scholarly journals Immunomodulatory Activity ofChlorophytum borivilianumSant. F

2007 ◽  
Vol 4 (4) ◽  
pp. 419-423 ◽  
Author(s):  
Mayank Thakur ◽  
Shilpi Bhargava ◽  
V. K. Dixit
Keyword(s):  
Ethanolic Extract ◽  
Delayed Type Hypersensitivity ◽  
Immunomodulatory Activity ◽  
Traditional Use ◽  
Activity Effect ◽  
Carbon Clearance ◽  
Traditional Indian Medicine ◽  
Indian Medicine ◽  
Delayed Type Hypersensitivity Response

Chlorophytum borivilianumSantapau & Fernandes (Liliaceae) is a very popular herb in traditional Indian medicine and constitute a group of herbs used as ‘Rasayan’ or adaptogen. Ethanolic extract of the roots and its sapogenin were evaluated for their immunomodulatory activity. Effect of azathioprine-induced myelosuppresion and administration of extracts on hematological and serological parameters was determined. Administration of extracts greatly improved survival againstCandida albicansinfection. An increase in delayed-type hypersensitivity response (DTH), % neutrophil adhesion andin vivophagocytosis by carbon clearance method was observed after treatment with extracts. Immunostimulant activity of ethanolic extract was more pronounced as compared to sapogenins. The results, thus justifies the traditional use ofC. borivilianumas a rasayana drug.

scholarly journals EVALUATION OF IN VIVO IMMUNOMODULATORY ACTIVITY OF AQUEOUS AND ETHANOLIC EXTRACT OF CAPPARIS MOONII

2020 ◽  
pp. 63-66
Author(s):  
VANITA G KANASE ◽  
SHRUTI SHETTIGAR
Keyword(s):  
Antibody Titer ◽  
Ethanolic Extract ◽  
Experimental Methods ◽  
Delayed Type Hypersensitivity ◽  
Phagocytic Index ◽  
Immunomodulatory Activity ◽  
Dried Fruits ◽  
Carbon Clearance ◽  
Stimulation Of

Objective: The present study was intended to evaluate the in vivo immunomodulatory activity of aqueous and ethanolic extract of dried fruits of Capparis moonii Wight. Methods: The effect of both the extracts was evaluated by carbon clearance assay in mice, and they showed a significant increase in the phagocytic index at 50, 100, and 200 mg/kg dose. The effect of the extracts on delayed-type hypersensitivity (DTH) and antibody titer assay was evaluated in ovalbumin immunized mice. Results: Both extracts showed a significant effect on DTH response and stimulation of antibody titer at 200 mg/kg dose. The effects of the extracts in cyclophosphamide-induced myelosuppression mice were also statistically significant. Conclusion: The studies demonstrated specific and non-specific immunostimulating properties of both the ethanol and aqueous extract of C. moonii fruits in various in vivo experimental methods suggesting its therapeutic usefulness in immunocompromised conditions.

In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract

Planta Medica ◽  
2020 ◽  
Author(s):  
Sérgio Ricardo Teixeira Daltro ◽  
Ivanilson Pimenta Santos ◽  
Paula Ladeia Barros ◽  
Diogo Rodrigo Magalhães Moreira ◽  
Therezinha Coelho Barbosa Tomassini ◽  
...  
Keyword(s):  
Cytokine Production ◽  
Ethanolic Extract ◽  
Delayed Type Hypersensitivity ◽  
Immunomodulatory Activity ◽  
Hypersensitivity Response ◽  
Immunomodulatory Drugs ◽  
Activated Macrophages ◽  
Physalis Angulata ◽  
Delayed Type Hypersensitivity Response

AbstractThe need for new immunomodulatory drugs is due to the side effects associated with the prolonged use of the currently used immunomodulatory drugs. In this context, the present work aimed to investigate the immunomodulatory effect of an ethanolic concentrated extract from Physalis angulata. The cytotoxicity of samples was determined using peritoneal macrophages though the Alamar Blue assay. The immunomodulatory activity of the ethanolic extract from P. angulata on activated macrophages was determined by measurement of nitrite and cytokine production. The immunosuppressive effects of the ethanolic extract from P. angulata was evaluated on lymphocyte proliferation and cytokine production. The effects of the extract on cell cycle progression and cell death on lymphocytes were evaluated by flow cytometry. Lastly, the ethanolic extract from P. angulata was tested in vivo in toxicological tests and in models of peritonitis and delayed-type hypersensitivity response. The ethanolic extract from P. angulata decreased nitrite, interleukin-6, interleukin-12, and TNF-α production by activated macrophages without affecting the cell viability. In addition, the ethanolic extract from P. angulata inhibited lymphoproliferation and the secretion of interleukin-2, interleukin-6, and IFN-γ, and increased interleukin-4 secretion by activated splenocytes. Flow cytometry analysis in lymphocyte cultures showed that treatment with the ethanolic extract from P. angulata induces cell cycle arrest in the G1 phase followed by cell death by apoptosis. Moreover, mice treated with the extract from P. angulata at 100 or 200 mg/kg did not show signs of toxicity or alterations in serum components. Finally, the ethanolic extract from P. angulata significantly reduced neutrophil migration and reduced paw edema in bovine serum albumin-induced the delayed-type hypersensitivity response model. Our results demonstrate the potential of the ethanolic extract of P. angulata as an alternative for the treatment of immune-inflammatory diseases.

