Incidence of Enteropathogenic Escherichia coli among Infants at Eleyele Comprehensive Health Centre, Ile-Ife, Osun State

2019 ◽  
Vol 152 (Supplement_1) ◽  
pp. S130-S131
Author(s):  
Kayode Faseyi ◽  
Jaiyeola Onifade
Keyword(s):  
Escherichia Coli ◽  
Teaching Hospital ◽  
Health Centre ◽  
Age Groups ◽  
Developed Countries ◽  
University Teaching ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
Total Percentage ◽  
Comprehensive Health

Abstract Objectives This work was carried out to find the incidence of enteropathogenic Escherichia coli in children below 5 years of age who complained of having diarrhea at Eleyele Comprehensive Health Centre, Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife. The work was also done to determine the incidence in different age groups of both sexes. Methods All specimens were cultured as routinely done at the bacteriology laboratory in Obafemi Awolowo University Teaching Hospital for stool samples. Colonies of lactose fermenters were processed after overnight incubation at 37°C. Nonlactose fermenter colonies were discarded. Out of 200 colonies isolates on MacConkey agar, there were 150 lactose fermenters and these were all processed. The other 70, which were Gram-positive cocci, were discarded. These were subjected to biochemical examination such as treating them with sugars, for the production of acid and gas, indole production, motility with sugars, citrate utilization, Voges-Proskauer, and methyl red tests. Results Out of 150 strains examined, only 80 strains obeyed IMVic ++ -- reactions. These were serotyped to know the enteropathogenic strains. From the result obtained, 23 strains reacted positively with polyvalent antisera (polyvalent 2, 3, 4 antisera), of which 13 strains gave a positive reaction with corresponding monovalent antisera. There were five strains from polyvalent 2 antisera, five strains from polyvalent 3 antisera, and three strains from polyvalent 4 antisera. In close study of this work, total percentage of females with diarrhea was 44.5% while total percentage of males with diarrhea was 55.5%. Out of these 80, E coli was isolated, and from these, 13 enteropathogenic E strains were isolated (16.25%). Patient aged below 2 years gave nine strains (69.2%), aged 2 to 3 years gave two strains (15.4%), aged 3 to 4 years gave one strain (7.7%), and aged 4 to 5 years gave one strain (7.7%). Also, close examination of result revealed that male children had the greater percentage of enteropathogenic infection, with 8 strains out of the 13 strains serotyped (61.5%) and female children with 5 strains (38.5%). Conclusion E coli remains an important cause of infant diarrhea. Although epidemics of infant diarrhea caused by enteropathogenic E coli have almost disappeared from the developed countries, it is still very common in developing countries. The relative importance of enteropathogenic E coli as a cause of infant diarrhea needs to be reassessed and new diagnostic techniques will also help to simplify this task.

Incidence Of Enteropathogenic Escherichia Coli Among Infants At Eleyele Comprehensive Health Centre, Ile-Ife

2020 ◽  
Vol 154 (Supplement_1) ◽  
pp. S134-S134
Author(s):  
K T Faseyi
Keyword(s):  
Escherichia Coli ◽  
Teaching Hospital ◽  
Health Centre ◽  
Developed Countries ◽  
Diagnostic Techniques ◽  
University Teaching ◽  
Enteropathogenic Escherichia Coli ◽  
Total Percentage ◽  
Comprehensive Health ◽  
The Developed Countries

Abstract Introduction/Objective This work was carried out to find the incidence of enteropathogenic Escherichia Coli in children below five years of age who complained of having diarrhea at Eleyele Comprehensive Health Centre, Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife. Methods All specimen were cultured as routinely done in Bacteriology Laboratory in Obafemi Awolowo University Teaching Hospital for stool samples. Colonies of lactose fermenters were processed after overnight incubation at 37°c. Non lactose fermenter colonies were discarded. Out of 200 colonies isolates on MacConkey Agar, there were 150 lactose fermenters and these were all processed. Biochemical examination on sugars, for the production of acid and gas, indole production, motility, citrate utilization, voges-prokauer and Methyl red tests. Results Out of 150 strains, only 80 strains obeyed IMVic ++ -- reactions. These were serotyped to know the enteropathogenic strains. From the result obtained, 23 strains reacted positively with polyvalent antisera (Polyvalent 2, 3, 4 antisera) out of which 13 strains gave a positive reaction with corresponding monovalent antisera. 5 strains from polyvalent 2 antisera, 5 strains from polyvalent 3 antisera, and 3 strains from polyvalent 4 antisera. In close study of this work, total percentage of female with Diarrhea cases were 44.5% total percentage of male with diarrhea cases were 55.5%. Out of these 80, Escherichia coli were isolated, from these 13 enteropathogenic Escherichia coli strains were isolated (16.25%). Patient with age group below 2 years gave 9 strains 69.2%, 2-3 years gave 2 strains (15.4%), 3-4 years gave 1 strain (7.7%) and 4-5 years gave 1 strain (7.7%). Also close examination of result revealed that male children had the greater percentage of Enteropathogenic infection with 8 strains out of the 13 strains serotyped (61.5%) and female children with 5 strains (38.5%). Conclusion Escherichia coli remain an important cause of infant diarrhea. Although epidemics of infant diarrhea caused by enteropathogenic Escherichia coli have almost disappeared from the developed countries, it is still very common in developing countries. The relative importance of enteropathogenic Escherichia coli as a cause of infant diarrhea need to be reassessed and new diagnostic techniques will also help to simplify this task.

