scholarly journals PSV-39 The relationship between structural integrity and sperm survival during hypothermic storage of stallion sperm

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 335-336
Author(s):  
Alexander Zaitsev ◽  
Elizaveta Bragina ◽  
Mikhail Atroshchenko ◽  
Valerii Kalashnikov ◽  
Anastasiya Nezalenova ◽  
...  

Abstract The aim of this work was to study the relationship between ultrastructural integrity and sperm survival during hypothermic storage of chilled and cryopreserved stallion sperm. Semen from 37 stallions, were collected during the breeding season. The structural integrity of sperm was studied using a Hitachi 700 electron microscope. Progressive motility was evaluated using an Olympus BX 41 phase contrast microscope. Sperm survival was defined as the ability of spermatozoa to maintain progressive motility above 5% during hypothermic (+4C) storage of sperm. Spearman nonparametric correlation analysis was used. Sperm survival at a temperature +4°C in chilled semen averaged 115.4±11.47 hours. After freezing sperm survival decreased on average to 48.9±5.56 hours (P < 0.001). A reliable correlation between the number of sperm with intact heads and sperm survival at a temperature of + 4°C in diluted (r=0.60; P < 0.001) and thawed (r=0.63; P < 0.001) sperm was established. A significant negative correlation between the number of spermatozoa with degradation of the acrosome in native sperm and their survival (r=-0.48; P < 0.01) under the hypothermic storage was found. For cryopreserved semen, a similar dependence has not been established. A significant correlation between the number of spermatozoa with the normal position of the acrosome and the sperm survival rate in native sperm was also established (r=0.52; P < 0.01). A significant negative correlation between sperm with acrosome hypoplasia and sperm survival was found (r=-0.67; P < 0.001). We assume that the structural integrity and ability of spermatozoa to maintain progressive motility for a long time under conditions of hypothermic storage depends on the quality of spermatogenesis and the resistance of cells to low and ultra-low temperatures. Authors acknowledge financial support from Russian Science Foundation, Grant No: 20-16-00101, development program of Bioresource collections “Cryobank of genetic recourses the All-Russian Research Institute for Horse Breeding.”

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 309-310
Author(s):  
Michail Atroshchenko ◽  
Elena Nikitkina ◽  
Valerii Kalashnikov ◽  
Aleksander Zaitcev ◽  
Valentina Zvyagina ◽  
...  

Abstract The aim of the work was to study the relationship between biochemistry of seminal plasma with the ejaculate volume and the concentration of spermatozoa. Ejaculates from 35 stallions aged 5 to 15 were collected during the breeding season (February–May). The concentration of calcium (Ca), phosphate (P), potassium (K), magnesium (Mg), selenium (Se), zinc (Zn) and lithium (Li) in seminal plasma were determined by atomic emission and mass spectrometry (AES-ICP and MS-ICP) using an mass spectrometer Elan 9000 (Perkin Elmer, USA) and an atomic emission spectrometer Optima 2000V (Perkin Elmer,USA). The concentration of glucose, phospholipids, aspartate-amino-transferase (AST) and alkaline phosphatase (AIP) was determined by ChemWell 2902V analyzer (Awareness Technology, USA). The significance of differences between the indices was determined using Student’s t-test, the differences at P ≤ 0.05 were considered statistically significant. A significant negative correlation between the volume of ejaculate and concentration of Se (r = -0.42; P = 0.01), P (r =-0.39; P = 0.001), AIP (r = -0.48; P = 0.01), Zn (r = -0.40; P = 0.01), Li (r = -0.39; P = 0.02), phospholipids (= -0.52; P = 0.03) in seminal plasma was established. A correlation between the concentration of sperm and the concentration of P (r = 0.71; P = 0.01), Se (r = 0.39; P = 0.02), Li (r = 0.33; P = 0.05), AIP (r = 0.81; P = 0.001) in seminal plasma was established. Also a negative correlation between the concentration of sperm and glucose (r = -0.62; P = 0.01) was found. We assumed that selenium, zinc, phosphorus, lithium, phospholipids and AIP in seminal plasma have an epididymal origin and do not depend on the secretion of the accessory sex glands. Glucose enters the ejaculate with the secrets of the accessory sex glands. Authors acknowledge financial support from Russian Science Foundation, Grant No: 17-16-01109, development program of Bioresource collections “Cryobank of genetic recourses the All-Russian Research Institute for Horse Breeding.”


