scholarly journals Serum Procalcitonin Levels Predict Mortality Risk in Patients With Pulmonary Tuberculosis: A Single-Center Prospective Observational Study

2020 ◽  
Vol 222 (10) ◽  
pp. 1651-1654
Author(s):  
Takeshi Osawa ◽  
Masato Watanabe ◽  
Kozo Morimoto ◽  
Masao Okumura ◽  
Takashi Yoshiyama ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Mortality Risk ◽  
Prospective Observational Study ◽  
Bacterial Load ◽  
Serum Levels ◽  
Risk Indicator ◽  
Radiographic Findings ◽  
Tuberculosis Patients ◽  
Antituberculosis Treatment ◽  
Immunodeficiency Virus

Abstract Globally, tuberculosis is the leading infectious cause of death; discovering biomarkers that predict a high mortality risk may improve treatment outcomes. We prospectively enrolled 252 pulmonary tuberculosis patients who were not coinfected with human immunodeficiency virus and initiated antituberculosis treatment, measured serum procalcitonin levels (PCT), and assessed mortality risk. PCT serum levels higher than 0.13 (day 0), 0.05 (day 7), 0.12 (day 14), or 0.06 (day 28) ng/mL predicted nonsurvivors with odds ratios of 7.9, 14.3, 20.0, and 7.3, respectively (P ≤ .005 for all), respectively. Therefore, serum PCT levels are a promising mortality risk indicator for patients with pulmonary tuberculosis. Main Point. For patients with pulmonary tuberculosis, a promising mortality risk indicator is the level of serum procalcitonin, which is weakly associated with sputum bacterial load and independent of radiographic findings.

scholarly journals Oxidative stress biomarkers in pulmonary tuberculosis patients in Gombe, North-eastern Nigeria

2019 ◽  
Vol 10 (6) ◽  
pp. 57-62 ◽  
Author(s):  
Mohammed Haruna Yeldu ◽  
Yakubu Ibrahim ◽  
Shehu Abubakar Akuyam ◽  
Isah Muhammad Danasabe ◽  
Buhari Shehu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pulmonary Tuberculosis ◽  
Total Antioxidant Status ◽  
Serum Levels ◽  
Newly Diagnosed ◽  
Oxidative Stress Biomarkers ◽  
Tuberculosis Patients ◽  
Stress Biomarkers ◽  
North Eastern ◽  
Total Antioxidant

Background: Oxidative stress may play an important role in the pathogenesis of pulmonary tuberculosis (PTB). To our knowledge there is paucity of data on the status of oxidative stress biomarkers among PTB patients in Gombe, North-eastern Nigeria. Our study was designed to evaluate the oxidative stress biomarkers in pulmonary tuberculosis patients in Gombe, North-eastern Nigeria. Aims and Objectives: To determine the serum levels of oxidative stress biomarkers among patients with pulmonary tuberculosis in Gombe metropolis, North-eastern Nigeria and to assess the correlation between the oxidative stress biomarkers in pulmonary tuberculosis patients. Materials and Methods: A cross sectional comparative study was conducted in a tertiary health care facility with 40 pulmonary tuberculosis (PTB) patients on anti-TB drugs treatment (ATT), 40 newly diagnosed PTB patients not yet on anti-TB drugs treatment (ATT-naïve) and 40 age- and sex-marched apparently healthy subjects (controls). Serum total antioxidant status (TAS), total oxidant status (TOS), malondialdehyde (MDA), nitric oxide (NO) and oxidative stress index (OSI) determined using standard techniques. Data was analysed using INSTAT® (Graph Pad Software Inc., La Jolla, CA, USA). Results: Serum levels of TOS, OSI, MDA and NO were significantly (p ˂ 0.001) increased in PTB patients (ATT and ATT-naïve) when compared with healthy individuals. Serum TAS and body mass index (BMI) were significantly (p ˂ 0.001) decreased in PTB patients when compared with healthy individuals. Serum TOS significantly correlated with serum OSI, MDA and NO in ATT-naïve PTB patients. Conclusion: This study observed an increased oxidative stress biomarkers and decreased total antioxidant status in newly diagnosed pulmonary tuberculosis patients and those on treatment. Our findings suggest that antioxidants supplementation and improved nutrition in the management of pulmonary tuberculosis patients may go a long way in preventing the oxidative onslaught and further complications in PTB patients.

scholarly journals Inflammatory and immunogenetic markers in correlation with pulmonary tuberculosis

