Morphological evidence for the antiatherogenic effect of scoparone in hyperlipidaemic diabetic rabbits

Recent ultrastructural studies on endometrial tissues from women wearing copper, wire intrauterine devices have disclosed morphological evidence of impaired glycogen degradation and secretion resulting in interference with the viability of blastocysts. Reduced microapocrine secretion observed with the scanning electron microscope supports this (1). In addition, organelle modifications have been observed in the epithelial cells of these women. The changes are seen in biopsies taken in the proliferative phase of the cycle and consist of mitochondrial vacuolation and myelin figure formation. These modifications disappear in the secretory phase and therefore have been regarded as reversible (2).The aim of the present studies was to investigate surface epithelial changes as well as organelle modifications in relation to the site of contact with an IUD that releases greater amounts of copper. Endometrial tissue was obtained from the uterine cavity of four young women wearing TCu-380-A intrauterine contraceptive devices for 4-6 weeks.

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Ultrastructural Study of a differentiating human colon carcinoma cell line

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100158583 ◽

1990 ◽

Vol 48 (3) ◽

pp. 208-209

Author(s):

Sylvie Polak-Charcon ◽

Mehrdad Hekmati ◽

Yehuda Ben Shaul

Keyword(s):

Cell Line ◽

Morphological Changes ◽

Secretory Granules ◽

Human Colon ◽

Cell Types ◽

Culture Conditions ◽

Morphological Evidence ◽

Human Colon Carcinoma Cell ◽

Colon Cell

The epithelium of normal human colon mucosa “in vivo” exhibits a gradual pattern of differentiation as undifferentiated stem cells from the base of the crypt of “lieberkuhn” rapidly divide, differentiate and migrate toward the free surface. The major differentiated cell type of the intestine observed are: absorptive cells displaying brush border, goblet cells containing mucous granules, Paneth and endocrine cells containing dense secretory granules. These different cell types are also found in the intestine of the 13-14 week old embryo.We present here morphological evidence showing that HT29, an adenocarcinoma of the human colon cell line, can differentiate into various cell types by changing the growth and culture conditions and mimic morphological changes found during development of the intestine in the human embryo.HT29 cells grown in tissue-culture dishes in DMEM and 10% FCS form at late confluence a multilayer of morphologically undifferentiated cell culture covered with irregular microvilli, and devoid of tight junctions (Figs 1-3).

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Ultrastructural evidence of transplacental transport of immunoglobulin G in bitches

Reproduction ◽

10.1530/reprod/118.2.315 ◽

2000 ◽

pp. 315-326 ◽

Cited By ~ 6

Author(s):

MH Stoffel ◽

AE Friess ◽

SH Hartmann

Keyword(s):

Mammary Gland ◽

Basement Membrane ◽

Immunoglobulin G ◽

Passive Immunity ◽

Morphological Evidence ◽

Membrane Material ◽

Ultrastructural Evidence ◽

Cytotrophoblast Cells ◽

Fetal Vessels

In dogs, passive immunity is conferred to fetuses and neonates by the transfer of maternal immunoglobulin G through the placenta during the last trimester of pregnancy and via the mammary gland after parturition, respectively. However, morphological evidence of transplacental transport is still lacking. The aim of the present study was to localize maternal immunoglobulin G in the labyrinthine zone and in the haemophagous zone of the canine placenta by means of immunohistochemistry and immunocytochemistry. In the labyrinthine zone, immunoglobulin G was detected in all the layers of the materno-fetal barrier including the fetal capillaries. Immunoreactivity was particularly prominent in maternal basement membrane material as well as in the syncytiotrophoblast. However, this evidence of transplacental transport of immunoglobulin G originated from a limited number of unevenly distributed maternal vessels only. In the cytotrophoblast of the haemophagous zone, immunoglobulin G was localized to phagolysosomes at various stages but was never detected within fetal vessels. The results indicate that maternal immunoglobulin G is degraded in cytotrophoblast cells of the hemophagous zone and, therefore, that transplacental transport is restricted to a subpopulation of maternal vessels in the labyrinthine zone.

