Enzymatic Kinetic Rate and End-Point Analyses of Substrate, by Use of a GeMSAEC Fast Analyzer

1972 ◽  
Vol 18 (8) ◽  
pp. 829-840 ◽  
Author(s):  
T O Tiffany ◽  
J M Jansen ◽  
C A Burtis ◽  
J B Overton ◽  
C D Scott
Keyword(s):  
Clinical Chemistry ◽  
Fixed Time ◽  
Small Computer ◽  
Time Analysis ◽  
Kinetic Rate ◽  
End Point ◽  
Unique Method ◽  
On Line ◽  
The Cost ◽  
Multiple Samples

Abstract Enzymatic substrate analysis is an attractive means of analysis in clinical chemistry because of its sensitivity and specificity. The GeMSAEC Fast Analyzer, in conjunction with a small computer, provides a means of performing routine enzymatic substrate analysis and offers the following advantages: (a) selectivity of approaches to enzymatic analysis, i.e., end-point or kinetic; (b) essentially parallel analyses of multiple samples, yielding a unique method for performing kinetic fixed-time analysis; (c) on-line data reduction, resulting in rapid calculation and output of results and the minimization of data handling errors; and (d) a small reagent volume per test (400 µl), which reduces the cost of analysis. The analysis of substrate with enzymatic end-point and kinetic procedures is examined by use of a computer-interfaced Fast Analyzer. Computer programs were written to facilitate this study. Glucose (hexokinase/GPD), urea (urease/GMD), and uric acid (uricase) have been used as examples in evaluating both end-point and kinetic analyses. The advantages and limitations of each type of analysis are presented, with the emphasis being placed on enzymatic substrate analysis and means by which the computer-interfaced Fast Analyzer can facilitate both end-point and kinetic analyses.

scholarly journals Optimization on reagent-loading manner for modular clinical chemistry analyzer series: simulations and verifications

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Mingyang Wang ◽  
Liang Ming
Keyword(s):  
Quality Control ◽  
Clinical Chemistry ◽  
Turnaround Time ◽  
Laboratory System ◽  
Related Factors ◽  
Loading Mode ◽  
Mode Pattern ◽  
On Line ◽  
And Performance ◽  
The Cost

AbstractObjectivesThe pre- and post-analytical processes have been discussed both in total laboratory system (TLA) and modular automation (MA). The analytical process, especially reagent-related factors influences on the integrated clinical chemistry analyzer, demonstrates a significant effect on clinical chemistry analyzer. Modular analyzer reagent-loading mode influences two mainly factors, testing turnaround time (tTAT) and the cost. Furthermore, how to definite the different reagent loading manners and verify the best reagent loading manner is big challenge.MethodsWe focus on tTAT, and study how the reagent-related factors effect TAT by simulations and verifications. Parameters were simulated by cobas 8000 workflow simulator for reagent-loading manner with at least three positions (Pattern 1), the module-parallel reagent-loading manner (Pattern 2) and the single-position loading mode (Pattern 3).ResultstTAT, reagent on-line time, quality control (QC) cost and performance verification times all declined by 43%. Tuesday effect solved the repetitive problem for verification. Pattern 2 shows optimal performance in Tuesday effect-based verification.ConclusionsThe optimization of reagent-loading manner saved much workforce, and reduced the QC cost.

Image registration with a computer driven S.T.E.M.

1978 ◽  
Vol 36 (1) ◽  
pp. 98-99
Author(s):  
A.M.H. Schepman ◽  
J.A.P. van der Voort ◽  
J.E. Mellema
Keyword(s):  
Spot Size ◽  
Scanning Transmission Electron Microscope ◽  
Small Computer ◽  
Structural Studies ◽  
Area Of Interest ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Specimen Area ◽  
On Line ◽  
Minimum Distances

A Scanning Transmission Electron Microscope (STEM) was coupled to a small computer. The system (see Fig. 1) has been built using a Philips EM400, equipped with a scanning attachment and a DEC PDP11/34 computer with 34K memory. The gun (Fig. 2) consists of a continuously renewed tip of radius 0.2 to 0.4 μm of a tungsten wire heated just below its melting point by a focussed laser beam (1). On-line operation procedures were developped aiming at the reduction of the amount of radiation of the specimen area of interest, while selecting the various imaging parameters and upon registration of the information content. Whereas the theoretical limiting spot size is 0.75 nm (2), routine resolution checks showed minimum distances in the order 1.2 to 1.5 nm between corresponding intensity maxima in successive scans. This value is sufficient for structural studies of regular biological material to test the performance of STEM over high resolution CTEM.