2013 ◽  
Vol 39 (6) ◽  
pp. 719-727 ◽  
Author(s):  
Beatriz Lima Alezio Muller ◽  
Daniela Maria de Paula Ramalho ◽  
Paula Fernanda Gonçalves dos Santos ◽  
Eliene Denites Duarte Mesquita ◽  
Afranio Lineu Kritski ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Pulmonary Tuberculosis ◽  
Gene Polymorphisms ◽  
Serum Levels ◽  
Acute Inflammatory Response ◽  
Reactive Protein ◽  
Antituberculosis Treatment ◽  
Tnf Α ◽  
Referral Hospitals ◽  
Ifn Γ

OBJECTIVE: To describe serum levels of the cytokines IL-10, TNF-α, and IFN-γ, as well as polymorphisms in the genes involved in their transcription, and their association with markers of the acute inflammatory response in patients with pulmonary tuberculosis.METHODS: This was a descriptive, longitudinal study involving 81 patients with pulmonary tuberculosis treated at two referral hospitals. We collected data on sociodemographic variables and evaluated bacteriological conversion at the eighth week of antituberculosis treatment, gene polymorphisms related to the cytokines studied, and serum levels of those cytokines, as well as those of C-reactive protein (CRP). We also determined the ESR and CD4+ counts.RESULTS: The median age of the patients was 43 years; 67 patients (82.7%) were male; and 8 patients (9.9%) were infected with HIV. The ESR was highest in the patients with high IFN-γ levels and low IL-10 levels. IFN-γ and TNF-α gene polymorphisms at positions +874 and −238, respectively, showed no correlations with the corresponding cytokine serum levels. Low IL-10 levels were associated with IL-10 gene polymorphisms at positions −592 and −819 (but not −1082). There was a negative association between bacteriological conversion at the eighth week of treatment and CRP levels.CONCLUSIONS: Our results suggest that genetic markers and markers of acute inflammatory response are useful in predicting the response to antituberculosis treatment.

scholarly journals Cytokine Polymorphisms, Their Influence and Levels in Brazilian Patients with Pulmonary Tuberculosis during Antituberculosis Treatment

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Eliana Peresi ◽  
Larissa Ragozo Cardoso Oliveira ◽  
Weber Laurentino da Silva ◽  
Érika Alessandra Pellison Nunes da Costa ◽  
João Pessoa Araujo ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Plasma Levels ◽  
Gene Polymorphisms ◽  
Essential Role ◽  
Active Tuberculosis ◽  
Mrna Levels ◽  
Tuberculosis Patients ◽  
Antituberculosis Treatment ◽  
Cytokine Levels ◽  
Tuberculosis Disease

Cytokines play an essential role during active tuberculosis disease and cytokine genes have been described in association with altered cytokine levels. Therefore, the aim of this study was to verify ifIFNG, IL12B, TNF, IL17A, IL10, and TGFB1gene polymorphisms influence the immune response of Brazilian patients with pulmonary tuberculosis (PTB) at different time points of antituberculosis treatment (T1, T2, and T3). Our results showed the following associations:IFNG+874 T allele andIFNG+2109 A allele with higher IFN-γlevels;IL12B+1188 C allele with higher IL-12 levels;TNF−308 A allele with higher TNF-αplasma levels in controls and mRNA levels in PTB patients at T1;IL17AA allele at rs7747909 with higher IL-17 levels;IL10−819 T allele with higher IL-10 levels; andTGFB1+29 CC genotype higher TGF-βplasma levels in PTB patients at T2. The present study suggests thatIFNG+874T/A,IFNG+2109A/G,IL12B+1188A/C,IL10−819C/T, andTGFB1+21C/T are associated with differential cytokine levels in pulmonary tuberculosis patients and may play a role in the initiation and maintenance of acquired cellular immunity to tuberculosis and in the outcome of the active disease while on antituberculosis treatment.

scholarly journals Routine laboratory parameters of newly diagnosed pulmonary tuberculosis patients: a single center study in Brazil

2020 ◽  
Vol 9 (11) ◽  
pp. e2559119873
Author(s):  
Ana Paula Biadola ◽  
André Aparecido dos Santos Correa ◽  
Amanda Aparecida Silva de Aguiar ◽  
Ualter Guilherme Cipriano Rosa ◽  
Paulo José Mascarenhas Mazaro ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Medical Records ◽  
Leukocyte Count ◽  
Hematological Parameters ◽  
Total Leukocyte Count ◽  
Clinical Prognosis ◽  
Tuberculosis Patients ◽  
Antituberculosis Treatment ◽  
Single Center Study ◽  
Using Data