SLEEP ◽  
2021 ◽  
Vol 44 (Supplement_2) ◽  
pp. A153-A154
Author(s):  
Huisu Jeon ◽  
Sonhye Jeoung ◽  
Goeun Kim ◽  
Hyeyoung An ◽  
Hyojin Nam ◽  
...  

Abstract Introduction Bedtime Procrastination (BP) is defined as the behavior of going to bed later than intended, despite the absence of external factors. Bedtime procrastination is also prevalent among insomnia patients, and is associated with various sleep problems. Recent studies suggest emotional regulation as a mechanism of the procrastination behavior that is the conceptual foundation of bedtime procrastination. Emotional regulation difficulties are also associated with insomnia, but there is still a lack of research on the relationship between insomnia, emotional regulation strategies and bedtime procrastination. Thus, the study assumed that severity of insomnia would affect bedtime procrastination, and examined the moderating effect of the emotional regulation strategies in this relationship. Methods This study was conducted in 376 adults (mean age 23.73 ±2.14 years, 84.6% females). Participants were asked to answer Bedtime procrastination scale (BPS), an emotional regulation strategy checklist, and the Insomnia severity scale (ISI). Results As a result, a significant positive correlation was found between insomnia severity and bedtime procrastination (r=.286, p<.01), and avoidant/distractive regulation style (r=.101, p<.05). active regulation style (r=-.172, p<.01) and support seeking regulation style (r=-.102, p<.01) showed a significant negative correlation with the severity of insomnia. Bedtime procrastination behavior showed significant negative correlation only with active regulation style (r=-.151, p<.01). Support seeking regulation style moderated the relationship between insomnia and bedtime procrastination behavior (B=.0165, 95%, CI=.0014, .0316). The interaction effect between insomnia and support seeking regulation style was also significant (∆R^2=.0112, p<.05), indicating that the effect of insomnia on bedtime procrastination depends on the level of use of the support seeking regulation style. Conclusion These findings suggest that the level of support seeking regulation style is meaningful in terms of how insomnia affects bedtime procrastination. Support (if any) This work was supported by the Ministry of Education of the Republic of Korea and the National Research Foundation of Korea(NRF-2018S1A5A8026807)


2021 ◽  
Vol 28 (Supplement_1) ◽  
Author(s):  
K Popov ◽  
A Novitskiy ◽  
Y Shvarts

Abstract Funding Acknowledgements Type of funding sources: None. Background. Many patients with COVID-19 suffer from arterial hypertension and have marked variations in blood pressure (BP) during the acute period of infection. The reasons for such changes are poorly understood. Purpose. To assess the relationship between the level of BP and the severity of changes in the main clinical and laboratory parameters in patients with arterial hypertension and with a moderate course of COVID-19 at the stage of the height of the disease. Materials and methods. The study included 86 hospitalized patients with verified coronavirus disease of moderate severity: 46 of them were male, the median age was 65 years. Patients received adequate hypertension therapy for a long time. After hospitalization, treatment was adjusted if necessary. The level of laboratory parameters in the acute period of the disease was determined, including the electrolytes, C-reactive protein (CRP), albumin, ferritin, general blood test, etc. BP measured daily. The relationship between laboratory parameters and BP level was evaluated by nonparametric correlation using the Spearman coefficient and multivariate analysis based on the "generalized nonlinear model". Results. In 67.44% of patients with hypertension, an elevated BP level (>140/90) was observed against the background of moderate COVID-19. There was a significant negative correlation between the levels of CRP and BP throughout the stay in the hospital, the most significant is the 5th day of hospitalization. At the same time, a slightly stronger relationship is observed with diastolic BP (r = -0.4425) than with systolic BP (r = -0.3525). A similar trend is observed in all BP measurements. Besides, a negative correlation was found between potassium levels and BP values. The strongest association of potassium levels is with systolic BP on the day of hospitalization (r = -0.4850) and diastolic pressure on the 1st day of hospitalization (r = -0.3561). Multivariate analysis confirmed independent and reliable "influence" of CRP (p = 0.00018) and potassium (p = 0.03921) as independent predictors of diastolic pressure. Conclusions. Most patients with hypertension and moderate COVID-19 have elevated BP levels. There is a statistically significant negative relationship between the values of BP and levels of CRP and potassium. The obtained data may correspond to the existing hypothesis about the significant role of the kinin–kallikrein system in the pathogenesis of COVID-19.