scholarly journals Molecular Characterization and Antimicrobial Resistance of Enteropathogenic Escherichia coli in Children from Ahvaz, Iran

2020 ◽  
Vol 13 (7) ◽  
Author(s):  
Mehrandokht Sirous ◽  
Mohammad Hashemzadeh ◽  
Maryam Keshtvarz ◽  
Mansour Amin ◽  
Nasim Shams ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Developed Countries ◽  
Therapeutic Strategies ◽  
Beta Lactamase ◽  
Enteropathogenic Escherichia Coli ◽  
Emerging Pathogen ◽  
E Coli ◽  
Atypical Strains ◽  
Developing And Developed Countries

Background: Enteropathogenic Escherichia coli (EPEC) is one of the most important pathogens among young children worldwide. Both eae and bfp genes have been used to identify EPEC strains and categorize them into typical and atypical strains. They may be an emerging pathogen in both developing and developed countries. Objectives: This study was primarily conducted to assess the epidemiology, drug resistance, and β-lactamase distribution of EPEC, as well as the detection of efa1/lifA in atypical strains. Methods: A total of 251 E. coli strains isolated from children with diarrhea were evaluated for their EPEC pathotype by PCR for the presence of eae, stx1, stx2, and bfp genes. Serogrouping with polyvalent antisera was performed to confirm EPEC strains. Atypical EPEC-containing samples were evaluated for the efa1/lifA gene. EPEC isolates were assessed to recognize the antibiotic resistance and screened to detect extended-spectrum β-lactamases (ESBLs). Results: Enteropathogenic E. coli strains were detected in 17 (6.78%) of E. coli isolates by PCR. The prevalence of typical and atypical strains was determined at 35.3% and 64.7%. All strains were completely susceptible to colistin, imipenem, and meropenem. The prevalence of blaCTX-M and blaTEM genes was calculated at 70.58% and 58.82%, respectively. Conclusions: Enteropathogenic E. coli isolates are completely sensitive to carbapenems, and precise therapeutic strategies are required to prevent the spread of these beta-lactamase genes among diarrheagenic E. coli.

scholarly journals Escherichia coli Antimicrobial Susceptibility Reduction amongst HIV-Infected Individuals at the University Teaching Hospital, Lusaka, Zambia

2020 ◽  
Vol 17 (10) ◽  
pp. 3355
Author(s):  
Freeman Chabala ◽  
Mutinta Madubasi ◽  
Mable Mwale Mutengo ◽  
Njeleka Banda ◽  
Kaunda Yamba ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Teaching Hospital ◽  
Antimicrobial Susceptibility ◽  
University Teaching ◽  
Health Concern ◽  
Hiv Positive ◽  
E Coli ◽  
Stool Samples ◽  
The University ◽  
Hiv Negative

Increased antimicrobial resistance among Human Immunodeficiency Virus (HIV)-infected individuals to commonly used antibiotics in the treatment of gastroenteritis is a public health concern, especially in resource-limited settings. We set out to compare the antimicrobial susceptibility pattern of Escherichia coli (E. coli) isolates from HIV-infected and HIV-uninfected individuals at a tertiary hospital in Lusaka, Zambia. An analytical cross-sectional study was conducted at the University Teaching Hospital from May 2019 to August 2019. Stool samples were screened, and 79 HIV-infected individuals matched by age and sex with 84 HIV-uninfected individuals that presented with E. coli associated gastroenteritis were studied. Demographics were collected from the Laboratory Information System (LIS) and stool samples were collected in a sterile leak-proof container. Samples were cultured and only those where E. coli was isolated were included in the study and tested for antimicrobial susceptibility by the Kirby–Bauer disk diffusion technique. HIV-positive individuals were 3 times (adjusted odds ratio (AOR) = 3.17; 95% CI (1.51, 6.66); p < 0.001) more likely to be resistant to quinolones compared with their HIV-negative counterparts. Similarly, HIV-positive individuals were almost 4 times (AOR = 3.97, 95% CI (1.37, 11.46); p = 0.011) more likely to have multidrug-resistant E. coli compared with those who were HIV-negative. HIV infection was associated with reduced E. coli susceptibility to commonly used antibiotics, and most cases showed resistance.

