Evaluating the utility of a CO2 surgical laser for piglet castration to reduce pain and improve wound healing: a pilot study

Abstract CO2 surgical lasers are widely used for procedures in veterinary and human medicine. There is evidence to suggest surgery using a CO2 laser reduces pain and swelling and improves healing time compared with surgery with a scalpel. Millions of piglets in North America are surgically castrated each year using a scalpel. Therefore, piglet welfare may be improved by making refinements to the surgical procedure. The objectives of this preliminary study were to determine the ability of a CO2 surgical laser to (1) reduce pain and (2) improve wound healing of piglets undergoing surgical castration. Two-day-old male Yorkshire × Landrace piglets were used and randomly assigned to 1 of 3 treatments (n = 10 piglets/treatment group): surgical castration with the CO2 laser, surgical castration with a scalpel, or sham (uncastrated control). Piglets were video recorded in their pens for 1 hr preprocedure and from 0 to 2, 6 to 8, and at 24 hr postprocedure for behavior scoring. Surgical site images were collected at baseline, 0, 8, 24, 48, 72, 96, 120, 144, and 168 hr postcastration for wound healing assessment. Infrared thermography images of the surgical site were also taken at baseline, 0, 0.5, 8, and 24 hr postprocedure to assess inflammation. Finally, blood was collected from each piglet at baseline and 0.5 hr postcastration to assess cortisol levels, prostaglandin E metabolite and pig-major acute phase protein concentration. Laser-castrated piglets displayed more pain behaviors across the observation period than scalpel-castrated piglets (P = 0.05). Laser-castrated piglets also displayed significantly more agonistic behavior than both scalpel-castrated piglets (P = 0.005) and sham piglets (P = 0.036); yet, laser-castrated piglets had significantly lower temperatures at the site of incision compared with scalpel-castrated piglets (P = 0.0211). There was no significant difference in wound healing or any of the blood parameters assessed between laser-castrated and scalpel-castrated piglets. There was evidence of thermal tissue damage on the scrotum of piglets that were castrated using the CO2 laser. This may have resulted in the unremarkable healing time and the increased pain behavior observed in this study. The surgical laser technique should be refined before conclusions can be made regarding the utility of a CO2 laser for piglet castration.

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Comparison between Conventional Microdissection and CO2 Laser in the Treatment of Vocal Cord Polyp

Bangladesh Journal of Otorhinolaryngology ◽

10.3329/bjo.v27i1.53201 ◽

2021 ◽

Vol 27 (1) ◽

pp. 17-24

Author(s):

Mohammad Wahiduzzaman ◽

Sayed Farhan Ali Razib ◽

Afroja Hossain ◽

Md Idrish Ali ◽

Md Habibur Rahman ◽

...

Keyword(s):

Vocal Cord ◽

Co2 Laser ◽

Visual Analysis ◽

Preoperative Assessment ◽

Laser Technique ◽

Postoperative Assessment ◽

Glottic Closure ◽

Vocal Cord Polyp ◽

Significant Difference ◽

Duration Of Surgery

Background: Vocal cord polyp usually arises from the epithelium and the lamina propria. Minimally invasive dissection procedures are employed to treat these vocal cord polyp for an effective outcome. Two types of microsurgical techniques were developed gradually and practiced namely, the conventional laryngeal microsurgery, which involves the use of cold instruments and the laryngeal laser micro-surgery. Objective: To compare the conventional cold dissection and CO2 laser methods in treatment of vocal cord polyp. Methods: A randomized prospective study was conducted at the Department of Otolayngology- Head & Neck Surgery, at Bangabondhu Sheikh Muzib Medical University (BSMMU) between July 2017 and June 2019. A total of 60 cases were studied on the following parameters: a. Visual analysis on stroboscopy b. Voice analysis –GRBAS (grade, roughness, breathiness, asthenia, strain) indices c. Duration of surgery d. Peroperative bleeding. Results: During first postoperative assessment, both groups had the normal symmetrical waves, while 50% of cases still had aperiodicity but all patients had the near normal periodicity in the second postoperative assessment. Regarding glottic closure, during preoperative assessment, almost all patients had incomplete glottic closure due to mass lesion as polyps. During first postoperative assessment, glottic closure was found to be better in group B (83% of cases) than group A (76% of cases), while during second postoperative assessment, all patients of both groups had around 95% glottic closure. The mean duration oflaser technique (7.1 ± 1.1 minutes) was less than the conventional technique time (15.6 ± 1.9 minutes), also mean of operative bleeding of laser technique (zero pack) is less than the conventional two(2 packs). Conclusion: There is no significant difference between both groups in all parameters except operative time and bleeding, as laser technique has less time and clear field. Bangladesh J Otorhinolaryngol; April 2021; 27(1): 17-24