Immunomodulatory activity of Alcoholic extracts of Tinospora cordifolia Stem

2021 ◽  
pp. 73-77
Author(s):  
Sanjiv Kumar Biradar ◽  
Chandra Kishore Tyagi
Keyword(s):  
Experimental Models ◽  
Tinospora Cordifolia ◽  
Delayed Type Hypersensitivity ◽  
Phagocytic Index ◽  
Immunomodulatory Activity ◽  
Neutrophil Adhesion ◽  
Cell Mediated Immunity ◽  
Medicinal Value ◽  
Carbon Clearance

Tinospora cordifolia is a plant well known for its medicinal value in Indian ayurveda and Indian traditional medicine system. However, to prove its efficiency for the clinical utilization, more experimental data will be beneficial. In the present investigation, evaluated the immunomodulatory activity of the alcoholic extracts of Tinospora cordifolia stem on various in-vivo experimental models such as neutrophil adhesion test, phagocytic index by carbon clearance test, Hemagglutinating antibody (HA) titre and delayed type hypersensitivity (DTH) responses. The evaluation of immunomodulatory potential by oral administration of alcoholic stem extracts (50, 100, 200 and 300mg/kg b.w, p.o) evoked a significant increase in percent neutrophil adhesion to nylon fibers as well as a dose dependent increased in antibody titre values, and potentiated delayed type hypersensitivity reaction induced by sheep red blood cells and significant response towards phagocytosis in carbon clearance assay (*p<0.05, *p<0.01, ***p<0.001). This may be due to concentrations of active phytochemicals present in particular plant extract. Hence, it was concluded that the plant extracts increased humoral as well as cell mediated immunity.

scholarly journals EVALUATION OF IN VIVO IMMUNOMODULATORY ACTIVITY OF AQUEOUS AND ETHANOLIC EXTRACT OF EULOPHIA NUDA L.

2018 ◽  
Vol 11 (12) ◽  
pp. 374
Author(s):  
Vanita Kanase ◽  
Diptesh T Patil
Keyword(s):  
Cytochromes P450 ◽  
Combined Treatment ◽  
Serum Antibody ◽  
Ethanolic Extract ◽  
Delayed Type Hypersensitivity ◽  
Control Group ◽  
Immunomodulatory Activity ◽  
Serum Antibody Titer ◽  
Stimulation Of

Objective: The aim of this study was to evaluate the in vivo immunomodulatory activity of an aqueous and ethanolic extract of dried tubers of Eulophia nuda (ENA and ENE).Methods: Effect of both the extracts was evaluated on delayed-type hypersensitivity (DTH) response, serum antibody response, and cyclophosphamide-induced myelosuppression in Swiss albino mice.Results: The extracts showed stimulation of DTH reaction in mice in response to T-cell dependent antigen by both ENE () and ENA (); significant increase in serum antibody titer at of ENA (768.01) and ENE (768.33) extract at 200 mg/kg, compared to control group (213.33); Combined treatment of ENA+Cytochromes P450 [CYP]-25 mg/kg and ENE+CYP-25mg/kg (50, 100, and 200 mg/kg) doses of ENA and ENE each with 25 mg/kg resulted in restoration of bone marrow activity as compared with CYP treatment alone.Conclusion: Both specific and non-specific immunostimulating properties of the ENE and ENA tubers in in vivo experimental methods suggest its therapeutic usefulness in immunocompromised conditions.

scholarly journals Hepatoprotective activity of the ethanolic extract of Morus indica roots from Indian Bodo tribes

2022 ◽  
Vol 4 (2) ◽  
Author(s):  
Hankhray Boro ◽  
Talambedu Usha ◽  
Dinesh Babu ◽  
Prakashmurthy Chandana ◽  
Arvind Kumar Goyal ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Active Components ◽  
Traditional Use ◽  
Hematological Indices ◽  
Pharmacological Mechanism ◽  
Hepatic Lipid Peroxidation