N and P on-line meters: requirements, maintenance and stability

1996 ◽  
Vol 33 (1) ◽  
pp. 147-157 ◽  
Author(s):  
Henrik A. Thomsen ◽  
Kenneth Kisbye
Keyword(s):  
Sample Preparation ◽  
Response Time ◽  
Ammonium Nitrate ◽  
State Of The Art ◽  
List Price ◽  
User Friendliness ◽  
On Line ◽  
The Cost ◽  
Preparation Design

State-of-the-art on-line meters for determination of ammonium, nitrate and phosphate are presented. The on-line meters employ different measuring principles and are available in many different designs differing with respect to size, calibration and cleaning principle, user-friendliness, response time, reagent and sample consumption. A study of Danish experiences on several plants has been conducted. The list price of an on-line meter is between USD 8000 and USD 35,000. To this should be added the cost of sample preparation, design, installation and running-in. The yearly operating for one meter are in the range of USD 200-2500 and the manpower consumption is in the range of 1-5 hours/month. The accuracy obtained is only slightly smaller than the accuracy on collaborative laboratory analyses, which is sufficient for most control purposes.

scholarly journals Employing multiple synchronous outcome samples per subject to improve study efficiency

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Roger P. A’Hern
Keyword(s):  
Primary Outcome ◽  
Sampling Error ◽  
Optimum Number ◽  
Intra Class Correlation ◽  
Additional Information ◽  
True Value ◽  
The Difference ◽  
Highly Correlated ◽  
The Cost ◽  
Multiple Samples

Abstract Background Accuracy can be improved by taking multiple synchronous samples from each subject in a study to estimate the endpoint of interest if sample values are not highly correlated. If feasible, it is useful to assess the value of this cluster approach when planning studies. Multiple assessments may be the only method to increase power to an acceptable level if the number of subjects is limited. Methods The main aim is to estimate the difference in outcome between groups of subjects by taking one or more synchronous primary outcome samples or measurements. A summary statistic from multiple samples per subject will typically have a lower sampling error. The number of subjects can be balanced against the number of synchronous samples to minimize the sampling error, subject to design constraints. This approach can include estimating the optimum number of samples given the cost per subject and the cost per sample. Results The accuracy improvement achieved by taking multiple samples depends on the intra-class correlation (ICC). The lower the ICC, the greater the benefit that can accrue. If the ICC is high, then a second sample will provide little additional information about the subject’s true value. If the ICC is very low, adding a sample can be equivalent to adding an extra subject. Benefits of multiple samples include the ability to reduce the number of subjects in a study and increase both the power and the available alpha. If, for example, the ICC is 35%, adding a second measurement can be equivalent to adding 48% more subjects to a single measurement study. Conclusion A study’s design can sometimes be improved by taking multiple synchronous samples. It is useful to evaluate this strategy as an extension of a single sample design. An Excel workbook is provided to allow researchers to explore the most appropriate number of samples to take in a given setting.

scholarly journals Reproductive and cost assessment of a seasonal breeding program with Bos indicus in tropical Mexico

2021 ◽  
Vol 26 (2) ◽  
pp. e2130
Author(s):  
José Francisco Martínez-Albarrán ◽  
Carlos Salvador Galina-Hidalgo ◽  
Ivette Rubio-Gutiérrez ◽  
Wendy Leticia Balam-Villarreal ◽  
Manuel D. Corro-Morales
Keyword(s):  
Pregnancy Rate ◽  
Breeding Season ◽  
Phase 1 ◽  
Bos Indicus ◽  
Fixed Time ◽  
Breeding Program ◽  
Tropical Region ◽  
Two Phases ◽  
One Year ◽  
The Cost

Objective. To compare the reproductive performance of postpartum and open Bos indicus cows and to study the cost effectiveness of retaining non-pregnant animals after a short breeding season in tropical region of Mexico. Material and Methods. A total of 128 Bos indicus were included, 87 postpartum cows (PP) with ≤90 days after calving and 41 open cows (OC) with >90 days open. The study was divided into three phases: 1) Estrus synchronization followed by FTAI (day 0-10), 2) Estrus detection and AI (day 11-45) and 3) Natural mating (day 46-90). For the first phase, all animals were synchronized and AI at fixed time (day 10). Cows displaying overt signs of estrus (day 11-45) were AI. Open cows during the previous two phases were exposed to the bull. Results. Pregnancy in phase 1 was different (p<0.01) for PP and OC groups, 58.6% and 34.1%, respectively. Overall pregnancy percentage over the second service was 42.5% (p>0.05). No differences (p>0.05) were observed at phase 3, average 44.2%. By the end of the breeding season, the cost of OC, was 3 times more than PP cows. Conclusions. Pregnancy rate at first phase was higher in PP cows than OC cows. At the end of breeding season, a pregnancy rate of 80% was found. Incorporation of open cows from previous breeding season was more expensive than PP cows in all phases of the breeding program. Retaining an open cow for rebreeding one year or more could not be economically feasible.