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Effect of Curcuma longa Crude Extract, Curcumin and Nano Curcumin-based Gel Topical Administration on Excised Skin Wound Healing in Swiss-Webster Mice

Biomedical & Pharmacology Journal ◽

10.13005/bpj/2165 ◽

2021 ◽

Vol 14 (02) ◽

pp. 635-641

Author(s):

Afiat Berbudi ◽

Ali Akbar Fadlillah ◽

Merry Afni ◽

Nur Atik

Keyword(s):

Wound Healing ◽

Topical Application ◽

Curcuma Longa ◽

Area Under The Curve ◽

Topical Administration ◽

Treated Group ◽

Healing Time ◽

Control Group ◽

Skin Wound Healing ◽

Significant Difference

Patients suffering from skin wounds often have their life heavily affected economically and socially. In general, wound healing consists of four different sequentially and overlapping phases. Interrupting those phases results in extending the healing time. Turmeric (Curcuma longa) is widely used by Indonesians to treat wounds, with curcumin as an active substance, which is proven to have various effects on improving wound healing. Therefore, this study aims to determine how the topical application of turmeric gel on affects the acceleration of wound closure. Analytical laboratory experiments were conducted on 24 male Swiss-Webster mice. Subjects were divided into four groups according to where the excision wounds were made on the back, and observed on days 0, 3, 6, 9, 12, and 15 post wound induction. The wound healing acceleration was observed after 3 (p = 0.0003), 6 (p = 0.0085), and 9 (p = 0.0023) days in C. longa-treated mice compared to the control group. However, there was no significant difference found on days 12 and 15 after wounding. In addition, the area under the curve analysis shows significant difference between the C. longa-treated group compared to the control, but not with the group treated with neither curcumin nor nano curcumin gel. In conclusion, topical application of 3% C. longa extract gel improves excised skin healing.

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12. Effectiveness of Jatropha Sap Oinment 10% (Jatropha curcas Linn.) and Gentamicin Ointment 0.1% on Combustion Injury II Healing on Mice Skin (Mus musculus) (EFEKTIVITAS SALEP GETAH JARAK PAGAR 10% (Jatrophacurcas Linn.) DAN GENTAMISIN 0,1% TERHADAP PERCEPATAN PENYEMBUHAN LUKA BAKAR DERAJAT II PADA KULIT MENCIT (Mus musculus))

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v10i2.4631 ◽

2016 ◽

Vol 8 (1) ◽

Author(s):

Rodi Jasmadi

Keyword(s):

Wound Healing ◽

Key Words ◽

Analysis Of Variance ◽

Mus Musculus ◽

Jatropha Curcas ◽

Healing Process ◽

Healing Time ◽

Treatment Groups ◽

Significant Difference ◽

Difference Test

This research was aimed to determine the effectiveness of second degree combustion wound healing after the administration of Jatropha (Jatropha curcas Linn.) sap oinment 10% and Gentamicin ointment 0.1% on mice skin (Mus musculus). This research used 12 mice divided into 3 treatment groups: yellow vaseline lubrication (K0), jatropha sap oinment 10% (KI), and ointment Gentamicin 0.1% (KII). The parameter measured in this study was healing time (day) based on wound not visible/healed (diameter length=0 cm). Data of wound healing were analyzed using analysis of variance (Anova) and continued with least significant difference test. The time of wound healing on P0, P1, and P2 was 22.25, 17.75, and 20.25 days (P0.01). In conclusion, sap of jatropha oinment 10% is potensial to accelerate the combustion injury II healing process in mice. ____________________________________________________________________________________________________________________ Key words: wound, yellow vaseline, jarak pagar secretion 10%, gentamicin 0.1 %

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The Ethanol Extract of Neem Leaf (Azadirachta Indica A. Juss) Effect towards Wound Healing in Male Swiss Webster Mice

