A Pilot Study in Humans to Investigate the Relationship Between Circulating L-citrulline and Arginine After Watermelon Intake (rind, Flesh and Seeds) on Endothelial Function (P06-121-19)
Abstract Objectives Endothelial dysfunction is one of the early predictors of cardiovascular disease and proper functioning of the endothelium is dependent upon nitric oxide (NO) bioavailability. Watermelon is a rich source of bioactive components such as L-citrulline and arginine which can increase NO bioavailability directly and indirectly. The objective of this pilot study was to assess the effects of watermelon intake on endothelial function using Flow Mediated Dilation (FMD) and to assess the relationship of FMD overtime with circulating watermelon metabolites, such as L-citrulline and arginine in humans. Methods Middle-age overweight/obese adults (n = 6; age 32.4± 8.0 y and BMI 29.0 ± 1.7 kg/m2) participated in a 4-arm, randomized, cross-over energy-controlled clinical trial. Subjects consumed one of four 100 kcal salads containing watermelon flesh (WF) or rind (WR) or seed (WS) or no watermelon components (Control) on four separate occasions. Blood samples were collected at baseline (t = 0 h) and then 0.5 h, 1 h, 2 h, 3 h, 4 h, 5 h, 6 h, 7 h, 8 h, 24 h post meal. The FMD was assessed at baseline 0 h, 1 h, 3 h, 5 h and 7 h. L-Citrulline and arginine were extracted from plasma at each time point and quantified using an ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometer. Results Maximum concentration (Cmax) and peak time (Tmax) for L-citrulline after WF intake was 153.1 ± 38.8 µmol/L with Tmax of 1 h while after WR intake Cmax was 153.9 ± 38.5 µmol/L with tmax of 0.5 h. Cmax of L-citrulline was 76.1 ± 20.9 µmol/L at time 1 h after WS consumption. Arginine peaked at 1 h after WF, WR, WS intake with Cmax of 242.5 ± 35.8 µmol/L, 249.9 ± 38.7 µmol/L, and 249.5 ± 43.3 µmol/L respectively. An increase in %FMD was observed at 1 h after WF and WR compared to control, which coincided with the Cmax and Tmax of arginine and citrulline. However, the data was statistically not significant. Conclusions The data from this pilot study is the first to show a possible association between %FMD increase and circulating L-citrulline and arginine concentrations providing support for future research on watermelon with a larger sample size in order to determine the potential clinical impact of watermelon intake on improvement of endothelial function. Funding Sources National Watermelon Promotion Board.