2021 ◽  
Vol 49 (4) ◽  
pp. 1-10
Author(s):  
Min Li ◽  
Ziyu Zhao ◽  
Lijuan Cui

We examined the relationships among psychological empowerment, emotional labor strategies, and job burnout of frontline hotel employees with reference to conservation of resources theory and the job demands–resources model. A sample of 414 employees from a Chinese chain hotel was recruited to take part in this study. There was a significant negative correlation between psychological empowerment and job burnout for these frontline hotel employees. Furthermore, the emotional labor strategies of deep acting and automatic regulation played partial mediating roles in the relationship between psychological empowerment and job burnout, which has implications for the development of intervention mechanisms and work practices for addressing burnout.


2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Linhong Wang ◽  
Yan Ruan ◽  
Jianping Chen ◽  
Yunxiao Luo ◽  
Fan Yang

AbstractThis study aimed to noninvasively assess the relationship between the labial gingival thickness (GT) and the underlying bone thickness (BT) of maxillary anterior teeth by two digital techniques. A total of 30 periodontally healthy participants with 172 maxillary anterior teeth were enrolled. GT and BT were measured at 2, 4 and 6 mm apical to the cemento-enamel junction (CEJ) by two digital techniques: M1—cone-beam computed tomography (CBCT) and M2—digital intraoral scanning (DIS) combined with CBCT. The Pearson's correlation coefficient was calculated to determine the correlation between GT and BT. A significant negative correlation was identified between GT and BT at 2 mm apical to the CEJ for central incisors (CI), lateral incisors (LI), and canines (CA) both by M1 and M2, while a weak negative correlation at 4 mm apical to the CEJ was observed by M1 for CA. No significant correlation was found at other sites by both M1 and M2. The labial BT was < 1 mm in most cases (85% of CI; 97% of LI; and 90% of CA). Within the limitation of this study, it was concluded that GT and BT seemed to be negatively correlated at 2 mm apical to the CEJ. Therefore, caution is warranted when implant restoration at the esthetic area of the anterior teeth.


2020 ◽  
Vol 13 (2) ◽  
pp. 96
Author(s):  
Odeh Suleiman Murad

People with social anxiety demonstrate different behavioral aspects such as nervousness, apprehension, fear, and concern that negatively affect their personal traits and self-esteem. The current study is designed to identify the relationship between social anxiety and self-esteem among university students. To achieve this purpose, the descriptive correlation approach was utilized. Social anxiety and self-esteem scales were applied to a sample of 334 university students in Jordan. The study results revealed a low level of social anxiety and a high level of self-esteem among participants. In addition, there was a statistically significant negative correlation between the level of social anxiety and self-esteem among participants. The study recommended holding workshops and seminars for fresh students at the beginning of each academic year, to alleviate their feeling of stress and social anxiety and to raise the level of their self-esteem.


Author(s):  
Mohammed Qaed Zaid Alghamdi

This study aims to identify the relationship between suicidal tendencies and psychological pressures for a sample of secondary school students and university students in Riyadh city. It also aims to reveal the differences in the suicidal tendencies level and psychological pressures in light of the following variables (education stage - parents' educational level - family's economic level). This research is applied to a sample of (100) secondary school students and (100) university students in Riyadh city. The researcher applied the following scales to the study sample members: suicidal tendencies scale prepared by Alshehri, Mohammed (2010), psychological pressures scale prepared by Alshawi, Suliman (2010) regulated to Saudi environment. The study findings revealed that there was a statistically significant positive correlation between psychological pressures and suicidal tendencies; there were statistically significant differences between the average score of secondary school students and university students in terms of suicidal tendencies in favor of the university students; there was a statistically significant negative correlation between the level of household's income and suicidal tendencies; there was a statistically negative correlation between father's educational level and suicidal tendencies; there was a statistically negative correlation between mother's educational level and suicidal tendencies. The research concluded a combination of recommendations that reduce the psychological pressures and suicidal tendencies for secondary school and university students. It also recommended researching the factors that increase the psychological pressures and suicidal tendencies for secondary school and university students. It also suggested providing proper solutions to create a suitable environment that reduces the psychological pressures and suicidal tendencies for secondary school and university students.