Journal Of Medicine & Health ◽

10.28932/jmh.v1i3.519 ◽

2016 ◽

Vol 1 (3) ◽

Author(s):

Hillary L. Mustamu ◽

Endang Evacuasiany ◽

Laella K. Liana

Keyword(s):

Experimental Study ◽

Wound Healing ◽

Azadirachta Indica ◽

Povidone Iodine ◽

Ethanol Extract ◽

Negative Control ◽

Healing Time ◽

Randomized Design ◽

Significant Difference

accelerates wound healing. We examined the effect of Ethanol Extract of Neem Leaf (EENL) inaccelerating wound healing and compared its potential with povidone iodine. This was ananalytic experimental study, using Complete Randomized Design (CRD) with a comparativemethod. We used 30 male Swiss Webster mice that were divided into five groups (n=6). Eachgroup was given the Ethanol Extract of Neem Leaf (EENL) with a concentration of 25%, 12.5%and 25%, aquades (negative control) and povidone iodine 10% (comparison). The averagewound healing time of EENL 25%, EENL 12.5% , EENL 6.25% , povidone iodine and aquadeswere 11.2; 12.2;13.2;13.2;and 1.3 days respectively. The data was analized with one wayANOVA and showed a highly significant difference of p=0.000. Tukey HSD test showed thatEENL 25% and 12.5% had better potential than povidone iodine 10% and EENL 6.25% had thesame potential with povidone iodine 10%. This study concludes that ethanol extract of neem leafhas an effect in accelerating the duration of wound healing, with EENL 25% as the bestconcentration in accelerating the duration of wound healing.Keywords: neem leaf, Azadirachta indica A. Juss, wound healing, Swiss Webster mice

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Comparison of Diode Laser and Conventional Method in Treatment of Gingival Melanin Hyperpigmentation

European Annals of Dental Sciences ◽

10.52037/eads.2021.0030 ◽

2021 ◽

Author(s):

Revan Birke Koca-Ünsal ◽

Gökhan Kasnak ◽

Erhan FIRATLI

Keyword(s):

Wound Healing ◽

Diode Laser ◽

Pain Perception ◽

Surgical Techniques ◽

Patient Comfort ◽

P Value ◽

Laser Technique ◽

Treatment Groups ◽

Significant Difference ◽

Laser Group

Purpose: The goal of this study is to compare the scalpel and diode laser methods for treating gingival hyperpigmentation in terms of postoperative discomfort and wound healing. Materials & Methods: Sixteen systemically healthy individuals diagnosed with light or moderate gingival hyperpigmentation were enrolled for this study. Patients were randomly assigned to one of two treatment groups: scalpel or laser. Dummett’s oral pigmentation index was recorded at baseline. Early wound healing and post-operative discomfort were evaluated on the 7th day by using the VAS form. Comparisons between the groups were tested using the Mann-Whitney U test and P-value < 0.05 was considered significant. Results: Total epithelization was observed in the laser group, whereas the epithelization was incomplete in the scalpel group at the end of the observation period. The pain perception on the first two days after the surgery was significantly higher in the scalpel group than in the laser group. (p=0,002 and p=0,038, respectively). No significant differences were found between the fourth- and seventh-day when surgical techniques were compared regarding pain perception (p>0,05). Similarly, pain perceptions of female and male individuals showed no significant difference (p>0,05). Conclusion: In the treatment of gingival hyperpigmentation, both surgical techniques were clinically successful. Although the treatment process was long, according to the results of our study, the laser technique is superior to the scalpel method in terms of patient comfort. The choice of the method may vary depending on the available equipment and the clinician’s preference or request of the patient.

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TYMPANOPLASTY TYPE-1: DIFFERENT APPROACHES AND THEIR SURGICAL OUTCOMES

Pakistan Armed Forces Medical Journal ◽

10.51253/pafmj.v1i1.7938 ◽

2021 ◽

Vol 71 (Suppl-3) ◽

pp. S495-99

Author(s):

Fazal I Wahid ◽

Abdul Manan ◽

Muhammad Saleem ◽

Bakht Zada ◽

Habib -Ur- Rehman ◽

...

