium sp., P. aeruginosa and V. cholerae at the concentration of 7.8mg/l whereas A. hydrophila, Klebsiella sp. and V. alginolyticus were failed to grow at the concentration 15.6mg/l. The M. champaca flower extract was also able to control the growth of Salmonella sp. and V. parahaemolyticus at the concentration of 62.5mg/l. At the maximum concentration of M. champaca seed and flower extracts were found can inhibit only 40% of DPPH whereas the IC50 value of M. champaca seed and flower extract against MCF-7 cells was 1.98 ±0.31μg/ml and 1.86 ± 0.21μg/ml, respectively. A total of 9 chemical compounds were successfully identified in M. champaca’s flower extract whereas 37 chemical compounds were found in the leaf extract. The findings of the present study indicated that medicinal values of M. champaca seed & flower extracts in terms of antimicrobial and anticancer are promising.Key words: Antioxidant; Anticancer; Antimicrobial; Chemical compound; Michelia champaca DOI: http://dx.doi.org/10.3329/sjps.v4i1.8862 SJPS 2011; 4(1): 19-24

scholarly journals PEMANFATAN EKSTRAK BIJI TERATAI SEBAGAI BAHAN AKTIF ANTIBAKTERI UNTUK PEMBUATAN HAND SANITIZER

2019 ◽  
Vol 3 (1) ◽  
pp. 7
Author(s):  
Farindya Dwi Cahyaningtyas ◽  
Zhilla Afifatul Ukrima ◽  
Nora Nora ◽  
Amaria Amaria
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Hand Sanitizer

Biji teratai memiliki kandungan komponen  fitokimia seperti alkaloid, flavonoid, steroid, glikosida, saponin, tannin, dan triterpenoid. alkaloid, flavonoid, steroid, glikosida, saponin, tannin, dan triterpenoid yang terdapat pada biji teratai seperti flavonoid, alkaloid, tannin,  dapat digunakan sebagai senyawa antibakteri. Penelitian ini bertujuan mengetahui aktivitas antibakteri  gel hand sanitizer ekstrak etil asetat biji teratai. Penelitian  diawali dengan ekstraksi biji teratai kering dengan cara maserasi selama 3 hari  menggunakan etil asetat.  Maserat yang dihasilkan selanjutnya dievaporasi menggunakan rotatory evaporator dan kandungan pelarut etil asetat dihilangkan dengan menggunakan waterbath dengan menjaga suhunya <60oC. Selanjutnya dilakukan proses pembuatan sediaan gel. Sediaan gel yang dihasilkan kemudian dilakukan pengujian aktivitas antibakteri sediaan pada Escherichia coli dan Staphylococcus aureus uji stabilitas sediaan gel hand sanitizer yang terdiri dari uji pH, uji daya sebar,  uji sineresis, serta uji organoleptis meliputi warna, bau, dan bentuk. Hasil penelitian menunjukkan bahwa semakin banyak ekstrak biji teratai yang ditambahkan, warna yang dihasilkan semakin gelap, aroma yang semakin kuat,  bentuk yang tidak kental, serta daya sebar yang semakin lebar. pH formula dari F1, F2,  F3 berturut-turut yaitu 6,4 ; 5,9 ; 5,1. Sediaan gel hand sanitizer tidak sineresis. Aktivitas antibakteri hand sanitizer ekstrak biji teratai yang dapat menghambat atau mematikan bakteri dengan sangat baik pada konsentrasi 1,5 % yang menghasilkan zona hambat sebesar 12 mm pada bakteri  Staphylococcus aureus dan 10 mm pada bakteri  Escherichia coli.

scholarly journals Rosella Flower Dyes as A Substitute for Fuchsin

2020 ◽  
Vol 3 (1) ◽  
pp. 25-28
Author(s):  
Sundari Sundari
Keyword(s):  
Staphylococcus Aureus ◽  
Natural Dyes ◽  
Synthetic Dyes ◽  
Hibiscus Sabdariffa ◽  
In Vitro Method ◽  
Flower Extract ◽  
Gram Staining ◽  
Degree Of Stability ◽  
Bacterial Preparations

Along with the needs of quality preparations, of course the ingredients needed must be good, except  the coloring preparations. Dyes that are widely used in practical activities are synthetic dyes.  However, natural dyes from plants are preferred due to the efficiency in their use while working with the same function. Natural dyes are safer to use even though the degree of stability to heat, light and acidity is uncertain. The purpose of this study was to determine if rosella flower extract (Hibiscus sabdariffa) could be used as a substitute for fuchsin in Gram staining. This research was conducted by the experiments with laboratory scale using in vitro method. The results of the study concluded that rosella flower extract could not be used as natural dye for bacterial staining particularly for Staphylococcus aureus and E.coli bacterial preparations.

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