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Diversification of the North American Doellingeria-Eucephalus Clade (Astereae: Asteraceae) Inferred from Molecular and Morphological Evidence

Systematic Botany ◽

10.1600/036364419x15710776741477 ◽

2019 ◽

Vol 44 (4) ◽

pp. 930-942

Author(s):

Geraldine A. Allen ◽

Luc Brouillet ◽

John C. Semple ◽

Heidi J. Guest ◽

Robert Underhill

Keyword(s):

North American ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Sister Group ◽

Morphological Evidence ◽

South American ◽

New Combinations ◽

The North ◽

Hybridization Event ◽

Ancient Hybridization

Abstract—Doellingeria and Eucephalus form the earliest-diverging clade of the North American Astereae lineage. Phylogenetic analyses of both nuclear and plastid sequence data show that the Doellingeria-Eucephalus clade consists of two main subclades that differ from current circumscriptions of the two genera. Doellingeria is the sister group to E. elegans, and the Doellingeria + E. elegans subclade in turn is sister to the subclade containing all remaining species of Eucephalus. In the plastid phylogeny, the two subclades are deeply divergent, a pattern that is consistent with an ancient hybridization event involving ancestral species of the Doellingeria-Eucephalus clade and an ancestral taxon of a related North American or South American group. Divergence of the two Doellingeria-Eucephalus subclades may have occurred in association with northward migration from South American ancestors. We combine these two genera under the older of the two names, Doellingeria, and propose 12 new combinations (10 species and two varieties) for all species of Eucephalus.

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Anti-Diabetic Effect of a Flavonoid and Sitosterol - Rich Aqueous Extract ofPleurotus tuberregiumSclerotia in Alloxan-Induced Diabetic Rabbits

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530319666190206213843 ◽

2019 ◽

Vol 19 (8) ◽

pp. 1148-1156 ◽

Cited By ~ 3

Author(s):

Ifeanacho Mercy Onuekwuzu ◽

Ikewuchi Catherine Chidinma ◽

Ikewuchi Jude Chigozie

Keyword(s):

Diabetes Mellitus ◽

Blood Glucose ◽

Aqueous Extract ◽

Risk Index ◽

Fasting Blood Glucose ◽

Hdl Cholesterol ◽

The Body ◽

Atherogenic Index ◽

Atherogenic Indices ◽

Diabetic Rabbits

Objective:Traditionally prepared infusions and decoctions are commonly used in the management of diabetes mellitus, in southern Nigeria; one of such is the aqueous extract of the sclerotia of Pleurotus tuberregium (“usu” milk). In this study, the effects of the extract on the body weights, tissue/ organ weights, fasting blood glucose, blood/plasma lipid profiles and atherogenic indices were investigated in normal and alloxan-induced diabetic rabbits.Methods:Diabetes mellitus was induced by the injection of alloxan (120 mg/kg body weight) via the marginal ear vein. The extract was administered orally at 100, 200 and 300 mg/kg to normal and diabetic rabbits; while metformin was administered at 50 mg/kg. The crude extract was analyzed by gas chromatography, coupled to flame ionization detector.Results:Thirty-one known flavonoids were detected, consisting mainly of isoquercetin (28.5%), luteolin (24.3%), quercetin (18.8%) and kaempferol (11.3%). Sitosterol (82.0%) and stigmasterol (12.5%) were the most abundant of the seven phytosterols detected. Compared to the diabetic control, the treatment significantly (p<0.05) lowered the weights of the kidney and liver, as well as the levels of blood glucose and triglyceride, plasma VLDL, LDL and non-HDL cholesterol, atherogenic index of plasma, cardiac risk ratio, atherogenic coefficient and Castelli’s risk index II. It, however, significantly (p<0.05) increased plasma HDL cholesterol, without significantly affecting blood total cholesterol levels.Conclusion:This study showed that the extract was hypoglycemic, and improved lipid profile and atherogenic indices, thus highlighting its cardioprotective potential, thereby supporting its use in the management of diabetes mellitus.

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Morphological evolution of supermassive black hole merger hosts and multimessenger signatures

Monthly Notices of the Royal Astronomical Society ◽

10.1093/mnras/stab721 ◽

2021 ◽

Vol 503 (3) ◽

pp. 3629-3642

Author(s):

Colin DeGraf ◽

Debora Sijacki ◽

Tiziana Di Matteo ◽

Kelly Holley-Bockelmann ◽

Greg Snyder ◽

...

Keyword(s):