AbstractThe roots of Morus species are well described in the Pharmacopoeia of the People's Republic of China (ChP) for its traditional use in treating liver fibrosis due to its hepatoprotective property. However, little is known about the hepatoprotective effect of the roots of Morus indica L. (RoMi), and the pharmacological mechanism(s) are uncertain due to its intricacy. Therefore, this study evaluates the hepatoprotective activity of the ethanolic extract of RoMi (eRoMi) against the CCl4-induced in-vivo animal model at different dosages (100 and 200 mg/kg BW) in comparison with silymarin as a positive control. The hepatoprotective activity of eRoMi was evaluated by measuring the levels of serum biomarkers, hepatic antioxidant enzymes and was verified by histological studies. Interestingly, 1,2-bis(trimethylsilyl) benzene, 1,4-phenylenebis (trimethylsilane), 2,4,6-cycloheptatriene-1-one, 3,5-bis-trimethylsilyl and α-amyrin were the active components found in eRoMi as detected by GC–MS. Oral administration of eRoMi (200 mg/kg BW) to rats significantly protected serum biochemical parameters (increased ALT, AST, LDH, bilirubin and GGT as well as depletion of antioxidant enzymes and hepatic GSH) and elevation in hepatic lipid peroxidation as compared to CCl4-treated rats. The hematological indices such as erythrocytes, hemoglobin, monocytes and lymphocytes were also normal in eRoMi-treated rats. The histopathological evaluation indicated a significant restoration of liver structure as compared to silymarin. This study is the first scientific validation for the traditional use of eRoMi to understand its hepatoprotective activity.

scholarly journals EVALUATION OF IN VITRO IMMUNOMODULATORY ACTIVITY OF AQUEOUS AND ETHANOLIC EXTRACT OF EULOPHIA NUDA LINDL

2018 ◽  
Vol 11 (11) ◽  
pp. 352 ◽  
Author(s):  
Vanita Kanase ◽  
Diptesh T Patil
Keyword(s):  
Lysosomal Enzyme ◽  
Stimulation Index ◽  
Ethanolic Extract ◽  
In Vivo Studies ◽  
Phagocytic Index ◽  
Immunomodulatory Activity ◽  
Myeloperoxidase Activity ◽  
Enzyme Release

Objective: The aim of this study was to evaluate the in vitro immunomodulatory activity of aqueous and ethanolic extract of dried tubers of Eulophia nuda.Methods: Effect of both the extracts was evaluated at various concentrations (832–6.5 μg/ml) for secretion of mediators such as nitric oxide (NO), superoxide, lysosomal enzyme, and myeloperoxidase activity of isolated murine peritoneal macrophages.Results: The extracts showed stimulation of NO, statistically significant at 832 μg/ml (SI 1.739) for ENA and at 832 μg/ml (stimulation index [SI] 1.662) for ENE; significant stimulation on lysosomal enzyme release for ENA at 832 μg/ml (SI 1.404) and ENE at 832 μg/ml (SI 1.513); myeloperoxidase activity was statistically significant for ENA at 832 μg/ml (SI 1.728) and ENE at 832 μg/ml (SI 1.770).Conclusion: In vitro phagocytic index showed significant results and thus proving the need for confirmation through in vivo studies.

IN-VIVO IMMUNOMODULATORY ACTIVITY OF ETHANOLIC EXTRACTS OF AERIAL PARTS OF PHYLLANTHUS AMARUS PLANT IN CYCLOPHOSPHAMIDE INDUCED MICE

2021 ◽  
Vol 10 (1) ◽  
pp. 11-15
Author(s):  
Veershetty Hachhe ◽  
Siddaiah M
Keyword(s):  
Ethanolic Extract ◽  
Treated Group ◽  
Immunomodulatory Activity ◽  
Phyllanthus Amarus ◽  
Group Iii ◽  
Platelet Counts ◽  
Aerial Parts ◽  
Ethanolic Extracts ◽  
Respective Treatment

Objective: To evaluate the immunomodulatory activity of ethanolic extract of aerial parts of phyllanthus amarus plant in cyclophosphamide induced mice. Materials and Method: To evaluate the immunomodulatory activity of ethanolic extracts of Aerial parts of phyllanthus amarus plant. on cyclophosphamide induced immunosuppression in mice. ethanolic extracts of aerial parts of phyllanthus amarus plant,  (500mg/kg p.o.) were administered 13 days to albino mice and cyclophosphamide (30mg/kg i.p.) was administered on 11th,12th and 13th days 1 hour after the administration of the respective treatment. On the 14th day blood was collected by retro orbital puncture and the activity was evaluated by determining the RBC, Hb%, Platelet, total WBC and differential counts. Ethanolic extracts of aerial parts phyllantus amarus plant. showed very significant (p<0.001) counteracting effect to cyclophosphamide induced reduction in total WBC, DLC and platelet counts & significant (P<0.01) effect to that of reduction in RBC counts and Hb %. The significant inmmunostimulant effect of the ethanolic extracts of aerial parts of phyllanthus amarus plant on cyclophosphamide induced myelosuppression may be attributed towards the collective presence of saponins, sterols and tannins in the extracts, which suggest the immunomodulatory activities of the ethanolic extracts of phyllanthusamarus plant, leaves,stem and fruits. Results: The results of the study revealed the dose dependent effect of theextracts in counteracting the myelosuppression induced by cyclophosphamide as inducated by increase in RBC total WBC platelet counts, Hb% and DLC in the extract treated group (III &IV), when compared to cyclophoshamide treated group (Group II). Conclusion: Ethanolic extract of phyllanthus amarus would contribute for significant immunostimulating activity of In-Vivo Keywords: Phyllanthus amarus, Cyclophosphamide, Immunomodulatory, Myelosuppression