Keyword(s):

Wound Healing ◽

Postoperative Pain ◽

Surgical Outcomes ◽

Neck Surgery ◽

Healing Time ◽

Cross Sectional ◽

Female Ratio ◽

Significant Difference ◽

Wound Healing Time

Objective: To calculate the surgical outcomes of different approaches of tympanoplasty type 1 in terms of air bone gap closure, wound healing, and postoperative pain. Study Design: Prospective cross-sectional study. Place and Duration of Study: Department of ENT, Head and Neck Surgery, Medical Teaching Institute (MTI), Lady Reading Hospital (LRH), Peshawar, Pakistan from Jun 2018 to May 2020. Methodology: Size of sample was 75 patients divided into three groups. Consent was obtained from all the patients included in study. After proper evaluation and relevant investigation pre- and post-operative PTA was performed. Tympanoplasty type-1 was performed by the same surgeon by the three approaches following established otological surgical protocol. Collected data was entered in SPSS-25 for analysis. Results: All 75 patients were divided into three equal groups. Male were 55 (73.3%), females were 20 (26.7%). Male: female ratio was 2.7:1. Majority of patients presented in 2nd and 3rd decades of life 31 (41.33%) and 28 (37.33%) respectively. Preoperative mean ± SD, ABG in groups A, B and C were 37.60 ± 11.28 dB, 37 ± 9.68 dB and 31.80 ± 12.40 dB, respectively. No significant difference was found amongst the three groups regarding pre-operative and post-operative ABG (p=0.85 and p=0.4), respectively. Mean ± SD, wound healing time in Groups A, B and C were 1.00 ± 0.00, 1.80 ± 0.40 and 1.76 ± 0.43 respectively with p=0.0001 and similarly postoperative pain with p=0.001. The wound healing time and post-operative was statistically better (p<0.05) in group A (Transmeatal approach) than other groups........

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Using a CO2 Surgical Laser for Piglet Castration to Reduce Pain and Inflammation, and to Improve Wound Healing

Kansas Agricultural Experiment Station Research Reports ◽

10.4148/2378-5977.8016 ◽

2020 ◽

Vol 6 (10) ◽

Author(s):

A. V. Viscardi ◽

C. A. Cull ◽

M. D. Kleinhenz ◽

S. Montgomery ◽

A. Curtis ◽

...

Keyword(s):

Wound Healing ◽

Improve Wound Healing ◽

Surgical Laser

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Effect of Methanolic Extract in Ointment and Powder of Kalanchoe Pinnata (Lamk) Leaf in Ointment towards Incision Wound Healing in Mice

Journal Of Medicine & Health ◽

10.28932/jmh.v1i1.495 ◽

2015 ◽

Vol 1 (1) ◽

Author(s):

Adi K Suprapto ◽

Fen Tih ◽

Endang Evacuasiany

Keyword(s):

Wound Healing ◽

Methanolic Extract ◽

Positive Control ◽

Negative Control ◽

Healing Time ◽

Accelerate Wound Healing ◽

Significant Difference ◽

Kalanchoe Pinnata ◽

Wound Healing Time ◽

Incision Wound

Wound is a very common thing that can happen to all people around the world. The purpose of this study is to investigate the effect of methanolic extract and powder of Kalanchoe pinnata (Lamk) leaf in ointment. The methodology is laboratoric experimental design. Six groups of mice (n=30) were incised for 8 mm and treated with different treatment: methanolic extract of Kalanchoe pinnata (Lamk) leaves in ointment (SEMDSB) 10%, SEMDSB 20%, Kalanchoe pinnata (Lamk) leaves powder in ointment (SSDSB) 10%, SSDSB 20%, vaseline album (ointment) as negative control, and povidone iodine ointment as positive control. Examination is carried out by measuring the width of wounds in mice every day until the wound interlocked. Data analysis used one way ANOVA continued by Tukey HSD with α=0,05. The result showed the average time for wound healing (in days) are: SEMDSB 10% (7.2), SEMDSB 20% (6.6), SSDSB 10% (8.8), SSDSB 20% (7.6), negative control (10.6), and positive control (7.8). SEMDSB 10%, SEMDSB 20%, and SSDSB 20% showed highly significant difference (p = 0,000) and SSDSB 10% showed significant difference (p=0,021) when compared to negative control. As conclusion, methanolic extract and powder of Kalanchoe pinnata (Lamk) leaves in ointment can accelerate wound healing time. Keywords: ointment, Kalanchoe pinnata (Lamk), wound healing, mice

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Assessment of wound area reduction on chronic wounds in dogs with photobiomodulation therapy: A randomized controlled clinical trial