Molecular characterization of ESBL- producing uropathogenic Escherichia coli recovered from urine samples of patients attending a University Teaching hospital in Nigeria

2021 ◽  
Author(s):  
Abimbola Olumide Adekanmbi ◽  
Sandra Usidamen ◽  
Anthony Abiodun Onilude
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Teaching Hospital ◽  
Culture Collection ◽  
Urine Samples ◽  
University Teaching ◽  
Diffusion Method ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Esbl Producers

AbstractInfection of the urinary tract ranks as one of the most common infections affecting people worldwide and its treatment is made complicated by the rising incidence of antibiotic resistance. This study aimed to detect extended spectrum beta-lactamase (ESBL) genes and antibiotic resistance profile of uropathogenic Escherichia coli (E. coli) recovered from patients attending a University Teaching hospital in Nigeria. Uropathogenic E. coli isolates were obtained from the culture collection of Department of Microbiology and Parasitology of the University Teaching hospital for a period of four months (October 2019–January, 2020). Antibiotic susceptibility testing was done using the disc diffusion method while phenotypic ESBL production was detected using double disc synergy test (DDST). Detection of β-lactamase genes was done using Real-Time PCR. Forty-nine E. coli isolates were recovered from 120 urine samples, with 24 (49%) being ESBL positive. The resistance to antibiotics in the ESBL producers was: ciprofloxacin (100%), cefotaxime (100%), cefpodoxime (100%), tetracycline (95.7%), ceftazidime (56.7%), amoxicillin-clavulanate (50%), gentamicin (33.3%), and imipenem (0%). All the ESBL producers carried blaTEM, blaCTX-M-1 and blaCTX-M-9, 75% (18/24) carried blaSHV, while blaCTX-M-2, blaCTX-M-8 and blaCTX-M-25 groups were detected in 20.8% (5/24) of the isolates. There was co-occurrence of CTX-M, SHV and TEM β-lactamases in 79.2% (19/24) isolates, while five isolates (20.8%) co-harbored blaCTX-M and blaTEM. This study showed a high level of multidrug resistance and ESBL gene carriage in uropathogenic E. coli obtained in this study, suggesting a likely review of therapeutic options in the treatment of UTI to clamp down on the rising cases of antibiotic resistance.

Spheroplasts of enteropathogenic Escherichia coli. I. Biological characteristics

1968 ◽  
Vol 14 (6) ◽  
pp. 675-678 ◽  
Author(s):  
B. Diena ◽  
R. Wallace ◽  
L. Greenberg
Keyword(s):  
Escherichia Coli ◽  
Tissue Culture ◽  
Biological Characteristics ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
O Antigen ◽  
Pathogenic Serotypes

The properties of glycine-induced spheroplasts of six pathogenic serotypes of E. coli were investigated. Fimbriae and flagella appeared to be only partially synthesized as was the somatic O antigen. Cytopathogenicity of these spheroplasts for tissue culture was reduced and the infection of the monolayers was retarded as compared with the normal bacillary forms. Sensitivity to phage was almost completely lost, suggesting that glycine had either interfered with the synthesis of phage receptors or had altered the mucopeptide layerwhich is the substrate for phage enzymes. Alternatively, the phage may become a prophage inside the spheroplast with the loss of virulence.

Comparison of colony and stool blots for detection of enteropathogens by DNA probes

1991 ◽  
Vol 37 (5) ◽  
pp. 407-410
Author(s):  
Mônica A. M. Vieira ◽  
Beatriz E. C. Guth ◽  
Tânia A. T. Gomes
Keyword(s):  
Escherichia Coli ◽  
Key Words ◽  
Sensitivity And Specificity ◽  
Dna Probes ◽  
Type I ◽  
Enteropathogenic Escherichia Coli ◽  
Key Words Dna ◽  
E Coli ◽  
Heat Stable