scholarly journals Nanotized Curcumin and Miltefosine, a Potential Combination for Treatment of Experimental Visceral Leishmaniasis

2016 ◽  
Vol 61 (3) ◽  
Author(s):  
Brajendra Tiwari ◽  
Richa Pahuja ◽  
Pradeep Kumar ◽  
Srikanta Kumar Rath ◽  
Kailash Chand Gupta ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Leishmania Donovani ◽  
Oral Drug ◽  
Hamster Model ◽  
Content Type ◽  
Leishmanicidal Activity ◽  
Traditional Indian Medicine ◽  
Indian Medicine

ABSTRACT Leishmaniasis chemotherapy remains very challenging due to high cost of the drug and its associated toxicity and drug resistance, which develops over a period of time. Combination therapies (CT) are now in use to treat many diseases, such as cancer and malaria, since it is more effective and affordable than monotherapy. CT are believed to represent a new explorable strategy for leishmaniasis, a neglected tropical disease caused by the obligate intracellular parasite Leishmania. In the present study, we investigated the effect of a combination of a traditional Indian medicine (ayurveda), a natural product curcumin and miltefosine, the only oral drug for visceral leishmaniasis (VL) using a Leishmania donovani-hamster model. We developed an oral nanoparticle-based formulation of curcumin. Nanoformulation of curcumin alone exhibited significant leishmanicidal activity both in vitro and in vivo. In combination with miltefosine, it exhibited a synergistic effect on both promastigotes and amastigotes under in vitro conditions. The combination of these two agents also demonstrated increased in vivo leishmanicidal activity accompanied by increased production of toxic reactive oxygen/nitrogen metabolites and enhanced phagocytic activity. The combination also exhibited increased lymphocyte proliferation. The present study thus establishes the possible use of nanocurcumin as an adjunct to antileishmanial chemotherapy.

scholarly journals Evaluation of the Antioxidant and Anti-diabetic Effect of Mucuna puriens Extract

2019 ◽  
pp. 1-9
Author(s):  
N. Nwaji Njemuwa ◽  
N. Uduagwu Dickson ◽  
A. Elekwa Elizabeth ◽  
R. Mgbenka Uchenna ◽  
C. Njoku Ogbonnaya
Keyword(s):  
Antioxidant Activity ◽  
Linear Regression Analysis ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Good Evidence ◽  
Mucuna Pruriens ◽  
Leaf Extracts ◽  
Traditional Use ◽  
Reference Drug

Aims: To investigate the scientific basis for the anti-diabetic and antioxidant activity of Mucuna pruriens (Fabaceae) leaf ethanolic extract using alloxan-induced diabetic rats, DPPH and ABTS assay. Place and Duration of Study: Department of Chemistry/Biochemistry Federal University Ndufu Alike, Ebonyi State, Nigeria between October 2013 and May 2014. Methodology: The polyphenol content was determined using Folin-Ciocaltu method and their linear relationship with antioxidant activity was evaluated using linear regression analysis. The antioxidant activity was determined using 1, 1-Diphenyl, 2-picrylhydrazyl (DPPH) and (2, 2-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radical assay. The active ingredients in the ethanolic extract were isolated using HPLC method. Also the ant-diabetic activity was determined in vivo using alloxan-induced diabetic wister rats.   Results: Ethanol extract showed the highest phenolic content as well as highest antioxidant activity. A strong relationship was found between phenolic contents and antioxidant activity. The HPLC analysis indicates the presence of gallic acid, caffeic acid, p-coumaric acid, quercetin and (+)-catechin. The ethanolic extract at the concentration of 400 mg/kg significantly (P<0.001) increased the intracellular antioxidant enzymes and reduced the elavated serum lipids and showed more active than the reference drug (metformin). Conclusion: Based on the obtained result, the antioxidant and anti-diabetic activity demonstrated by Mucuna pruriens leaf extracts provide good evidence to support the traditional use of this plant in treatment of diabetics.

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