Two-Step Process Identification With Correlation Analysis and Least-Squares Parameter Estimation

1974 ◽  
Vol 96 (4) ◽  
pp. 426-432 ◽  
Author(s):  
R. Isermann ◽  
U. Bauer
Keyword(s):  
Correlation Analysis ◽  
Process Model ◽  
A Priori ◽  
Industrial Process ◽  
Computation Time ◽  
Small Computer ◽  
Nonparametric Model ◽  
Identification Method ◽  
On Line ◽  
Process Computer

An identification method is described which first identifies a linear nonparametric model (crosscorrelation function, impulse response) by correlation analysis and then estimates the parameters of a parametric model (discrete transfer function) and also includes a method for the detection of the model order and the time delay. The performance, the computational expense and the overall reliability of this method is compared with five other identification methods. This two-step identification method, which can be applied off-line or on-line, is especially suited to identification by process computers, since it has the properties: Little a priori knowledge about the structure of the process model; very short computation time; small computer storage; no initial values of matrices and parameters are necessary and no divergence is possible for the on-line version. Results of an on-line identification of an industrial process with a process computer are shown.

Metabolic rates of freely diving Weddell seals: correlations with oxygen stores, swim velocity and diving duration

1992 ◽  
Vol 165 (1) ◽  
pp. 181-194 ◽  
Author(s):  
M. A. Castellini ◽  
G. L. Kooyman ◽  
P. J. Ponganis
Keyword(s):  
Oxygen Consumption ◽  
Dive Duration ◽  
Metabolic Rates ◽  
Theoretical Limit ◽  
Weddell Seals ◽  
Consumption Values ◽  
Aerobic Dive Limit ◽  
Significant Difference ◽  
On Line ◽  
The Cost

The metabolic rates of freely diving Weddell seals were measured using modern methods of on-line computer analysis coupled to oxygen consumption instrumentation. Oxygen consumption values were collected during sleep, resting periods while awake and during diving periods with the seals breathing at the surface of the water in an experimental sea-ice hole in Antarctica. Oxygen consumption during diving was not elevated over resting values but was statistically about 1.5 times greater than sleeping values. The metabolic rate of diving declined with increasing dive duration, but there was no significant difference between resting rates and rates in dives lasting up to 82 min. Swimming speed, measured with a microprocessor velocity recorder, was constant in each animal. Calculations of the aerobic dive limit of these seals were made from the oxygen consumption values and demonstrated that most dives were within this theoretical limit. The results indicate that the cost of diving is remarkably low in Weddell seals relative to other diving mammals and birds.

E-Impresa

2010 ◽  
pp. 470-485
Author(s):  
Habib Sedehi
Keyword(s):  
Sustainable Growth ◽  
Strategic Decisions ◽  
Whole Process ◽  
On Line ◽  
Business Game ◽  
Break Even Point ◽  
The Cost ◽  
The Web

Electronic commerce, marketing on line, and network economy are today’s keywords of (possible) success. But how many managers effectively know about the cost and benefits of starting to sell their products and services through the Web? How much they should invest at the beginning and how long does it takes to have a break-even point of their investment? In order to give support for better understanding the process of the Web marketing and to have more elements to decide to “dive” or not in this virtual world a System Dynamics (SD) model (Forrester J.W. 1961, 1971, 1980), has been developed. The model has the aim to support strategic decisions for SME involvement in e-Commerce, pointed out to guarantee sustainable growth and medium-long term success. The project e-Impresa1 analyses the whole process of the investment in building and maintaining a web site, taking into account the main variables of E-commerce. Through a case study, a SD business game model has been developed. The model gives the opportunity to users to evaluate different what-if analysis through the simulation period time (2 years) at each model step time (4 weeks). This chapter will explain the overall architecture of the model and will present some results of use of the model in different conditions.

The Role of Information Technology in Quality Education

2000 ◽  
pp. 177-197 ◽  
Author(s):  
G. David Garson
Keyword(s):  
Information Technology ◽  
Instructional Strategy ◽  
Large Scale ◽  
University Teaching ◽  
Programmed Learning ◽  
Face To Face ◽  
On Line ◽  
Future Of Education ◽  
The Cost ◽  
The University

Discusses the rise of information technology functions in higher education and the related advent of quality standards for on-line education. The limited nature of existing guidelines is emphasized, particularly in the dimension of establishing authentic relationships, empowerment of faculty and students, and the inculcation of critical thinking. The Borkian vision of the future of education is summarized and contrasted with the limits if not failure of the legacies of past large-scale educational investments in programmed learning and in computer simulation. The drift toward mandated standards in on-line/distance education is discussed as well as the tension of this with empowerment concepts. Further contrast is drawn between the competing models of the university as “community of scholars” and as “marketplace of consumers.” Cost-cutting motives for on-line course delivery are explored in some detail, raising issues about radical proposals to restructure university teaching functions. A hybrid model, involving both computer-mediated and face-to-face methods, is seen as the superior instructional strategy, but the cost of this model raises the danger that a two-tier educational system will emerge – a more expensive upper tier with sound traditional education supplemented with the benefits of electronic media, and a cheaper, inferior tier dispensing programmed training to meet objectives far narrower than the traditional goals of liberal education.

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