Objective: Evaluate the severity of tuberculosis and its association with laboratory tests before antituberculosis treatment. Methodology: Patients with pulmonary tuberculosis were evaluated for severity using data available in medical records (Clinical; Excretion of mycobacteria; and Diagnostic imaging) and associated with biochemical and hematological parameters requested at the time of diagnosis and before antituberculosis treatment. Results: The evaluation of the severity showed a predominance of smear AFB (+), fever associated with other symptoms and infiltrative tuberculosis. Only a positive correlation between mycobacteria excretion and imaging diagnosis (rho=0.47; p=0.023) was observed. The patients presented hyponatremia (9/21), hypomagnesemia (6/13), and hypercalcemia (1/13), unrelated to the severity. The presence of fever influenced the number of non-segmented neutrophils (p=0.0142) and the total leukocyte count correlated with the increase in total neutrophils (rho=0.9631; p<0.0001). Conclusion: Different severity characteristics of tuberculosis, although they reflect the degree of commitment of the patient, are not always correlated; the evaluation of electrolytes could contribute to clinical behavior; and non-segmented neutrophils are associated with a worse clinical prognosis in patients with tuberculosis.

scholarly journals Prediction of Treatment Outcome with Inflammatory Biomarkers after 2 Months of Therapy in Pulmonary Tuberculosis Patients: Preliminary Results

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 789
Author(s):  
Simona Stefanescu ◽  
Relu Cocoș ◽  
Adina Turcu-Stiolica ◽  
Elena-Silvia Shelby ◽  
Marius Matei ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Characteristic Curve ◽  
White Blood Cells ◽  
Inflammatory Biomarkers ◽  
C Reactive Protein ◽  
Albumin Ratio ◽  
Tuberculosis Patients ◽  
Preliminary Results ◽  
Reactive Protein ◽  
Antituberculosis Treatment

Pro-inflammatory mediators play an important role in the pathogenesis of pulmonary tuberculosis. Consecutively, 26 pulmonary tuberculosis patients were enrolled in our study based on the exclusion criteria. We have used Spearman’s correlation analysis, hierarchical clustering and regression modelling to evaluate the association of 11 biomarkers with culture status after antituberculosis treatment. The results of our study demonstrated that six inflammatory biomarkers of 11, C-reactive protein (CRP), white blood cells (WBC), neutrophils, interferon gamma inducible protein 10, C-reactive protein (CRP) to albumin ratio (CAR) and neutrophil to albumin ratio (NAR), were significantly associated with culture negativity. The predictive ability of a composite model of seven biomarkers was superior to that of any single biomarker based on area under the receiver operating characteristic curve (AUC) analysis, indicating an excellent prediction efficacy (AUC:0.892; 95% CI:0.732-1.0). We also found that the highest significant trends and lower levels of CRP and IP-10 were observed in the two-month treated tuberculosis (TB) patients. We believe that our study may be valuable in providing preliminary results for an additional strategy in monitoring and management of the clinical outcome of pulmonary tuberculosis. Using a panel of predictors added a superior value in predicting culture status after anti-TB therapy.

scholarly journals Detection of Serum Levels of IL-17 and CCL-5 in a Sample of Iraqi Pulmonary Tuberculosis Patients

2021 ◽  
pp. 2887-2893
Author(s):  
Entssar S. Hafid ◽  
May K. Ismael
Keyword(s):  
Pulmonary Tuberculosis ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Interleukin 17 ◽  
Cell Trafficking ◽  
Tuberculosis Patients ◽  
Cytokines And Chemokines ◽  
Immune Tissues ◽  
The Mean

      Cytokines and chemokines are small-secreted proteins involved in many aspects of cell development, differentiation, and activation functions. A prominent characteristic of these molecules is their effect on the immune system in relation to the development of cell trafficking and immune tissues and organs. Furthermore, they play an important role in initiating and coordinating the organized and sequential recruitment and activation of cells into Mycobacterium tuberculosis-infected lungs. We aimed to evaluate the levels of interleukin -17 (IL-17) and the chemotactic chemokine (C-C motif) ligand 5 (CCL5) in the sera of pulmonary tuberculosis (PTB) patients. About 90 subjects were included, involving 50 patients with pulmonary TB and 40 apparently healthy individuals who were selected as a control group. Sera were obtained for measuring IL-17 and CCL-5 levels by enzyme linked immunosorbent assay (ELISA). The results revealed that serum levels of IL-17 showed no significant differences between each patient's group and control. In contrast, the serum level of CCL-5 was significantly increased in pulmonary tuberculosis patients compared to control (P ≤0.01). The mean ±SE values of IL-17 level in PTB patients and controls were 43.06 ±3.64 and 41.009 ± 0.009 pg/ml, respectively. While, the mean ±SE values of CCL-5 level in PTB patients and controls were 455.40 ±25.35 and 80.86 ± 5.96 ng/L, respectively. The results of the current study suggest that high levels of CCL-5 in the sera of PTB patients may indicate an important role in the immunopathogenesis of the disease. Therefore, this chemokine could be considered as a useful biomarker for the severity of PTB infections.

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