Veterinary World ◽

10.14202/vetworld.2021.2251-2259 ◽

2021 ◽

pp. 2251-2259

Author(s):

Somphong Hoisang ◽

Naruepon Kampa ◽

Suvaluk Seesupa ◽

Supranee Jitpean

Keyword(s):

Wound Healing ◽

Adjunctive Therapy ◽

Chronic Wounds ◽

Photobiomodulation Therapy ◽

Wound Area ◽

Area Reduction ◽

Wound Size ◽

Significant Difference ◽

L1 And L2 ◽

Improve Wound Healing

Background and Aim: Chronic wounds are a clinical problem and require intensive standard wound care. However, this is sometimes insufficient to promote healing. Photobiomodulation therapy (PBMT) can be used as an adjunctive therapy to improve wound healing. Various PBMT devices with different properties and parameter settings as well as different animal species can influence a variety of clinical outcomes. This study aims to assess the use of 830 nm PBMT or simultaneous superpulsed and multiple wavelengths (SPMW; 660, 875, and 905 nm) PBMT on chronic wounds in client-owned dogs. Materials and Methods: This study included 21 client-owned dogs with chronic wounds allocated into three groups: (1) Control group (C) treated with irrigated saline and without PBMT (n=7); (2) L1 group treated with irrigated saline together with the radiation of 830 nm PBMT (n=7); and (3) L2 group treated with irrigated saline together with the radiation of simultaneous SPMW-PBMT (n=7). Wound healing was assessed on the basis of wound size reduction as a percentage of wound area every 2nd day for 15 days using image analysis software (ImageJ software®, National Institutes of Health, Rockville, Maryland, USA). Results: A significant difference in the percentage of wound area reduction was noted between the C and PBMT groups (L1 and L2; p<0.05). The average percentages of wound area reduction at the end of the study (15 days) were 42.39±20.58, 56.98±24.82, and 61.81±27.18 in the C, L1, and L2 groups, respectively. A steady decrease in wound size was noted in both PBMT and non-PBMT groups, and coefficients were 7.77, 8.95, and 10.01 in the C, L1, and L2 groups, respectively. The percentage of wound area reduction was found to be significantly different between the PBMT and non-BPMT groups on day 7 (p<0.05). Conclusion: Based on the results of the current study, using either 830 nm PBMT or simultaneous SPMW-PBMT can accelerate the chronic wound healing process in dogs with a significant reduction in wound area. Therefore, it can be used as an adjunctive therapy to improve wound healing in dogs with reduced treatment duration.

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PENGGUNAAN SELAPUT AMNION SEGAR PADA INSISI LUKA OPERASI SEKSIO SESAREA

JOURNAL OBGIN EMAS ◽

10.25077/aoj.1.2.47-55.2017 ◽

2019 ◽

Vol 1 (2) ◽

pp. 47-55

Author(s):

Syahredi Syaiful Adnani ◽

Hafni Bachtiar

Keyword(s):

Wound Healing ◽

Caesarean Section ◽

Amniotic Membrane ◽

Healing Time ◽

Cost Of Care ◽

Control Group ◽

P Value ◽

Control Groups ◽

And Control ◽

Wound Healing Time

In the last few decades, the incidence of caesarean section is increasing in the world, especially in Indonesia. One of the way to treat tissue scar is through biologic and synthetic dressing where nowadays, amnion has been used as biologic dressing frequently. This study was conducted to determine the effect of the use of fresh amniotic membrane on wound incision Caesarean section compared with Caesarean section incision wound covered using regular gauze bandages and fixated with plaster in RS. Dr. Reksodiwiryo Padang. The design of this study is an experimen-tal study with Post test design with control group design. Sampling was done using a formula consecutive sampling two different test samples obtained an average of 72 people for each group. The analysis used include univariate and bivariate analyzes. The average wound healing time the difference was statistically significant (p value <0.05) in the treatment and control groups. There was highly significant difference in the proportion of local infection on day 3 between the treatment and control groups (p value <0.05). There were very significant differences in the proportion of local allergic reactions at day 3, and 5 between the treatment and control group (p <0.05). There are significant differences in terms of the cost of care per day between treatment and control groups (p <0.05). From this study, the average wound healing time has a very significant difference.Keywords: Fresh Amniotic Membrane, Wound Cesarean Section, Wound Healing

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