DNA probes that identify genes coding for heat-labile type I (LT-I) and heat-stable type 1 (ST-I) enterotoxins, enteropathogenic Escherichia coli adherence factor (EAF), and Shigella-like, invasiveness (INV) are used to evaluate the sensitivity and specificity of stool blots in comparison with the sensitivity and specificity of colony blots in detecting enteropathoghens. The sensitivities of the probes in stool blots are 91.7% for the LT-I probe, 76.9% for the ST-I probes, 78.9% for the EAF probe, and 45.5% for the INV probe. The specificity of all probes is higher than 95%. In general, the stool blot method identifies as many if not more LT-I-, ST-I-, and EAF-producing E. coli infections than the colony blots. Key words: DNA probes, stool blots, enteropathogens, diagnosis.

scholarly journals Novel Hybrid of Typical Enteropathogenic Escherichia coli and Shiga-Toxin-Producing E. coli (tEPEC/STEC) Emerging From Pet Birds

2018 ◽  
Vol 9 ◽  
Author(s):  
Rosely Martins Gioia-Di Chiacchio ◽  
Marcos Paulo Vieira Cunha ◽  
Lilian Rose Marques de Sá ◽  
Yamê Minieiro Davies ◽  
Camila Bueno Pacheco Pereira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
Pet Birds

Diarrhea Associated With Adherent Enteropathogenic Escherichia coli in an Infant and Toddler Center, Seattle, Washington

PEDIATRICS ◽  
1986 ◽  
Vol 77 (3) ◽  
pp. 296-300
Author(s):  
Leonard J. Paulozzi ◽  
Kathleen E. Johnson ◽  
Lawrence M. Kamahele ◽  
Carla R. Clausen ◽  
Lee W. Riley ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Day Care ◽  
Care Center ◽  
The United States ◽  
Enteropathogenic Escherichia Coli ◽  
Day Care Center ◽  
E Coli ◽  
Published Report ◽  
The Common ◽  
Stool Cultures

During November 1983, the Seattle-King County Department of Public Health investigated an outbreak of diarrhea associated with enteropathogenic Escherichia coli, serogroup 0111:K58, in an infant and toddler day-care center. Of the 25 children in the center, ranging in age from 4 to 30 months (median age 11 months), diarrhea occurred in 14 characterized by watery, greenish stools. The median duration of diarrhea was 12 days. Two of the ill children were hospitalized because of severe dehydration. Stool cultures from the children diagnosed initially did not yield the common bacterial pathogens, parasites, or rotavirus. Stool cultures from 11 of 14 ill children and two of 11 well children (P &lt;.005), however, yielded an E coli serogroup, 0111: K58, which was not invasive or toxigenic by standard tests. The source of the organism was not identified. Although this organism has been recognized as a cause of diarrhea in newborn nurseries, this is the first published report of a documented outbreak of enteropathogenic E coli-induced diarrhea in a day-care center in the United States.

Methodology for Enteropathogenic Escherichia coli

1975 ◽  
Vol 58 (2) ◽  
pp. 283-292
Author(s):  
Ira J Mehlman ◽  
Nicholas T Simon ◽  
Arvey C Sanders ◽  
Morris Fishbein ◽  
Joseph C Olson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Elevated Temperature ◽  
Growth Temperature ◽  
Maximal Growth ◽  
Enteropathogenic Escherichia Coli ◽  
Pathogenic Potential ◽  
Gram Negative ◽  
Tentative Identification ◽  
E Coli ◽  
Capsular Antigens

Abstract Pathogenic biotypes of Escherichia coli grow poorly at temperatures greatly different from that of the host. Percentages quantitatively recovered at 42.0, 44.0, 44.5, and 45.5°C in lauryl tryptose broth were 100, 76, 76, and 42, respectively. Corresponding values for 175 strains of varied origin were 98, 89, 82, and 65%. Maximal growth temperature is dependent upon medium. Lauryl tryptose and elevated coliform broths were equivalent in the recovery of small inocula (100 cells/ml) at 41.5–44.5°. Mac-Conkey, enteric enrichment, and Gram-negative broths were inhibitory at corresponding values. Growth at elevated temperature in nutrient broth is enhanced by carbohydrate. Standard lactose enrichment media fail to recover slow lactose fermenters. An acidified glutamic acid medium was unsuitable for recovery of E. coli. The data suggest modification of standard temperatures for the recovery of pathogenic biotypes. Previously recommended analytical methods have been simplified and supplemented. The enhancement of motility in indole-nitrite broth at 35°C is recommended. A 4-tube semiquantitative test is offered for tentative identification of somatic and capsular antigens. Inclusion of Alkalescens-Dispar strains is warranted by their pathogenic behavior. Examination in Shigella and Alkalescens-Dispar sera is required to cover the dysentery-like biotypes. Pathogenic potential cannot be inferred from serotype.

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