Black Holes ◽

Black Hole ◽

Star Formation ◽

Morphological Evolution ◽

Full Range ◽

High Mass ◽

Morphological Evidence ◽

Galaxy Mergers ◽

Host Galaxies ◽

Supermassive Black Hole

ABSTRACT With projects such as Laser Interferometer Space Antenna (LISA) and Pulsar Timing Arrays (PTAs) expected to detect gravitational waves from supermassive black hole mergers in the near future, it is key that we understand what we expect those detections to be, and maximize what we can learn from them. To address this, we study the mergers of supermassive black holes in the Illustris simulation, the overall rate of mergers, and the correlation between merging black holes and their host galaxies. We find these mergers occur in typical galaxies along the MBH−M* relation, and that between LISA and PTAs we expect to probe the full range of galaxy masses. As galaxy mergers can trigger star formation, we find that galaxies hosting low-mass black hole mergers tend to show a slight increase in star formation rates compared to a mass-matched sample. However, high-mass merger hosts have typical star formation rates, due to a combination of low gas fractions and powerful active galactic nucleus feedback. Although minor black hole mergers do not correlate with disturbed morphologies, major mergers (especially at high-masses) tend to show morphological evidence of recent galaxy mergers which survive for ∼500 Myr. This is on the same scale as the infall/hardening time of merging black holes, suggesting that electromagnetic follow-ups to gravitational wave signals may not be able to observe this correlation. We further find that incorporating a realistic time-scale delay for the black hole mergers could shift the merger distribution towards higher masses, decreasing the rate of LISA detections while increasing the rate of PTA detections.

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In vivo study of the angiogenesis potential of bone marrow-derived mesenchymal stem cell aggregates in their niche like environment

The International Journal of Artificial Organs ◽

10.1177/03913988211025538 ◽

2021 ◽

pp. 039139882110255

Author(s):

Sara Anajafi ◽

Azam Ranjbar ◽

Monireh Torabi-Rahvar ◽

Naser Ahmadbeigi

Keyword(s):

Tissue Engineering ◽

Bone Marrow ◽

Stem Cell ◽

Mesenchymal Stem Cell ◽

Cultured Cells ◽

Morphological Evidence ◽

Cell Aggregates ◽

Plla Scaffold ◽

Significant Difference

Background: Sufficient blood vessel formation in bioengineered tissues is essential in order to keep the viability of the organs. Impaired development of blood vasculatures results in failure of the implanted tissue. The cellular source which is seeded in the scaffold is one of the crucial factors involved in tissue engineering methods. Materials and methods: Considering the notable competence of Bone Marrow derived Mesenchymal Stem Cell aggregates for tissue engineering purposes, in this study BM-aggregates and expanded BM-MSCs were applied without any inductive agent or co-cultured cells, in order to investigate their own angiogenesis potency in vivo. BM-aggregates and BM-MSC were seeded in Poly-L Lactic acid (PLLA) scaffold and implanted in the peritoneal cavity of mice. Result: Immunohistochemistry results indicated that there was a significant difference ( p < 0.050) in CD31+ cells between PLLA scaffolds contained cultured BM-MSC; PLLA scaffolds contained BM-aggregates and empty PLLA. According to morphological evidence, obvious connections with recipient vasculature and acceptable integration with surroundings were established in MSC and aggregate-seeded scaffolds. Conclusion: Our findings revealed cultured BM-MSC and BM-aggregates, capacity in order to develop numerous connections between PLLA scaffold and recipient’s vasculature which is crucial to the survival of tissues, and considerable tendency to develop constructs containing CD31+ endothelial cells which can contribute in vessel’s tube formation.

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Analysis of Lipid Peroxidation by UPLC-MS/MS and Retinoprotective Effects of the Natural Polyphenol Pterostilbene

Antioxidants ◽

10.3390/antiox10020168 ◽

2021 ◽

Vol 10 (2) ◽

pp. 168

Author(s):

Isabel Torres-Cuevas ◽

Iván Millán ◽

Miguel Asensi ◽

Máximo Vento ◽

Camille Oger ◽

...

Keyword(s):

Lipid Peroxidation ◽

Diabetic Retinopathy ◽

Redox Homeostasis ◽

Ultra Performance Liquid Chromatography ◽

Protective Effects ◽

Diabetic Retina ◽

Key Factor ◽

Adrenic Acid ◽

Diabetic Rabbits ◽

High Level

The loss of redox homeostasis induced by hyperglycemia is an early sign and key factor in the development of diabetic retinopathy. Due to the high level of long-chain polyunsaturated fatty acids, diabetic retina is highly susceptible to lipid peroxidation, source of pathophysiological alterations in diabetic retinopathy. Previous studies have shown that pterostilbene, a natural antioxidant polyphenol, is an effective therapy against diabetic retinopathy development, although its protective effects on lipid peroxidation are not well known. Plasma, urine and retinas from diabetic rabbits, control and diabetic rabbits treated daily with pterostilbene were analyzed. Lipid peroxidation was evaluated through the determination of derivatives from arachidonic, adrenic and docosahexaenoic acids by ultra-performance liquid chromatography coupled with tandem mass spectrometry. Diabetes increased lipid peroxidation in retina, plasma and urine samples and pterostilbene treatment restored control values, showing its ability to prevent early and main alterations in the development of diabetic retinopathy. Through our study, we are able to propose the use of a derivative of adrenic acid, 17(RS)-10-epi-SC-Δ15-11-dihomo-IsoF, for the first time, as a suitable biomarker of diabetic retinopathy in plasmas or urine.

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