2018 ◽  
Vol 9 (4) ◽  
pp. 113
Author(s):  
Souzan Abd El-Menem Abd El-Ghafar Harfus ◽  
Amal Awad Abd El-Nabi Moussa ◽  
Samar Mabrook El-Nehrawy

Background and objective: Suicide still constitutes to be a critical and risky issue requiring preventive strategies. There is evidence to suggest that spirituality is vital to the process of discovering meaning in life and plays an important role in dealing with suicidal desire. The aim of this study was to investigate the relationship between spirituality and suicidal ideations among patients with major depressive disorder.Methods: Design and participants: A descriptive correlational design was utilized in the current study. A purposive sample of 181 patients with major depressive disorder was recruited. Setting: The study was conducted at inpatient psychiatric department of Tanta University and Neurology, Psychiatry, and Neuro-Surgery Center. Both hospitals are under the supervision and direction of the ministry of higher education. Tools: Four tools were used to collect data; socio-demographic and clinical characteristics structured interview schedule, Beck Depression Inventory, Daily Spiritual Experience Scale, and Scale for Suicide Ideation.Results: The current study indicated a statistical significant negative correlation between depression and spirituality. Also, a statistical significant negative correlation was found between spirituality and one subscale of suicide which is active suicide desire subscale. On the other side, there was a statistical significant positive correlation between depression and total score of suicide.Conclusions: Higher levels of spirituality may help buffer risk of active suicide desire and promote protective effect against depression. Recommendation: interventions that aim at increasing spiritual involvement and practice may be beneficial in reducing depressive symptoms and suicide desire.


2007 ◽  
Vol 101 (3_suppl) ◽  
pp. 1016-1022 ◽  
Author(s):  
Amanda M. A. Melton ◽  
Stefan E. Schulenberg

Logotherapy, developed by Viktor Frankl, posits that when one lacks meaning in life, boredom can result. Thus, the two constructs should be inversely related. To examine this relationship, 279 students ( M = 19.8 yr., SD = 2.6; 179 women, 98 men) from a university in the southern United States were administered the Purpose of Life test and the Boredom Proneness Scale. As expected, a statistically significant negative correlation was found between the scores on the two scales ( r = -.71). Directions for research are offered.


2014 ◽  
Vol 4 (2) ◽  
pp. 187-208 ◽  
Author(s):  
Xiaobao Song ◽  
Wenjia Zheng

Purpose – The purpose of this paper is to examine securities analyst independence in China's capital market and the effect on analyst independence of institutional investors’ shareholding and separation between control rights and cash flow rights of ultimate controller. Design/methodology/approach – Using data of China's listed companies from 2006 to 2012, the authors empirically tested the relationship between analyst following and volatility of stock return. And based on the test, the authors investigated the role played by institutional investors’ ownership and separation between control rights and cash flow rights of ultimate controller. Findings – According to the empirical results, there is a significant negative correlation between analyst following and volatility of stock return. Also, shareholding of institutional investors and the separation between control rights and cash flow rights of ultimate controllers will have an impact on the relationship between analyst following and volatility of stock return. When institutional investors hold higher proportion or the separation between control rights and cash flow rights of ultimate controllers keeps at a high level, the negative correlation between analyst following and volatility of stock return will weaken. Originality/value – First, based on the theory of market intermediation, the paper examined analyst independence by investigating and analyzing the relationship between analyst following and volatility of stock return. Second, it analyzed the factors affecting analyst independence by integrating enterprise characteristic variable and market characteristic variable on the basis of introducing two variables – shareholding of institutional investors and the separation between control rights and cash flow rights of ultimate controllers.


Sign in / Sign up

Export Citation Format

Share Document